Binghui Shen

Summary

Affiliation: City of Hope National Medical Center
Country: USA

Publications

  1. pmc The DNA-protein interaction modes of FEN-1 with gap substrates and their implication in preventing duplication mutations
    Ren Liu
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 34:1772-84. 2006
  2. pmc Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding
    Zhigang Guo
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California, USA
    Nat Chem Biol 6:766-73. 2010
  3. pmc Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center, Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 37:3431-41. 2009
  4. pmc Functional regulation of FEN1 nuclease and its link to cancer
    Li Zheng
    Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Hangzhou, China
    Nucleic Acids Res 39:781-94. 2011
  5. ncbi request reprint Multiple but dissectible functions of FEN-1 nucleases in nucleic acid processing, genome stability and diseases
    Binghui Shen
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Bioessays 27:717-29. 2005
  6. pmc Disruption of the FEN-1/PCNA interaction results in DNA replication defects, pulmonary hypoplasia, pancytopenia, and newborn lethality in mice
    Li Zheng
    City of Hope National Medical Center, Department of Molecular Biology, 1500 East Duarte Rd, Duarte, CA 91010, USA
    Mol Cell Biol 27:3176-86. 2007
  7. ncbi request reprint Dual negative regulatory mechanisms of RecX on RecA functions in radiation resistance, DNA recombination and consequent genome instability in Deinococcus radiodurans
    Duohong Sheng
    Institute of Nuclear Agricultural Sciences, Zhejiang University, 310029 Hangzhou, China
    DNA Repair (Amst) 4:671-8. 2005
  8. ncbi request reprint Complementary functions of the Saccharomyces cerevisiae Rad2 family nucleases in Okazaki fragment maturation, mutation avoidance, and chromosome stability
    Xuemin Sun
    Division of Molecular Biology, City of Hope National Medical Center, Duarte, CA 91010, USA
    DNA Repair (Amst) 2:925-40. 2003
  9. pmc Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, CA 91010, USA
    Mol Cell Biol 28:4310-9. 2008
  10. ncbi request reprint Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli
    Guanjun Gao
    Institute of Nuclear Agricultural Science, Zhejiang University, 310029, China
    DNA Repair (Amst) 2:1419-27. 2003

Collaborators

Detail Information

Publications44

  1. pmc The DNA-protein interaction modes of FEN-1 with gap substrates and their implication in preventing duplication mutations
    Ren Liu
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 34:1772-84. 2006
    ..Furthermore, based on the evidence that GEN activity in complex with WRN protein cleaves hairpin and internal loop substrates, we suggest that the GEN activity may prevent repeat expansions and duplication mutations...
  2. pmc Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding
    Zhigang Guo
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California, USA
    Nat Chem Biol 6:766-73. 2010
    ..This consequently leads to a defect in Okazaki fragment maturation, a delay in cell cycle progression, impairment of DNA repair and a high frequency of genome-wide mutations...
  3. pmc Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center, Beckman Research Institute, Duarte, CA 91010, USA
    Nucleic Acids Res 37:3431-41. 2009
    ..These data indicate that polymorphisms in base excision repair genes may contribute to the onset and development of cancers...
  4. pmc Functional regulation of FEN1 nuclease and its link to cancer
    Li Zheng
    Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Hangzhou, China
    Nucleic Acids Res 39:781-94. 2011
    ..Lastly, we also review the biological significance of FEN1 as a tumor suppressor, with an emphasis on studies of human mutations and mouse models...
  5. ncbi request reprint Multiple but dissectible functions of FEN-1 nucleases in nucleic acid processing, genome stability and diseases
    Binghui Shen
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Bioessays 27:717-29. 2005
    ..This review summarizes the knowledge gained through efforts in the past decade to define its structural elements for specific activities and possible pathological consequences of altered functions of this multirole player...
  6. pmc Disruption of the FEN-1/PCNA interaction results in DNA replication defects, pulmonary hypoplasia, pancytopenia, and newborn lethality in mice
    Li Zheng
    City of Hope National Medical Center, Department of Molecular Biology, 1500 East Duarte Rd, Duarte, CA 91010, USA
    Mol Cell Biol 27:3176-86. 2007
    ..All newborn FFAA mutant pups died at birth, likely due to pulmonary hypoplasia and pancytopenia. Collectively, our data demonstrate the importance of the FEN-1/PCNA complex in DNA replication and in the embryonic development of mice...
  7. ncbi request reprint Dual negative regulatory mechanisms of RecX on RecA functions in radiation resistance, DNA recombination and consequent genome instability in Deinococcus radiodurans
    Duohong Sheng
    Institute of Nuclear Agricultural Sciences, Zhejiang University, 310029 Hangzhou, China
    DNA Repair (Amst) 4:671-8. 2005
    ..These data suggests a dual negative regulatory control of RecX on RecA functions in D. radiodurans...
  8. ncbi request reprint Complementary functions of the Saccharomyces cerevisiae Rad2 family nucleases in Okazaki fragment maturation, mutation avoidance, and chromosome stability
    Xuemin Sun
    Division of Molecular Biology, City of Hope National Medical Center, Duarte, CA 91010, USA
    DNA Repair (Amst) 2:925-40. 2003
    ..Furthermore, Rad2 and Exo1 complemented the mutator phenotypes and cell cycle defects of rad27 mutant strains to differing extents, suggesting distinct in vivo nucleic acid substrates...
  9. pmc Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, CA 91010, USA
    Mol Cell Biol 28:4310-9. 2008
    ..The replacement of Ser(187) by Ala, eliminating the only phosphorylation site, retains FEN1 in nucleoli. Both of the mutations cause UV sensitivity, impair cellular UV damage repair capacity, and decline overall cellular survivorship...
  10. ncbi request reprint Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli
    Guanjun Gao
    Institute of Nuclear Agricultural Science, Zhejiang University, 310029, China
    DNA Repair (Amst) 2:1419-27. 2003
    ..These results indicate that exogenous expression of Deira-PprI promotes DNA repair and protection pathways and enhances the radioresistance of E. coli...
  11. pmc Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways
    Zhigang Guo
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Mol Biol 377:679-90. 2008
    ..Replacement of one or more residues with an alanine in many cases leads to the complete loss of interaction, which may consequently lead to severe biological defects in mammals...
  12. pmc Human DNA2 is a mitochondrial nuclease/helicase for efficient processing of DNA replication and repair intermediates
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Mol Cell 32:325-36. 2008
    ..Taken together, our studies illustrate an evolutionarily diversified role of hDNA2 in mitochondrial DNA replication and repair in a mammalian system...
  13. ncbi request reprint Interaction and stimulation of human FEN-1 nuclease activities by heterogeneous nuclear ribonucleoprotein A1 in alpha-segment processing during Okazaki fragment maturation
    Qing Chai
    Division of Molecular Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Biochemistry 42:15045-52. 2003
    ..With longer flap substrates the hnRNP A1 effect is more modest and is suppressed by the addition of RPA. A model is provided to explain the possible in vivo role of this interaction and activity in Okazaki fragment maturation...
  14. ncbi request reprint Distinct roles of two Mg2+ binding sites in regulation of murine flap endonuclease-1 activities
    Li Zheng
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Biochemistry 41:10323-31. 2002
    ..Taken together, our results suggest distinct roles for two Mg(2+) binding sites in the regulation of mFEN-1 nuclease activities in a mode different from the "two-metal mechanism"...
  15. ncbi request reprint Three tandem HRDC domains have synergistic effect on the RecQ functions in Deinococcus radiodurans
    Lifen Huang
    Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China
    DNA Repair (Amst) 6:167-76. 2007
    ..Based on these data, we propose that the C-terminus of Dr1289 has evolved in D. radiodurans to confront the types and amounts of DNA damage...
  16. pmc The 3'-flap pocket of human flap endonuclease 1 is critical for substrate binding and catalysis
    L David Finger
    Division of Radiation Biology, Graduate School of Biological Sciences, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Biol Chem 284:22184-94. 2009
    ..Together the results indicate that the presence of a 3'-flap is the critical feature for efficient hFEN1 substrate recognition and catalysis...
  17. ncbi request reprint The retinitis pigmentosa-mutated RP2 protein exhibits exonuclease activity and translocates to the nucleus in response to DNA damage
    Jung Hoon Yoon
    Division of Biology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA
    Exp Cell Res 312:1323-34. 2006
    ..The data suggest that RP2 may have previously unrecognized roles as a DNA damage response factor and 3' to 5' exonuclease...
  18. ncbi request reprint Search for apoptotic nucleases in yeast: role of Tat-D nuclease in apoptotic DNA degradation
    Junzhuan Qiu
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    J Biol Chem 280:15370-9. 2005
    ..cerevisiae increases the TUNEL-positive cells and cell survival in response to hydrogen hyperoxide treatment, whereas overexpression of Tat-D facilitates cell death. These results suggest a role of Tat-D in yeast apoptosis...
  19. pmc Deinococcus radiodurans PprI switches on DNA damage response and cellular survival networks after radiation damage
    Huiming Lu
    Institute of Nuclear Agricultural Sciences, Key Laboratory of Chinese Ministry of Agriculture for Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China
    Mol Cell Proteomics 8:481-94. 2009
    ..These results suggest a role, either directly or indirectly, for PprI as a general switch to efficiently enhance the DNA repair capability and extreme radioresistance of D. radiodurans via regulation of a series of pathways...
  20. pmc Fen1 mutations that specifically disrupt its interaction with PCNA cause aneuploidy-associated cancer
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Cell Res 21:1052-67. 2011
    ..Thus, this study establishes an exemplary case for investigating the biological significance of protein-protein interactions by knock-in of a point mutation rather than knock-out of a whole gene...
  21. ncbi request reprint Fen1 mutations result in autoimmunity, chronic inflammation and cancers
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, California 91010, USA
    Nat Med 13:812-9. 2007
    ....
  22. pmc Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity
    Weiqiang Lin
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    EMBO J 32:1425-39. 2013
    ....
  23. ncbi request reprint Concerted action of exonuclease and Gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG)n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments
    Purnima Singh
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    J Biol Chem 282:3465-77. 2007
    ..A model is presented to explain how the concerted action of EXO and GEN activities may contribute to resolving structured flaps, thereby preventing their expansion in the genome...
  24. ncbi request reprint Interaction interface of human flap endonuclease-1 with its DNA substrates
    Junzhuan Qiu
    Department of Radiation Research, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    J Biol Chem 279:24394-402. 2004
    ..R., Hosfield, D. J., Han, S., Qiu, J., Yelent, B., Shen, B., Tainer, J. A. (2004) Cell 116, 39-50) we are able to propose a reasonable model for how the human FEN-1 protein interacts with its DNA substrates...
  25. ncbi request reprint Arginine residues 47 and 70 of human flap endonuclease-1 are involved in DNA substrate interactions and cleavage site determination
    Junzhuan Qiu
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    J Biol Chem 277:24659-66. 2002
    ....
  26. pmc Polyploid cells rewire DNA damage response networks to overcome replication stress-induced barriers for tumour progression
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, 1500 East Duarte Road, Duarte, California 91010, USA
    Nat Commun 3:815. 2012
    ..These molecular changes rewire DNA damage response and repair gene networks in polyploid tumour cells, enabling them to escape replication stress-induced senescence barriers...
  27. pmc Novel function of the flap endonuclease 1 complex in processing stalled DNA replication forks
    Li Zheng
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    EMBO Rep 6:83-9. 2005
    ..Replication protein A facilitates FEN-1 interaction with DNA bubble structures. Human FEN-1, but not the GEN-deficient mutant, E178A, was shown to rescue the defect in resistance to UV and camptothecin in a yeast FEN-1 null mutant...
  28. pmc Characterization of the 3' --> 5' exonuclease activity found in human nucleoside diphosphate kinase 1 (NDK1) and several of its homologues
    Jung Hoon Yoon
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Biochemistry 44:15774-86. 2005
    ..These results suggest that, in addition to maintenance of nucleotide pool balance, the human NDK-like proteins may have previously unrecognized roles in DNA nucleolytic processing...
  29. pmc High risk of benzo[α]pyrene-induced lung cancer in E160D FEN1 mutant mice
    Zhenxing Wu
    Colleges of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    Mutat Res 731:85-91. 2012
    ..All together, our current study suggests that individuals carrying the GEN-deficient FEN1 mutations have high risk to develop lung cancer upon exposure to B[α]P-containing agents such as tobacco smoke...
  30. ncbi request reprint LexA analog (dra0074) is a regulatory protein that is irrelevant to recA induction
    Duohong Sheng
    Institute of Nuclear Agricultural Sciences, Zhejiang University, 310029, Hangzhou, China
    J Biochem 136:787-93. 2004
    ..Results showed that the product of gene dra0074 is not involved in RecA induction, but is a regulator of other metabolisms in D. radiodurans...
  31. ncbi request reprint Suppression of Saccharomyces cerevisiae rad27 null mutant phenotypes by the 5' nuclease domain of Escherichia coli DNA polymerase I
    Xuemin Sun
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    Curr Genet 41:379-88. 2002
    ..We conclude that the N-terminal nuclease domain of E. coli polymerase I encodes a functional homologue of FEN-1...
  32. pmc Overexpression and hypomethylation of flap endonuclease 1 gene in breast and other cancers
    Purnima Singh
    Department of Radiation Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Mol Cancer Res 6:1710-7. 2008
    ..The overexpression and promoter hypomethylation of FEN1 may serve as biomarkers for monitoring the progression of cancers...
  33. pmc Okazaki fragment maturation: nucleases take centre stage
    Li Zheng
    Department of Cancer Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010, USA
    J Mol Cell Biol 3:23-30. 2011
    ..On the other hand, mutations that impair editing out of polymerase α incorporation errors result in cancer displaying a strong mutator phenotype...
  34. ncbi request reprint Functional alterations of human exonuclease 1 mutants identified in atypical hereditary nonpolyposis colorectal cancer syndrome
    Xuemin Sun
    Division of Molecular Medicine, City of Hope National Medical Center and Beckman Research Institute, Duarte, California 91010, USA
    Cancer Res 62:6026-30. 2002
    ..The combination of these three point mutations leads to the binding capacity with hMsh2 to nearly zero. Evidence made available in this study sheds light on the pathogenesis of HNPCC, perhaps initiated by an additional MMR gene, hEXO1...
  35. pmc The DNase domain-containing protein TATDN1 plays an important role in chromosomal segregation and cell cycle progression during zebrafish eye development
    Hui Yang
    College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China
    Cell Cycle 11:4626-32. 2012
    ..Altogether, our current studies suggest that zTATDN1 plays an important role in chromosome segregation and eye development in zebrafish...
  36. ncbi request reprint Human 3-methyladenine-DNA glycosylase: effect of sequence context on excision, association with PCNA, and stimulation by AP endonuclease
    Liqun Xia
    Biology Division, Beckman Research Institute, City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    J Mol Biol 346:1259-74. 2005
    ..Thus, PCNA could have a role in short-patch BER as well as in long-patch BER. Overall, the data reported here show how multiple factors contribute to the activity of MPG in cells...
  37. pmc Reciprocal regulation of Akt and Oct4 promotes the self-renewal and survival of embryonal carcinoma cells
    Yuanji Lin
    State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, Zhejiang 310003, China
    Mol Cell 48:627-40. 2012
    ....
  38. ncbi request reprint PprI: a general switch responsible for extreme radioresistance of Deinococcus radiodurans
    Yuejin Hua
    Institute of Nuclear Agricultural Science, Zhejiang University, 310029, Hangzhou, China
    Biochem Biophys Res Commun 306:354-60. 2003
    ..These results strongly suggest that PprI plays a crucial role in regulating multiple DNA repair and protection pathways in response to radiation stress...
  39. pmc Sequential posttranslational modifications program FEN1 degradation during cell-cycle progression
    Zhigang Guo
    Jiangsu Key Laboratory for Molecular and Medical Biotechnology and College of Life Sciences, Nanjing Normal University, Nanjing 210046, China
    Mol Cell 47:444-56. 2012
    ..These findings may represent a newly identified regulatory mechanism used by cells to ensure precise cell-cycle progression and to prevent transformation...
  40. pmc Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease 1
    Muralidhar L Hegde
    Department of Biochemistry and Molecular Biology, University of Texas, Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 283:27028-37. 2008
    ..Interaction between NEIL1 and FEN-1 is essential for efficient NEIL1-initiated LP-BER. These studies strongly implicate NEIL1 in a distinct subpathway of LP-BER in replicating genomes...
  41. ncbi request reprint Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair
    Brian R Chapados
    Department of Molecular Biology and Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92122, USA
    Cell 116:39-50. 2004
    ....
  42. pmc CRN-1, a Caenorhabditis elegans FEN-1 homologue, cooperates with CPS-6/EndoG to promote apoptotic DNA degradation
    Jay Z Parrish
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    EMBO J 22:3451-60. 2003
    ..Our results suggest that CRN-1/FEN-1 may play a critical role in switching the state of cells from DNA replication/repair to DNA degradation during apoptosis...
  43. pmc Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria
    Pingfang Liu
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Mol Cell Biol 28:4975-87. 2008
    ..The mitochondrial LP-BER pathway likely plays important roles in repairing dL lesions and other oxidative lesions and perhaps in normal mtDNA replication...
  44. ncbi request reprint Mutational spectra of p53 in geographically localized esophageal squamous cell carcinoma groups in China
    Wei Cao
    Department of Pathology, Xinxiang Medical College, Xinxiang, Henan, People s Republic of China
    Cancer 101:834-44. 2004
    ..Epidemiologic data have suggested that many environmental exposures may be associated with an increased risk of its formation...

Research Grants26

  1. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2010
    ..The information made available via these studies will be useful in developing new strategies for cancer prevention and treatment. ..
  2. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2005
    ..Information made available from this systematic study will also establish a relationship between this mechanism, unique mutagenic phenotype(s), and development of genetic diseases. ..
  3. Function Analysis of FEN-1 Nuclease in Genome Stability
    Binghui Shen; Fiscal Year: 2006
    ..abstract_text> ..
  4. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2006
    ..Information made available from this systematic study will also establish a relationship between this mechanism, unique mutagenic phenotype(s), and development of genetic diseases. ..
  5. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2007
    ..Information made available from this systematic study will also establish a relationship between this mechanism, unique mutagenic phenotype(s), and development of genetic diseases. ..
  6. Functional Analysis of FEN-1 Nuclease in Genome Stability and Cancers
    Binghui Shen; Fiscal Year: 2007
    ..Completion of this research will not only clarify the molecular mechanisms underlying cancer pathology, but also provide a solid foundation for development of new regimens for cancer therapy. ..
  7. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2009
    ..The information made available via these studies will be useful in developing new strategies for cancer prevention and treatment. ..
  8. Functional Analysis of FEN-1 Nuclease in Genome Stability and Cancers
    Binghui Shen; Fiscal Year: 2009
    ..Completion of this research will not only clarify the molecular mechanisms underlying cancer pathology, but also provide a solid foundation for development of new regimens for cancer therapy. ..
  9. Functional Analysis of FEN-1 Nuclease in Genome Stability and Cancers
    Binghui Shen; Fiscal Year: 2010
    ..Completion of this research will not only clarify the molecular mechanisms underlying cancer pathology, but also provide a solid foundation for development of new regimens for cancer therapy. ..
  10. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2009
    ..Moreover, the proposed research will have immediate economic impact by creating 1 postdoctoral position and retaining a graduate student and covering efforts of two key existing scientists. ..
  11. Function Analysis of FEN-1 Nuclease in Genome Stability
    Binghui Shen; Fiscal Year: 2005
    ..abstract_text> ..
  12. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2004
    ..Information made available from this systematic study will also establish a relationship between this mechanism, unique mutagenic phenotype(s), and development of genetic diseases. ..
  13. Function Analysis of FEN-1 Nuclease in Genome Stability
    Binghui Shen; Fiscal Year: 2002
    ..abstract_text> ..
  14. Function Analysis of FEN-1 Nuclease in Genome Stability
    Binghui Shen; Fiscal Year: 2003
    ..abstract_text> ..
  15. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2003
    ..Information made available from this systematic study will also establish a relationship between this mechanism, unique mutagenic phenotype(s), and development of genetic diseases. ..
  16. Function Analysis of FEN-1 Nuclease in Genome Stability
    Binghui Shen; Fiscal Year: 2004
    ..abstract_text> ..
  17. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2003
    ..Information made available from this systematic study will also establish a relationship between this mechanism, unique mutagenic phenotype(s), and development of genetic diseases. ..
  18. Role of Nucleases in RNA Primer Removal and Mutagenesis
    Binghui Shen; Fiscal Year: 2011
    ..The information made available via these studies will be useful in developing new strategies for cancer prevention and treatment. ..