Dao Pan

Summary

Affiliation: Cincinnati Children's Hospital Medical Center
Country: USA

Publications

  1. ncbi request reprint Cell- and gene-based therapeutic approaches for neurological deficits in mucopolysaccharidoses
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, OH 45229, USA
    Curr Pharm Biotechnol 12:884-96. 2011
  2. pmc Progression of multiple behavioral deficits with various ages of onset in a murine model of Hurler syndrome
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, OH 45249, USA
    Brain Res 1188:241-53. 2008
  3. ncbi request reprint Improved gene transfer and normalized enzyme levels in primitive hematopoietic progenitors from patients with mucopolysaccharidosis type I using a bioreactor
    Dao Pan
    Gene Therapy Program, Department of Pediatrics, and Institute of Human Genetics, University of Minnesota, Minneapolis, MN, USA
    J Gene Med 6:1293-303. 2004
  4. ncbi request reprint Correction of metabolic, craniofacial, and neurologic abnormalities in MPS I mice treated at birth with adeno-associated virus vector transducing the human alpha-L-iduronidase gene
    Seth D Hartung
    Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 9:866-75. 2004
  5. pmc Reprogramming erythroid cells for lysosomal enzyme production leads to visceral and CNS cross-correction in mice with Hurler syndrome
    Daren Wang
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 106:19958-63. 2009
  6. pmc In vivo gene transfer into adult stem cells in unconditioned mice by in situ delivery of a lentiviral vector
    D Nicole Worsham
    Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45249, USA
    Mol Ther 14:514-24. 2006
  7. ncbi request reprint Craniofacial abnormalities in a murine knock-out model of mucopolysaccharidosis I H: a computed tomography and anatomic study
    Patrick Graupman
    Department of Neurosurgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Craniofac Surg 15:392-8. 2004
  8. ncbi request reprint Co-expression of MGMT(P140K) and alpha-L-iduronidase in primary hepatocytes from mucopolysaccharidosis type I mice enables efficient selection with metabolic correction
    Daren Wang
    Cell and Molecular Therapy Program, and Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    J Gene Med 10:249-59. 2008
  9. pmc Engineering a lysosomal enzyme with a derivative of receptor-binding domain of apoE enables delivery across the blood-brain barrier
    Daren Wang
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 110:2999-3004. 2013
  10. pmc K-Cl cotransporter gene expression during human and murine erythroid differentiation
    Dao Pan
    Molecular and Cell Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 286:30492-503. 2011

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Cell- and gene-based therapeutic approaches for neurological deficits in mucopolysaccharidoses
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, OH 45229, USA
    Curr Pharm Biotechnol 12:884-96. 2011
    ..Several neurological findings in CNS pathophysiology emerged with therapeutic investigation will also be discussed...
  2. pmc Progression of multiple behavioral deficits with various ages of onset in a murine model of Hurler syndrome
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, OH 45249, USA
    Brain Res 1188:241-53. 2008
    ..This study would also provide guidelines for the experimental designs of behavioral evaluation on innovative therapies for the treatment of MPS type I...
  3. ncbi request reprint Improved gene transfer and normalized enzyme levels in primitive hematopoietic progenitors from patients with mucopolysaccharidosis type I using a bioreactor
    Dao Pan
    Gene Therapy Program, Department of Pediatrics, and Institute of Human Genetics, University of Minnesota, Minneapolis, MN, USA
    J Gene Med 6:1293-303. 2004
    ..Other inadequacies of current transduction protocols are related to their multi-step procedures, e.g., using tissue-culture flasks, roller bottles or gas-permeable bags for clinical application...
  4. ncbi request reprint Correction of metabolic, craniofacial, and neurologic abnormalities in MPS I mice treated at birth with adeno-associated virus vector transducing the human alpha-L-iduronidase gene
    Seth D Hartung
    Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 9:866-75. 2004
    ..We conclude that AAV-mediated transduction of the IDUA gene in newborn Idua(-/-) mice was sufficient to have a major curative impact on several of the most important parameters of the disease...
  5. pmc Reprogramming erythroid cells for lysosomal enzyme production leads to visceral and CNS cross-correction in mice with Hurler syndrome
    Daren Wang
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 106:19958-63. 2009
    ..This approach provides a paradigm for the utilization of RBC precursors as a depot for efficient and potentially safer systemic delivery of nonsecreted proteins by ex vivo HSC gene transfer...
  6. pmc In vivo gene transfer into adult stem cells in unconditioned mice by in situ delivery of a lentiviral vector
    D Nicole Worsham
    Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45249, USA
    Mol Ther 14:514-24. 2006
    ..This approach could lead to a novel application for treatment of human diseases...
  7. ncbi request reprint Craniofacial abnormalities in a murine knock-out model of mucopolysaccharidosis I H: a computed tomography and anatomic study
    Patrick Graupman
    Department of Neurosurgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Craniofac Surg 15:392-8. 2004
    ..This information now provides researchers with objective data from living Hurler syndrome-affected mice that will allow them to follow therapies directed at improving craniofacial outcomes for any therapy over time...
  8. ncbi request reprint Co-expression of MGMT(P140K) and alpha-L-iduronidase in primary hepatocytes from mucopolysaccharidosis type I mice enables efficient selection with metabolic correction
    Daren Wang
    Cell and Molecular Therapy Program, and Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    J Gene Med 10:249-59. 2008
    ..It has been shown that an O(6)-methylguanine-DNA-methyltransferase variant (MGMT(P140K)) mediated in vivo selection of transduced hematopoietic stem cells (HSC) in animals...
  9. pmc Engineering a lysosomal enzyme with a derivative of receptor-binding domain of apoE enables delivery across the blood-brain barrier
    Daren Wang
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 110:2999-3004. 2013
    ..These findings provide a noninvasive and BBB-targeted procedure for the delivery of large-molecule therapeutic agents to treat neurological lysosomal storage disorders and potentially other diseases that involve the brain...
  10. pmc K-Cl cotransporter gene expression during human and murine erythroid differentiation
    Dao Pan
    Molecular and Cell Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 286:30492-503. 2011
    ..The results suggest that KCC3 is the dominant isoform in erythrocytes, with variable expression of KCC1 and KCC4 among individuals that could result in modulation of KCC activity...
  11. doi request reprint Secreted luciferase for in vivo evaluation of systemic protein delivery in mice
    Salim S El-Amouri
    Molecular and Cell Therapy Program, Division of Experimental Hematology and Cancer Biology, Cancer and Blood Diseases Institute, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    Mol Biotechnol 53:63-73. 2013
    ..These results suggest that Gluc-based system may provide a useful tool for in vivo evaluation of protein/agent biodistribution following systemic delivery...
  12. doi request reprint In situ (in vivo) gene transfer into murine bone marrow stem cells
    Dao Pan
    Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, OH, USA
    Methods Mol Biol 506:159-69. 2009
    ..This approach may provide a novel application for treatment of human diseases, and represent an interesting new tool to study adult stem cell plasticity and the nature of unperturbed hematopoiesis...
  13. ncbi request reprint Biodistribution and toxicity studies of VSVG-pseudotyped lentiviral vector after intravenous administration in mice with the observation of in vivo transduction of bone marrow
    Dao Pan
    Department of Pediatrics and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 6:19-29. 2002
    ..The observation of bone marrow transduction after intravenous vector administration suggests the possibility of an in vivo approach to stem cell gene therapy...
  14. pmc Rac1 and Rac2 GTPases are necessary for early erythropoietic expansion in the bone marrow but not in the spleen
    Theodosia A Kalfa
    Division of Hematology Oncology, Oncology, Cincinnati Children s Research Foundation, Cincinnati Children s Hospital Medical Center, MLC 7015 Cincinnati, OH 45229 3039, USA
    Haematologica 95:27-35. 2010
    ..The role of these Rac GTPases in erythropoiesis has not yet been fully elucidated...

Research Grants5

  1. Genetic Therapy for CNS Manifestations in MPS I via BBB-targeted Protein Delivery
    Dao Pan; Fiscal Year: 2009
    ..abstract_text> ..
  2. Genetic Therapy for CNS Manifestations in MPS I via BBB-targeted Protein Delivery
    Dao Pan; Fiscal Year: 2010
    ..The studies described in this application will open the door to novel approaches for the treatment of neurological disorders-from MPS type I to major public health concerns such as stroke and Alzheimer's disease. ..
  3. In Vivo BM Stem Cell Gene Transfer for MPS type I
    Dao Pan; Fiscal Year: 2006
    ..Taken together, these data would not only open a door to a novel approach for treatment of human diseases, but also provide a new tool to study adult stem cell plasticity and the nature of hematopoiesis. ..