Jeffrey Whitsett

Summary

Affiliation: Cincinnati Children's Hospital Medical Center
Country: USA

Publications

  1. pmc Maternal synchronization of gestational length and lung maturation
    Valérie Besnard
    The Perinatal Institute and Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, the Department of Pediatrics and The University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America
    PLoS ONE 6:e26682. 2011
  2. pmc Sox2 is required for maintenance and differentiation of bronchiolar Clara, ciliated, and goblet cells
    David H Tompkins
    Division of Pulmonary Biology in the Perinatal Institute, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America
    PLoS ONE 4:e8248. 2009
  3. pmc Gene expression and biological processes influenced by deletion of Stat3 in pulmonary type II epithelial cells
    Yan Xu
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH, USA
    BMC Genomics 8:455. 2007
  4. pmc Intersections between pulmonary development and disease
    Jeffrey A Whitsett
    Perinatal Institute, Divisions of Neonatology, Perinatal, and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Crit Care Med 184:401-6. 2011
  5. ncbi request reprint Genetic disorders influencing lung formation and function at birth
    Jeffrey A Whitsett
    Department of Pediatrics, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Hum Mol Genet 13:R207-15. 2004
  6. ncbi request reprint Surfactant proteins in innate host defense of the lung
    Jeffrey A Whitsett
    Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229 3039, USA
    Biol Neonate 88:175-80. 2005
  7. ncbi request reprint Disorders of lung morphogenesis
    Jeffrey A Whitsett
    Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, MLC 7029, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Paediatr Respir Rev 7:S248. 2006
  8. ncbi request reprint Transcriptional regulation of perinatal lung maturation
    Jeffrey A Whitsett
    Department of Pediatrics, Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, 3333 Burnet Avenue Cincinnati, OH 45229 3039, USA
    Pediatr Clin North Am 53:873-87, viii. 2006
  9. pmc Alveolar surfactant homeostasis and the pathogenesis of pulmonary disease
    Jeffrey A Whitsett
    Perinatal Institute, Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Annu Rev Med 61:105-19. 2010
  10. pmc Integrin α6β4 defines a novel lung epithelial progenitor cell: a step forward for cell-based therapies for pulmonary disease
    Jeffrey A Whitsett
    Perinatal Institute, Division of Neonatology, Perinatal, and Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Clin Invest 121:2543-5. 2011

Collaborators

Detail Information

Publications81

  1. pmc Maternal synchronization of gestational length and lung maturation
    Valérie Besnard
    The Perinatal Institute and Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, the Department of Pediatrics and The University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America
    PLoS ONE 6:e26682. 2011
    ..Ovarian transfer experiments demonstrated that maternal genotype determines the timing of birth and can influence fetal lung growth and maturation to ensure perinatal survival...
  2. pmc Sox2 is required for maintenance and differentiation of bronchiolar Clara, ciliated, and goblet cells
    David H Tompkins
    Division of Pulmonary Biology in the Perinatal Institute, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America
    PLoS ONE 4:e8248. 2009
    ..Sox2 is required for proliferation and differentiation of Clara cells that serve as the progenitor cells from which Clara, ciliated, and goblet cells are derived...
  3. pmc Gene expression and biological processes influenced by deletion of Stat3 in pulmonary type II epithelial cells
    Yan Xu
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH, USA
    BMC Genomics 8:455. 2007
    ..Differentially expressed genes were identified from Affymetrix Murine GeneChips analysis and subjected to gene ontology classification, promoter analysis, pathway mapping and literature mining...
  4. pmc Intersections between pulmonary development and disease
    Jeffrey A Whitsett
    Perinatal Institute, Divisions of Neonatology, Perinatal, and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Crit Care Med 184:401-6. 2011
    ....
  5. ncbi request reprint Genetic disorders influencing lung formation and function at birth
    Jeffrey A Whitsett
    Department of Pediatrics, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Hum Mol Genet 13:R207-15. 2004
    ..Inherited disorders of the surfactant system that affect neonatal respiratory adaptation at birth include hereditary surfactant protein B deficiency, mutations in surfactant protein C and the ABCA3 transporter...
  6. ncbi request reprint Surfactant proteins in innate host defense of the lung
    Jeffrey A Whitsett
    Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229 3039, USA
    Biol Neonate 88:175-80. 2005
    ..Pulmonary surfactant proteins A and D play dual roles in pulmonary homeostasis, determining the structure of alveolar lipids and mediating the innate host defense system of the lung...
  7. ncbi request reprint Disorders of lung morphogenesis
    Jeffrey A Whitsett
    Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, MLC 7029, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Paediatr Respir Rev 7:S248. 2006
    ..Perinatal lung function depends upon formation of the conducting and gas exchange regions of the lung. The precise expression of transcription factors and signaling molecules is required for normal lung morphogenesis...
  8. ncbi request reprint Transcriptional regulation of perinatal lung maturation
    Jeffrey A Whitsett
    Department of Pediatrics, Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, 3333 Burnet Avenue Cincinnati, OH 45229 3039, USA
    Pediatr Clin North Am 53:873-87, viii. 2006
    ....
  9. pmc Alveolar surfactant homeostasis and the pathogenesis of pulmonary disease
    Jeffrey A Whitsett
    Perinatal Institute, Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Annu Rev Med 61:105-19. 2010
    ....
  10. pmc Integrin α6β4 defines a novel lung epithelial progenitor cell: a step forward for cell-based therapies for pulmonary disease
    Jeffrey A Whitsett
    Perinatal Institute, Division of Neonatology, Perinatal, and Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Clin Invest 121:2543-5. 2011
    ....
  11. ncbi request reprint Review: The intersection of surfactant homeostasis and innate host defense of the lung: lessons from newborn infants
    Jeffrey A Whitsett
    Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Ohio 45229 3039, USA
    Innate Immun 16:138-42. 2010
    ....
  12. ncbi request reprint Genetic disorders of surfactant homeostasis
    Jeffrey A Whitsett
    Division of Pulmonary Biology, MLC 7029, Department of Pediatrics, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Paediatr Respir Rev 7:S240-2. 2006
    ..Mutations in the genes encoding these proteins cause lethal respiratory distress in newborn infants. This review discusses the clinical and pathological findings associated with these inherited disorders of alveolar homeostasis...
  13. ncbi request reprint Genetic disorders of surfactant homeostasis
    Jeffrey A Whitsett
    Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, The University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Biol Neonate 87:283-7. 2005
    ....
  14. ncbi request reprint Notch and basal cells take center stage during airway epithelial regeneration
    Jeffrey A Whitsett
    Perinatal Institute, Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Cell Stem Cell 8:597-8. 2011
    ..In this issue of Cell Stem Cell, Rock et al. (2011) show that Notch signals regulate differentiation, but not self-renewal of basal cells after injury, and that this role appears conserved in mouse and human lung...
  15. pmc SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells
    Taeko K Noah
    Division of Gastroenterology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, OH, USA
    Exp Cell Res 316:452-65. 2010
    ..Spdef has been reported to be expressed in the intestine. In this paper, we identify an important role for Spdef in regulating intestinal epithelial cell homeostasis and differentiation...
  16. pmc CTGF disrupts alveolarization and induces pulmonary hypertension in neonatal mice: implication in the pathogenesis of severe bronchopulmonary dysplasia
    Shaoyi Chen
    Department of Pediatrics, Division of Neonatology, Univ of Miami Miller School of Medicine, FL 33101, USA
    Am J Physiol Lung Cell Mol Physiol 300:L330-40. 2011
    ....
  17. pmc Wntless is required for peripheral lung differentiation and pulmonary vascular development
    Bridget Cornett
    The Perinatal Institute Division of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Cincinnati Children s Hospital Medical Center Research Foundation, Cincinnati, OH 45229, USA
    Dev Biol 379:38-52. 2013
    ..These studies provide a new framework for understanding the molecular mechanisms underlying normal pulmonary vasculature formation and the dysmorphic pulmonary vasculature development associated with congenital lung disease...
  18. pmc Conditional overexpression of connective tissue growth factor disrupts postnatal lung development
    Shu Wu
    Department of Pediatrics, Division of Neonatology, University of Miami Miller School of Medicine, Miami, Florida 33101, USA
    Am J Respir Cell Mol Biol 42:552-63. 2010
    ..These histologic changes are similar to those observed in lungs of infants with bronchopulmonary dysplasia...
  19. ncbi request reprint Inhaled nitric oxide for oligohydramnios-induced pulmonary hypoplasia: a report of two cases and review of the literature
    Cara Geary
    Divisions of Neonatology and Pulmonary Biology, Children s Hospital Medical Center and Good Samaritan Hospital, Cincinnati, OH 45229 3039, USA
    J Perinatol 22:82-5. 2002
    ....
  20. ncbi request reprint Absence of SP-A modulates innate and adaptive defense responses to pulmonary influenza infection
    Ann Marie LeVine
    Division of Pulmonary Biology, Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 282:L563-72. 2002
    ..Because the airway is the usual portal of entry for IAV and other respiratory pathogens, SP-A is likely to play a role in innate defense and adaptive immune responses to IAV...
  21. ncbi request reprint Surfactant replacement for ventilator-associated pneumonia: a preliminary report
    Robert P Baughman
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0564, USA
    Respiration 69:57-62. 2002
    ..Surfactant abnormalities have been described in bacterial pneumonia...
  22. pmc Cell-specific conditional deletion of Pten in the uterus results in differential phenotypes
    Takiko Daikoku
    Division of Reproductive Sciences, The Perinatal Institute, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Gynecol Oncol 122:424-9. 2011
    ..Our objective was to determine whether deletion of Pten specifically in the uterine stroma and myometrium induces cancer or manifests different phenotypes...
  23. ncbi request reprint TAZ interacts with TTF-1 and regulates expression of surfactant protein-C
    Kwon Sik Park
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 279:17384-90. 2004
    ..TAZ binds to TTF-1, increasing the transcriptional activity of TTF-1 on the SP-C promoter. Developmental and cell-selective regulation of TAZ provides a mechanism by which the activity of TTF-1 on target genes is modulated...
  24. doi request reprint Ectopic respiratory epithelial cell differentiation in bronchiolised distal airspaces in idiopathic pulmonary fibrosis
    Laurent Plantier
    Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Perinatal Institute, MLC7029, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Thorax 66:651-7. 2011
    ..Bronchiolisation of distal airspaces is an unexplained feature of idiopathic pulmonary fibrosis (IPF). The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process...
  25. pmc Sox2 activates cell proliferation and differentiation in the respiratory epithelium
    David H Tompkins
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, MLC 7029, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 45:101-10. 2011
    ..Sox2 induces proliferation of respiratory epithelial cells and, subsequently, partially reprograms alveolar epithelial cells into cells with characteristics of the conducting airways...
  26. pmc Conditional deletion of Abca3 in alveolar type II cells alters surfactant homeostasis in newborn and adult mice
    Valérie Besnard
    The Perinatal Institute and Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics and University of Cincinnati College of Medicine, Ohio
    Am J Physiol Lung Cell Mol Physiol 298:L646-59. 2010
    ..Compensatory surfactant synthesis was initiated in ABCA3-sufficient type II cells, indicating that surfactant homeostasis is a highly regulated process that includes sensing and coregulation among alveolar type II cells...
  27. ncbi request reprint R-spondin 2 is required for normal laryngeal-tracheal, lung and limb morphogenesis
    Sheila M Bell
    Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Development 135:1049-58. 2008
    ..Interbreeding the Rspo2(Tg) and Lrp6(-) alleles resulted in more severe defects consisting of marked lung hypoplasia and absence of tracheal-bronchial rings, laryngeal structures and all limb skeletal elements...
  28. doi request reprint Kruppel-like factor 5 is required for perinatal lung morphogenesis and function
    Huajing Wan
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 135:2563-72. 2008
    ..KLF5 regulates genes controlling paracrine interactions during lung morphogenesis, as well as those regulating the maturation of the respiratory epithelium that is required for lung function after birth...
  29. pmc A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration
    Yuzhen Zhang
    Department of Medicine, University of Pennsylvania, 956 BRB II III, 421 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA
    Nat Genet 40:862-70. 2008
    ..Together, these data demonstrate that Gata6-regulated Wnt signaling controls the balance between progenitor expansion and epithelial differentiation required for both lung development and regeneration...
  30. pmc GP130-STAT3 regulates epithelial cell migration and is required for repair of the bronchiolar epithelium
    Hiroshi Kida
    Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Am J Pathol 172:1542-54. 2008
    ..In the present study, we show that GP130-STAT3 signaling functions in a cell-autonomous manner to restore cell shape and numbers required for repair of the bronchiolar epithelium following injury...
  31. pmc Surfactant protein D alters allergic lung responses in mice and human subjects
    Eric B Brandt
    Division of Allergy and Immunology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    J Allergy Clin Immunol 121:1140-1147.e2. 2008
    ..Surfactant protein (SP) D has been proposed to be protective in allergic airway responses...
  32. pmc Prenatal lung epithelial cell-specific abrogation of Alk3-bone morphogenetic protein signaling causes neonatal respiratory distress by disrupting distal airway formation
    Jianping Sun
    Developmental Biology Program, Childrens Hospital Los Angeles, 4650 Sunset Blvd, MS 35, Los Angeles, CA 90027, USA
    Am J Pathol 172:571-82. 2008
    ..Therefore, our data suggest that deficiency of appropriate BMP signaling in lung epithelial cells results in prenatal lung malformation, neonatal atelectasis, and respiratory failure...
  33. ncbi request reprint Transcriptional adaptation to cystic fibrosis transmembrane conductance regulator deficiency
    Yan Xu
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 278:7674-82. 2003
    ..Absence of CFTR influenced the expression of genes modulating diverse pulmonary cell functions that may ameliorate or contribute to the pathogenesis of CF...
  34. pmc Alveolar epithelial STAT3, IL-6 family cytokines, and host defense during Escherichia coli pneumonia
    Lee J Quinton
    Molecular and Integrative Physiological Sciences Program, Harvard School of Public Health, 665 Huntington Ave, Boston, MA 02115, USA
    Am J Respir Cell Mol Biol 38:699-706. 2008
    ..Together, these results indicate that during E. coli pneumonia, select IL-6 family members activate alveolar epithelial STAT3, which functions to promote neutrophil recruitment and to limit both infection and lung injury...
  35. pmc STAT3 regulates ABCA3 expression and influences lamellar body formation in alveolar type II cells
    Yohei Matsuzaki
    Cincinnati Children s Hospital Medical Center, Section of Neonatology, Perinatal and Pulmonary Biology, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 38:551-8. 2008
    ..ABCA3 expression is regulated by IL-6 in a pathway that includes STAT3, PI3K, AKT, SCAP, and SREBP. Activation of STAT3 after exposure to IL-6 enhances ABCA3 expression, which, in turn, influences pulmonary surfactant homeostasis...
  36. ncbi request reprint Pneumonitis and emphysema in sp-C gene targeted mice
    Stephan W Glasser
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Ohio 45229 3039, USA
    J Biol Chem 278:14291-8. 2003
    ..Absence of SP-C caused a severe progressive pulmonary disorder with histologic features consistent with interstitial pneumonitis...
  37. pmc Pten controls lung morphogenesis, bronchioalveolar stem cells, and onset of lung adenocarcinomas in mice
    Shigehisa Yanagi
    Department of Molecular Biology, Akita University School of Medicine, Akita, Japan
    J Clin Invest 117:2929-40. 2007
    ..These results indicate that Pten is essential for both normal lung morphogenesis and the prevention of lung carcinogenesis, possibly because this tumor suppressor is required for BASC homeostasis...
  38. ncbi request reprint Sterol response element binding protein and thyroid transcription factor-1 (Nkx2.1) regulate Abca3 gene expression
    Valérie Besnard
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 293:L1395-405. 2007
    ..Abca3 gene expression is mediated by discrete cis-acting cassettes that mediate pulmonary cell- and lipid-sensitive pathways regulating surfactant homeostasis...
  39. ncbi request reprint Prenatal, but not postnatal, inhibition of fibroblast growth factor receptor signaling causes emphysema
    Isamu Hokuto
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 278:415-21. 2003
    ..5 but did not alter alveolarization when expressed after birth. Although FGF signaling was required for branching morphogenesis early in lung development, postnatal alveolarization was not influenced by FGFR-HFc...
  40. ncbi request reprint Aminoglycoside suppression of a premature stop mutation in a Cftr-/- mouse carrying a human CFTR-G542X transgene
    Ming Du
    Department of Microbiology, The University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    J Mol Med (Berl) 80:595-604. 2002
    ..052). When taken together, these results indicate that gentamicin, and to a lesser extent tobramycin, can restore the synthesis of functional hCFTR protein by suppressing the hCFTR-G542X premature stop mutation in vivo...
  41. ncbi request reprint Reversibility of pulmonary abnormalities by conditional replacement of surfactant protein D (SP-D) in vivo
    Liqian Zhang
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 277:38709-13. 2002
    ..However, once established, emphysema was not reversed by SP-D. SP-D-dependent processes regulating surfactant lipid homeostasis were disassociated from those mediating emphysema...
  42. ncbi request reprint GATA-6 and thyroid transcription factor-1 directly interact and regulate surfactant protein-C gene expression
    Cong Liu
    Division of Pulmonary Biology, Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 277:4519-25. 2002
    ..GATA-6 influenced the activity of the sp-C promoter, binding and acting synergistically with TTF-1...
  43. ncbi request reprint Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury
    Machiko Ikegami
    Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229 3039, USA
    J Appl Physiol (1985) 92:519-26. 2002
    ..Genetically decreased levels of SP-B combined with superimposed O(2)-induced injury reveals the distinct contribution of SP-C to pulmonary function in vivo...
  44. ncbi request reprint Increased expression of thyroid transcription factor-1 (TTF-1) in respiratory epithelial cells inhibits alveolarization and causes pulmonary inflammation
    Susan E Wert
    Division of Pulmonary Biology, Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    Dev Biol 242:75-87. 2002
    ..Increased expression of TTF-1 altered alveolarization and caused chronic pulmonary inflammation, demonstrating that precise regulation of TTF-1 is critical for homeostasis in the postnatal lung...
  45. ncbi request reprint Conditional gene expression in the respiratory epithelium of the mouse
    Anne Karina T Perl
    Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio, USA
    Transgenic Res 11:21-9. 2002
    ..The SP-C-rtTA and CCSP-rtTA activator mice control the expression of transgenes in the developing and mature respiratory epithelium, and will be useful for the study of gene function in the lung...
  46. ncbi request reprint Fibroblast growth factor 18 influences proximal programming during lung morphogenesis
    Jeffrey A Whitsett
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 277:22743-9. 2002
    ..Effects were unique to FGF-18 because expression of other members of the FGF family had different consequences. These data show that FGF-18 is capable of enhancing proximal and inhibiting peripheral programs during lung morphogenesis...
  47. ncbi request reprint Fusion of lung lobes and vessels in mouse embryos heterozygous for the forkhead box f1 targeted allele
    Lorena Lim
    Department of Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60607 7170, USA
    Am J Physiol Lung Cell Mol Physiol 282:L1012-22. 2002
    ....
  48. ncbi request reprint Complementation of pulmonary abnormalities in SP-D(-/-) mice with an SP-D/conglutinin fusion protein
    Liqian Zhang
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 277:22453-9. 2002
    ..Our findings also strongly suggest that the molecular mechanisms underlying impaired pulmonary host defense and abnormal lipid metabolism are distinct from those that promote ongoing inflammation and the development of emphysema...
  49. ncbi request reprint Wild-type levels of the mouse Forkhead Box f1 gene are essential for lung repair
    Vladimir V Kalinichenko
    Department of Molecular Genetics, College of Medicine, University of Illinois at Chicago, 60607 7170, USA
    Am J Physiol Lung Cell Mol Physiol 282:L1253-65. 2002
    ..Collectively, our data suggest that sustained expression of Foxf1 is essential for normal lung repair and endothelial cell survival in response to pulmonary cell injury...
  50. ncbi request reprint The role of pulmonary collectin N-terminal domains in surfactant structure, function, and homeostasis in vivo
    Nades Palaniyar
    Division of Pulmonary Critical Care Medicine, Department of Medicine, Children s Hospital Research Foundation, University of Cincinnati School of Medicine, Cincinnati, OH 45267 0564, USA
    J Biol Chem 277:26971-9. 2002
    ....
  51. ncbi request reprint GATA6 regulates differentiation of distal lung epithelium
    Honghua Yang
    Department of Medicine, Molecular Cardiology Research Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Development 129:2233-46. 2002
    ..Together, these data implicate GATA6 as an important regulator of distal epithelial cell differentiation and proximal airway development in the mouse...
  52. pmc Early restriction of peripheral and proximal cell lineages during formation of the lung
    Anne Karina T Perl
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, OH 45229 3039, USA
    Proc Natl Acad Sci U S A 99:10482-7. 2002
    ....
  53. ncbi request reprint Epithelial-vascular cross talk mediated by VEGF-A and HGF signaling directs primary septae formation during distal lung morphogenesis
    Hiroaki Yamamoto
    Department of Medicine and Lung Biology Center, University of California, San Francisco, CA 94143, USA
    Dev Biol 308:44-53. 2007
    ....
  54. pmc SPDEF regulates goblet cell hyperplasia in the airway epithelium
    Kwon Sik Park
    Division of Pulmonary Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    J Clin Invest 117:978-88. 2007
    ..SPDEF was induced following intratracheal allergen exposure and after Th2 cytokine stimulation and was sufficient to cause goblet cell differentiation of Clara cells in vivo...
  55. ncbi request reprint Surfactant protein B inhibits endotoxin-induced lung inflammation
    Ralph Epaud
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Am J Respir Cell Mol Biol 28:373-8. 2003
    ....
  56. ncbi request reprint Interleukin-1beta causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung
    Urpo Lappalainen
    Goteborg University, Department of Pediatrics, The Queen Silvia Children s Hospital, 41685 Goteborg, Sweden
    Am J Respir Cell Mol Biol 32:311-8. 2005
    ..Chronic production of IL-1beta in respiratory epithelial cells of adult mice causes lung inflammation, enlargement of distal airspaces, mucus metaplasia, and airway fibrosis in the adult mouse...
  57. ncbi request reprint Compensatory roles of Foxa1 and Foxa2 during lung morphogenesis
    Huajing Wan
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 280:13809-16. 2005
    ..Foxa family members regulate signaling and transcriptional programs required for morphogenesis and cell differentiation during formation of the lung...
  58. pmc Foxa2 is required for transition to air breathing at birth
    Huajing Wan
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Proc Natl Acad Sci U S A 101:14449-54. 2004
    ..Foxa2 regulates a complex pulmonary program of epithelial cell maturation required for transition to air breathing at birth...
  59. ncbi request reprint Temporal effects of Sprouty on lung morphogenesis
    Anne Karina T Perl
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Dev Biol 258:154-68. 2003
    ..These findings demonstrate that the embryonic-pseudoglandular stage is a critical time period during which Spry-sensitive pathways are required for branching morphogenesis, lobulation, and formation of the peripheral lung parenchyma...
  60. ncbi request reprint Role of Sonic hedgehog in patterning of tracheal-bronchial cartilage and the peripheral lung
    Leigh Anne D Miller
    Department of Pediatrics, Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA
    Dev Dyn 231:57-71. 2004
    ..SHH regulates branching morphogenesis and influences differentiation of the peripheral lung mesenchyme required for formation of bronchial and vascular smooth muscle...
  61. ncbi request reprint Midkine is regulated by hypoxia and causes pulmonary vascular remodeling
    Paul R Reynolds
    Divisions of Pulmonary Biology and Human Genetics, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    J Biol Chem 279:37124-32. 2004
    ..These data provide a model wherein the respiratory epithelium responds to hypoxia via HIF-1alpha-dependent regulation of MK, enhancing myocardin expression to influence pulmonary vascular gene expression...
  62. ncbi request reprint TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression
    Mario DeFelice
    Stazione Zoologica A Dohrn, Villa Comunale, 80121 Naples, Italy
    J Biol Chem 278:35574-83. 2003
    ..Direct and indirect transcriptional targets of TTF-1 were identified that are likely to play important roles in lung formation and function...
  63. ncbi request reprint Inhibitory effects of surfactant protein A on surfactant phospholipid hydrolysis by secreted phospholipases A2
    Sophie Chabot
    Unité de Défense Innée et Inflammation, Institut Pasteur, Institut National de la Santé et de la Recherche Médicale E 336, Paris, France
    J Immunol 171:995-1000. 2003
    ..We conclude that SP-A inhibits sPLA(2) activity, which may play a protective role by maintaining surfactant integrity during lung injury...
  64. pmc Stat-3 is required for pulmonary homeostasis during hyperoxia
    Isamu Hokuto
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Clin Invest 113:28-37. 2004
    ..Expression of Stat-3 in respiratory epithelial cells is not required for lung formation, but plays a critical role in maintenance of surfactant homeostasis and lung function during oxygen injury...
  65. ncbi request reprint Foxf1 haploinsufficiency reduces Notch-2 signaling during mouse lung development
    Vladimir V Kalinichenko
    Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois, Chicago, IL 60607 7170, USA
    Am J Physiol Lung Cell Mol Physiol 286:L521-30. 2004
    ..Foxf1 haploinsufficiency disrupted pulmonary expression of genes in the Notch-2-signaling pathway and resulted in abnormal development of lung microvasculature...
  66. ncbi request reprint Inhibition of alveolarization and altered pulmonary mechanics in mice expressing GATA-6
    Cong Liu
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 285:L1246-54. 2003
    ..Normal postnatal morphogenesis of the lung depends upon precise temporal-spatial regulation of GATA-6...
  67. ncbi request reprint Surfactant protein-D regulates soluble CD14 through matrix metalloproteinase-12
    Albert P Senft
    Division of Neonatology and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    J Immunol 174:4953-9. 2005
    ..Supporting this observation, MMP-12 caused the release of sCD14 from RAW 264.7 cells in vitro. In conclusion, SP-D influences innate host defense, in part, by regulating sCD14 in a process mediated by MMP-9 and MMP-12...
  68. ncbi request reprint Wnt/beta-catenin signaling acts upstream of N-myc, BMP4, and FGF signaling to regulate proximal-distal patterning in the lung
    Weiguo Shu
    Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Biol 283:226-39. 2005
    ..Thus, Wnt/beta-catenin signaling is a critical upstream regulator of proximal-distal patterning in the lung, in part, through regulation of N-myc, BMP4, and FGF signaling...
  69. ncbi request reprint Thyroid transcription factor (TTF) -1 regulates the expression of midkine (MK) during lung morphogenesis
    Paul R Reynolds
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229 3039, USA
    Dev Dyn 227:227-37. 2003
    ..TTF-1 regulates expression of MK in the lung. The temporal/spatial distribution of midkine is consistent with a potential role in paracrine signaling during lung morphogenesis...
  70. ncbi request reprint In vivo measurements of changes in respiratory mechanics with age in mice deficient in surfactant protein D
    Rachel A Collins
    Division of Clinical Sciences, Telethon Institute for Child Health Research, Perth, Western Australia
    Pediatr Res 53:463-7. 2003
    ..These data demonstrate that the changes in lung structure in SP-D (-/-) mice are reflected in the mechanical properties of both airway and lung parenchyma measured in vivo...
  71. ncbi request reprint Foxa2 regulates alveolarization and goblet cell hyperplasia
    Huajing Wan
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 131:953-64. 2004
    ..FOXA2 is required for alveolarization and regulates airway epithelial cell differentiation in the postnatal lung...
  72. ncbi request reprint Correction of pulmonary abnormalities in Sftpd-/- mice requires the collagenous domain of surfactant protein D
    Paul S Kingma
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 281:24496-505. 2006
    ..The collagen domain of SP-D is required for the regulation of pulmonary macrophage activation, airspace remodeling, and surfactant lipid homeostasis...
  73. ncbi request reprint Stat3 is required for cytoprotection of the respiratory epithelium during adenoviral infection
    Yohei Matsuzaki
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    J Immunol 177:527-37. 2006
    ..Thus, Stat3 plays a critical cytoprotective role that is required for epithelial cell survival and maintenance of alveolar structures during the early phases of pulmonary adenoviral infection...
  74. ncbi request reprint Sox17 influences the differentiation of respiratory epithelial cells
    Kwon Sik Park
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Dev Biol 294:192-202. 2006
    ..Sites of expression and the effects of Sox17 in vivo and in vitro are consistent with a role for Sox17 or other members of the Sox family of transcription factors in differentiation of the conducting airway epithelium...
  75. ncbi request reprint Neither SP-A nor NH2-terminal domains of SP-A can substitute for SP-D in regulation of alveolar homeostasis
    Liqian Zhang
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, OH 45229, USA
    Am J Physiol Lung Cell Mol Physiol 291:L181-90. 2006
    ....
  76. ncbi request reprint C/EBPalpha is required for lung maturation at birth
    Prithy C Martis
    Division of Pulmonary Biology and Neonatology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Development 133:1155-64. 2006
    ..C/EBPalpha participates in a transcriptional network that is required for the regulation of genes mediating perinatal lung maturation and surfactant homeostasis that is necessary for adaptation to air breathing at birth...
  77. ncbi request reprint PARP-2 interacts with TTF-1 and regulates expression of surfactant protein-B
    Yutaka Maeda
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 281:9600-6. 2006
    ..PARP-2 and PARP-1 interact with TTF-1 and regulate the expression of surfactant protein B, a protein required for lung function...
  78. pmc Transdifferentiation of ciliated cells during repair of the respiratory epithelium
    Kwon Sik Park
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 34:151-7. 2006
    ..Taken together, these findings demonstrate that ciliated epithelial cells spread and transdifferentiate into distinct epithelial cell types to repair the airway epithelium...
  79. ncbi request reprint SP-B deficiency causes respiratory failure in adult mice
    Kristin R Melton
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 285:L543-9. 2003
    ..Reduction of alveolar SP-B content causes surfactant dysfunction and respiratory failure, indicating that SP-B is required for postnatal lung function...
  80. pmc Identification of the surfactant protein A receptor 210 as the unconventional myosin 18A
    Ching Hui Yang
    Center of Biomedical Research, University of Texas Health Center, Tyler, Texas 75708 3154, USA
    J Biol Chem 280:34447-57. 2005
    ..Antibodies raised against the neck domain of MyoXVIIIA blocked the binding of SP-A to macrophages. Together, these findings indicate that MyoXVIIIA constitutes a novel receptor for SP-A...
  81. ncbi request reprint Disrupted pulmonary vascular development and pulmonary hypertension in transgenic mice overexpressing transforming growth factor-alpha
    Timothy D Le Cras
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Am J Physiol Lung Cell Mol Physiol 285:L1046-54. 2003
    ..Reductions in VEGF may contribute to the pathogenesis of pulmonary vascular disease in TGF-alpha mice...

Research Grants30

  1. PULMONARY AND CARDIOVASCULAR DEVELOPMENT TRAINING GRANT
    Jeffrey Whitsett; Fiscal Year: 2007
    ..Regular research meetings and several pertinent seminar series further enhance the program. Progress of each trainee will be reviewed annually by the Executive Committee. ..
  2. ABCA3 and Alveolar Homeostasis
    Jeffrey Whitsett; Fiscal Year: 2007
    ....
  3. Transcriptional Control of Respiratory Epithelial Progenitor Cells
    Jeffrey Whitsett; Fiscal Year: 2007
    ..A Stem Cell Consortium will be established at CCHMC, Univ. of Cincinnati College of Medicine to develop education, training, and research bridging hematopoietic stem cell and pulmonary stem cell biology on our campus. ..
  4. Transcriptional Programming of Asthma Related Pathology in Respiratory Epithelia
    Jeffrey Whitsett; Fiscal Year: 2009
    ..The work will identify mechanisms regulating airway epithelial homeostasis that will provide novel targets to diagnose and influence the pathogenesis of chronic pulmonary diseases affecting the airways. ..
  5. ABCA3 and Alveolar Homeostasis
    Jeffrey Whitsett; Fiscal Year: 2009
    ....
  6. Transcriptional Control of Respiratory Epithelial Progenitor Cells
    Jeffrey Whitsett; Fiscal Year: 2009
    ..A Stem Cell Consortium will be established at CCHMC, Univ. of Cincinnati College of Medicine to develop education, training, and research bridging hematopoietic stem cell and pulmonary stem cell biology on our campus. ..
  7. Transcriptional Programming of Asthma Related Pathology in Respiratory Epithelia
    Jeffrey A Whitsett; Fiscal Year: 2010
    ..The work will identify mechanisms regulating airway epithelial homeostasis that will provide novel targets to diagnose and influence the pathogenesis of chronic pulmonary diseases affecting the airways. ..
  8. ABCA3 and Alveolar Homeostasis
    Jeffrey A Whitsett; Fiscal Year: 2010
    ....
  9. Transcriptional Control of Respiratory Epithelial Progenitor Cells
    Jeffrey Whitsett; Fiscal Year: 2009
    ..A Stem Cell Consortium will be established at CCHMC, Univ. of Cincinnati College of Medicine to develop education, training, and research bridging hematopoietic stem cell and pulmonary stem cell biology on our campus. ..
  10. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE SP-B
    Jeffrey Whitsett; Fiscal Year: 2007
    ..Transcriptional responses to decreased SP-B will be determined using RNA microarray, to identify the molecular pathways mediating or compensating for reduced SP-B/surfactant function in vivo. ..
  11. ABCA3 and Alveolar Homeostasis
    Jeffrey Whitsett; Fiscal Year: 2006
    ....
  12. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE
    Jeffrey Whitsett; Fiscal Year: 1990
    ..Knowledge of the role of SAP-6-Phe in surfactant biology and control of SAP-6-Phe synthesis will be important in developing new strategies for therapy of hyaline membrane disease in newborn infants...
  13. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE
    Jeffrey Whitsett; Fiscal Year: 1991
    ..Knowledge of the role of SAP-6-Phe in surfactant biology and control of SAP-6-Phe synthesis will be important in developing new strategies for therapy of hyaline membrane disease in newborn infants...
  14. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE SP-B
    Jeffrey Whitsett; Fiscal Year: 1993
    ..Further understanding of the role of SP-B in lung function and the mechanisms regulating SP-B may provide insights critical to developing new therapeutic strategies for prevention of lung disease in premature infants...
  15. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE SP-B
    Jeffrey Whitsett; Fiscal Year: 2003
    ..Transcriptional responses to decreased SP-B will be determined using RNA microarray, to identify the molecular pathways mediating or compensating for reduced SP-B/surfactant function in vivo. ..
  16. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE SP-B
    Jeffrey Whitsett; Fiscal Year: 2004
    ..Transcriptional responses to decreased SP-B will be determined using RNA microarray, to identify the molecular pathways mediating or compensating for reduced SP-B/surfactant function in vivo. ..
  17. SHH and Alveolar Formation
    Jeffrey Whitsett; Fiscal Year: 2003
    ..abstract_text> ..
  18. SHH and Alveolar Formation
    Jeffrey Whitsett; Fiscal Year: 2004
    ..abstract_text> ..
  19. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE SP-B
    Jeffrey Whitsett; Fiscal Year: 2005
    ..Transcriptional responses to decreased SP-B will be determined using RNA microarray, to identify the molecular pathways mediating or compensating for reduced SP-B/surfactant function in vivo. ..
  20. SHH and Alveolar Formation
    Jeffrey Whitsett; Fiscal Year: 2005
    ..abstract_text> ..
  21. PERINATAL EXPRESSION OF SURFACTANT PROTEIN GENE SP-B
    Jeffrey Whitsett; Fiscal Year: 2006
    ..Transcriptional responses to decreased SP-B will be determined using RNA microarray, to identify the molecular pathways mediating or compensating for reduced SP-B/surfactant function in vivo. ..
  22. SHH and Alveolar Formation
    Jeffrey Whitsett; Fiscal Year: 2006
    ..abstract_text> ..
  23. Transcriptional Control of Respiratory Epithelial Progenitor Cells
    Jeffrey A Whitsett; Fiscal Year: 2010
    ..A Stem Cell Consortium will be established at CCHMC, Univ. of Cincinnati College of Medicine to develop education, training, and research bridging hematopoietic stem cell and pulmonary stem cell biology on our campus. ..