A Agarwal

Summary

Affiliation: Cleveland Clinic Foundation
Country: USA

Publications

  1. ncbi request reprint Mechanism, measurement, and prevention of oxidative stress in male reproductive physiology
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, and Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Ohio, 44195, USA
    Indian J Exp Biol 43:963-74. 2005
  2. ncbi request reprint Relationship amongst teratozoospermia, seminal oxidative stress and male infertility
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Reprod Biol Endocrinol 12:45. 2014
  3. ncbi request reprint Characterizing semen parameters and their association with reactive oxygen species in infertile men
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio 44106, USA
    Reprod Biol Endocrinol 12:33. 2014
  4. pmc Potential markers for detection and monitoring of ovarian cancer
    Brandon J D Rein
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA
    J Oncol 2011:475983. 2011
  5. pmc A comprehensive review of genetics and genetic testing in azoospermia
    Alaa J Hamada
    Cleveland Clinic, Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland, Ohio, USA
    Clinics (Sao Paulo) 68:39-60. 2013
  6. pmc Oxidative stress and antioxidants for idiopathic oligoasthenoteratospermia: Is it justified?
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, USA
    Indian J Urol 27:74-85. 2011
  7. pmc Nonsurgical treatment of male infertility: specific and empiric therapy
    Marcello Cocuzza
    Reproductive Research Center, Glickman Urological and Kidney Institute and Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio, USA
    Biologics 1:259-69. 2007
  8. pmc Proteomic analysis of seminal fluid from men exhibiting oxidative stress
    Rakesh Sharma
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 11:85. 2013
  9. pmc An investigation of excess residual cytoplasm in human spermatozoa and its distinction from the cytoplasmic droplet
    Anil K Rengan
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Reprod Biol Endocrinol 10:92. 2012
  10. pmc Proteomic analysis of human spermatozoa proteins with oxidative stress
    Rakesh Sharma
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 11:48. 2013

Collaborators

Detail Information

Publications132 found, 100 shown here

  1. ncbi request reprint Mechanism, measurement, and prevention of oxidative stress in male reproductive physiology
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, and Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Ohio, 44195, USA
    Indian J Exp Biol 43:963-74. 2005
    ..We have also provided information on oxidative stress in other systems of the body, which may be applied to future research in the field of reproductive biology...
  2. ncbi request reprint Relationship amongst teratozoospermia, seminal oxidative stress and male infertility
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Reprod Biol Endocrinol 12:45. 2014
    ..Therefore, this study aimed to examine the relationship between seminal ROS production and sperm morphology in infertile teratozoospermic patients as well as in healthy men of proven and unproven fertility...
  3. ncbi request reprint Characterizing semen parameters and their association with reactive oxygen species in infertile men
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio 44106, USA
    Reprod Biol Endocrinol 12:33. 2014
    ..In addition, we sought to establish an ROS cutoff value in seminal plasma at which a patient may be predicted to be infertile...
  4. pmc Potential markers for detection and monitoring of ovarian cancer
    Brandon J D Rein
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA
    J Oncol 2011:475983. 2011
    ..Detection testing of biomarkers with long-term stability and combination panels of markers, will likely lead to effective screening strategies with high specificity and sensitivity for early detection of ovarian cancer...
  5. pmc A comprehensive review of genetics and genetic testing in azoospermia
    Alaa J Hamada
    Cleveland Clinic, Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland, Ohio, USA
    Clinics (Sao Paulo) 68:39-60. 2013
    ..We also present a critical appraisal of use of genetic testing in this subset of infertile patients...
  6. pmc Oxidative stress and antioxidants for idiopathic oligoasthenoteratospermia: Is it justified?
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, USA
    Indian J Urol 27:74-85. 2011
    ....
  7. pmc Nonsurgical treatment of male infertility: specific and empiric therapy
    Marcello Cocuzza
    Reproductive Research Center, Glickman Urological and Kidney Institute and Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio, USA
    Biologics 1:259-69. 2007
    ..Empiric medical therapy continues as a viable option. However, physicians and patients must understand that the success rates with any of the pharmacological therapies remain suboptimal...
  8. pmc Proteomic analysis of seminal fluid from men exhibiting oxidative stress
    Rakesh Sharma
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 11:85. 2013
    ..Proteomic profiling of seminal plasma proteins is important to understand the molecular mechanisms underlying oxidative stress and sperm dysfunction in infertile men...
  9. pmc An investigation of excess residual cytoplasm in human spermatozoa and its distinction from the cytoplasmic droplet
    Anil K Rengan
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Reprod Biol Endocrinol 10:92. 2012
    ..This review attempts to clarify excess residual cytoplasm's effect on fertility, examine the enzymes responsible, and suggest tests and possible treatment options for those affected by this defect...
  10. pmc Proteomic analysis of human spermatozoa proteins with oxidative stress
    Rakesh Sharma
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 11:48. 2013
    ..Significant alterations in the sperm proteome are associated with poor semen quality. The aim of the present study was to examine if elevated levels of reactive oxygen species cause an alteration in the proteomic profile of spermatozoa...
  11. pmc Lifestyle factors and reproductive health: taking control of your fertility
    Rakesh Sharma
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 11:66. 2013
    ..The growing interest and amount of research in this field have made it evident that lifestyle factors have a significant impact on fertility. ..
  12. pmc Functional proteomic analysis of seminal plasma proteins in men with various semen parameters
    Rakesh Sharma
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 11:38. 2013
    ..The objective of this study was to determine if analysis of differential expression of proteins in varying semen parameters can serve as potential biomarkers for male infertility...
  13. doi request reprint Effect of an isotonic lubricant on sperm collection and sperm quality
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Fertil Steril 99:1581-6. 2013
    ..To assess the influence of an isotonic lubricant used during sperm sample collection on [1] ease of collection and [2] resultant sperm quality...
  14. pmc Role of reactive nitrogen species in male infertility
    Sejal B Doshi
    Center for Reproductive Medicine, Cleveland Clinic, Euclid Avenue, Cleveland, OH, USA
    Reprod Biol Endocrinol 10:109. 2012
    ..This article describes both physiological and pathological roles of this unique subset of ROS...
  15. doi request reprint Insight into oxidative stress in varicocele-associated male infertility: part 1
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Nat Rev Urol 9:678-90. 2012
    ..These responses have their own implications in exacerbating the underlying oxidative stress and on the subsequent infertility...
  16. pmc The effects of oxidative stress on female reproduction: a review
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 10:49. 2012
    ....
  17. pmc Proteomics: a subcellular look at spermatozoa
    Stefan S du Plessis
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 9:36. 2011
    ..The aim of this study was to review the current literature on the various proteomic techniques, and their usefulness in diagnosing sperm dysfunction and potential applications in the clinical setting...
  18. pmc Cryopreservation/transplantation of ovarian tissue and in vitro maturation of follicles and oocytes: challenges for fertility preservation
    Alex C Varghese
    Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Ohio, USA
    Reprod Biol Endocrinol 6:47. 2008
    ..The scope of this article is to take a comprehensively look at the challenges still faced in order for these novel technologies to be routinely employed with the aim of successful fertility preservation...
  19. pmc Factors affecting the outcome of human blastocyst vitrification
    Amr A Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Ob Gyn and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:99. 2009
    ..The current information concerning safety and efficacy of the vitrification of blastocysts will be reviewed along with the variables that can impact the outcome of the procedure...
  20. pmc Pathophysiology of cell phone radiation: oxidative stress and carcinogenesis with focus on male reproductive system
    Nisarg R Desai
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics and Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:114. 2009
    ....
  21. pmc The association between leukocytes and sperm quality is concentration dependent
    Jakob E Lackner
    Department of Urology, KH Hietzing mit Neurologischem Zentrum Rosenhügel and Ludwig Boltzmann Institut für Andrologie und Urologie, Vienna, Austria
    Reprod Biol Endocrinol 8:12. 2010
    ..To evaluate the association between leukocytes (polymorphonuclear granulocytes -PMNL) and semen parameters at different leukocyte concentrations...
  22. ncbi request reprint Relationship between oxidative stress, varicocele and infertility: a meta-analysis
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, USA
    Reprod Biomed Online 12:630-3. 2006
    ..56-216.96, P < 0.00001). From the results, it can be concluded that there is increased oxidative stress in varicocele patients; however, more studies are needed to confirm this finding...
  23. ncbi request reprint Clinical relevance of oxidative stress in male factor infertility: an update
    Ashok Agarwal
    Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, OH 44195, USA
    Am J Reprod Immunol 59:2-11. 2008
    ..The benefits of incorporating antioxidants in clinical and experimental settings have been enumerated. We also highlight the emerging concept of utilizing OS as a method of contraception and the potential problems associated with it...
  24. ncbi request reprint Oxidative stress in an assisted reproductive techniques setting
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 86:503-12. 2006
    ..The impact of oxidative stress on ART outcome and the different strategies designed to prevent it are also discussed...
  25. pmc Role of oxidative stress in female reproduction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Reprod Biol Endocrinol 3:28. 2005
    ..Serial measurement of oxidative stress biomarkers in longitudinal studies may help delineate the etiology of some of the diosorders in female reproduction such as preeclampsia...
  26. ncbi request reprint Correlation of reactive oxygen species levels with the fertilization rate after in vitro fertilization: a qualified meta-analysis
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Fertil Steril 84:228-31. 2005
    ..We conclude that ROS has a statistically significant effect on the fertilization rate after IVF, and that the measurement of ROS levels in semen specimens before IVF may be useful in predicting the IVF outcome and in counseling patients...
  27. ncbi request reprint Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 89:124-8. 2008
    ..To investigate the effect of cell phone use on various markers of semen quality...
  28. ncbi request reprint Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute, Cleveland, Ohio 44195, USA
    Fertil Steril 87:156-62. 2007
    ..To evaluate the performance of the automated semen quality analyzer system for assessing sperm quality...
  29. doi request reprint Lifestyle and testicular dysfunction: a brief update
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obst Gyne and Women s Health Institute, Cleveland Clinic, Cleveland, OH, USA
    Biomed Pharmacother 62:550-3. 2008
    ....
  30. ncbi request reprint Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    BJU Int 95:503-7. 2005
  31. ncbi request reprint Implications of systemic malignancies on human fertility
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Reprod Biomed Online 9:673-9. 2004
    ..However, there are some ethical issues that should be considered before offering patients any of these options...
  32. ncbi request reprint Role of oxidative stress in the pathophysiological mechanism of erectile dysfunction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Ave, OH 44195, USA
    J Androl 27:335-47. 2006
  33. ncbi request reprint Current and future perspectives on intracytoplasmic sperm injection: a critical commentary
    Alex C Varghese
    Molecular Research and Investigation Centre, Kolkata, India
    Reprod Biomed Online 15:719-27. 2007
    ..Since new studies on ICSI are published regularly, it is important that the established protocol is revised often, and that the role of ICSI in infertility therapy is continually re-evaluated...
  34. ncbi request reprint Oxidative stress and its implications in female infertility - a clinician's perspective
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Department of Obstetrics Gynecology and Glickman Urological Institute, The Cleveland Clinic Foundation, OH 44195, USA
    Reprod Biomed Online 11:641-50. 2005
    ..This review discusses the sources of ROS in media used in IVF-embryo transfer and strategies to overcome OS in oocyte in-vitro maturation, in-vitro culture and sperm preparation techniques...
  35. ncbi request reprint What an andrologist/urologist should know about free radicals and why
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Cleveland, Ohio 44195, USA
    Urology 67:2-8. 2006
  36. ncbi request reprint Prevention of oxidative stress injury to sperm
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Androl 26:654-60. 2005
  37. ncbi request reprint Efficacy of varicocelectomy in improving semen parameters: new meta-analytical approach
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute, Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Urology 70:532-8. 2007
    ..To determine the efficacy of varicocelectomy in improving semen parameters...
  38. ncbi request reprint Reactive oxygen species as an independent marker of male factor infertility
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 86:878-85. 2006
    ..To determine the abnormal patterns of reactive oxygen species (ROS) production in male factor infertility (MFI) patients and to define the ROS reference values in such patients...
  39. ncbi request reprint The role of free radicals and antioxidants in reproduction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and the Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Curr Opin Obstet Gynecol 18:325-32. 2006
    ..This review summarizes the role of free radicals and oxidative stress in the pathophysiology of human reproduction...
  40. ncbi request reprint Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 89:375-9. 2008
    ..To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity...
  41. ncbi request reprint Sperm DNA damage assessment: a test whose time has come
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 84:850-3. 2005
    ..The available tests of sperm DNA damage require additional large-scale clinical trials before their integration into routine clinical practice...
  42. ncbi request reprint Emerging technologies for the molecular study of infertility, and potential clinical applications
    Alex C Varghese
    DeCode Life Foundation, Kolkata, India
    Reprod Biomed Online 15:451-6. 2007
    ..However, their widespread use in laboratories and clinics may not be seen until preliminary studies confirming their safety and effectiveness are published, and until standardized protocols for their utilization are established...
  43. doi request reprint Redox considerations in female reproductive function and assisted reproduction: from molecular mechanisms to health implications
    Ashok Agarwal
    Reproductive Research Center, Department of Obstetrics and Gynecology, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Antioxid Redox Signal 10:1375-403. 2008
    ..Although antioxidants such as folate, zinc, and thiols may help enhance fertility, the available data are contentious and must be evaluated in controlled studies with larger populations...
  44. doi request reprint Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: an in vitro pilot study
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 92:1318-25. 2009
    ..To evaluate effects of cellular phone radiofrequency electromagnetic waves (RF-EMW) during talk mode on unprocessed (neat) ejaculated human semen...
  45. doi request reprint Role of oxidative stress in pathogenesis of varicocele and infertility
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Urology 73:461-9. 2009
    ..We conclude that spermatozoal dysfunction in varicocele patients could be multifactorial, and oxidative stress-induced injury appears to be one of the main causes...
  46. doi request reprint Assessing sperm function
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological and Kidney Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Urol Clin North Am 35:157-71, vii. 2008
    ..Finally, promising new tests under development are mentioned with their potential clinical applications...
  47. ncbi request reprint Role of oxidants in male infertility: rationale, significance, and treatment
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Desk A 19 1, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Urol Clin North Am 29:817-27. 2002
    ..A double-blind, placebo-controlled study underway at the authors' center is evaluating the effects of treating infertile men with a combination of vitamins E and C after adjusting for all causes of controversy...
  48. ncbi request reprint New semen quality scores developed by principal component analysis of semen characteristics
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Androl 24:343-52. 2003
    ..The composite scores can summarize overall SQ and quantity. Both SQ and RQ scores provide meaningful information on the quality of semen specimens for the clinician...
  49. ncbi request reprint Role of reactive oxygen species in the pathophysiology of human reproduction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 79:829-43. 2003
    ..To summarize the role of reactive oxygen species (ROS) in the pathophysiology of human reproduction...
  50. ncbi request reprint Role of sperm chromatin abnormalities and DNA damage in male infertility
    A Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Hum Reprod Update 9:331-45. 2003
    ..Therefore, screening for sperm DNA damage may provide useful information in cases of male idiopathic infertility and in those men pursuing assisted reproduction. Treatment should include methods for prevention of sperm DNA damage...
  51. ncbi request reprint Evaluation of nuclear DNA damage in spermatozoa from infertile men with varicocele
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 80:1431-6. 2003
    ..To examine levels of sperm DNA damage and oxidative stress (OS) in infertile men with varicocele...
  52. doi request reprint The role of antioxidant therapy in the treatment of male infertility
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Ob Gyn and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Hum Fertil (Camb) 13:217-25. 2010
    ....
  53. pmc Potential biological role of poly (ADP-ribose) polymerase (PARP) in male gametes
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 7:143. 2009
    ..PARP could provide new strategies to preserve fertility in cancer patients subjected to genotoxic stresses and may be a key to better male reproductive health...
  54. ncbi request reprint Chemiluminescence technique for measuring reactive oxygen species
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Reprod Biomed Online 9:466-8. 2004
    ..A wide range of luminometers are available in the market, and laboratories should select the instruments that suit their individual needs...
  55. ncbi request reprint Carnitines and male infertility
    A Agarwal
    Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH 44195, USA
    Reprod Biomed Online 8:376-84. 2004
    ..Additional, well-designed studies are necessary to further validate the use of carnitines in the treatment of patients with male infertility, specifically in men with poor semen quality...
  56. pmc Vitrification of isolated mice blastomeres using a closed loading device
    Amr Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics and Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:17. 2009
    ..Using a cryotip and propagation in microvolumes, isolated mice blastomeres were vitrified and warmed with 100% post-warming survival...
  57. ncbi request reprint Role of free radicals in female reproductive diseases and assisted reproduction
    Ashok Agarwal
    Centre for Advanced Research in Human, Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH 44195, USA
    Reprod Biomed Online 9:338-47. 2004
    ..Research is in progress to identify the mechanisms that are involved in the aetiology of female reproductive diseases caused by ROS, and to create effective strategies that can counteract oxidative stress...
  58. ncbi request reprint Inter-sample variability in post-thaw human spermatozoa
    Kiran P Nallella
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Cryobiology 49:195-9. 2004
    ..Further research is necessary to understand the mechanism(s) responsible for this variability. This may also assist clinicians utilize semen samples with optimum semen quality in ART procedures...
  59. ncbi request reprint The effect of sperm DNA damage on assisted reproduction outcomes. A review
    A Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, OH 44195, USA
    Minerva Ginecol 56:235-45. 2004
    ..The results of this evaluation may be used to counsel the couple about their chances for live birth and for genomic abnormalities in their offspring...
  60. ncbi request reprint Role of antioxidants in treatment of male infertility: an overview of the literature
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Dept of Obstetrics and Gynecology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH, USA
    Reprod Biomed Online 8:616-27. 2004
    ..Multicentre, double-blind studies with statistically accepted sample size are still needed to provide conclusive evidence on the benefit of antioxidants as a treatment modality for patients with male infertility...
  61. ncbi request reprint DNA damage in patients with untreated cancer as measured by the sperm chromatin structure assay
    H Kobayashi
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Fertil Steril 75:469-75. 2001
    ..CONCLUSION(S): DNA damage in spermatozoa is prevalent in the majority of cancer patients. SCSA provides important information about the biochemical integrity of sperm DNA in men with cancer before their treatment...
  62. ncbi request reprint Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility
    M Ollero
    Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Hum Reprod 16:1912-21. 2001
    ..DNA damage in mature spermatozoa may be the result of oxidative damage by ROS-producing immature spermatozoa during sperm migration from the seminiferous tubules to the epididymis...
  63. ncbi request reprint Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, The Cleveland Clinic Foundation, Ohio 44195, USA
    Fertil Steril 78:491-9. 2002
    ..To investigate levels of seminal oxidative stress (OS) and sperm quality in a group of infertile men with a history of cigarette smoking...
  64. ncbi request reprint Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation
    E Gil-Guzman
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, The Cleveland Clinic Foundation, Cleveland, OH 44165, USA
    Hum Reprod 16:1922-30. 2001
    ....
  65. ncbi request reprint Varicocelectomy improves intrauterine insemination success rates in men with varicocele
    J A Daitch
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, Department of Gynecology-Obstetrics and Biostatistics, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    J Urol 165:1510-3. 2001
    ..A functional factor not measured on routine semen analysis may affect pregnancy rates in this setting. Men should be screened for varicocele before intrauterine insemination is initiated for male factor infertility...
  66. ncbi request reprint Oxidative stress in normospermic men undergoing infertility evaluation
    F F Pasqualotto
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, Cleveland, Ohio, USA
    J Androl 22:316-22. 2001
    ..We conclude that oxidative stress is associated with male factor infertility. The presence of oxidative stress in infertile normospermic men may explain previously unexplained cases of infertility otherwise attributed to female factors...
  67. ncbi request reprint Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic
    R K Sharma
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, Cleveland Clinic Foundation, Ohio 44195, USA
    J Androl 22:575-83. 2001
    ..Complete removal of WBCs from semen samples used for assisted reproduction may help reduce oxidative stress...
  68. doi request reprint A red palm oil diet can reduce the effects of oxidative stress on rat spermatozoa
    Y G Aboua
    Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, South Africa
    Andrologia 44:32-40. 2012
    ..We therefore propose that a daily intake of RPO supplement to the diet might be helpful in protecting males against the adverse effects of high ROS in sperm function and help preserve fertility...
  69. doi request reprint Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm characteristics
    A C Varghese
    Fertility Clinic and IVF Department, AMRI Medical Centre, Kolkata, India
    Andrologia 41:207-15. 2009
    ..Sperm assessment for DNA status using AO is reliable and shows good correlation with sperm count, motility and morphology. Assessment of sperm DNA status with AO staining may be helpful prior to ART...
  70. doi request reprint Evaluation of post-thaw DNA integrity of mouse blastocysts after ultrarapid and slow freezing
    Amr Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Fertil Steril 91:2087-94. 2009
    ..To evaluate the effect of vitrification and two other methods of slow cryopreservation on DNA integrity in expanded and nonexpanded blastocysts...
  71. ncbi request reprint Protein supplementation and the incidence of apoptosis and oxidative stress in mouse embryos
    Navid Esfandiari
    Department of Obstetrics and Gynecology, Glickman Urological Institute, Cleveland, Ohio 44195, USA
    Obstet Gynecol 105:653-60. 2005
    ..To estimate the effect of protein supplementation of culture media on reactive oxygen species production and incidence of apoptosis in preimplantation mouse embryos...
  72. ncbi request reprint Oxidative stress & male infertility
    Kartikeya Makker
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obsterics Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Indian J Med Res 129:357-67. 2009
    ..It also highlights the mechanism of production and the pathophysiology of ROS in relation to the male reproductive system and enumerate the benefits of incorporating antioxidants in clinical and experimental settings...
  73. ncbi request reprint Differential growth of human embryos in vitro: role of reactive oxygen species
    Mohamed A Bedaiwy
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Fertil Steril 82:593-600. 2004
    ..To examine the relationship of early human embryonic development with the level of reactive oxygen species (ROS) in the culture media on the first day (day 1 ROS) after insemination...
  74. doi request reprint Lipid peroxidation and antioxidant status in preeclampsia: a systematic review
    Sajal Gupta
    Glickman Urological and Kidney Institute, Cleveland, OH 44195, USA
    Obstet Gynecol Surv 64:750-9. 2009
    ..Preeclampsia is characterized by increased lipid peroxidation and diminished antioxidant capacity; however, there is no consensus as to the extent of these conditions...
  75. ncbi request reprint Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis
    Marcello Cocuzza
    Reproductive Research Center, Glickman Urological and Kidney Institute, Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Int Braz J Urol 33:603-21. 2007
    ..Thus evaluation of seminal ROS levels and extent of sperm DNA damage especially in an infertile male may help develop new therapeutic strategies and improve success of assisted reproductive techniques (ART)...
  76. ncbi request reprint Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay
    Juan G Alvarez
    Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    Fertil Steril 78:319-29. 2002
    ..To determine DNA damage as measured by the sperm chromatin structure assay (SCSA) in subsets of human spermatozoa at different stages of maturation in patients who are undergoing infertility evaluation...
  77. ncbi request reprint Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study
    Xia Wang
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 80:844-50. 2003
    ..To evaluate the mitochondrial membrane potential (MMP) of spermatozoa and its correlation with semen parameters and production of reactive oxygen species (ROS) in infertile men and healthy donors...
  78. ncbi request reprint Sperm DNA damage and its clinical relevance in assessing reproductive outcome
    R K Sharma
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH 44195, USA
    Asian J Androl 6:139-48. 2004
    ..In turn, this may alleviate the financial, social and emotional problems associated with failed ART attempts...
  79. ncbi request reprint Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures
    Eleonora B Pasqualotto
    Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 81:973-6. 2004
    ....
  80. ncbi request reprint Male gamete survival at stake: causes and solutions
    Alex C Varghese
    Reproductive Research Centre, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biomed Online 17:866-80. 2008
    ..This review aims to include all the possible causes of disruption to this unique cell and their probable solutions, in the hope of clearing up the ambiguity that surrounds male factor infertility...
  81. ncbi request reprint Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 78:1215-24. 2002
    ..Such stimulation may be mediated via direct cell-cell contact or by soluble products released by leukocytes. Poor sperm quality in leukocytospermic samples may be due to leukocyte-mediated oxidative stress...
  82. ncbi request reprint Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 79:1597-605. 2003
    ..To examine the effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Prospective study...
  83. ncbi request reprint Human sperm superoxide anion generation and correlation with semen quality in patients with male infertility
    Tamer M Said
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, OH 44195, USA
    Fertil Steril 82:871-7. 2004
    ..generation in infertile men and determine whether sperm quality is correlated with levels of ROS triggered by the exogenous reduced form of beta nicotinamide adenine dinucleotide phosphate (NADPH)...
  84. ncbi request reprint Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility
    Xia Wang
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Department of Obsterics and Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 80:531-5. 2003
    ....
  85. ncbi request reprint Free radicals: their beneficial and detrimental effects on sperm function
    Shiva Kothari
    Center for Reproductive Medicine, Cleveland Clinic, 9500 Euclid Avenue, Desk A19 1, Cleveland, Ohio 44195, USA
    Indian J Exp Biol 48:425-35. 2010
    ..Pathologically, free radicals induce lipid peroxidation, DNA damage and apoptosis of spermatozoa. The present review deals with both the beneficial and detrimental effects of free radicals on sperm function...
  86. ncbi request reprint Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility
    Mohamed H Moustafa
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Hum Reprod 19:129-38. 2004
    ..The aim of this study was to examine the role of apoptosis and reactive oxygen species (ROS) in inducing DNA damage in ejaculated spermatozoa...
  87. doi request reprint Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species
    Reda Mahfouz
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 94:2141-6. 2010
    ..To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS)...
  88. doi request reprint Poor semen quality and ROS-TAC scores in patients with idiopathic infertility
    Fabio F Pasqualotto
    Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Urol Int 81:263-70. 2008
    ....
  89. doi request reprint Present and future fertility preservation strategies for female cancer patients
    Elena S Georgescu
    Center for Reproductive Medicine, Cleveland Clinic, Ohio 44195, USA
    Obstet Gynecol Surv 63:725-32. 2008
    ..IVF with donor oocyte remains an established option with a very high success rate for those who fail to conceive with the above measures or who elect not to avail themselves to experimental procedures...
  90. ncbi request reprint Relationship of interleukin-6 with semen characteristics and oxidative stress in patients with varicocele
    Kiran P Nallella
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Urology 64:1010-3. 2004
    ..To examine levels of interleukin-6 (IL-6) in fertile semen donors and patients with varicocele and examine its association with semen characteristics and levels of reactive oxygen species (ROS)...
  91. ncbi request reprint Role of total antioxidant capacity in the differential growth of human embryos in vitro
    Mohamed Bedaiwy
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 86:304-9. 2006
    ..The objective of this study was to examine the relationship of early human embryonic development parameters with day 1 culture media total antioxidant levels (day 1 TAC)...
  92. ncbi request reprint Role of oxidative stress in endometriosis
    Sajal Gupta
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, OH, USA
    Reprod Biomed Online 13:126-34. 2006
    ..Results of the limited number of animal and human trials need to be corroborated by larger randomized controlled trials...
  93. ncbi request reprint Technical and ethical challenges of fertility preservation in young cancer patients
    Fnu Deepinder
    Reproductive Research Center, Glickman Urological and Kidney Institute and Department of Obstetrics Gynecology, The Cleveland Clinic, Cleveland, OH, USA
    Reprod Biomed Online 16:784-91. 2008
    ..This review summarizes the technical and ethical challenges of gamete cryopreservation in young cancer patients...
  94. ncbi request reprint Identification of male factor infertility using a novel semen quality score and reactive oxygen species levels
    Kiran P Nallella
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Cleveland, Ohio, USA
    Clinics (Sao Paulo) 60:317-24. 2005
    ..To determine whether patients with male factor infertility can be accurately identified by calculating a novel semen quality score and measuring levels of reactive oxygen species during routine infertility screening...
  95. pmc p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts
    M L Agarwal
    Department of Molecular Biology, Cleveland Clinic Foundation, OH 44195, USA
    Proc Natl Acad Sci U S A 92:8493-7. 1995
    ..Therefore, irreversible arrest must involve processes other than or in addition to the interaction of p53-induced p21/WAF1 with G1 and G2 cyclin-dependent kinases...
  96. ncbi request reprint Serum and peritoneal abnormalities in endometriosis: potential use as diagnostic markers
    S Gupta
    Reproductive Research Center, Glickman Urological Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Minerva Ginecol 58:527-51. 2006
    ..Potential new therapeutic agents include modulators, such as cytokine receptor blockers and angiogenic receptor blockers, presently used for treating endometriosis...
  97. ncbi request reprint Regulation of p53 expression by the RAS-MAP kinase pathway
    M L Agarwal
    Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio, OH 44195, USA
    Oncogene 20:2527-36. 2001
    ..We conclude that MAP kinase-dependent pathways help to regulate p53 levels by regulating the expression of p53 mRNA...
  98. ncbi request reprint Pathophysiology of varicoceles in male infertility
    C K Naughton
    Center for Advanced Research in Human Reproduction and Infertility, Department of Urology, The Cleveland Clinic Foundation, Ohio 44195, USA
    Hum Reprod Update 7:473-81. 2001
    ....
  99. ncbi request reprint Relationship of interleukin-6 with semen characteristics and oxidative stress in vasectomy reversal patients
    K C Nandipati
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Andrologia 37:131-4. 2005
    ..41, P = 0.05 versus r = 0.38, P = 0.15). We conclude that patients who undergo vasectomy reversal have elevated levels of IL-6 and ROS, which may contribute to decreased sperm motility and concentration and possibly lead to sub-fertility...
  100. doi request reprint Relationship of reactive oxygen species levels in day 3 culture media to the outcome of in vitro fertilization/intracytoplasmic sperm injection cycles
    Mohamed A Bedaiwy
    Department of Obstetrics and Gynecology, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio 44195, USA
    Fertil Steril 94:2037-42. 2010
    ..To examine the relationship of early human embryonic development parameters to day 3 reactive oxygen species (D-3 ROS) levels in culture media...