Dao Pan

Summary

Affiliation: Cincinnati Children's Hospital Medical Center
Country: USA

Publications

  1. ncbi Improved gene transfer and normalized enzyme levels in primitive hematopoietic progenitors from patients with mucopolysaccharidosis type I using a bioreactor
    Dao Pan
    Gene Therapy Program, Department of Pediatrics, and Institute of Human Genetics, University of Minnesota, Minneapolis, MN, USA
    J Gene Med 6:1293-303. 2004
  2. ncbi Progression of multiple behavioral deficits with various ages of onset in a murine model of Hurler syndrome
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, OH 45249, USA
    Brain Res 1188:241-53. 2008
  3. ncbi Cell- and gene-based therapeutic approaches for neurological deficits in mucopolysaccharidoses
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, OH 45229, USA
    Curr Pharm Biotechnol 12:884-96. 2011
  4. ncbi Correction of metabolic, craniofacial, and neurologic abnormalities in MPS I mice treated at birth with adeno-associated virus vector transducing the human alpha-L-iduronidase gene
    Seth D Hartung
    Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 9:866-75. 2004
  5. ncbi Reprogramming erythroid cells for lysosomal enzyme production leads to visceral and CNS cross-correction in mice with Hurler syndrome
    Daren Wang
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 106:19958-63. 2009
  6. ncbi In vivo gene transfer into adult stem cells in unconditioned mice by in situ delivery of a lentiviral vector
    D Nicole Worsham
    Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45249, USA
    Mol Ther 14:514-24. 2006
  7. ncbi Co-expression of MGMT(P140K) and alpha-L-iduronidase in primary hepatocytes from mucopolysaccharidosis type I mice enables efficient selection with metabolic correction
    Daren Wang
    Cell and Molecular Therapy Program, and Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    J Gene Med 10:249-59. 2008
  8. ncbi Craniofacial abnormalities in a murine knock-out model of mucopolysaccharidosis I H: a computed tomography and anatomic study
    Patrick Graupman
    Department of Neurosurgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Craniofac Surg 15:392-8. 2004
  9. ncbi K-Cl cotransporter gene expression during human and murine erythroid differentiation
    Dao Pan
    Molecular and Cell Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 286:30492-503. 2011
  10. ncbi In situ (in vivo) gene transfer into murine bone marrow stem cells
    Dao Pan
    Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, OH, USA
    Methods Mol Biol 506:159-69. 2009

Research Grants

  1. Genetic Therapy for CNS Manifestations in MPS I via BBB-targeted Protein Delivery
    Dao Pan; Fiscal Year: 2009
  2. Genetic Therapy for CNS Manifestations in MPS I via BBB-targeted Protein Delivery
    Dao Pan; Fiscal Year: 2010
  3. In Vivo BM Stem Cell Gene Transfer for MPS type I
    Dao Pan; Fiscal Year: 2006

Collaborators

Detail Information

Publications12

  1. ncbi Improved gene transfer and normalized enzyme levels in primitive hematopoietic progenitors from patients with mucopolysaccharidosis type I using a bioreactor
    Dao Pan
    Gene Therapy Program, Department of Pediatrics, and Institute of Human Genetics, University of Minnesota, Minneapolis, MN, USA
    J Gene Med 6:1293-303. 2004
    ..Other inadequacies of current transduction protocols are related to their multi-step procedures, e.g., using tissue-culture flasks, roller bottles or gas-permeable bags for clinical application...
  2. ncbi Progression of multiple behavioral deficits with various ages of onset in a murine model of Hurler syndrome
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, OH 45249, USA
    Brain Res 1188:241-53. 2008
    ..This study would also provide guidelines for the experimental designs of behavioral evaluation on innovative therapies for the treatment of MPS type I...
  3. ncbi Cell- and gene-based therapeutic approaches for neurological deficits in mucopolysaccharidoses
    Dao Pan
    Molecular and Gene Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, OH 45229, USA
    Curr Pharm Biotechnol 12:884-96. 2011
    ..Several neurological findings in CNS pathophysiology emerged with therapeutic investigation will also be discussed...
  4. ncbi Correction of metabolic, craniofacial, and neurologic abnormalities in MPS I mice treated at birth with adeno-associated virus vector transducing the human alpha-L-iduronidase gene
    Seth D Hartung
    Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 9:866-75. 2004
    ..We conclude that AAV-mediated transduction of the IDUA gene in newborn Idua(-/-) mice was sufficient to have a major curative impact on several of the most important parameters of the disease...
  5. ncbi Reprogramming erythroid cells for lysosomal enzyme production leads to visceral and CNS cross-correction in mice with Hurler syndrome
    Daren Wang
    Division of Experimental Hematology and Cancer Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 106:19958-63. 2009
    ..This approach provides a paradigm for the utilization of RBC precursors as a depot for efficient and potentially safer systemic delivery of nonsecreted proteins by ex vivo HSC gene transfer...
  6. ncbi In vivo gene transfer into adult stem cells in unconditioned mice by in situ delivery of a lentiviral vector
    D Nicole Worsham
    Division of Experimental Hematology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45249, USA
    Mol Ther 14:514-24. 2006
    ..This approach could lead to a novel application for treatment of human diseases...
  7. ncbi Co-expression of MGMT(P140K) and alpha-L-iduronidase in primary hepatocytes from mucopolysaccharidosis type I mice enables efficient selection with metabolic correction
    Daren Wang
    Cell and Molecular Therapy Program, and Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    J Gene Med 10:249-59. 2008
    ..It has been shown that an O(6)-methylguanine-DNA-methyltransferase variant (MGMT(P140K)) mediated in vivo selection of transduced hematopoietic stem cells (HSC) in animals...
  8. ncbi Craniofacial abnormalities in a murine knock-out model of mucopolysaccharidosis I H: a computed tomography and anatomic study
    Patrick Graupman
    Department of Neurosurgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Craniofac Surg 15:392-8. 2004
    ..This information now provides researchers with objective data from living Hurler syndrome-affected mice that will allow them to follow therapies directed at improving craniofacial outcomes for any therapy over time...
  9. ncbi K-Cl cotransporter gene expression during human and murine erythroid differentiation
    Dao Pan
    Molecular and Cell Therapy Program, Division of Experimental Hematology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 286:30492-503. 2011
    ..The results suggest that KCC3 is the dominant isoform in erythrocytes, with variable expression of KCC1 and KCC4 among individuals that could result in modulation of KCC activity...
  10. ncbi In situ (in vivo) gene transfer into murine bone marrow stem cells
    Dao Pan
    Division of Experimental Hematology, Cincinnati Children s Research Foundation, Cincinnati, OH, USA
    Methods Mol Biol 506:159-69. 2009
    ..This approach may provide a novel application for treatment of human diseases, and represent an interesting new tool to study adult stem cell plasticity and the nature of unperturbed hematopoiesis...
  11. ncbi Biodistribution and toxicity studies of VSVG-pseudotyped lentiviral vector after intravenous administration in mice with the observation of in vivo transduction of bone marrow
    Dao Pan
    Department of Pediatrics and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Mol Ther 6:19-29. 2002
    ..The observation of bone marrow transduction after intravenous vector administration suggests the possibility of an in vivo approach to stem cell gene therapy...
  12. ncbi Rac1 and Rac2 GTPases are necessary for early erythropoietic expansion in the bone marrow but not in the spleen
    Theodosia A Kalfa
    Division of Hematology Oncology, Oncology, Cincinnati Children s Research Foundation, Cincinnati Children s Hospital Medical Center, MLC 7015 Cincinnati, OH 45229 3039, USA
    Haematologica 95:27-35. 2010
    ..The role of these Rac GTPases in erythropoiesis has not yet been fully elucidated...

Research Grants5

  1. Genetic Therapy for CNS Manifestations in MPS I via BBB-targeted Protein Delivery
    Dao Pan; Fiscal Year: 2009
    ..abstract_text> ..
  2. Genetic Therapy for CNS Manifestations in MPS I via BBB-targeted Protein Delivery
    Dao Pan; Fiscal Year: 2010
    ..The studies described in this application will open the door to novel approaches for the treatment of neurological disorders-from MPS type I to major public health concerns such as stroke and Alzheimer's disease. ..
  3. In Vivo BM Stem Cell Gene Transfer for MPS type I
    Dao Pan; Fiscal Year: 2006
    ..Taken together, these data would not only open a door to a novel approach for treatment of human diseases, but also provide a new tool to study adult stem cell plasticity and the nature of hematopoiesis. ..