Li Zheng

Summary

Affiliation: City of Hope National Medical Center
Country: USA

Publications

  1. ncbi request reprint Complementary functions of the Saccharomyces cerevisiae Rad2 family nucleases in Okazaki fragment maturation, mutation avoidance, and chromosome stability
    Xuemin Sun
    Division of Molecular Biology, City of Hope National Medical Center, Duarte, CA 91010, USA
    DNA Repair (Amst) 2:925-40. 2003
  2. pmc Polyploid cells rewire DNA damage response networks to overcome replication stress-induced barriers for tumour progression
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, California 91010, USA
    Nat Commun 3:815. 2012
  3. pmc Fen1 mutations that specifically disrupt its interaction with PCNA cause aneuploidy-associated cancer
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Cell Res 21:1052-67. 2011
  4. pmc Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding
    Zhigang Guo
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California, USA
    Nat Chem Biol 6:766-73. 2010
  5. pmc The DNA-protein interaction modes of FEN-1 with gap substrates and their implication in preventing duplication mutations
    Ren Liu
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 34:1772-84. 2006
  6. pmc Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, CA 91010, USA
    Mol Cell Biol 28:4310-9. 2008
  7. pmc Human DNA2 is a mitochondrial nuclease/helicase for efficient processing of DNA replication and repair intermediates
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Mol Cell 32:325-36. 2008
  8. ncbi request reprint Interaction and stimulation of human FEN-1 nuclease activities by heterogeneous nuclear ribonucleoprotein A1 in alpha-segment processing during Okazaki fragment maturation
    Qing Chai
    Division of Molecular Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Biochemistry 42:15045-52. 2003
  9. ncbi request reprint Concerted action of exonuclease and Gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG)n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments
    Purnima Singh
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    J Biol Chem 282:3465-77. 2007
  10. ncbi request reprint Fen1 mutations result in autoimmunity, chronic inflammation and cancers
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, California 91010, USA
    Nat Med 13:812-9. 2007

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Complementary functions of the Saccharomyces cerevisiae Rad2 family nucleases in Okazaki fragment maturation, mutation avoidance, and chromosome stability
    Xuemin Sun
    Division of Molecular Biology, City of Hope National Medical Center, Duarte, CA 91010, USA
    DNA Repair (Amst) 2:925-40. 2003
    ..Furthermore, Rad2 and Exo1 complemented the mutator phenotypes and cell cycle defects of rad27 mutant strains to differing extents, suggesting distinct in vivo nucleic acid substrates...
  2. pmc Polyploid cells rewire DNA damage response networks to overcome replication stress-induced barriers for tumour progression
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, California 91010, USA
    Nat Commun 3:815. 2012
    ..These molecular changes rewire DNA damage response and repair gene networks in polyploid tumour cells, enabling them to escape replication stress-induced senescence barriers...
  3. pmc Fen1 mutations that specifically disrupt its interaction with PCNA cause aneuploidy-associated cancer
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Cell Res 21:1052-67. 2011
    ..Thus, this study establishes an exemplary case for investigating the biological significance of protein-protein interactions by knock-in of a point mutation rather than knock-out of a whole gene...
  4. pmc Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding
    Zhigang Guo
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California, USA
    Nat Chem Biol 6:766-73. 2010
    ..This consequently leads to a defect in Okazaki fragment maturation, a delay in cell cycle progression, impairment of DNA repair and a high frequency of genome-wide mutations...
  5. pmc The DNA-protein interaction modes of FEN-1 with gap substrates and their implication in preventing duplication mutations
    Ren Liu
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 34:1772-84. 2006
    ..Furthermore, based on the evidence that GEN activity in complex with WRN protein cleaves hairpin and internal loop substrates, we suggest that the GEN activity may prevent repeat expansions and duplication mutations...
  6. pmc Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, CA 91010, USA
    Mol Cell Biol 28:4310-9. 2008
    ..The replacement of Ser(187) by Ala, eliminating the only phosphorylation site, retains FEN1 in nucleoli. Both of the mutations cause UV sensitivity, impair cellular UV damage repair capacity, and decline overall cellular survivorship...
  7. pmc Human DNA2 is a mitochondrial nuclease/helicase for efficient processing of DNA replication and repair intermediates
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Mol Cell 32:325-36. 2008
    ..Taken together, our studies illustrate an evolutionarily diversified role of hDNA2 in mitochondrial DNA replication and repair in a mammalian system...
  8. ncbi request reprint Interaction and stimulation of human FEN-1 nuclease activities by heterogeneous nuclear ribonucleoprotein A1 in alpha-segment processing during Okazaki fragment maturation
    Qing Chai
    Division of Molecular Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Biochemistry 42:15045-52. 2003
    ..With longer flap substrates the hnRNP A1 effect is more modest and is suppressed by the addition of RPA. A model is provided to explain the possible in vivo role of this interaction and activity in Okazaki fragment maturation...
  9. ncbi request reprint Concerted action of exonuclease and Gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG)n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments
    Purnima Singh
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    J Biol Chem 282:3465-77. 2007
    ..A model is presented to explain how the concerted action of EXO and GEN activities may contribute to resolving structured flaps, thereby preventing their expansion in the genome...
  10. ncbi request reprint Fen1 mutations result in autoimmunity, chronic inflammation and cancers
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, California 91010, USA
    Nat Med 13:812-9. 2007
    ....
  11. pmc Novel function of the flap endonuclease 1 complex in processing stalled DNA replication forks
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    EMBO Rep 6:83-9. 2005
    ..Replication protein A facilitates FEN-1 interaction with DNA bubble structures. Human FEN-1, but not the GEN-deficient mutant, E178A, was shown to rescue the defect in resistance to UV and camptothecin in a yeast FEN-1 null mutant...
  12. pmc Disruption of the FEN-1/PCNA interaction results in DNA replication defects, pulmonary hypoplasia, pancytopenia, and newborn lethality in mice
    Li Zheng
    City of Hope National Medical Center, Department of Molecular Biology, 1500 East Duarte Rd, Duarte, CA 91010, USA
    Mol Cell Biol 27:3176-86. 2007
    ..All newborn FFAA mutant pups died at birth, likely due to pulmonary hypoplasia and pancytopenia. Collectively, our data demonstrate the importance of the FEN-1/PCNA complex in DNA replication and in the embryonic development of mice...
  13. pmc Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity
    Weiqiang Lin
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    EMBO J 32:1425-39. 2013
    ....
  14. pmc High risk of benzo[α]pyrene-induced lung cancer in E160D FEN1 mutant mice
    Zhenxing Wu
    Colleges of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    Mutat Res 731:85-91. 2012
    ..All together, our current study suggests that individuals carrying the GEN-deficient FEN1 mutations have high risk to develop lung cancer upon exposure to B[α]P-containing agents such as tobacco smoke...
  15. ncbi request reprint Multiple but dissectible functions of FEN-1 nucleases in nucleic acid processing, genome stability and diseases
    Binghui Shen
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Bioessays 27:717-29. 2005
    ..This review summarizes the knowledge gained through efforts in the past decade to define its structural elements for specific activities and possible pathological consequences of altered functions of this multirole player...
  16. pmc Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center, Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 37:3431-41. 2009
    ..These data indicate that polymorphisms in base excision repair genes may contribute to the onset and development of cancers...
  17. pmc Sequential posttranslational modifications program FEN1 degradation during cell-cycle progression
    Zhigang Guo
    Jiangsu Key Laboratory for Molecular and Medical Biotechnology and College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
    Mol Cell 47:444-56. 2012
    ..These findings may represent a newly identified regulatory mechanism used by cells to ensure precise cell-cycle progression and to prevent transformation...
  18. pmc Okazaki fragment maturation: nucleases take centre stage
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Mol Cell Biol 3:23-30. 2011
    ..On the other hand, mutations that impair editing out of polymerase α incorporation errors result in cancer displaying a strong mutator phenotype...
  19. ncbi request reprint Suppression of Saccharomyces cerevisiae rad27 null mutant phenotypes by the 5' nuclease domain of Escherichia coli DNA polymerase I
    Xuemin Sun
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Curr Genet 41:379-88. 2002
    ..We conclude that the N-terminal nuclease domain of E. coli polymerase I encodes a functional homologue of FEN-1...
  20. ncbi request reprint Functional alterations of human exonuclease 1 mutants identified in atypical hereditary nonpolyposis colorectal cancer syndrome
    Xuemin Sun
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Cancer Res 62:6026-30. 2002
    ..The combination of these three point mutations leads to the binding capacity with hMsh2 to nearly zero. Evidence made available in this study sheds light on the pathogenesis of HNPCC, perhaps initiated by an additional MMR gene, hEXO1...
  21. ncbi request reprint Human 3-methyladenine-DNA glycosylase: effect of sequence context on excision, association with PCNA, and stimulation by AP endonuclease
    Liqun Xia
    Biology Division, Beckman Research Institute, City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    J Mol Biol 346:1259-74. 2005
    ..Thus, PCNA could have a role in short-patch BER as well as in long-patch BER. Overall, the data reported here show how multiple factors contribute to the activity of MPG in cells...
  22. ncbi request reprint Distinct roles of two Mg2+ binding sites in regulation of murine flap endonuclease-1 activities
    Li Zheng
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Biochemistry 41:10323-31. 2002
    ..Taken together, our results suggest distinct roles for two Mg(2+) binding sites in the regulation of mFEN-1 nuclease activities in a mode different from the "two-metal mechanism"...