P J Yazaki

Summary

Affiliation: City of Hope National Medical Center
Country: USA

Publications

  1. pmc A series of anti-CEA/anti-DOTA bispecific antibody formats evaluated for pre-targeting: comparison of tumor uptake and blood clearance
    Paul J Yazaki
    Department of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Protein Eng Des Sel 26:187-93. 2013
  2. pmc Biodistribution and tumor imaging of an anti-CEA single-chain antibody-albumin fusion protein
    Paul J Yazaki
    Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Nucl Med Biol 35:151-8. 2008
  3. ncbi request reprint Humanization of the anti-CEA T84.66 antibody based on crystal structure data
    Paul J Yazaki
    Department of Radioimmunotherapy, Division of Radiology, City of Hope National Medical Center, 1500 East Duarte Road, Duarte, CA 91010, USA
    Protein Eng Des Sel 17:481-9. 2004
  4. ncbi request reprint Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical applications
    P J Yazaki
    Department of Molecular Biology, Beckman Research Institute of the City of Hope, 91010, Duarte, CA, USA
    J Immunol Methods 253:195-208. 2001
  5. ncbi request reprint Tumor targeting of radiometal labeled anti-CEA recombinant T84.66 diabody and t84.66 minibody: comparison to radioiodinated fragments
    P J Yazaki
    Department of Molecular Biology and Division of Immunology of the Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Bioconjug Chem 12:220-8. 2001
  6. ncbi request reprint Numerical selection of optimal tumor imaging agents with application to engineered antibodies
    L E Williams
    Division of Radiology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA, USA
    Cancer Biother Radiopharm 16:25-35. 2001
  7. pmc High-resolution microPET imaging of carcinoembryonic antigen-positive xenografts by using a copper-64-labeled engineered antibody fragment
    A M Wu
    Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 97:8495-500. 2000
  8. ncbi request reprint Truncation of blood curves to enhance imaging and therapy with monoclonal antibodies
    L E Williams
    Division of Radiology, City of Hope National Medical Center, Beckman Research Institute, Duarte, California 91010, USA
    Med Phys 27:988-94. 2000
  9. ncbi request reprint Radioiodinated versus radiometal-labeled anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments: optimal pharmacokinetics for therapy
    Vania Kenanova
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Department of Radioimmunotherapy, Duarte, CA, USA
    Cancer Res 67:718-26. 2007
  10. ncbi request reprint Improved biodistribution and radioimmunoimaging with poly(ethylene glycol)-DOTA-conjugated anti-CEA diabody
    Lin Li
    Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Bioconjug Chem 17:68-76. 2006

Collaborators

Detail Information

Publications26

  1. pmc A series of anti-CEA/anti-DOTA bispecific antibody formats evaluated for pre-targeting: comparison of tumor uptake and blood clearance
    Paul J Yazaki
    Department of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Protein Eng Des Sel 26:187-93. 2013
    ..This pre-targeted radiotherapeutic and substantial reduction in the radioactive exposure to the bone marrow should enhance the therapeutic potential of RIT...
  2. pmc Biodistribution and tumor imaging of an anti-CEA single-chain antibody-albumin fusion protein
    Paul J Yazaki
    Division of Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Nucl Med Biol 35:151-8. 2008
    ..The radiometal-labeled version, which shows lower normal tissue accumulation than these recombinant antibodies, provides a promising and novel platform for antibody-based imaging agents...
  3. ncbi request reprint Humanization of the anti-CEA T84.66 antibody based on crystal structure data
    Paul J Yazaki
    Department of Radioimmunotherapy, Division of Radiology, City of Hope National Medical Center, 1500 East Duarte Road, Duarte, CA 91010, USA
    Protein Eng Des Sel 17:481-9. 2004
    ..66's ability to support clinical trials. Planned clinical trials will determine the effective utilization of this structure-based approach in the development of a promising new therapeutic...
  4. ncbi request reprint Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical applications
    P J Yazaki
    Department of Molecular Biology, Beckman Research Institute of the City of Hope, 91010, Duarte, CA, USA
    J Immunol Methods 253:195-208. 2001
    ..4 g of the T84.66 minibody in the harvest. A portion of this material was purified yielding 180 mg of highly purified T84.66 minibody intended for pilot radioimmunoscintigraphy studies of carcinoembryonic antigen (CEA) positive disease...
  5. ncbi request reprint Tumor targeting of radiometal labeled anti-CEA recombinant T84.66 diabody and t84.66 minibody: comparison to radioiodinated fragments
    P J Yazaki
    Department of Molecular Biology and Division of Immunology of the Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Bioconjug Chem 12:220-8. 2001
    ..Development of alternative blocking strategies or new metabolizable chelates are under investigation to enhance the utility of the radiometal form of these and other promising recombinant antibody fragments...
  6. ncbi request reprint Numerical selection of optimal tumor imaging agents with application to engineered antibodies
    L E Williams
    Division of Radiology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA, USA
    Cancer Biother Radiopharm 16:25-35. 2001
    ..The diabody had the largest impulse response function, but renal uptake may limit its present usefulness...
  7. pmc High-resolution microPET imaging of carcinoembryonic antigen-positive xenografts by using a copper-64-labeled engineered antibody fragment
    A M Wu
    Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 97:8495-500. 2000
    ..Average target/background ratios relative to neighboring tissue were 3-4:1. Engineered antibody fragments labeled with positron-emitting isotopes such as copper-64 provide a new class of agents for PET imaging of tumors...
  8. ncbi request reprint Truncation of blood curves to enhance imaging and therapy with monoclonal antibodies
    L E Williams
    Division of Radiology, City of Hope National Medical Center, Beckman Research Institute, Duarte, California 91010, USA
    Med Phys 27:988-94. 2000
    ..Use of such convolution techniques to assess truncation of the perfused material is probably also applicable to multistep processes as well as to lesion targeting with other tumor-specific pharmaceuticals...
  9. ncbi request reprint Radioiodinated versus radiometal-labeled anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments: optimal pharmacokinetics for therapy
    Vania Kenanova
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Department of Radioimmunotherapy, Duarte, CA, USA
    Cancer Res 67:718-26. 2007
    ..Finally, the 125I/111In biodistribution data allowed for dose estimations, which suggest the 131I-labeled scFv-Fc H310A/H435Q as a promising candidate for radioimmunotherapy...
  10. ncbi request reprint Improved biodistribution and radioimmunoimaging with poly(ethylene glycol)-DOTA-conjugated anti-CEA diabody
    Lin Li
    Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Bioconjug Chem 17:68-76. 2006
    ....
  11. ncbi request reprint Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments
    Vania Kenanova
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA
    Cancer Res 65:622-31. 2005
    ..Thus, attenuating the Fc-FcRn interaction provides a way of controlling the antibody fragment serum half-life without compromising expression and tumor targeting...
  12. ncbi request reprint Designer genes: recombinant antibody fragments for biological imaging
    A M Wu
    Department of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Q J Nucl Med 44:268-83. 2000
    ..Ease of engineering and expression, combined with novel specificities that will arise from advances in genomic and combinatorial approaches to target discovery, will usher in a new era of recombinant antibodies for biological imaging...
  13. pmc Monodispersed DOTA-PEG-conjugated anti-TAG-72 diabody has low kidney uptake and high tumor-to-blood ratios resulting in improved 64Cu PET
    Lin Li
    Department of Immunology, Beckman Research Institute of City of Hope, Duarte, California 91010, USA
    J Nucl Med 51:1139-46. 2010
    ..However, because of their high retention and metabolism in the kidney, the use of radiometal-labeled diabodies can be problematic for both imaging and therapy...
  14. pmc A versatile bifunctional chelate for radiolabeling humanized anti-CEA antibody with In-111 and Cu-64 at either thiol or amino groups: PET imaging of CEA-positive tumors with whole antibodies
    Lin Li
    Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Bioconjug Chem 19:89-96. 2008
    ....
  15. ncbi request reprint A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells
    C C Shih
    Department of Hematology Bone Marrow Transplantation, City of Hope National Medical Center, Duarte, CA 91010, USA
    Blood 95:1957-66. 2000
    ..Blood. 2000;95:1957-1966)..
  16. ncbi request reprint Pilot trial evaluating an 123I-labeled 80-kilodalton engineered anticarcinoembryonic antigen antibody fragment (cT84.66 minibody) in patients with colorectal cancer
    Jeffrey Y C Wong
    Division of Radiation Oncology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Clin Cancer Res 10:5014-21. 2004
    ..66 minibody demonstrated tumor targeting and was well tolerated as well as to begin to evaluate its biodistribution, pharmacokinetics, and immunogenicity in patients with colorectal cancer...
  17. ncbi request reprint A Phase I trial of 90Y-anti-carcinoembryonic antigen chimeric T84.66 radioimmunotherapy with 5-fluorouracil in patients with metastatic colorectal cancer
    Jeffrey Y C Wong
    Division of Radiation Oncology, Beckman Research Institute and City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Clin Cancer Res 9:5842-52. 2003
    ..The primary objective of this trial was to evaluate the feasibility and toxicities of systemic 90Y-chimeric T84.66 (cT84.66) anti-carcinoembryonic antigen RIT in combination with continuous infusion 5-fluorouracil (5-FU)...
  18. ncbi request reprint A phase I trial of (90)Y-DOTA-anti-CEA chimeric T84.66 (cT84.66) radioimmunotherapy in patients with metastatic CEA-producing malignancies
    Jeffrey Y C Wong
    City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Cancer Biother Radiopharm 21:88-100. 2006
    ..The aim of this phase I therapy trial was to evaluate cT84.66 conjugated to the macrocyclic chelate (90)Y-DOTA and labeled with (90)Y in a comparable patient population...
  19. pmc Neural stem cells as a novel platform for tumor-specific delivery of therapeutic antibodies
    Richard T Frank
    Division of Hematology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California, USA
    PLoS ONE 4:e8314. 2009
    ..Therefore, we hypothesized that NSCs could serve as an ideal cellular delivery platform for targeting antibodies to malignant tumors...
  20. ncbi request reprint Construction and characterization of minibodies for imaging and therapy of colorectal carcinomas
    Paul J Yazaki
    Department of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA
    Methods Mol Biol 207:351-64. 2003
  21. ncbi request reprint Expression of recombinant antibodies in mammalian cell lines
    Paul J Yazaki
    Beckman Research Institute of the City of Hope National Medical Center, Duarte, CA, USA
    Methods Mol Biol 248:255-68. 2004
  22. ncbi request reprint Optimizing radiolabeled engineered anti-p185HER2 antibody fragments for in vivo imaging
    Tove Olafsen
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at University of California at Los Angeles, 90095, USA
    Cancer Res 65:5907-16. 2005
    ..1 +/- 1.5% ID/g). Thus, by manipulating the size and format of anti-p185(HER2) antibody fragments, the kidney activity was reduced and high or low expression of p185HER2 in xenografts could be distinguished by microPET imaging...
  23. ncbi request reprint Covalent disulfide-linked anti-CEA diabody allows site-specific conjugation and radiolabeling for tumor targeting applications
    Tove Olafsen
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095
    Protein Eng Des Sel 17:21-7. 2004
    ..This format will provide a versatile platform for targeting a variety of agents to CEA-positive tumors...
  24. ncbi request reprint 124I-labeled engineered anti-CEA minibodies and diabodies allow high-contrast, antigen-specific small-animal PET imaging of xenografts in athymic mice
    Gobalakrishnan Sundaresan
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    J Nucl Med 44:1962-9. 2003
    ..The present work evaluated the minibody or diabody labeled with (124)I, for imaging tumor-bearing mice using a high-resolution small-animal PET system...
  25. ncbi request reprint The crystal structure of an anti-CEA scFv diabody assembled from T84.66 scFvs in V(L)-to-V(H) orientation: implications for diabody flexibility
    Jennifer A Carmichael
    CSIRO Health Sciences and Nutrition, 343 Royal Parade, Parkville 3052, Vic, Australia
    J Mol Biol 326:341-51. 2003
    ..This analysis therefore provides some signposts that may impact on future molecular designs for these therapeutic molecules with respect to diabody flexibility and avidity...
  26. ncbi request reprint Characterization of engineered anti-p185HER-2 (scFv-CH3)2 antibody fragments (minibodies) for tumor targeting
    Tove Olafsen
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 700 Westwood Plaza, Los Angeles, CA 90095, USA
    Protein Eng Des Sel 17:315-23. 2004
    ..62 h) was similar. Thus, minibodies with different specificities display similar pharmacokinetics, while tumor uptake may vary depending on the antigen-antibody system...