Rafat Siddiqui

Summary

Affiliation: Clarian Health Partners
Country: USA

Publications

  1. pmc A synergistic antiproliferation effect of curcumin and docosahexaenoic acid in SK-BR-3 breast cancer cells: unique signaling not explained by the effects of either compound alone
    Jeffrey D Altenburg
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, Indiana, USA
    BMC Cancer 11:149. 2011
  2. ncbi request reprint Modulation of lipid rafts by Omega-3 fatty acids in inflammation and cancer: implications for use of lipids during nutrition support
    Rafat A Siddiqui
    Methodist Research Institute, Cellular Biochemistry, Indianapolis, IN 46202, USA
    Nutr Clin Pract 22:74-88. 2007
  3. ncbi request reprint Modulation of enzymatic activities by n-3 polyunsaturated fatty acids to support cardiovascular health
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health, Indianapolis, IN 46202, USA
    J Nutr Biochem 19:417-37. 2008
  4. pmc Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN, USA
    Breast Cancer Res 7:R645-54. 2005
  5. doi request reprint Anticancer properties of oxidation products of docosahexaenoic acid
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, United States
    Chem Phys Lipids 153:47-56. 2008
  6. ncbi request reprint Omega 3-fatty acids: health benefits and cellular mechanisms of action
    Rafat A Siddiqui
    Methodist Research Institute, Indianapolis, Indiana 46202, USA
    Mini Rev Med Chem 4:859-71. 2004
  7. doi request reprint Attenuation of proteolysis and muscle wasting by curcumin c3 complex in MAC16 colon tumour-bearing mice
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, USA
    Br J Nutr 102:967-75. 2009
  8. ncbi request reprint Inhibition of phenylephrine-induced cardiac hypertrophy by docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Blvd, Indianapolis, Indiana 46202, USA
    J Cell Biochem 92:1141-59. 2004
  9. doi request reprint n-3 fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death
    Rafat A Siddiqui
    Cellular Biochemistry and Lipid Biology Laboratories, Methodist Research Institute, Indianapolis, IN, USA
    Br J Nutr 102:1811-9. 2009
  10. pmc Cell-cycle arrest in Jurkat leukaemic cells: a possible role for docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Boulevard, Indianapolis, IN 46202, USA
    Biochem J 371:621-9. 2003

Research Grants

  1. Angiogenesis in the Pulmonary Microvasculature
    Rafat Siddiqui; Fiscal Year: 2004

Collaborators

Detail Information

Publications29

  1. pmc A synergistic antiproliferation effect of curcumin and docosahexaenoic acid in SK-BR-3 breast cancer cells: unique signaling not explained by the effects of either compound alone
    Jeffrey D Altenburg
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, Indiana, USA
    BMC Cancer 11:149. 2011
    ....
  2. ncbi request reprint Modulation of lipid rafts by Omega-3 fatty acids in inflammation and cancer: implications for use of lipids during nutrition support
    Rafat A Siddiqui
    Methodist Research Institute, Cellular Biochemistry, Indianapolis, IN 46202, USA
    Nutr Clin Pract 22:74-88. 2007
    ..We review how alteration of raft lipids affects the raft/nonraft localization and hence the function of several proteins involved in cell signaling. We focus our discussion of raft-signaling proteins on inflammation and cancer...
  3. ncbi request reprint Modulation of enzymatic activities by n-3 polyunsaturated fatty acids to support cardiovascular health
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health, Indianapolis, IN 46202, USA
    J Nutr Biochem 19:417-37. 2008
    ..This review is therefore focused on the molecular targets and signaling pathways that are regulated by n-3 PUFAs in relation to their cardioprotective effects...
  4. pmc Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN, USA
    Breast Cancer Res 7:R645-54. 2005
    ..The present study describes the synthesis, purification, characterization and evaluation of two novel anticancer conjugates, propofol-docosahexaenoate (propofol-DHA) and propofol-eicosapentaenoate (propofol-EPA)...
  5. doi request reprint Anticancer properties of oxidation products of docosahexaenoic acid
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, United States
    Chem Phys Lipids 153:47-56. 2008
    ..We conclude that the study of the effects of lipid peroxidation products on biochemical function will be a difficult but highly rewarding area for future studies...
  6. ncbi request reprint Omega 3-fatty acids: health benefits and cellular mechanisms of action
    Rafat A Siddiqui
    Methodist Research Institute, Indianapolis, Indiana 46202, USA
    Mini Rev Med Chem 4:859-71. 2004
    ..Protein phosphatases are also involved with the protein Bcl2, which regulates the release of cytochrome c from mitochondria, and eventually, activation of the apoptotic enzyme caspase 3...
  7. doi request reprint Attenuation of proteolysis and muscle wasting by curcumin c3 complex in MAC16 colon tumour-bearing mice
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, USA
    Br J Nutr 102:967-75. 2009
    ..In conclusion, oral curcumin c3 results in the prevention and reversal of weight loss. The data imply that curcumin c3 may be an effective adjuvant therapy against cachexia...
  8. ncbi request reprint Inhibition of phenylephrine-induced cardiac hypertrophy by docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Blvd, Indianapolis, Indiana 46202, USA
    J Cell Biochem 92:1141-59. 2004
    ..In vivo studies are needed to confirm these in vitro effects of DHA...
  9. doi request reprint n-3 fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death
    Rafat A Siddiqui
    Cellular Biochemistry and Lipid Biology Laboratories, Methodist Research Institute, Indianapolis, IN, USA
    Br J Nutr 102:1811-9. 2009
    ..The in vitro study demonstrates that TFA induce, while n-3 PUFA prevent, vascular inflammation...
  10. pmc Cell-cycle arrest in Jurkat leukaemic cells: a possible role for docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Boulevard, Indianapolis, IN 46202, USA
    Biochem J 371:621-9. 2003
    ..The net effect of cdk2 inhibition and protein phosphatase activation is an inhibition of pRb phosphorylation, consequently arresting Jurkat cell growth...
  11. ncbi request reprint Prevention of docosahexaenoic acid-induced cytotoxicity by phosphatidic acid in Jurkat leukemic cells: the role of protein phosphatase-1
    R A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute at Clarian Health, Indianapolis, IN 46202, USA
    Biochim Biophys Acta 1541:188-200. 2001
    ..Since DHA-induced apoptosis can be inhibited by PA, we conclude that the process is mediated through activation of PP1...
  12. ncbi request reprint (N-3) long-chain polyunsaturated fatty acids prolong survival following myocardial infarction in rats
    Gary P Zaloga
    Department of Biology, Indiana University Purdue University, Indianapolis, IN, USA
    J Nutr 136:1874-8. 2006
    ..Based upon our results, we speculate that decreased activities of protein kinases induced by diets high in (n-3) PUFAs are associated with a decrease in sudden death after MI in rats...
  13. ncbi request reprint Green tea polyphenols modulate secretion of urokinase plasminogen activator (uPA) and inhibit invasive behavior of breast cancer cells
    Veronika Slivova
    Cancer Research Laboratory, Methodist Research Institute, Indianapolis, IN 46202, USA
    Nutr Cancer 52:66-73. 2005
    ..Our results indicate that GTP may contribute to the anticancer effects of green tea by inhibiting the invasive behavior of cancer cells...
  14. ncbi request reprint Docosahexaenoic acid induces apoptosis in Jurkat cells by a protein phosphatase-mediated process
    R A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute at Clarian Health, Indianapolis, IN 46202, USA
    Biochim Biophys Acta 1499:265-75. 2001
    ..These results suggest that one mechanism through which DHA may control cancer cell growth is through apoptosis involving PP1/PP2B protein phosphatase activities...
  15. ncbi request reprint Nutrition modulation of cachexia/proteolysis
    Rafat Siddiqui
    Methodist Research Institute, 1812 N Capitol Ave, Wile Hall, Room 120, Indianapolis, IN 46202, USA
    Nutr Clin Pract 21:155-67. 2006
    ..Optimal treatment for cachexia is likely aimed at maximizing muscle and adipose synthesis while minimizing degradation...
  16. ncbi request reprint Docosahexaenoic acid affects cell signaling by altering lipid rafts
    William Stillwell
    Department of Biology, Indiana University, Purdue University Indianapolis, 723 West Michigan, Indianapolis, IN 46202, USA
    Reprod Nutr Dev 45:559-79. 2005
    ..We conclude with a proposal of how DHA incorporation into membranes may control cell biochemistry and physiology...
  17. doi request reprint An improved method for determining medium- and long-chain FAMEs using gas chromatography
    Zhidong Xu
    Methodist Research Institute, Clarian Health, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Lipids 45:199-208. 2010
    ..25 mm ID, 0.25 microm). Our data suggest that the improved method can be easily used to accurately determine fatty acids (C6-C24) in functional foods and lipid emulsions...
  18. ncbi request reprint A soluble ATP-dependent proteolytic system is responsible for protein degradation
    Gary P Zaloga
    Methodist Research Institute, 1812 N Capitol Ave, Wile Hall, Room 120, Indianapolis, IN 26202, USA
    Nutr Clin Pract 21:88-91. 2006
  19. pmc Acute rise of omega-3 polyunsaturated fatty acids during hemodialysis treatment
    Allon N Friedman
    Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Ren Nutr 18:301-3. 2008
    ..We performed this study to measure the effect of a single-standard hemodialysis treatment on plasma and erythrocyte omega-3 PUFA levels in chronic hemodialysis patients...
  20. ncbi request reprint Phosphatidylinositol 3'-kinase-mediated calcium mobilization regulates chemotaxis in phosphatidic acid-stimulated human neutrophils
    R A Siddiqui
    Experimental Cell Research Program, The Methodist Research Institute, MPC 1417, 1701 N Senate Ave, Indianapolis, IN 46202, USA
    Biochim Biophys Acta 1483:161-73. 2000
    ..We, therefore, propose that products of PI 3'-kinase confined to the inner leaflet of the plasma membrane play a role in activation of syk, calcium mobilization and induction of chemotactic migration...
  21. ncbi request reprint Phosphatidic acid induces calcium influx in neutrophils via verapamil-sensitive calcium channels
    R A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis, Indiana
    J Cell Biochem 78:297-304. 2000
    ..The results of this study suggest that exogenously added PA stimulates the generation of intracellular PA, which then mediates Ca(2+) influx through verapamil-sensitive Ca(2+) channels...
  22. ncbi request reprint Trans-fatty acids induce pro-inflammatory responses and endothelial cell dysfunction
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health, 1701 N Senate Room E504, Indianapolis, IN 46202, USA
    Br J Nutr 99:723-31. 2008
    ..In conclusion, these in vitro studies demonstrated that TFA play a role in the induction of pro-inflammatory responses and endothelial cell dysfunction...
  23. ncbi request reprint Omega-3 polyunsaturated fatty acids attenuate breast cancer growth through activation of a neutral sphingomyelinase-mediated pathway
    Min Wu
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, USA
    Int J Cancer 117:340-8. 2005
    ..05). The results suggest that inhibition of breast cancer growth in nude mice by dietary fish oil and inhibition of breast cancer cell growth in culture by treatment with DHA and EPA is mediated by activation of N-SMYase...
  24. ncbi request reprint Endothelial cell fatty acid unsaturation mediates cold-induced oxidative stress
    Michael A J Zieger
    Methodist Research Institute, Clarian Health Partners, Inc, Indianapolis, Indiana 46202, USA
    J Cell Biochem 99:784-96. 2006
    ..In conclusion, the findings implicate iron catalyzed free radicals and LPO as a predominant mechanism of endothelial cell injury at 0 degrees C, which may be reduced by increasing lipid saturation or treating cells with antioxidants...
  25. ncbi request reprint Biologically active dietary peptides
    Gary P Zaloga
    Methodist Research Institute of Clarian Health Inc and Department of Medicine of Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Mini Rev Med Chem 4:815-21. 2004
    ..We also review biologic effects of different protein sources, which generate different peptide profiles during digestion. The implications of dietary peptides for modulation of disease are discussed...
  26. pmc Oleic and docosahexaenoic acid differentially phase separate from lipid raft molecules: a comparative NMR, DSC, AFM, and detergent extraction study
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University, Indianapolis, Indiana 46202 5132, USA
    Biophys J 87:1752-66. 2004
    ..We conclude that the OA-PE phase separates from rafts far less than DHA-PE, which may have implications for cellular signaling...
  27. ncbi request reprint Acyl chain unsaturation in PEs modulates phase separation from lipid raft molecules
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Biochem Biophys Res Commun 311:793-6. 2003
    ..Our findings demonstrate that increasing unsaturation in PEs results in increased phase separation from SM/CHOL membranes, which may have implications for cellular signaling...
  28. ncbi request reprint Diverse signaling pathways regulate fibroblast differentiation and transformation through Rho kinase activation
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Indianapolis, Indiana, USA
    J Cell Physiol 211:353-63. 2007
    ....

Research Grants1

  1. Angiogenesis in the Pulmonary Microvasculature
    Rafat Siddiqui; Fiscal Year: 2004
    ..abstract_text> ..