GERD PFEIFER

Summary

Affiliation: City of Hope National Medical Center
Country: USA

Publications

  1. ncbi 5-hydroxymethylcytosine and its potential roles in development and cancer
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute, City of Hope, 1500 East Duarte Road, Duarte, CA, 91010, USA
    Epigenetics Chromatin 6:10. 2013
  2. ncbi Methylated-CpG island recovery assay-assisted microarrays for cancer diagnosis
    Gerd P Pfeifer
    Beckman Research Institute of the City of Hope, Division of Biology, Duarte, CA 91010, USA 1 626 301 8853 1 626 358 7703
    Expert Opin Med Diagn 1:99-108. 2007
  3. ncbi A genome-wide screen identifies frequently methylated genes in haematological and epithelial cancers
    Thomas Dunwell
    Department of Medical and Molecular Genetics, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham B152TT, UK
    Mol Cancer 9:44. 2010
  4. ncbi Identification of 5 novel genes methylated in breast and other epithelial cancers
    Victoria K Hill
    Department of Medical and Molecular Genetics, University of Birmingham, UK
    Mol Cancer 9:51. 2010
  5. ncbi Methylation of homeobox genes is a frequent and early epigenetic event in breast cancer
    Stella Tommasi
    Department of Cancer Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Breast Cancer Res 11:R14. 2009
  6. ncbi Mutagenesis at methylated CpG sequences
    G P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Curr Top Microbiol Immunol 301:259-81. 2006
  7. ncbi UV wavelength-dependent DNA damage and human non-melanoma and melanoma skin cancer
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Photochem Photobiol Sci 11:90-7. 2012
  8. ncbi DNA methylation patterns in lung carcinomas
    Gerd P Pfeifer
    Department of Biology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA
    Semin Cancer Biol 19:181-7. 2009
  9. ncbi Mutational spectra of human cancer
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Hum Genet 125:493-506. 2009
  10. ncbi Environmental exposures and mutational patterns of cancer genomes
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Genome Med 2:54. 2010

Research Grants

  1. RASSF1 Tumor Suppressor Gene
    GERD PFEIFER; Fiscal Year: 2005
  2. Characterization of MBD3L1 and MBD3L2
    GERD PFEIFER; Fiscal Year: 2007
  3. CPG METHYLATION AND MUTATION
    GERD PFEIFER; Fiscal Year: 2007
  4. UV damage, repair, and mutagenesis
    GERD PFEIFER; Fiscal Year: 2007
  5. DNA methylation in cancer genomes
    GERD PFEIFER; Fiscal Year: 2007
  6. CPG METHYLATION AND MUTATION
    Gerd P Pfeifer; Fiscal Year: 2010
  7. UV DAMAGE AND REPAIR AT THE DNA SEQUENCE LEVEL
    GERD PFEIFER; Fiscal Year: 1993
  8. UV DAMAGE, REPAIR AND MUTAGENESIS
    GERD PFEIFER; Fiscal Year: 2003
  9. CPG METHYLATION AND MUTATION
    GERD PFEIFER; Fiscal Year: 2003
  10. Aging and the unstable epigenome
    Gerd P Pfeifer; Fiscal Year: 2010

Collaborators

Detail Information

Publications92

  1. ncbi 5-hydroxymethylcytosine and its potential roles in development and cancer
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute, City of Hope, 1500 East Duarte Road, Duarte, CA, 91010, USA
    Epigenetics Chromatin 6:10. 2013
    ..We discuss current data and models of the function of 5hmC in general, with special emphasis on its role in mechanisms of development and cancer...
  2. ncbi Methylated-CpG island recovery assay-assisted microarrays for cancer diagnosis
    Gerd P Pfeifer
    Beckman Research Institute of the City of Hope, Division of Biology, Duarte, CA 91010, USA 1 626 301 8853 1 626 358 7703
    Expert Opin Med Diagn 1:99-108. 2007
    ..These methods are described briefly and the methylated-CpG island recovery assay will be reviewed. This assay has been used in combination with microarray analysis to map CpG island methylation across cancer genomes...
  3. ncbi A genome-wide screen identifies frequently methylated genes in haematological and epithelial cancers
    Thomas Dunwell
    Department of Medical and Molecular Genetics, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham B152TT, UK
    Mol Cancer 9:44. 2010
    ..High throughput screens are required to identify epigenetic markers that can be useful for diagnostic and prognostic purposes across malignancies...
  4. ncbi Identification of 5 novel genes methylated in breast and other epithelial cancers
    Victoria K Hill
    Department of Medical and Molecular Genetics, University of Birmingham, UK
    Mol Cancer 9:51. 2010
    ..We utilized one such recently developed approach, MIRA (methylated-CpG island recovery assay) combined with CpG island arrays to identify novel genes that are epigenetically inactivated in breast cancer...
  5. ncbi Methylation of homeobox genes is a frequent and early epigenetic event in breast cancer
    Stella Tommasi
    Department of Cancer Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Breast Cancer Res 11:R14. 2009
    ....
  6. ncbi Mutagenesis at methylated CpG sequences
    G P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Curr Top Microbiol Immunol 301:259-81. 2006
    ..Certain polycyclic aromatic hydrocarbons form guanine adducts and induce G to T transversion mutations with high selectivity at mCpG sequences...
  7. ncbi UV wavelength-dependent DNA damage and human non-melanoma and melanoma skin cancer
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Photochem Photobiol Sci 11:90-7. 2012
    ..Collectively, the data implicate the CPD as the DNA lesion most strongly involved in human cancers induced by sunlight...
  8. ncbi DNA methylation patterns in lung carcinomas
    Gerd P Pfeifer
    Department of Biology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA
    Semin Cancer Biol 19:181-7. 2009
    ..Since these epigenetic changes are found in early stage tumors, their contribution to tumor etiology as well as their potential usefulness as diagnostic or prognostic biomarkers of the disease should be considered...
  9. ncbi Mutational spectra of human cancer
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Hum Genet 125:493-506. 2009
    ..Unraveling the origin of these G to C mutations will be of importance for understanding cancer etiology...
  10. ncbi Environmental exposures and mutational patterns of cancer genomes
    Gerd P Pfeifer
    Department of Cancer Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Genome Med 2:54. 2010
    ..Two recent studies of melanoma and small cell lung cancer exemplify what type of information can be gained from cancer genome sequencing...
  11. ncbi p53 mutational spectra and the role of methylated CpG sequences
    G P Pfeifer
    Department of Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA
    Mutat Res 450:155-66. 2000
    ..These sequences are particularly prone to mutagenesis involving endogenous events as well as modification by exogenous carcinogens...
  12. ncbi Mutations induced by ultraviolet light
    Gerd P Pfeifer
    Department of Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Mutat Res 571:19-31. 2005
    ..We also discuss the role of DNA damage-tolerant DNA polymerases in UV lesion bypass and mutagenesis...
  13. ncbi On the origin of G --> T transversions in lung cancer
    Gerd P Pfeifer
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Mutat Res 526:39-43. 2003
    ....
  14. ncbi Methylation of the tumor suppressor gene RASSF1A in human tumors
    G P Pfeifer
    Department of Biology, Beckman Research Institute, City of Hope Cancer Center, Duarte, CA 91010, USA
    Biochemistry (Mosc) 70:576-83. 2005
    ..The functional analysis of RASSF1A reveals a potential involvement of this protein in apoptotic signaling, microtubule stabilization, and cell cycle progression...
  15. ncbi Tobacco smoke carcinogens, DNA damage and p53 mutations in smoking-associated cancers
    Gerd P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California, CA 91010, USA
    Oncogene 21:7435-51. 2002
    ..The available data suggest that p53 mutations in lung cancers can be attributed to direct DNA damage from cigarette smoke carcinogens rather than to selection of pre-existing endogenous mutations...
  16. ncbi Similarities in sunlight-induced mutational spectra of CpG-methylated transgenes and the p53 gene in skin cancer point to an important role of 5-methylcytosine residues in solar UV mutagenesis
    Y H You
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    J Mol Biol 305:389-99. 2001
    ....
  17. ncbi Methylated CpG dinucleotides are the preferential targets for G-to-T transversion mutations induced by benzo[a]pyrene diol epoxide in mammalian cells: similarities with the p53 mutation spectrum in smoking-associated lung cancers
    J H Yoon
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Cancer Res 61:7110-7. 2001
    ....
  18. ncbi Characterization of novel parent-specific epigenetic modifications upstream of the imprinted mouse H19 gene
    P E Szabo
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Mol Cell Biol 18:6767-76. 1998
    ..We therefore reveal the existence of novel parent-specific epigenetic modifications, which in addition to DNA methylation, could constitute imprints or maintain monoallelic expression of H19...
  19. ncbi Epigenetic inactivation of a RAS association domain family protein from the lung tumour suppressor locus 3p21.3
    R Dammann
    Department of Biology, Beckman Research Institute, City of Hope Medical Center, Duarte, California, USA
    Nat Genet 25:315-9. 2000
    ..These characteristics indicate a potential role for RASSF1A as a lung tumour suppressor gene...
  20. ncbi In vivo protein-DNA interactions at the c-jun promoter: preformed complexes mediate the UV response
    D Rozek
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010
    Mol Cell Biol 13:5490-9. 1993
    ..Smeal, and M. Karin, Cell 71:1081-1091, 1992). Taken together, these data suggest that modification of the transactivating domain of DNA-bound c-Jun or a closely related factor may trigger the rapid induction of the c-jun gene...
  21. ncbi Hypermethylation of the cpG island of Ras association domain family 1A (RASSF1A), a putative tumor suppressor gene from the 3p21.3 locus, occurs in a large percentage of human breast cancers
    R Dammann
    Department of Biology, Beckman Research Institute, City of Hope Cancer Center, Duarte, California 91010, USA
    Cancer Res 61:3105-9. 2001
    ..No somatic mutations were found in the samples that were unmethylated. The data suggest that hypermethylation of the CpG island promoter of RASSF1A may play an important role in breast cancer pathogenesis...
  22. ncbi In vivo structure of two divergent promoters at the human PCNA locus. Synthesis of antisense RNA and S phase-dependent binding of E2F complexes in intron 1
    S Tommasi
    Department of Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010, USA
    J Biol Chem 274:27829-38. 1999
    ..The binding of S phase-specific E2F complexes at the +583 element may help to overcome the negative effect of the antisense transcript, which results in up-regulation of PCNA expression in proliferating cells...
  23. ncbi Formation and repair of DNA lesions in the p53 gene: relation to cancer mutations?
    G P Pfeifer
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Environ Mol Mutagen 31:197-205. 1998
    ..Future studies with suspected mutagens may help to implicate causative agents involved in other cancers, such as colon and breast cancer, where the exact carcinogen has not yet been identified but an environmental factor is suspected...
  24. ncbi Genomic structure and mutation screening of the E2F4 gene in human tumors
    S Schwemmle
    Department of Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010, USA
    Int J Cancer 86:672-7. 2000
    ..Our data suggest that a direct involvement of E2F4 in tumorigenesis is unlikely, although increased E2F4 expression may be associated with human cancer...
  25. ncbi PCR-based approaches to adduct analysis
    G P Pfeifer
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Toxicol Lett 102:447-51. 1998
    ..Future studies with suspected mutagens may help to implicate causative agents involved in other cancers, where the exact carcinogen has not yet been identified but an environmental factor is suspected...
  26. ncbi Formation and processing of UV photoproducts: effects of DNA sequence and chromatin environment
    G P Pfeifer
    Department of Biology, Beckman Institute of the City of Hope, Duarte, CA 91010, USA
    Photochem Photobiol 65:270-83. 1997
    ..To understand the mechanisms of UV mutagenesis, it is important to know how these lesions are formed, by which cellular pathways they are repaired and how they are dealt with by DNA polymerases...
  27. ncbi Constitutive protection of E2F recognition sequences in the human thymidine kinase promoter during cell cycle progression
    S Tommasi
    Department of Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010, USA
    J Biol Chem 272:30483-90. 1997
    ....
  28. ncbi Maternal-specific footprints at putative CTCF sites in the H19 imprinting control region give evidence for insulator function
    P Szabo
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010 3011, USA
    Curr Biol 10:607-10. 2000
    ..These results strongly suggest that the maternal ICR functions as an insulator element in regulating mutually exclusive expression of Igf2 and H19 in cis...
  29. ncbi Genomic sequencing and methylation analysis by ligation mediated PCR
    G P Pfeifer
    Molecular Biology Section, Beckman Research Institute of the City of Hope, Duarte, CA 91010
    Science 246:810-3. 1989
    ....
  30. ncbi Cell cycle-independent removal of UV-induced pyrimidine dimers from the promoter and the transcription initiation domain of the human CDC2 gene
    S Tommasi
    Department of Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA
    Nucleic Acids Res 28:3991-8. 2000
    ..We suggest that sequences involved in transcription initiation may be book-marked for efficient repair throughout the cell cycle, even when the gene is temporarily not expressed...
  31. ncbi The CpG island of the novel tumor suppressor gene RASSF1A is intensely methylated in primary small cell lung carcinomas
    R Dammann
    Department of Biology, Beckman Research Institute, City of Hope Cancer Center, Duarte, California 91010, USA
    Oncogene 20:3563-7. 2001
    ..None of the SCLCs showed evidence for methylation of the CpG island of RASSF1C. The results suggest that hypermethylation of the CpG island promoter of the RASSF1A gene is associated with SCLC pathogenesis...
  32. ncbi Cyclobutane pyrimidine dimers are responsible for the vast majority of mutations induced by UVB irradiation in mammalian cells
    Y H You
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 276:44688-94. 2001
    ..We conclude that CPDs are responsible for at least 80% of the UVB-induced mutations in this mammalian cell model...
  33. ncbi Hypermethylation of the CpG island of the RASSF1A gene in ovarian and renal cell carcinomas
    J H Yoon
    Department of Biology, Beckman Research Institute, City of Hope Cancer Center, Duarte, CA 91010, USA
    Int J Cancer 94:212-7. 2001
    ..This data suggests that methylation of the CpG island and promoter of the RASSF1A gene is common not only in lung and breast tumors but also in renal cell carcinoma and ovarian cancer...
  34. ncbi Fluorescence-based directed termination PCR: direct mutation characterization without sequencing
    J Z Chen
    Department of Biology, City of Hope National Medical Center and Beckman Research Institute, 1450 East Duarte Road, Duarte, CA 91010, USA
    Nucleic Acids Res 29:E17. 2001
    ..We anticipate that the automated DT-PCR method will serve as a cost-effective alternative to dideoxy sequencing in studies involving large-scale analysis for nucleotide sequence changes...
  35. ncbi RASSF1A interacts with and activates the mitotic kinase Aurora-A
    L Liu
    Division of Biology, Beckman Research Institute, City of Hope Cancer Center, Duarte, CA 91010, USA
    Oncogene 27:6175-86. 2008
    ..Inhibition of centrosome separation by RASSF1A overexpression is most likely a consequence of hyperstabilization of microtubules by this protein...
  36. ncbi In vivo assays for transcription-coupled repair
    Graciela Spivak
    Department of Biological Sciences, Stanford University, California, USA
    Methods Enzymol 408:223-46. 2006
    ..Both methods have unique advantages and limitations for particular applications...
  37. ncbi The histone methyltransferase SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells
    Hongwei Li
    Division of Biology, Beckman Research Institute, City of Hope, Duarte, California 91010, USA
    J Biol Chem 281:19489-500. 2006
    ....
  38. ncbi Measuring the formation and repair of DNA damage by ligation-mediated PCR
    Gerd P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA
    Methods Mol Biol 314:201-14. 2006
    ..I describe the technical details of LM-PCR as exemplified by the mapping of DNA damage products in ultraviolet (UV) light-irradiated cells...
  39. ncbi Investigating human cancer etiology by DNA lesion footprinting and mutagenicity analysis
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA 91010, USA
    Carcinogenesis 27:1526-37. 2006
    ..We present examples of application of each of these methodologies to human cancer etiology, and provide prospective views on investigations using these technologies for carcinogenicity testing...
  40. ncbi Involvement of the RASSF1A tumor suppressor gene in controlling cell migration
    Ashraf Dallol
    Section of Medical and Molecular Genetics, Institute of Biomedical Research, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    Cancer Res 65:7653-9. 2005
    ..These findings represent a novel function for RASSF1A, which may help explain its tumor suppression ability independently of its effects on cell cycle and apoptosis...
  41. ncbi Methylated-CpG island recovery assay: a new technique for the rapid detection of methylated-CpG islands in cancer
    Tibor Rauch
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Lab Invest 85:1172-80. 2005
    ..MIRA is a specific and sensitive, but not laborious, technique that can be clinically useful in the detection and diagnosis of any DNA methylation-associated disease, including cancer...
  42. ncbi DNA lesions induced by UV A1 and B radiation in human cells: comparative analyses in the overall genome and in the p53 tumor suppressor gene
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute, and Department of Medical Oncology, The City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 102:10058-63. 2005
    ..We conclude that UVA1 induces promutagenic CPDs and oxidative DNA damage at both the genomic and nucleotide resolution level in normal human skin fibroblasts...
  43. ncbi Short hairpin RNA-directed cytosine (CpG) methylation of the RASSF1A gene promoter in HeLa cells
    Daniela Castanotto
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Mol Ther 12:179-83. 2005
    ..The results presented suggest provocative potential mechanisms for transcriptional gene silencing via DNA methylation in cancer cells...
  44. ncbi The tumor suppressor RASSF1A and MAP-1 link death receptor signaling to Bax conformational change and cell death
    Shairaz Baksh
    Cancer Biology Program, Division of Hematology and Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, 1038 NRB, 330 Brookline Avenue, Boston, Massachusetts 02215, USA
    Mol Cell 18:637-50. 2005
    ..Our findings identify RASSF1A and MAP-1 as important components between death receptors and the apoptotic machinery and reveal a potential link between tumor suppression and death receptor signaling...
  45. ncbi MIRA-assisted microarray analysis, a new technology for the determination of DNA methylation patterns, identifies frequent methylation of homeodomain-containing genes in lung cancer cells
    Tibor Rauch
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Cancer Res 66:7939-47. 2006
    ..The results support the hypothesis that cancer-associated DNA methylation events do not occur randomly throughout the genome but at least some are targeted by specific mechanisms...
  46. ncbi Mutagenesis induced by the nitric oxide donor sodium nitroprusside in mouse cells
    Dong Hyun Lee
    Division of Biology, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA
    Mutagenesis 22:63-7. 2007
    ..003) but the proportion of transition mutations was not increased. We discuss the potential origin of the G-->T mutations induced by this compound in mammalian cells...
  47. ncbi Second-hand smoke and human lung cancer
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, Duarte, CA 91010, USA
    Lancet Oncol 9:657-66. 2008
    ..This Review is a synopsis of research on SHS and lung cancer, with special focus on hypothetical modes of action of SHS for carcinogenesis, including genotoxic and epigenetic effects...
  48. ncbi Sunlight ultraviolet irradiation and BRAF V600 mutagenesis in human melanoma
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, Duarte, California 91010, USA
    Hum Mutat 29:983-91. 2008
    ....
  49. ncbi Translesion synthesis of 7,8-dihydro-8-oxo-2'-deoxyguanosine by DNA polymerase eta in vivo
    Dong Hyun Lee
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, United States
    Mutat Res 641:19-26. 2008
    ..These results show that Pol eta has an important role in error-free 8-oxo-dG lesion bypass and avoidance of oxidative stress-induced mutagenesis in vivo...
  50. ncbi Rapid repair of UVA-induced oxidized purines and persistence of UVB-induced dipyrimidine lesions determine the mutagenicity of sunlight in mouse cells
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    FASEB J 22:2379-92. 2008
    ..The prevailing role of UVB over UVA in solar mutagenesis in our test system can be ascribed to different kinetics of repair for lesions induced by the respective UV irradiation...
  51. ncbi High-resolution mapping of DNA hypermethylation and hypomethylation in lung cancer
    Tibor A Rauch
    Divisions of Biology, Information Sciences, and Surgery, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 105:252-7. 2008
    ..The results are consistent with a specific defect in methylation of repetitive DNA sequences in human cancer...
  52. ncbi Lack of mutagenicity of acrolein-induced DNA adducts in mouse and human cells
    Sang In Kim
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, Duarte, California 91010 3000, USA
    Cancer Res 67:11640-7. 2007
    ..We conclude that acrolein is not mutagenic to mouse and human fibroblasts, regardless of DNA repair capacity or methylation status of CpGs, possibly because of a highly accurate replication bypass of the induced lesions...
  53. ncbi Riboflavin activated by ultraviolet A1 irradiation induces oxidative DNA damage-mediated mutations inhibited by vitamin C
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 104:5953-8. 2007
    ..Our findings support the notion that UVA-induced photosensitization reactions are responsible for oxidative DNA damage leading to mutagenesis...
  54. ncbi RASSF1A is part of a complex similar to the Drosophila Hippo/Salvador/Lats tumor-suppressor network
    Cai Guo
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Curr Biol 17:700-5. 2007
    ..RASSF1A, MST2, or WW45 can rescue this defect. The complex of RASSF1A, MST2, WW45, and LATS1 consists of several tumor suppressors, is conserved in mammalian cells, and appears to be involved in controlling mitotic exit...
  55. ncbi Homeobox gene methylation in lung cancer studied by genome-wide analysis with a microarray-based methylated CpG island recovery assay
    Tibor Rauch
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Proc Natl Acad Sci U S A 104:5527-32. 2007
    ..The finding of widespread methylation of homeobox genes lends support to the hypothesis that a substantial fraction of genes methylated in human cancer are targets of the Polycomb complex...
  56. ncbi Mutagenicity of ultraviolet A radiation in the lacI transgene in Big Blue mouse embryonic fibroblasts
    Sang In Kim
    Division of Biology, Beckman Research Institute of City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    Mutat Res 617:71-8. 2007
    ....
  57. ncbi A review of mechanisms of acrylamide carcinogenicity
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    Carcinogenesis 28:519-28. 2007
    ..Future directions for research on acrylamide and cancer are outlined, and potential challenges are underscored...
  58. ncbi Investigating DNA adduct-targeted mutagenicity of tamoxifen: preferential formation of tamoxifen-DNA adducts in the human p53 gene in SV40 immortalized hepatocytes but not endometrial carcinoma cells
    Ahmad Besaratinia
    Department of Biology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, California 91010, USA
    Biochemistry 44:8418-27. 2005
    ..Our findings favor an involvement of a nongenotoxic mechanism in tamoxifen-associated human endometrial cancer...
  59. ncbi DNA sequence analysis of 1-nitropyrene-4,5-oxide and 1-nitropyrene-9,10-oxide induced mutations in the hprt gene of Chinese hamster ovary cells
    Hyun-Jo Kim
    Department of Biochemistry, College of Natural Sciences, Yeungnam University, Gyongsan 712-749, Korea
    Mol Cells 19:114-23. 2005
    ....
  60. ncbi Frequent RASSF1A promoter hypermethylation and K-ras mutations in pancreatic carcinoma
    Reinhard Dammann
    AG Tumorgenetik der Medizinischen Fakultät, Martin Luther Universitat Halle Wittenberg, 06097 Halle Saale, Germany
    Oncogene 22:3806-12. 2003
    ..001, chi(2) test). In conclusion, our data indicate that inactivation of the RASSF1A gene is a frequent event in pancreatic cancer and suggest an inverse correlation between RASSF1A silencing and K-ras activation...
  61. ncbi Inactivation of RAS association domain family 1A gene in cervical carcinomas and the role of human papillomavirus infection
    Igor Kuzmin
    Basic Research Program, Science Applications International Corporation Frederick, Inc, NCI Frederick, Frederick, Maryland, 21702, USA
    Cancer Res 63:1888-93. 2003
    ..Our data imply that the presence of HPVs in cervical carcinomas alleviates the requirement for RASSF1A inactivation and suggests that these two events may engage the same tumorigenic pathway...
  62. ncbi Epigenetic inactivation of RAS association domain family protein 1 (RASSF1A) in malignant cutaneous melanoma
    Mia Spugnardi
    Department of Molecular Oncology, John Wayne Cancer Institute, Saint John s Health Center, Santa Monica, California 90404, USA
    Cancer Res 63:1639-43. 2003
    ..Our findings indicate that the RASSF1A gene is turned off in a significant number of melanomas and that CpG promoter region hypermethylation may play a role in the transcriptional inactivation of the RASSF1A gene in malignant melanoma...
  63. ncbi Frequent epigenetic inactivation of the RASSF1A gene in hepatocellular carcinoma
    Undraga Schagdarsurengin
    , , Halle/Saale, Germany
    Oncogene 22:1866-71. 2003
    ..Thus, RASSF1A hypermethylation could be useful as a marker of malignancy and to distinguish between the distinct forms of highly differentiated liver neoplasm...
  64. ncbi Deamination of 5-methylcytosines within cyclobutane pyrimidine dimers is an important component of UVB mutagenesis
    Dong-Hyun Lee
    Division of Biology, Beckman Research Institute, City of Hope, Duarte, California 91010, USA
    J Biol Chem 278:10314-21. 2003
    ..This particular mutagenic pathway may become prevalent under conditions of inefficient DNA repair and slow proliferation of cells in the human epidermis...
  65. ncbi Mutations induced by 1,3-butadiene metabolites, butadiene diolepoxide, and 1,2,3,4-diepoxybutane at the Hprt locus in CHO-K1 cells
    Dong-Hyun Lee
    Department of Biochemistry and Institute of Natural Sciences, College of Natural Sciences, Yeungnam University, Kyongsan 712-749, Korea
    Mol Cells 14:411-9. 2002
    ..These data indicate that BDE and DEB are mutagenic carcinogens by forming DNA adducts at the site of adenine and guanine, and inducing large exon deletions and single base substitutions...
  66. ncbi MBD3L1 and MBD3L2, two new proteins homologous to the methyl-CpG-binding proteins MBD2 and MBD3: characterization of MBD3L1 as a testis-specific transcriptional repressor
    Chun Ling Jiang
    Department of Biology, Duarte, California 91010, USA
    Genomics 80:621-9. 2002
    ..Expression of MBD3L2 was found in germ cell tumors and some somatic tissues. These novel proteins may function as counterparts of MBD2 and/or MBD3 in developmental stage-specific transcriptional repression...
  67. ncbi Frequent hypermethylation of the RASSF1A gene in prostate cancer
    Limin Liu
    Department of Biology, Beckman Research Institute, City of Hope Cancer Center, Duarte, California, CA 91010, USA
    Oncogene 21:6835-40. 2002
    ..Testing for RASSF1A methylation should become useful in prostate cancer early detection and diagnosis and might aid prognosis by gauging the potential status of progression...
  68. ncbi Methylation of the RASSF1A gene in human cancers
    Gerd P Pfeifer
    Department of Biology, City of Hope Medical Center, Duarte, CA 91010, USA
    Biol Chem 383:907-14. 2002
    ..A high frequency of methylation of RASSF1A is found also in breast cancers, renal cell carcinomas, ovarian, gastric and bladder cancers, and in neuroblastomas. The RASSF1A gene is a candidate for a tumor suppressor gene in 3p21.3...
  69. ncbi Oxidative DNA damage induced by copper and hydrogen peroxide promotes CG-->TT tandem mutations at methylated CpG dinucleotides in nucleotide excision repair-deficient cells
    Dong-Hyun Lee
    Department of Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA
    Nucleic Acids Res 30:3566-73. 2002
    ..We suggest that the underlying oxidative lesions could be responsible for the progressive neurodegeneration seen in XP-A individuals...
  70. ncbi Mutational signature of the proximate bladder carcinogen N-hydroxy-4-acetylaminobiphenyl: inconsistency with the p53 mutational spectrum in bladder cancer
    Ahmad Besaratinia
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Cancer Res 62:4331-8. 2002
    ..However, the pattern of mutation induced by N-OH-AABP in the cII gene is largely at odds with the mutational spectrum of the p53 gene in human bladder cancer...
  71. ncbi Frequent epigenetic silencing of the CpG island promoter of RASSF1A in thyroid carcinoma
    Undraga Schagdarsurengin
    , , Halle/Saale, Germany
    Cancer Res 62:3698-701. 2002
    ..However, RASSF1A hypermethylation was detected three times more frequently in thyroid cancers. Thus, RASSF1A inactivation may play a crucial role in the malignancy of thyroid carcinoma...
  72. ncbi Mapping polycyclic aromatic hydrocarbon and aromatic amine-induced DNA damage in cancer-related genes at the sequence level
    Moon shong Tang
    Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA
    Int J Hyg Environ Health 205:103-13. 2002
    ..Here we present the rationale and details of this approach, typical experimental results and necessary precautions...
  73. ncbi RASSF3 and NORE1: identification and cloning of two human homologues of the putative tumor suppressor gene RASSF1
    Stella Tommasi
    Biology Department, Beckman Research Institute of the City of Hope, Duarte, California, CA 91010, USA
    Oncogene 21:2713-20. 2002
    ..However, aberrations in tumors have so far not been found. The presence of a Ras association domain common to NORE1, RASSF1, and RASSF3 suggests their possible involvement in Ras-like signaling pathways...
  74. ncbi Methylation analysis by chemical DNA sequencing
    PIROSKA E SZABO
    Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USA
    Methods Mol Biol 200:29-41. 2002
  75. ncbi Weak yet distinct mutagenicity of acrylamide in mammalian cells
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, Duarte, CA 91010, USA
    J Natl Cancer Inst 95:889-96. 2003
    ..Despite concern raised with the announcement that common heating processes such as frying introduce acrylamide, a known rodent carcinogen, into food, the mutagenicity of acrylamide in mammalian DNA is controversial...
  76. ncbi Human thymine DNA glycosylase (TDG) and methyl-CpG-binding protein 4 (MBD4) excise thymine glycol (Tg) from a Tg:G mispair
    Jung-Hoon Yoon
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Nucleic Acids Res 31:5399-404. 2003
    ..This repair pathway contributes to mutation avoidance at methylated CpG dinucleotides...
  77. ncbi MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing
    Seung Gi Jin
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 280:12700-9. 2005
    ..Thus, MBD3L2 has the potential to recruit the MeCP1 complex away from methylated DNA and reactivate transcription...
  78. ncbi DNA adduction and mutagenic properties of acrylamide
    Ahmad Besaratinia
    Department of Biology, Beckman Research Institute of the City of Hope, National Medical Center, 1450 East Duarte Road, Duarte, CA 91010, USA
    Mutat Res 580:31-40. 2005
    ..Prospective views are provided on the potential applications of acrylamide-induced DNA adduct dosimetry/mapping and mutation frequency/spectrometry for biomonitoring purposes...
  79. ncbi Tumor susceptibility of Rassf1a knockout mice
    Stella Tommasi
    Division of Biology, City of Hope National Medical Center, Duarte, CA 91010, USA
    Cancer Res 65:92-8. 2005
    ..The data are consistent with the tumor-suppressive role of Rassf1a, which may explain its frequent epigenetic inactivation in human tumors...
  80. ncbi Biological consequences of 8-methoxypsoralen-photoinduced lesions: sequence-specificity of mutations and preponderance of T to C and T to a mutations
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, Duarte, California, USA
    J Invest Dermatol 123:1140-6. 2004
    ....
  81. ncbi The role of DNA polymerase eta in UV mutational spectra
    Jun-Hyuk Choi
    Division of Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    DNA Repair (Amst) 4:211-20. 2005
    ..We provide proof of principle that siRNA technology can be used to dissect the in vivo roles of lesion bypass DNA polymerases in DNA damage-induced mutagenesis...
  82. ncbi A methylation profile of in vitro immortalized human cell lines
    Limin Liu
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Int J Oncol 26:275-85. 2005
    ..In general, more genes were methylated in neoplastically-transformed cell lines than in only immortalized cell lines, indicating that accumulation of epigenetic abnormalities may contribute to oncogenesis...
  83. ncbi Similar mutagenicity of photoactivated porphyrins and ultraviolet A radiation in mouse embryonic fibroblasts: involvement of oxidative DNA lesions in mutagenesis
    Ahmad Besaratinia
    Division of Biology and Department of Medical Oncology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, California 91010, USA
    Biochemistry 43:15557-66. 2004
    ..We conclude that, in the presence of exogenous photosensitizers, UVA at a nonmutagenic dose induces mutations through the same mechanism as does a mutagenic dose of UVA per se...
  84. ncbi Peroxyl radical mediated oxidative DNA base damage: implications for lipid peroxidation induced mutagenesis
    Punnajit Lim
    Division of Molecular Biology and Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, California 91010, USA
    Biochemistry 43:15339-48. 2004
    ..Evidence is presented for the involvement of 8-oxodG in the oxidation of DNA by ROO(*). These results are used to interpret some key features of previously published mutation spectra induced by lipid peroxidation in human cells...
  85. ncbi DNA damage and mutations produced by chloroacetaldehyde in a CpG-methylated target gene
    Jun-Hyuk Choi
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Mutat Res 568:245-56. 2004
    ..We conclude that methylated CpG sites are not preferential targets for chloroacetaldehyde-induced mutagenesis...
  86. ncbi DNA damage, repair, and mutation induction by (+)-Syn and (-)-anti-dibenzo[a,l]pyrene-11,12-diol-13,14-epoxides in mouse cells
    Jung-Hoon Yoon
    Beckman Research Institute of the City of Hope, Duarte, California, USA
    Cancer Res 64:7321-8. 2004
    ..We discuss the implications of these findings for human cancer mutagenesis...
  87. ncbi Genotoxicity of acrylamide and glycidamide
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, Duarte, CA 91010, USA
    J Natl Cancer Inst 96:1023-9. 2004
    ..Acrylamide, a known rodent carcinogen, is found in the human diet. However, the mechanism by which acrylamide exerts its carcinogenic effects remains unclear...
  88. ncbi G-to-T transversions and small tandem base deletions are the hallmark of mutations induced by ultraviolet a radiation in mammalian cells
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, 1450 East Duarte Road, Duarte, California 91010, USA
    Biochemistry 43:8169-77. 2004
    ..We conclude that UVA has the potential to trigger carcinogenesis owing to its mutagenic effects mediated through oxidative DNA damage...
  89. ncbi Parent-of-origin-specific binding of nuclear hormone receptor complexes in the H19-Igf2 imprinting control region
    PIROSKA E SZABO
    Division of Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA
    Mol Cell Biol 24:4858-68. 2004
    ..These data are consistent with the possibility that nuclear hormone receptor complexes participate in the establishment of differential ICR methylation imprinting in the germ line...
  90. ncbi Enhancement of the mutagenicity of benzo(a)pyrene diol epoxide by a nonmutagenic dose of ultraviolet A radiation
    Ahmad Besaratinia
    Division of Biology, Beckman Research Institute of the City of Hope National Medical Center, Duarte, California 91010 3000, USA
    Cancer Res 63:8708-16. 2003
    ..We conclude that UVA radiation at a nonmutagenic dose has an enhancing effect on the mechanism by which B(a)PDE induces mutations...
  91. ncbi Control of microtubule stability by the RASSF1A tumor suppressor
    Limin Liu
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA
    Oncogene 22:8125-36. 2003
    ..In addition, knowledge of the microtubule-targeting function of RASSF1 may aid in the development of new anticancer drugs...
  92. ncbi The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1
    Sara Ortiz-Vega
    Diabetes Unit and Medical Services, Massachusetts General Hospital, Boston, Massachusetts, MA 02114, USA
    Oncogene 21:1381-90. 2002
    ..The preferential ability of RASSF1A to heterodimerize with Nore and thereby associate with Ras-like GTPases may be relevant to its putative tumor suppressor function...

Research Grants37

  1. RASSF1 Tumor Suppressor Gene
    GERD PFEIFER; Fiscal Year: 2005
    ..The effect of RASSF1 expression on RAS signaling pathways will be studied. We will examine if RASSF1A, alone or in combination with activated RAS, induces cell death (apoptosis). ..
  2. Characterization of MBD3L1 and MBD3L2
    GERD PFEIFER; Fiscal Year: 2007
    ..Since both proteins are likely functioning as transcriptional regulators, it will be important to determine if there are changes in gene expression patterns in Mbd311 and Mbd312 (-/-) cells. ..
  3. CPG METHYLATION AND MUTATION
    GERD PFEIFER; Fiscal Year: 2007
    ..These approaches should increase our understanding of the basic mechanisms leading to the most common type of single base substitution in mammalian cells. ..
  4. UV damage, repair, and mutagenesis
    GERD PFEIFER; Fiscal Year: 2007
    ....
  5. DNA methylation in cancer genomes
    GERD PFEIFER; Fiscal Year: 2007
    ..In this R21 application, we will further refine the methodology and attempt to apply the MIRA approach for microarray-based screening of genome-wide DNA methylation differences between normal lung tissue and early stage lung tumors. ..
  6. CPG METHYLATION AND MUTATION
    Gerd P Pfeifer; Fiscal Year: 2010
    ..In parallel, we will study molecular pathways involving the Polycomb repression complex that might operate during tumor progression to promote hypermethylation of CpG islands in malignant tissue. ..
  7. UV DAMAGE AND REPAIR AT THE DNA SEQUENCE LEVEL
    GERD PFEIFER; Fiscal Year: 1993
    ....
  8. UV DAMAGE, REPAIR AND MUTAGENESIS
    GERD PFEIFER; Fiscal Year: 2003
    ..abstract_text> ..
  9. CPG METHYLATION AND MUTATION
    GERD PFEIFER; Fiscal Year: 2003
    ..3) The PI will investigate whether C->T hotspots at CpGs might be due to one of four endogenous mutagens, which might generate mutations preferentially at m5C residues. ..
  10. Aging and the unstable epigenome
    Gerd P Pfeifer; Fiscal Year: 2010
    ..Genome-wide analysis will be accomplished by microarray and high throughput DNA sequence analysis. ..