A Agarwal

Summary

Affiliation: Cleveland Clinic Foundation
Country: USA

Publications

  1. ncbi Mechanism, measurement, and prevention of oxidative stress in male reproductive physiology
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, and Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Ohio, 44195, USA
    Indian J Exp Biol 43:963-74. 2005
  2. ncbi Effect of an isotonic lubricant on sperm collection and sperm quality
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Electronic address
    Fertil Steril 99:1581-6. 2013
  3. ncbi Role of reactive nitrogen species in male infertility
    Sejal B Doshi
    Center for Reproductive Medicine, Cleveland Clinic, Euclid Avenue, Cleveland, OH, USA
    Reprod Biol Endocrinol 10:109. 2012
  4. ncbi Insight into oxidative stress in varicocele-associated male infertility: part 1
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Nat Rev Urol 9:678-90. 2012
  5. ncbi The effects of oxidative stress on female reproduction: a review
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 10:49. 2012
  6. ncbi Proteomics: a subcellular look at spermatozoa
    Stefan S du Plessis
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 9:36. 2011
  7. ncbi Cryopreservation/transplantation of ovarian tissue and in vitro maturation of follicles and oocytes: challenges for fertility preservation
    Alex C Varghese
    Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Ohio, USA
    Reprod Biol Endocrinol 6:47. 2008
  8. ncbi Factors affecting the outcome of human blastocyst vitrification
    Amr A Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Ob Gyn and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:99. 2009
  9. ncbi Pathophysiology of cell phone radiation: oxidative stress and carcinogenesis with focus on male reproductive system
    Nisarg R Desai
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics and Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:114. 2009
  10. ncbi The association between leukocytes and sperm quality is concentration dependent
    Jakob E Lackner
    Department of Urology, KH Hietzing mit Neurologischem Zentrum Rosenhügel and Ludwig Boltzmann Institut für Andrologie und Urologie, Vienna, Austria
    Reprod Biol Endocrinol 8:12. 2010

Collaborators

Detail Information

Publications119 found, 100 shown here

  1. ncbi Mechanism, measurement, and prevention of oxidative stress in male reproductive physiology
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, and Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Ohio, 44195, USA
    Indian J Exp Biol 43:963-74. 2005
    ..We have also provided information on oxidative stress in other systems of the body, which may be applied to future research in the field of reproductive biology...
  2. ncbi Effect of an isotonic lubricant on sperm collection and sperm quality
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio Electronic address
    Fertil Steril 99:1581-6. 2013
    ..To assess the influence of an isotonic lubricant used during sperm sample collection on [1] ease of collection and [2] resultant sperm quality...
  3. ncbi Role of reactive nitrogen species in male infertility
    Sejal B Doshi
    Center for Reproductive Medicine, Cleveland Clinic, Euclid Avenue, Cleveland, OH, USA
    Reprod Biol Endocrinol 10:109. 2012
    ..This article describes both physiological and pathological roles of this unique subset of ROS...
  4. ncbi Insight into oxidative stress in varicocele-associated male infertility: part 1
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Nat Rev Urol 9:678-90. 2012
    ..These responses have their own implications in exacerbating the underlying oxidative stress and on the subsequent infertility...
  5. ncbi The effects of oxidative stress on female reproduction: a review
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 10:49. 2012
    ....
  6. ncbi Proteomics: a subcellular look at spermatozoa
    Stefan S du Plessis
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 9:36. 2011
    ..The aim of this study was to review the current literature on the various proteomic techniques, and their usefulness in diagnosing sperm dysfunction and potential applications in the clinical setting...
  7. ncbi Cryopreservation/transplantation of ovarian tissue and in vitro maturation of follicles and oocytes: challenges for fertility preservation
    Alex C Varghese
    Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Ohio, USA
    Reprod Biol Endocrinol 6:47. 2008
    ..The scope of this article is to take a comprehensively look at the challenges still faced in order for these novel technologies to be routinely employed with the aim of successful fertility preservation...
  8. ncbi Factors affecting the outcome of human blastocyst vitrification
    Amr A Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Ob Gyn and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:99. 2009
    ..The current information concerning safety and efficacy of the vitrification of blastocysts will be reviewed along with the variables that can impact the outcome of the procedure...
  9. ncbi Pathophysiology of cell phone radiation: oxidative stress and carcinogenesis with focus on male reproductive system
    Nisarg R Desai
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics and Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:114. 2009
    ....
  10. ncbi The association between leukocytes and sperm quality is concentration dependent
    Jakob E Lackner
    Department of Urology, KH Hietzing mit Neurologischem Zentrum Rosenhügel and Ludwig Boltzmann Institut für Andrologie und Urologie, Vienna, Austria
    Reprod Biol Endocrinol 8:12. 2010
    ..To evaluate the association between leukocytes (polymorphonuclear granulocytes -PMNL) and semen parameters at different leukocyte concentrations...
  11. ncbi Relationship between oxidative stress, varicocele and infertility: a meta-analysis
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, USA
    Reprod Biomed Online 12:630-3. 2006
    ..56-216.96, P < 0.00001). From the results, it can be concluded that there is increased oxidative stress in varicocele patients; however, more studies are needed to confirm this finding...
  12. ncbi Role of free radicals in female reproductive diseases and assisted reproduction
    Ashok Agarwal
    Centre for Advanced Research in Human, Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH 44195, USA
    Reprod Biomed Online 9:338-47. 2004
    ..Research is in progress to identify the mechanisms that are involved in the aetiology of female reproductive diseases caused by ROS, and to create effective strategies that can counteract oxidative stress...
  13. ncbi Inter-sample variability in post-thaw human spermatozoa
    Kiran P Nallella
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Cryobiology 49:195-9. 2004
    ..Further research is necessary to understand the mechanism(s) responsible for this variability. This may also assist clinicians utilize semen samples with optimum semen quality in ART procedures...
  14. ncbi The effect of sperm DNA damage on assisted reproduction outcomes. A review
    A Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, OH 44195, USA
    Minerva Ginecol 56:235-45. 2004
    ..The results of this evaluation may be used to counsel the couple about their chances for live birth and for genomic abnormalities in their offspring...
  15. ncbi Oxidative stress in an assisted reproductive techniques setting
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 86:503-12. 2006
    ..The impact of oxidative stress on ART outcome and the different strategies designed to prevent it are also discussed...
  16. ncbi Role of antioxidants in treatment of male infertility: an overview of the literature
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Dept of Obstetrics and Gynecology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH, USA
    Reprod Biomed Online 8:616-27. 2004
    ..Multicentre, double-blind studies with statistically accepted sample size are still needed to provide conclusive evidence on the benefit of antioxidants as a treatment modality for patients with male infertility...
  17. ncbi Reactive oxygen species as an independent marker of male factor infertility
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 86:878-85. 2006
    ..To determine the abnormal patterns of reactive oxygen species (ROS) production in male factor infertility (MFI) patients and to define the ROS reference values in such patients...
  18. ncbi Carnitines and male infertility
    A Agarwal
    Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19 1, Cleveland, OH 44195, USA
    Reprod Biomed Online 8:376-84. 2004
    ..Additional, well-designed studies are necessary to further validate the use of carnitines in the treatment of patients with male infertility, specifically in men with poor semen quality...
  19. ncbi Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute, Cleveland, Ohio 44195, USA
    Fertil Steril 87:156-62. 2007
    ..To evaluate the performance of the automated semen quality analyzer system for assessing sperm quality...
  20. ncbi The role of free radicals and antioxidants in reproduction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and the Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Curr Opin Obstet Gynecol 18:325-32. 2006
    ..This review summarizes the role of free radicals and oxidative stress in the pathophysiology of human reproduction...
  21. ncbi Chemiluminescence technique for measuring reactive oxygen species
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Reprod Biomed Online 9:466-8. 2004
    ..A wide range of luminometers are available in the market, and laboratories should select the instruments that suit their individual needs...
  22. ncbi Role of oxidative stress in female reproduction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Reprod Biol Endocrinol 3:28. 2005
    ..Serial measurement of oxidative stress biomarkers in longitudinal studies may help delineate the etiology of some of the diosorders in female reproduction such as preeclampsia...
  23. ncbi Correlation of reactive oxygen species levels with the fertilization rate after in vitro fertilization: a qualified meta-analysis
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Fertil Steril 84:228-31. 2005
    ..We conclude that ROS has a statistically significant effect on the fertilization rate after IVF, and that the measurement of ROS levels in semen specimens before IVF may be useful in predicting the IVF outcome and in counseling patients...
  24. ncbi Prevention of oxidative stress injury to sperm
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Androl 26:654-60. 2005
  25. ncbi Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    BJU Int 95:503-7. 2005
  26. ncbi Implications of systemic malignancies on human fertility
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Reprod Biomed Online 9:673-9. 2004
    ..However, there are some ethical issues that should be considered before offering patients any of these options...
  27. ncbi Role of oxidative stress in the pathophysiological mechanism of erectile dysfunction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Ave, OH 44195, USA
    J Androl 27:335-47. 2006
  28. ncbi Sperm DNA damage assessment: a test whose time has come
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 84:850-3. 2005
    ..The available tests of sperm DNA damage require additional large-scale clinical trials before their integration into routine clinical practice...
  29. ncbi Oxidative stress and its implications in female infertility - a clinician's perspective
    Ashok Agarwal
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Department of Obstetrics Gynecology and Glickman Urological Institute, The Cleveland Clinic Foundation, OH 44195, USA
    Reprod Biomed Online 11:641-50. 2005
    ..This review discusses the sources of ROS in media used in IVF-embryo transfer and strategies to overcome OS in oocyte in-vitro maturation, in-vitro culture and sperm preparation techniques...
  30. ncbi Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 89:124-8. 2008
    ..To investigate the effect of cell phone use on various markers of semen quality...
  31. ncbi Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 89:375-9. 2008
    ..To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity...
  32. ncbi The role of antioxidant therapy in the treatment of male infertility
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Ob Gyn and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Hum Fertil (Camb) 13:217-25. 2010
    ....
  33. ncbi Potential biological role of poly (ADP-ribose) polymerase (PARP) in male gametes
    Ashok Agarwal
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA
    Reprod Biol Endocrinol 7:143. 2009
    ..PARP could provide new strategies to preserve fertility in cancer patients subjected to genotoxic stresses and may be a key to better male reproductive health...
  34. ncbi Vitrification of isolated mice blastomeres using a closed loading device
    Amr Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics and Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biol Endocrinol 7:17. 2009
    ..Using a cryotip and propagation in microvolumes, isolated mice blastomeres were vitrified and warmed with 100% post-warming survival...
  35. ncbi Role of oxidative stress in pathogenesis of varicocele and infertility
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obstetrics Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Urology 73:461-9. 2009
    ..We conclude that spermatozoal dysfunction in varicocele patients could be multifactorial, and oxidative stress-induced injury appears to be one of the main causes...
  36. ncbi What an andrologist/urologist should know about free radicals and why
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Cleveland, Ohio 44195, USA
    Urology 67:2-8. 2006
  37. ncbi Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: an in vitro pilot study
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 92:1318-25. 2009
    ..To evaluate effects of cellular phone radiofrequency electromagnetic waves (RF-EMW) during talk mode on unprocessed (neat) ejaculated human semen...
  38. ncbi Role of oxidants in male infertility: rationale, significance, and treatment
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Desk A 19 1, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Urol Clin North Am 29:817-27. 2002
    ..A double-blind, placebo-controlled study underway at the authors' center is evaluating the effects of treating infertile men with a combination of vitamins E and C after adjusting for all causes of controversy...
  39. ncbi New semen quality scores developed by principal component analysis of semen characteristics
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute and Department of Obstetrics Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Androl 24:343-52. 2003
    ..The composite scores can summarize overall SQ and quantity. Both SQ and RQ scores provide meaningful information on the quality of semen specimens for the clinician...
  40. ncbi Role of reactive oxygen species in the pathophysiology of human reproduction
    Ashok Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute and Department of Obstetrics Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 79:829-43. 2003
    ..To summarize the role of reactive oxygen species (ROS) in the pathophysiology of human reproduction...
  41. ncbi Lifestyle and testicular dysfunction: a brief update
    Ashok Agarwal
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obst Gyne and Women s Health Institute, Cleveland Clinic, Cleveland, OH, USA
    Biomed Pharmacother 62:550-3. 2008
    ....
  42. ncbi Efficacy of varicocelectomy in improving semen parameters: new meta-analytical approach
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological Institute, Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Urology 70:532-8. 2007
    ..To determine the efficacy of varicocelectomy in improving semen parameters...
  43. ncbi Emerging technologies for the molecular study of infertility, and potential clinical applications
    Alex C Varghese
    DeCode Life Foundation, Kolkata, India
    Reprod Biomed Online 15:451-6. 2007
    ..However, their widespread use in laboratories and clinics may not be seen until preliminary studies confirming their safety and effectiveness are published, and until standardized protocols for their utilization are established...
  44. ncbi Current and future perspectives on intracytoplasmic sperm injection: a critical commentary
    Alex C Varghese
    Molecular Research and Investigation Centre, Kolkata, India
    Reprod Biomed Online 15:719-27. 2007
    ..Since new studies on ICSI are published regularly, it is important that the established protocol is revised often, and that the role of ICSI in infertility therapy is continually re-evaluated...
  45. ncbi Clinical relevance of oxidative stress in male factor infertility: an update
    Ashok Agarwal
    Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, OH 44195, USA
    Am J Reprod Immunol 59:2-11. 2008
    ..The benefits of incorporating antioxidants in clinical and experimental settings have been enumerated. We also highlight the emerging concept of utilizing OS as a method of contraception and the potential problems associated with it...
  46. ncbi Redox considerations in female reproductive function and assisted reproduction: from molecular mechanisms to health implications
    Ashok Agarwal
    Reproductive Research Center, Department of Obstetrics and Gynecology, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Antioxid Redox Signal 10:1375-403. 2008
    ..Although antioxidants such as folate, zinc, and thiols may help enhance fertility, the available data are contentious and must be evaluated in controlled studies with larger populations...
  47. ncbi Role of sperm chromatin abnormalities and DNA damage in male infertility
    A Agarwal
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Hum Reprod Update 9:331-45. 2003
    ..Therefore, screening for sperm DNA damage may provide useful information in cases of male idiopathic infertility and in those men pursuing assisted reproduction. Treatment should include methods for prevention of sperm DNA damage...
  48. ncbi Assessing sperm function
    Ashok Agarwal
    Reproductive Research Center, Glickman Urological and Kidney Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Urol Clin North Am 35:157-71, vii. 2008
    ..Finally, promising new tests under development are mentioned with their potential clinical applications...
  49. ncbi Evaluation of nuclear DNA damage in spermatozoa from infertile men with varicocele
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 80:1431-6. 2003
    ..To examine levels of sperm DNA damage and oxidative stress (OS) in infertile men with varicocele...
  50. ncbi DNA damage in patients with untreated cancer as measured by the sperm chromatin structure assay
    H Kobayashi
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Fertil Steril 75:469-75. 2001
    ..CONCLUSION(S): DNA damage in spermatozoa is prevalent in the majority of cancer patients. SCSA provides important information about the biochemical integrity of sperm DNA in men with cancer before their treatment...
  51. ncbi Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility
    M Ollero
    Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Hum Reprod 16:1912-21. 2001
    ..DNA damage in mature spermatozoa may be the result of oxidative damage by ROS-producing immature spermatozoa during sperm migration from the seminiferous tubules to the epididymis...
  52. ncbi Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, The Cleveland Clinic Foundation, Ohio 44195, USA
    Fertil Steril 78:491-9. 2002
    ..Given the potential adverse effects of seminal OS on fertility, physicians should advise infertile men who smoke cigarettes to quit...
  53. ncbi Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation
    E Gil-Guzman
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, The Cleveland Clinic Foundation, Cleveland, OH 44165, USA
    Hum Reprod 16:1922-30. 2001
    ....
  54. ncbi Varicocelectomy improves intrauterine insemination success rates in men with varicocele
    J A Daitch
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, Department of Gynecology-Obstetrics and Biostatistics, Cleveland Clinic Foundation, Cleveland, Ohio, USA
    J Urol 165:1510-3. 2001
    ..A functional factor not measured on routine semen analysis may affect pregnancy rates in this setting. Men should be screened for varicocele before intrauterine insemination is initiated for male factor infertility...
  55. ncbi Oxidative stress in normospermic men undergoing infertility evaluation
    F F Pasqualotto
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute, Cleveland, Ohio, USA
    J Androl 22:316-22. 2001
    ..We conclude that oxidative stress is associated with male factor infertility. The presence of oxidative stress in infertile normospermic men may explain previously unexplained cases of infertility otherwise attributed to female factors...
  56. ncbi Relationship between seminal white blood cell counts and oxidative stress in men treated at an infertility clinic
    R K Sharma
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, Cleveland Clinic Foundation, Ohio 44195, USA
    J Androl 22:575-83. 2001
    ..Complete removal of WBCs from semen samples used for assisted reproduction may help reduce oxidative stress...
  57. ncbi A red palm oil diet can reduce the effects of oxidative stress on rat spermatozoa
    Y G Aboua
    Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, South Africa
    Andrologia 44:32-40. 2012
    ..We therefore propose that a daily intake of RPO supplement to the diet might be helpful in protecting males against the adverse effects of high ROS in sperm function and help preserve fertility...
  58. ncbi Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm characteristics
    A C Varghese
    Fertility Clinic and IVF Department, AMRI Medical Centre, Kolkata, India
    Andrologia 41:207-15. 2009
    ..Sperm assessment for DNA status using AO is reliable and shows good correlation with sperm count, motility and morphology. Assessment of sperm DNA status with AO staining may be helpful prior to ART...
  59. ncbi Evaluation of post-thaw DNA integrity of mouse blastocysts after ultrarapid and slow freezing
    Amr Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Fertil Steril 91:2087-94. 2009
    ..To evaluate the effect of vitrification and two other methods of slow cryopreservation on DNA integrity in expanded and nonexpanded blastocysts...
  60. ncbi Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study
    Xia Wang
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 80:844-50. 2003
    ..45). CONCLUSION(S): Measuring MMP in spermatozoa provides useful information about a man's fertility potential. Increased ROS production by spermatozoa is associated with a decreased MMP...
  61. ncbi Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay
    Juan G Alvarez
    Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    Fertil Steril 78:319-29. 2002
    ..CONCLUSION(S): The increase in chromatin alterations and DNA damage in sperm, as defined by the sperm chromatin structure assay from leukocytospermic samples may be related to alterations in the regulation of spermatogenesis...
  62. ncbi Differential growth of human embryos in vitro: role of reactive oxygen species
    Mohamed A Bedaiwy
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Fertil Steril 82:593-600. 2004
    ..Differential growth of ICSI embryos incubated under identical conditions may be in part due to differences in ROS levels of the culture medium surrounding these embryos...
  63. ncbi Oxidative stress & male infertility
    Kartikeya Makker
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Obsterics Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Indian J Med Res 129:357-67. 2009
    ..It also highlights the mechanism of production and the pathophysiology of ROS in relation to the male reproductive system and enumerate the benefits of incorporating antioxidants in clinical and experimental settings...
  64. ncbi Lipid peroxidation and antioxidant status in preeclampsia: a systematic review
    Sajal Gupta
    Glickman Urological and Kidney Institute, Cleveland, OH 44195, USA
    Obstet Gynecol Surv 64:750-9. 2009
    ..Preeclampsia is characterized by increased lipid peroxidation and diminished antioxidant capacity; however, there is no consensus as to the extent of these conditions...
  65. ncbi Protein supplementation and the incidence of apoptosis and oxidative stress in mouse embryos
    Navid Esfandiari
    Department of Obstetrics and Gynecology, Glickman Urological Institute, Cleveland, Ohio 44195, USA
    Obstet Gynecol 105:653-60. 2005
    ..68 and 5.81 +/- 1.11, respectively, P = .001). CONCLUSION: Protein supplementation of culture media improves the hatching rate and reduces reactive oxygen species levels and the incidence of apoptosis in mouse preimplantation embryos...
  66. ncbi Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis
    Marcello Cocuzza
    Reproductive Research Center, Glickman Urological and Kidney Institute, Department of Obstetrics Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Int Braz J Urol 33:603-21. 2007
    ..Thus evaluation of seminal ROS levels and extent of sperm DNA damage especially in an infertile male may help develop new therapeutic strategies and improve success of assisted reproductive techniques (ART)...
  67. ncbi Sperm DNA damage and its clinical relevance in assessing reproductive outcome
    R K Sharma
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Desk A19.1, Cleveland, OH 44195, USA
    Asian J Androl 6:139-48. 2004
    ..In turn, this may alleviate the financial, social and emotional problems associated with failed ART attempts...
  68. ncbi Free radicals: their beneficial and detrimental effects on sperm function
    Shiva Kothari
    Center for Reproductive Medicine, Cleveland Clinic, 9500 Euclid Avenue, Desk A19 1, Cleveland, Ohio 44195, USA
    Indian J Exp Biol 48:425-35. 2010
    ..Pathologically, free radicals induce lipid peroxidation, DNA damage and apoptosis of spermatozoa. The present review deals with both the beneficial and detrimental effects of free radicals on sperm function...
  69. ncbi Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility
    Xia Wang
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Department of Obsterics and Gynecology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 80:531-5. 2003
    ..A positive relationship exists between increased sperm damage by ROS and higher levels of cytochrome c and caspases 9 and 3, which indicate positive apoptosis in patients with male factor infertility...
  70. ncbi Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 79:1597-605. 2003
    ..Such an increase may be related to high levels of seminal oxidative stress...
  71. ncbi Technical and ethical challenges of fertility preservation in young cancer patients
    Fnu Deepinder
    Reproductive Research Center, Glickman Urological and Kidney Institute and Department of Obstetrics Gynecology, The Cleveland Clinic, Cleveland, OH, USA
    Reprod Biomed Online 16:784-91. 2008
    ..This review summarizes the technical and ethical challenges of gamete cryopreservation in young cancer patients...
  72. ncbi Poor semen quality and ROS-TAC scores in patients with idiopathic infertility
    Fabio F Pasqualotto
    Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, USA
    Urol Int 81:263-70. 2008
    ....
  73. ncbi Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species
    Reda Mahfouz
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 94:2141-6. 2010
    ..To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS)...
  74. ncbi Present and future fertility preservation strategies for female cancer patients
    Elena S Georgescu
    Center for Reproductive Medicine, Cleveland Clinic, Ohio 44195, USA
    Obstet Gynecol Surv 63:725-32. 2008
    ....
  75. ncbi Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa
    Ramadan A Saleh
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 78:1215-24. 2002
    ..Such stimulation may be mediated via direct cell-cell contact or by soluble products released by leukocytes. Poor sperm quality in leukocytospermic samples may be due to leukocyte-mediated oxidative stress...
  76. ncbi Male gamete survival at stake: causes and solutions
    Alex C Varghese
    Reproductive Research Centre, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biomed Online 17:866-80. 2008
    ..This review aims to include all the possible causes of disruption to this unique cell and their probable solutions, in the hope of clearing up the ambiguity that surrounds male factor infertility...
  77. ncbi Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility
    Mohamed H Moustafa
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Hum Reprod 19:129-38. 2004
    ..57 (0.18-0.97)]. CONCLUSIONS: DNA damage may be induced by oxidative assault. Apoptosis may not contribute significantly to the DNA damage...
  78. ncbi Identification of male factor infertility using a novel semen quality score and reactive oxygen species levels
    Kiran P Nallella
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute, Cleveland, Ohio, USA
    Clinics 60:317-24. 2005
    ..Because both of these parameters are more sensitive than individual sperm parameters in identifying male factor infertility, they should be included in routine infertility screening...
  79. ncbi Relationship of interleukin-6 with semen characteristics and oxidative stress in patients with varicocele
    Kiran P Nallella
    Center for Advanced Research in Human Reproduction, Infertility and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Urology 64:1010-3. 2004
    ..Pro-inflammatory cytokine IL-6 and oxidative stress may play a role in the pathophysiology of infertility in these patients...
  80. ncbi Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures
    Eleonora B Pasqualotto
    Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 81:973-6. 2004
    ..CONCLUSION(S): Both LPO and TAC levels were positively correlated with the pregnancy rate. Lipid peroxidation may be a good marker of metabolic activity within the follicle, and some amounts may be necessary to establish a pregnancy...
  81. ncbi Human sperm superoxide anion generation and correlation with semen quality in patients with male infertility
    Tamer M Said
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, The Cleveland Clinic Foundation, OH 44195, USA
    Fertil Steril 82:871-7. 2004
    ..36-1). CONCLUSION(S): Spermatozoa from infertile men produce higher levels of O(2)(.-) in the presence of exogenous NADPH compared to healthy donors. The ability of spermatozoa to generate O(2)(.-) increases as the semen quality declines...
  82. ncbi Role of total antioxidant capacity in the differential growth of human embryos in vitro
    Mohamed Bedaiwy
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Fertil Steril 86:304-9. 2006
    ..Decreased embryonic fragmentation, enhanced cleavage rate, and increased blastocyst development rate may be partially related to day 1 TAC in the culture media. Whether this relationship is a cause or effect needs further assessment...
  83. ncbi Role of oxidative stress in endometriosis
    Sajal Gupta
    Centre for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics-Gynecology, The Cleveland Clinic Foundation, OH, USA
    Reprod Biomed Online 13:126-34. 2006
    ..Results of the limited number of animal and human trials need to be corroborated by larger randomized controlled trials...
  84. ncbi p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts
    M L Agarwal
    Department of Molecular Biology, Cleveland Clinic Foundation, OH 44195, USA
    Proc Natl Acad Sci U S A 92:8493-7. 1995
    ..Therefore, irreversible arrest must involve processes other than or in addition to the interaction of p53-induced p21/WAF1 with G1 and G2 cyclin-dependent kinases...
  85. ncbi Serum and peritoneal abnormalities in endometriosis: potential use as diagnostic markers
    S Gupta
    Reproductive Research Center, Glickman Urological Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Minerva Ginecol 58:527-51. 2006
    ..Potential new therapeutic agents include modulators, such as cytokine receptor blockers and angiogenic receptor blockers, presently used for treating endometriosis...
  86. ncbi Pathophysiology of varicoceles in male infertility
    C K Naughton
    Center for Advanced Research in Human Reproduction and Infertility, Department of Urology, The Cleveland Clinic Foundation, Ohio 44195, USA
    Hum Reprod Update 7:473-81. 2001
    ....
  87. ncbi Regulation of p53 expression by the RAS-MAP kinase pathway
    M L Agarwal
    Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio, OH 44195, USA
    Oncogene 20:2527-36. 2001
    ..We conclude that MAP kinase-dependent pathways help to regulate p53 levels by regulating the expression of p53 mRNA...
  88. ncbi Relationship of interleukin-6 with semen characteristics and oxidative stress in vasectomy reversal patients
    K C Nandipati
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Glickman Urological Institute and Department of Obstetrics and Gynecology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Andrologia 37:131-4. 2005
    ..41, P = 0.05 versus r = 0.38, P = 0.15). We conclude that patients who undergo vasectomy reversal have elevated levels of IL-6 and ROS, which may contribute to decreased sperm motility and concentration and possibly lead to sub-fertility...
  89. ncbi Relationship of reactive oxygen species levels in day 3 culture media to the outcome of in vitro fertilization/intracytoplasmic sperm injection cycles
    Mohamed A Bedaiwy
    Department of Obstetrics and Gynecology, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio 44195, USA
    Fertil Steril 94:2037-42. 2010
    ..To examine the relationship of early human embryonic development parameters to day 3 reactive oxygen species (D-3 ROS) levels in culture media...
  90. ncbi Reactive oxygen species levels are independent of sperm concentration, motility, and abstinence in a normal, healthy, proven fertile man: a longitudinal study
    Nisarg R Desai
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 94:1541-3. 2010
    ..We suggest that fluctuation in seminal ROS values may be related to physiologic or transient changes in spermatogenesis...
  91. ncbi Effect of varying equilibration time in a two-step vitrification method on the post-warming DNA integrity of mouse blastocysts
    Amr Kader
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, and Obstetrics and Gynecology and Women s Health Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 93:2640-5. 2010
    ..To assess the effect of equilibration time on the DNA integrity of vitrified-warmed mouse blastocysts...
  92. ncbi Current trends, biological foundations and future prospects of oocyte and embryo cryopreservation
    Alex C Varghese
    Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA
    Reprod Biomed Online 19:126-40. 2009
    ..The transcriptomics, proteomics and metabolomic platforms hold promise for elucidating these complex processes during cryopreservation processes...
  93. ncbi Evaluation of poly(ADP-ribose) polymerase cleavage (cPARP) in ejaculated human sperm fractions after induction of apoptosis
    Reda Z Mahfouz
    Reproductive Research Center, Glickman Urological and Kidney Institute, Department of Obstetrics and Gynecology, Cleveland Clinic, Cleveland, Ohio 44195, USA
    Fertil Steril 91:2210-20. 2009
    ..To examine the presence of cleaved poly(ADP-ribose) polymerase(s) (cPARP) in ejaculated spermatozoa and determine cPARP levels following exposure to chemical or oxidative stress...
  94. ncbi The impact of peritoneal fluid from healthy women and from women with endometriosis on sperm DNA and its relationship to the sperm deformity index
    Gihan Mansour
    Obstetrics and Gynecology and Women s Health Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Fertil Steril 92:61-7. 2009
    ..To study the effect of peritoneal fluid (PF) from healthy women and from women with endometriosis on sperm DNA and its relationship to sperm morphology as assessed by the sperm deformity index (SDI)...
  95. ncbi New generation of diagnostic tests for infertility: review of specialized semen tests
    Mary K Samplaski
    Glickman Urological and Kidney Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Int J Urol 17:839-47. 2010
    ..This article reviews basic male reproductive physiology, as well as clinically utilized advanced seminal tests, including their methodology and implications for reproductive technology outcomes and fecundity...
  96. ncbi Vitamin C and vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocyst development rate
    Xia Wang
    Department of Obstetrics and Gynecology, Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, Cleveland, Ohio 44195, USA
    Fertil Steril 78:1272-7. 2002
    ..Exposure of mouse embryos to ROS for extended periods results in embryotoxicity. Vitamin C is more effective than vitamin E in reversing ROS-induced mouse embryo toxicity...
  97. ncbi Human sperm DNA oxidation, motility and viability in the presence of L-carnitine during in vitro incubation and centrifugation
    S Banihani
    Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    Andrologia 44:505-12. 2012
    ..In conclusion, LC may enhance sperm motility following incubation and centrifugation, while it might not affect sperm viability and DNA oxidation...
  98. ncbi Superovulation and intrauterine insemination in cases of treated mild pelvic disease
    M Singh
    Center for Advanced Research in Human Reproduction and Infertility, Urological Institute and Department of Gynecology-Obstetrics, Cleveland Clinic Foundation, 9500 Euclid Avenue, A81, Cleveland, Ohio 44195, USA
    J Assist Reprod Genet 18:26-9. 2001
    ..However, SO/IUI still remains a reasonable option for both these groups prior to IVF-ET...
  99. ncbi Prediction of endometriosis with serum and peritoneal fluid markers: a prospective controlled trial
    M A Bedaiwy
    Center for Advanced Research in Human Reproduction, Infertility, and Sexual Function, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Hum Reprod 17:426-31. 2002
    ..Before these markers can be used as a non-surgical diagnostic tool, these data should be verified in a larger study...
  100. ncbi Early combination therapy: intracavernosal injections and sildenafil following radical prostatectomy increases sexual activity and the return of natural erections
    K Nandipati
    Glickman Urological Institute, Cleveland Clinic Foundation, Cleveland, OH 44125, USA
    Int J Impot Res 18:446-51. 2006
    ..Early combination therapy with sildenafil allowed a lower dose of intracavernous injections, minimizing the penile discomfort...
  101. ncbi Management of erectile dysfunction following radical prostatectomy
    C D Zippe
    Andrology Urology Research Laboratory, Urological Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, A100, Cleveland, OH 44195, USA
    Curr Urol Rep 2:495-503. 2001
    ..The potential impact of sildenafil and other new oral therapies should encourage urologists to continue to perform and perfect the nerve-sparing approach...