Machiko Ikegami

Summary

Affiliation: Cincinnati Children's Hospital Medical Center
Country: USA

Publications

  1. pmc CCAAT/enhancer-binding protein-α suppresses lung tumor development in mice through the p38α MAP kinase pathway
    Atsuyasu Sato
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, OH, USA
    PLoS ONE 8:e57013. 2013
  2. pmc SP-B and SP-C containing new synthetic surfactant for treatment of extremely immature lamb lung
    Atsuyasu Sato
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio, United States of America
    PLoS ONE 7:e39392. 2012
  3. ncbi request reprint Minimal lung and systemic responses to TNF-alpha in preterm sheep
    Machiko Ikegami
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 285:L121-9. 2003
  4. pmc Pulmonary surfactant surface tension influences alveolar capillary shape and oxygenation
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 41:433-9. 2009
  5. ncbi request reprint STAT-3 regulates surfactant phospholipid homeostasis in normal lung and during endotoxin-mediated lung injury
    Machiko Ikegami
    Cincinnati Children s Hospital, Div of Pulmonary Biology, Cincinnati, OH 45229 3039, USA
    J Appl Physiol (1985) 104:1753-60. 2008
  6. ncbi request reprint Alveolar lipoproteinosis in an acid sphingomyelinase-deficient mouse model of Niemann-Pick disease
    Machiko Ikegami
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229, USA
    Am J Physiol Lung Cell Mol Physiol 284:L518-25. 2003
  7. pmc Intratracheal recombinant surfactant protein d prevents endotoxin shock in the newborn preterm lamb
    Machiko Ikegami
    Cincinnati Children s Hospital, Division of Pulmonary Biology, 3333 Burnet Avenue, University of Cincinnati, Ohio 45229 3039, USA
    Am J Respir Crit Care Med 173:1342-7. 2006
  8. doi request reprint Surfactant protein-D regulates the postnatal maturation of pulmonary surfactant lipid pool sizes
    Machiko Ikegami
    Div of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, ML 7029, 3333 Burnet Ave, Cincinnati, Ohio 45229 3039, USA
    J Appl Physiol (1985) 106:1545-52. 2009
  9. ncbi request reprint Initial responses to ventilation of premature lambs exposed to intra-amniotic endotoxin 4 days before delivery
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 286:L573-9. 2004
  10. ncbi request reprint Reversibility of lung inflammation caused by SP-B deficiency
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 289:L962-70. 2005

Detail Information

Publications89

  1. pmc CCAAT/enhancer-binding protein-α suppresses lung tumor development in mice through the p38α MAP kinase pathway
    Atsuyasu Sato
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, OH, USA
    PLoS ONE 8:e57013. 2013
    ..In conclusion, the role of C/EBPα as a lung tumor suppressor was demonstrated for the first time in the present study, and the extinguished C/EBPα expression through p38α inactivation leads tumor promotion and progression...
  2. pmc SP-B and SP-C containing new synthetic surfactant for treatment of extremely immature lamb lung
    Atsuyasu Sato
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio, United States of America
    PLoS ONE 7:e39392. 2012
    ..In conclusion, the new synthetic surfactant CHF5633 was effective in treating extremely immature newborn lambs with surfactant deficiency during the 5 h study period...
  3. ncbi request reprint Minimal lung and systemic responses to TNF-alpha in preterm sheep
    Machiko Ikegami
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 285:L121-9. 2003
    ..Therefore, the presence of a mediator such as TNF-alpha in a developing animal does not necessarily mean that it is causing the responses anticipated from previous results in adult animals...
  4. pmc Pulmonary surfactant surface tension influences alveolar capillary shape and oxygenation
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 41:433-9. 2009
    ....
  5. ncbi request reprint STAT-3 regulates surfactant phospholipid homeostasis in normal lung and during endotoxin-mediated lung injury
    Machiko Ikegami
    Cincinnati Children s Hospital, Div of Pulmonary Biology, Cincinnati, OH 45229 3039, USA
    J Appl Physiol (1985) 104:1753-60. 2008
    ..STAT-3 plays a critical role in the regulation of surfactant lipid synthesis in the normal lung and during lung injury caused by LPS...
  6. ncbi request reprint Alveolar lipoproteinosis in an acid sphingomyelinase-deficient mouse model of Niemann-Pick disease
    Machiko Ikegami
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229, USA
    Am J Physiol Lung Cell Mol Physiol 284:L518-25. 2003
    ..Alterations in surfactant composition, including increased sphingomyelin content, contributed to the abnormal surfactant function observed in the ASM-deficient mouse...
  7. pmc Intratracheal recombinant surfactant protein d prevents endotoxin shock in the newborn preterm lamb
    Machiko Ikegami
    Cincinnati Children s Hospital, Division of Pulmonary Biology, 3333 Burnet Avenue, University of Cincinnati, Ohio 45229 3039, USA
    Am J Respir Crit Care Med 173:1342-7. 2006
    ..Surfactant protein (SP) D is a member of the collectin family of innate immune molecules that plays an important role in innate host defense of the lung...
  8. doi request reprint Surfactant protein-D regulates the postnatal maturation of pulmonary surfactant lipid pool sizes
    Machiko Ikegami
    Div of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, ML 7029, 3333 Burnet Ave, Cincinnati, Ohio 45229 3039, USA
    J Appl Physiol (1985) 106:1545-52. 2009
    ..SP-D regulates the dramatic decreases in the surfactant pool size that occurs in the newborn period...
  9. ncbi request reprint Initial responses to ventilation of premature lambs exposed to intra-amniotic endotoxin 4 days before delivery
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 286:L573-9. 2004
    ..After lung inflammation was induced by intra-amniotic endotoxin injection, ventilation did not increase lung injury...
  10. ncbi request reprint Reversibility of lung inflammation caused by SP-B deficiency
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 289:L962-70. 2005
    ..SP-B reversed inflammation and maintained lung function in vivo, indicating its potential utility for the prevention and treatment of pulmonary injury and surfactant deficiency...
  11. ncbi request reprint Surfactant catabolism
    Machiko Ikegami
    Cincinnati Children s Hospital, Division of Pulmonary Biology, University of Cincinnati, Cincinnati, OH 45229, USA
    Respirology 11:S24-7. 2006
    ..In this review the author will discuss: (i) the mechanisms of the surfactant catabolism by macrophage and type II cells; and (ii) the important role of SP-D on ultrastructure of surfactant that affects uptake by type II cells...
  12. ncbi request reprint Surfactant protein-D and surfactant inhibit endotoxin-induced pulmonary inflammation
    Machiko Ikegami
    Cincinnati Children s Hospital, Division of Pulmonary Biology, 3333 Burnet Ave, University of Cincinnati, OH 45229 3039, USA
    Chest 132:1447-54. 2007
    ..In this study, the effect of exogenous recombinant human SP-D (rhSP-D) on protection of the adult mouse lung from lipopolysaccharide (LPS)-induced and lipoteichoic acid (LTA)-induced injury was assessed...
  13. ncbi request reprint TGF-alpha perturbs surfactant homeostasis in vivo
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 289:L34-43. 2005
    ..Abnormalities in surfactant function and decreased surfactant level in the airways may contribute to the pathophysiology induced by TGF-alpha in both the developing and adult lung...
  14. ncbi request reprint Surfactant protein D influences surfactant ultrastructure and uptake by alveolar type II cells
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 288:L552-61. 2005
    ..SP-D regulates uptake and catabolism by type II cells and influences the ultrastructure of surfactant in the alveolus...
  15. pmc Foxa2 is required for transition to air breathing at birth
    Huajing Wan
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Proc Natl Acad Sci U S A 101:14449-54. 2004
    ..Foxa2 regulates a complex pulmonary program of epithelial cell maturation required for transition to air breathing at birth...
  16. pmc Rapamycin prevents transforming growth factor-alpha-induced pulmonary fibrosis
    Thomas R Korfhagen
    Department of Pulmonary Medicine, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Am J Respir Cell Mol Biol 41:562-72. 2009
    ....
  17. doi request reprint Kruppel-like factor 5 is required for perinatal lung morphogenesis and function
    Huajing Wan
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and the University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 135:2563-72. 2008
    ..KLF5 regulates genes controlling paracrine interactions during lung morphogenesis, as well as those regulating the maturation of the respiratory epithelium that is required for lung function after birth...
  18. ncbi request reprint Pneumonitis and emphysema in sp-C gene targeted mice
    Stephan W Glasser
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Ohio 45229 3039, USA
    J Biol Chem 278:14291-8. 2003
    ..Absence of SP-C caused a severe progressive pulmonary disorder with histologic features consistent with interstitial pneumonitis...
  19. pmc Transcriptional programs controlling perinatal lung maturation
    Yan Xu
    The Perinatal Institute and Section of Neonatology, Perinatal and Pulmonary Biology, University of Cincinnati, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    PLoS ONE 7:e37046. 2012
    ..The present study systemically mapped key regulators, bioprocesses, and transcriptional networks controlling lung maturation, providing the basis for new therapeutic strategies to enhance lung function in preterm infants...
  20. ncbi request reprint Neither SP-A nor NH2-terminal domains of SP-A can substitute for SP-D in regulation of alveolar homeostasis
    Liqian Zhang
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, OH 45229, USA
    Am J Physiol Lung Cell Mol Physiol 291:L181-90. 2006
    ....
  21. ncbi request reprint Maternal glucocorticoids increase endotoxin-induced lung inflammation in preterm lambs
    Suhas G Kallapur
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Ohio 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 284:L633-42. 2003
    ..These results have implications for lung inflammation and bronchopulmonary dysplasia in preterm infants exposed to chorioamnionitis and maternal glucocorticoids...
  22. pmc Calcineurin/Nfat signaling is required for perinatal lung maturation and function
    Vrushank Dave
    Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    J Clin Invest 116:2597-609. 2006
    ..The calcineurin/Nfat pathway controls the morphologic maturation of lungs prior to birth and regulates expression of genes involved in surfactant homeostasis that are critical for adaptation to air breathing...
  23. pmc IL-1 mediates pulmonary and systemic inflammatory responses to chorioamnionitis induced by lipopolysaccharide
    Suhas G Kallapur
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio, USA
    Am J Respir Crit Care Med 179:955-61. 2009
    ..However, chorioamnionitis is also associated with a paradoxical reduction in the incidence of surfactant deficiency-related respiratory distress syndrome in preterm infants...
  24. pmc C/EBP{alpha} is required for pulmonary cytoprotection during hyperoxia
    Yan Xu
    Cincinnati Children s Hospital Medical Center, University of Cincinnati, Ohio 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 297:L286-98. 2009
    ..While C/EBPalpha did not play a critical role in postnatal pulmonary function under normal conditions, C/EBPalpha mediated protection of the lung during acute lung injury induced by hyperoxia...
  25. pmc IL-8 signaling does not mediate intra-amniotic LPS-induced inflammation and maturation in preterm fetal lamb lung
    Suhas G Kallapur
    Cincinnati Children s Hospital Medical Center, University of Cincinnati, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 297:L512-9. 2009
    ..Inhibition of CXCR2 did not change IA LPS-induced lung maturation. IL-8 signaling does not mediate LPS-induced lung inflammation and lung maturation...
  26. ncbi request reprint The role of pulmonary collectin N-terminal domains in surfactant structure, function, and homeostasis in vivo
    Nades Palaniyar
    Division of Pulmonary Critical Care Medicine, Department of Medicine, Children s Hospital Research Foundation, University of Cincinnati School of Medicine, Cincinnati, OH 45267 0564, USA
    J Biol Chem 277:26971-9. 2002
    ....
  27. ncbi request reprint Surfactant protein B inhibits endotoxin-induced lung inflammation
    Ralph Epaud
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Am J Respir Cell Mol Biol 28:373-8. 2003
    ....
  28. pmc STAT3 regulates ABCA3 expression and influences lamellar body formation in alveolar type II cells
    Yohei Matsuzaki
    Cincinnati Children s Hospital Medical Center, Section of Neonatology, Perinatal and Pulmonary Biology, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 38:551-8. 2008
    ..ABCA3 expression is regulated by IL-6 in a pathway that includes STAT3, PI3K, AKT, SCAP, and SREBP. Activation of STAT3 after exposure to IL-6 enhances ABCA3 expression, which, in turn, influences pulmonary surfactant homeostasis...
  29. ncbi request reprint Correction of pulmonary abnormalities in Sftpd-/- mice requires the collagenous domain of surfactant protein D
    Paul S Kingma
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 281:24496-505. 2006
    ..The collagen domain of SP-D is required for the regulation of pulmonary macrophage activation, airspace remodeling, and surfactant lipid homeostasis...
  30. ncbi request reprint Reversibility of pulmonary abnormalities by conditional replacement of surfactant protein D (SP-D) in vivo
    Liqian Zhang
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 277:38709-13. 2002
    ..However, once established, emphysema was not reversed by SP-D. SP-D-dependent processes regulating surfactant lipid homeostasis were disassociated from those mediating emphysema...
  31. ncbi request reprint Injury responses to different surfactants in ventilated premature lamb lungs
    Machiko Ikegami
    Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, OH 45229 3039, USA
    Pediatr Res 51:689-95. 2002
    ....
  32. ncbi request reprint Perinatal increases in TGF-{alpha} disrupt the saccular phase of lung morphogenesis and cause remodeling: microarray analysis
    Elizabeth L Kramer
    Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    Am J Physiol Lung Cell Mol Physiol 293:L314-27. 2007
    ..Pathological changes in TGF-alpha-overexpressing mice bore similarities to premature infants born in the saccular phase who develop BPD, including remodeling of the distal lung septae and arteries...
  33. ncbi request reprint Chronic endotoxin exposure does not cause sustained structural abnormalities in the fetal sheep lungs
    Suhas G Kallapur
    Cincinnati Children s Hospital Medical Center, Div of Pulmonary Biology, University of Cincinnati College of Medicine, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 288:L966-74. 2005
    ..Contrary to our hypothesis, a prolonged fetal exposure to intra-amniotic endotoxin caused mild persistent inflammation but did not lead to progressive structural abnormalities in lungs of near-term gestation lambs...
  34. ncbi request reprint Deficiency of SP-B reveals protective role of SP-C during oxygen lung injury
    Machiko Ikegami
    Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229 3039, USA
    J Appl Physiol (1985) 92:519-26. 2002
    ..Genetically decreased levels of SP-B combined with superimposed O(2)-induced injury reveals the distinct contribution of SP-C to pulmonary function in vivo...
  35. pmc Recruited inflammatory cells mediate endotoxin-induced lung maturation in preterm fetal lambs
    Suhas G Kallapur
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, 3333 Burnet Avenue, Cincinnati, Ohio 45229 3039, USA
    Am J Respir Crit Care Med 172:1315-21. 2005
    ..In preterm lambs, intraamniotic endotoxin and interleukin 1 (IL-1) induce lung inflammation followed by lung maturation...
  36. ncbi request reprint C/EBPalpha is required for lung maturation at birth
    Prithy C Martis
    Division of Pulmonary Biology and Neonatology, Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Development 133:1155-64. 2006
    ..C/EBPalpha participates in a transcriptional network that is required for the regulation of genes mediating perinatal lung maturation and surfactant homeostasis that is necessary for adaptation to air breathing at birth...
  37. pmc Gene expression and biological processes influenced by deletion of Stat3 in pulmonary type II epithelial cells
    Yan Xu
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH, USA
    BMC Genomics 8:455. 2007
    ..Differentially expressed genes were identified from Affymetrix Murine GeneChips analysis and subjected to gene ontology classification, promoter analysis, pathway mapping and literature mining...
  38. ncbi request reprint Partial SP-B deficiency perturbs lung function and causes air space abnormalities
    Lori L Nesslein
    Cincinnati Children s Hospital Medical Center, Division of Neonatology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 288:L1154-61. 2005
    ..Chronic, nonuniform SP-B deficiency perturbed pulmonary function and caused air space enlargement...
  39. pmc Genomic profile of matrix and vasculature remodeling in TGF-alpha induced pulmonary fibrosis
    William D Hardie
    Division of Pulmonary Medicine, Cincinnati Children s Hospital Medical Center, University of Cincinnati School of Medicine, Cincinnati, Ohio 45229, USA
    Am J Respir Cell Mol Biol 37:309-21. 2007
    ..These studies support a role for epithelial cell-derived TGF-alpha in the regulation of processes that alter the airway and vascular architecture and function...
  40. pmc Betamethasone for lung maturation: testing dose and formulation in fetal sheep
    Alan H Jobe
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    Am J Obstet Gynecol 197:523.e1-6. 2007
    ..We evaluated lung maturation responses to the mixture of betamethasone phosphate (Beta-PO4) + betamethasone acetate (Beta-Ac) in comparison to Beta-PO4 or Beta-Ac in fetal sheep...
  41. pmc Conditional hypoxia inducible factor-1α induction in embryonic pulmonary epithelium impairs maturation and augments lymphangiogenesis
    James P Bridges
    Perinatal Institute, Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
    Dev Biol 362:24-41. 2012
    ..Collectively, these data indicate that HIF-1a protein levels in the pulmonary epithelium must be tightly controlled for proper development of the epithelial and mesenchymal compartments...
  42. ncbi request reprint Intersubunit disulfide bridge is not required for the protective role of SP-B against lung inflammation
    Machiko Ikegami
    Division of Pulmonary Biology, Cincinnati Children s Hospital, Cincinnati, Ohio 45229 3039, USA
    J Appl Physiol (1985) 93:505-11. 2002
    ..Overall, increased inflammation in SP-Bmon(+) mice was corrected to a large extent by increased gene dosage, indicating that formation of the intersubunit disulfide bridge is not critical for SP-B function...
  43. ncbi request reprint SP-B deficiency causes respiratory failure in adult mice
    Kristin R Melton
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 285:L543-9. 2003
    ..Reduction of alveolar SP-B content causes surfactant dysfunction and respiratory failure, indicating that SP-B is required for postnatal lung function...
  44. pmc Activation of sterol-response element-binding proteins (SREBP) in alveolar type II cells enhances lipogenesis causing pulmonary lipotoxicity
    Laurent Plantier
    Section of Neonatology, Perinatal and Pulmonary Biology, Perinatal Institute, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 287:10099-114. 2012
    ..Deletion of Insig1/2 activated SREBP-enhancing lipogenesis in respiratory epithelial cells resulting in lipotoxicity-related lung inflammation and tissue remodeling...
  45. pmc MEK-ERK pathway modulation ameliorates pulmonary fibrosis associated with epidermal growth factor receptor activation
    Satish K Madala
    Department of Pulmonary Medicine, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Am J Respir Cell Mol Biol 46:380-8. 2012
    ..These findings demonstrate that MEK inhibition prevents progression of established fibrosis in the TGF-α model, and provides proof of concept of targeting the MEK pathway in fibrotic lung disease...
  46. ncbi request reprint Surfactant and physiologic responses of preterm lambs to continuous positive airway pressure
    Neil Mulrooney
    Cincinnati Children s Hospital, Division of Pulmonary Biology, University of Cincinnati School of Medicine, Ohio 45229 3039, USA
    Am J Respir Crit Care Med 171:488-93. 2005
    ..A small amount of endogenous Sat PC is required for preterm lambs to breathe successfully with CPAP. CPAP 8 improves early newborn respiratory transition relative to CPAP 5...
  47. ncbi request reprint Complementation of pulmonary abnormalities in SP-D(-/-) mice with an SP-D/conglutinin fusion protein
    Liqian Zhang
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 277:22453-9. 2002
    ..Our findings also strongly suggest that the molecular mechanisms underlying impaired pulmonary host defense and abnormal lipid metabolism are distinct from those that promote ongoing inflammation and the development of emphysema...
  48. ncbi request reprint Postnatal lung inflammation increased by ventilation of preterm lambs exposed antenatally to Escherichia coli endotoxin
    Machiko Ikegami
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229 3039, USA
    Pediatr Res 52:356-62. 2002
    ..An antenatal exposure of the fetal lung to endotoxin enhanced the subsequent inflammatory response of the ventilated preterm lung...
  49. doi request reprint Toll-like receptors and agonist responses in the developing fetal sheep lung
    Noah H Hillman
    Cincinnati Children s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio 45229, USA
    Pediatr Res 63:388-93. 2008
    ..We conclude that TLR2 and TLR4 mRNAs increase through gestation and expression of TLR2 and TLR4 are induced by LPS in the fetal sheep lung...
  50. ncbi request reprint EGF receptor tyrosine kinase inhibitors diminish transforming growth factor-alpha-induced pulmonary fibrosis
    William D Hardie
    Division of Pulmonary Medicine, Cincinnati Children s Hospital Medical Center, 3333 Burnet Ave, Cincinnati, OH 45229, USA
    Am J Physiol Lung Cell Mol Physiol 294:L1217-25. 2008
    ....
  51. ncbi request reprint Increased IP-10 and MIG expression after intra-amniotic endotoxin in preterm lamb lung
    Suhas G Kallapur
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    Am J Respir Crit Care Med 167:779-86. 2003
    ..IP-10 and MIG are potent angiostatic chemokines that may contribute to lung injury and altered pulmonary vascular development in the preterm exposed to chorioamnionitis...
  52. ncbi request reprint Monocyte function in preterm, term, and adult sheep
    Boris W Kramer
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, OH 45229 3039, USA
    Pediatr Res 54:52-7. 2003
    ..The reduced phagocytosis of apoptotic cells by monocytes from the preterm may contribute to prolonged inflammation in diseases such as bronchopulmonary dysplasia...
  53. pmc Deletion of Scap in alveolar type II cells influences lung lipid homeostasis and identifies a compensatory role for pulmonary lipofibroblasts
    Valérie Besnard
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, The University of Cincinnati College of Medicine, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 284:4018-30. 2009
    ..Deletion of the SCAP/SREBP pathway in respiratory epithelial cells altered lung lipid homeostasis and induced compensatory lipid accumulation and synthesis in lung lipofibroblasts...
  54. pmc Inhibition of the αvβ6 integrin leads to limited alteration of TGF-α-induced pulmonary fibrosis
    Satish K Madala
    Dept of Pulmonary Medicine, Cincinnati Children s Hospital Medical Center, 3333 Burnet Ave, Cincinnati, OH 45229
    Am J Physiol Lung Cell Mol Physiol 306:L726-35. 2014
    ....
  55. pmc CCAAT/enhancer binding protein-α regulates the protease/antiprotease balance required for bronchiolar epithelium regeneration
    Atsuyasu Sato
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 47:454-63. 2012
    ..Treatment with serine protease inhibitor may aid in the recovery of injured bronchiolar epithelial cells, and prevent common chronic lung diseases...
  56. pmc Orphan G protein-coupled receptor GPR116 regulates pulmonary surfactant pool size
    James P Bridges
    Perinatal Institute, Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Am J Respir Cell Mol Biol 49:348-57. 2013
    ..Collectively, these data support the concept that GPR116 functions as a molecular sensor of alveolar surfactant lipid pool sizes by regulating surfactant secretion. ..
  57. ncbi request reprint Injury, inflammation, and remodeling in fetal sheep lung after intra-amniotic endotoxin
    Boris W Kramer
    Division of Pulmonary Biology, Cincinnati Children s Hospital, Cincinnati, Ohio 45229, USA
    Am J Physiol Lung Cell Mol Physiol 283:L452-9. 2002
    ..Intra-amniotic endotoxin caused surfactant secretion, inflammation, cell death, and remodeling as indications of lung injury. The recovery phase was accompanied by maturational changes in the fetal lung...
  58. ncbi request reprint Effects of high PCO2 on ventilated preterm lamb lungs
    Marya Strand
    Division of Pulmonary Biology, Cincinnati Children s Hospital, Cincinnati, Ohio 45229 3039, USA
    Pediatr Res 53:468-72. 2003
    ..Preterm lambs tolerated a very high PCO(2) without physiologic compromise for 6 h. The high PCO(2) may attenuate ventilator-induced lung injury in the preterm...
  59. ncbi request reprint Vascular changes after intra-amniotic endotoxin in preterm lamb lungs
    Suhas G Kallapur
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 287:L1178-85. 2004
    ..Exposure to antenatal inflammation may cause vascular remodeling and contribute to the development of BPD...
  60. ncbi request reprint Stat3 is required for cytoprotection of the respiratory epithelium during adenoviral infection
    Yohei Matsuzaki
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    J Immunol 177:527-37. 2006
    ..Thus, Stat3 plays a critical cytoprotective role that is required for epithelial cell survival and maintenance of alveolar structures during the early phases of pulmonary adenoviral infection...
  61. ncbi request reprint Pulmonary and systemic induction of SAA3 after ventilation and endotoxin in preterm lambs
    Teicha C Wilson
    Cincinnati Children s Hospital Medical Center, University of Cincinnati College of Medicine, Division of Pulmonary Biology, OH 45229, USA
    Pediatr Res 58:1204-9. 2005
    ..Our experiments show rapid induction of an APR gene in lung in response to proinflammatory stimuli...
  62. pmc Conditional deletion of Abca3 in alveolar type II cells alters surfactant homeostasis in newborn and adult mice
    Valérie Besnard
    The Perinatal Institute and Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics and University of Cincinnati College of Medicine, Ohio
    Am J Physiol Lung Cell Mol Physiol 298:L646-59. 2010
    ..Compensatory surfactant synthesis was initiated in ABCA3-sufficient type II cells, indicating that surfactant homeostasis is a highly regulated process that includes sensing and coregulation among alveolar type II cells...
  63. pmc Surfactant protein-d inhibits lung inflammation caused by ventilation in premature newborn lambs
    Atsuyasu Sato
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Crit Care Med 181:1098-105. 2010
    ..Although surfactant protein (SP)-D regulates pulmonary inflammation, SP-D levels are low in the preterm lung. Commercial surfactants for treatment of respiratory distress syndrome do not contain SP-D...
  64. pmc Inhibitors of inflammation and endogenous surfactant pool size as modulators of lung injury with initiation of ventilation in preterm sheep
    Noah H Hillman
    Cincinnati Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, OH 45236, USA
    Respir Res 11:151. 2010
    ..Increased pro-inflammatory cytokines in tracheal aspirates correlate with the development of BPD in preterm infants. Ventilation of preterm lambs increases pro-inflammatory cytokines and causes lung inflammation...
  65. pmc Stat-3 is required for pulmonary homeostasis during hyperoxia
    Isamu Hokuto
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Clin Invest 113:28-37. 2004
    ..Expression of Stat-3 in respiratory epithelial cells is not required for lung formation, but plays a critical role in maintenance of surfactant homeostasis and lung function during oxygen injury...
  66. ncbi request reprint Lung inflammatory responses to intratracheal interleukin-1alpha in ventilated preterm lambs
    Neil Mulrooney
    Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Pediatr Res 55:682-7. 2004
    ..The i.v. IL-1alpha caused no lung inflammation or injury but did result in severe neutropenia and hypotension leading to early death. IT IL-1alpha can cause intense lung inflammation and systemic shock in ventilated preterm lungs...
  67. pmc Inhibition of PI3K by PX-866 prevents transforming growth factor-alpha-induced pulmonary fibrosis
    Timothy D Le Cras
    Divisions of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Am J Pathol 176:679-86. 2010
    ..These data show that PI3K is activated in TGFalpha/EGFR-mediated pulmonary fibrosis and support further studies to determine the role of PI3K activation in human lung fibrotic disease, which could be amenable to targeted therapy...
  68. ncbi request reprint Intratracheal endotoxin causes systemic inflammation in ventilated preterm lambs
    Boris W Kramer
    Children s Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, Ohio 45229 3039, USA
    Am J Respir Crit Care Med 165:463-9. 2002
    ..Inflammation in the lungs was more severe in preterm animals. Mechanical ventilation of the endotoxin-exposed preterm lung resulted in systemic effects at a low endotoxin dose and without high tidal volume ventilation...
  69. ncbi request reprint Surface tension influences cell shape and phagocytosis in alveolar macrophages
    Hiroko Akei
    Cincinnati Children s Hospital, Division of Pulmonary Biology, University of Cincinnati, OH 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 291:L572-9. 2006
    ..SP-B controls surface tension by forming a surfactant phospholipid film that regulates shape and nonspecific phagocytic activity of alveolar macrophages on the alveolar surface...
  70. ncbi request reprint Differential effects of maternal betamethasone and cortisol on lung maturation and growth in fetal sheep
    Alan H Jobe
    Division of Pulmonary Biology, Cincinnati Children s Hospital, and the School of Women s and Infant s Health, University of Western Australia
    Am J Obstet Gynecol 188:22-8. 2003
    ..We asked whether maternal or fetal cortisol treatments induced fetal lung maturation and growth restriction compared with betamethasone and whether medroxyprogesterone (MPA) influenced lung maturation...
  71. ncbi request reprint Maintenance of the mouse type II cell phenotype in vitro
    Ward R Rice
    Division of Pulmonary Biology, Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 283:L256-64. 2002
    ..2 +/- 2.3 mN/m. This is the first description of the synthesis and secretion of a functional surfactant complex by mouse type II cells after 7 days in primary culture...
  72. ncbi request reprint Decreased indicators of lung injury with continuous positive expiratory pressure in preterm lambs
    Alan H Jobe
    Division of Pulmonary Biology, Cincinnati Children s Hospital, Cincinnati, Ohio 45229 3039, USA
    Pediatr Res 52:387-92. 2002
    ..05). In this model in preterm lambs CPAP results in lower indicators of acute lung injury than does mechanical ventilation during the first 2 h of life...
  73. ncbi request reprint Foxa2 regulates alveolarization and goblet cell hyperplasia
    Huajing Wan
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 131:953-64. 2004
    ..FOXA2 is required for alveolarization and regulates airway epithelial cell differentiation in the postnatal lung...
  74. ncbi request reprint Inhibition of alveolarization and altered pulmonary mechanics in mice expressing GATA-6
    Cong Liu
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 285:L1246-54. 2003
    ..Normal postnatal morphogenesis of the lung depends upon precise temporal-spatial regulation of GATA-6...
  75. pmc Leptin does not influence surfactant synthesis in fetal sheep and mice lungs
    Atsuyasu Sato
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, University of Cincinnati, Ohio, 45229 3039, USA
    Am J Physiol Lung Cell Mol Physiol 300:L498-505. 2011
    ..Furthermore, the key genes critically required for surfactant synthesis were not affected by leptin treatment...
  76. ncbi request reprint Physiological consequences of intrauterine insults
    Suhas G Kallapur
    Cincinnati Children s Hospital Medical Center, University of Cincinnati, Division of Pulmonary Biology, Ohio 45229 3039, USA
    Paediatr Respir Rev 7:110-6. 2006
    ..The information derived from clinical studies is juxtaposed with data from animal experiments to discuss the physiological consequences of intrauterine insults on fetal lung growth...
  77. ncbi request reprint Intra-amniotic endotoxin induces lung maturation by direct effects on the developing respiratory tract in preterm sheep
    Timothy J M Moss
    School of Women s and Infants Health, University of Western Australia, 35 Stirling Highway, Crawley WA, Perth, Australia
    Am J Obstet Gynecol 187:1059-65. 2002
    ..Our purpose was to determine whether improved preterm lung function caused by intraamniotic endotoxin treatment requires endotoxin entry into the respiratory tract...
  78. ncbi request reprint Early gestational intra-amniotic endotoxin: lung function, surfactant, and morphometry
    Timothy J M Moss
    School of Women s and Infants Health and Centre for Child Health Research, University of Western Australia, Perth, Australia
    Am J Respir Crit Care Med 165:805-11. 2002
    ....
  79. ncbi request reprint Intra-amniotic injection of IL-1 induces inflammation and maturation in fetal sheep lung
    Karen E Willet
    Division of Clinical Sciences, Center for Child Health Research, Perth, Australia
    Am J Physiol Lung Cell Mol Physiol 282:L411-20. 2002
    ..Lung compliance, lung volume, and alveolar Sat PC were positively correlated with residual alveolar wash leukocyte numbers 7 days after IL-1 treatment, suggesting a link between lung inflammation and maturation...
  80. ncbi request reprint The fetal maturational and inflammatory responses to different routes of endotoxin infusion in sheep
    John P Newnham
    Department of Obstetrics and Gynaecology, University of Western Australia at King Edward Memorial Hospital, Subiaco, Perth, Australia
    Am J Obstet Gynecol 186:1062-8. 2002
    ..This study aimed to explore the mechanisms by which endotoxin matures the lungs by determining whether the route of administration influenced the maturational responses of the fetus...
  81. ncbi request reprint Antenatal betamethasone changes cord blood monocyte responses to endotoxin in preterm lambs
    Boris W Kramer
    University Children s Hospital, 97080 Wurzburg, Germany
    Pediatr Res 55:764-8. 2004
    ..The time-dependent changes in maternal betamethasone modulation of the responses of fetal monocytes may influence immune function of the preterm lamb after delivery...
  82. ncbi request reprint IL-1 alpha causes lung inflammation and maturation by direct effects on preterm fetal lamb lungs
    Ilene R S Sosenko
    Department of Pediatrics, University of Miami School of Medicine, Miami, FL 33106, USA
    Pediatr Res 60:294-8. 2006
    ..IL-1alpha induced large increases in alveolar surfactant saturated phosphatidylcholine and increased lung gas volumes. Lung inflammation and maturation result from direct exposure of the fetal lung to a single cytokine - IL-1alpha...
  83. ncbi request reprint Intra-amniotic corticosteroids for preterm lung maturation in sheep
    Timothy J M Moss
    Lotteries Commission Perinatal Research Laboratories, School of Women s and Infants Health, University of Western Australia, Box M094, 35 Stirling Highway, Crawley, Perth, WA 6009, Australia
    Am J Obstet Gynecol 189:1389-95. 2003
    ..The purpose of this study was to compare efficacy on fetal lung maturation of intra-amniotic betamethasone or budesonide with the efficacy of maternal intramuscular betamethasone...
  84. ncbi request reprint Betamethasone effects on chorioamnionitis induced by intra-amniotic endotoxin in sheep
    John P Newnham
    School of Women s and Infants Health, University of Western Australia, King Edward Memorial Hospital, Bagot Road, Subiaco, Perth, WA 6008, Australia
    Am J Obstet Gynecol 189:1458-66. 2003
    ..We describe the effects of intra-amniotic endotoxin on cytokines and white cell responses in the membranes and amniotic fluid and investigate the hypothesis that betamethasone treatment suppresses these responses...
  85. ncbi request reprint Chorioamnionitis induced by subchorionic endotoxin infusion in sheep
    Timothy J M Moss
    Lotteries Commission Perinatal Research Laboratories, School of Women s and Infants Health, University of Western Australia, Box M094, 35 Stirling Highway, Crawley, WA 6009, Australia
    Am J Obstet Gynecol 189:1771-6. 2003
    ..This study was undertaken to determine whether subchorionic endotoxin infusion causes chorioamnionitis and preterm lung maturation, as occurs after intra-amniotic endotoxin...
  86. ncbi request reprint Variability in preterm lamb lung mechanics after intra-amniotic endotoxin is associated with changes in surfactant pool size and morphometry
    J Jane Pillow
    Centre for Child Health Research, The University of Western Australia, Perth, Australia
    Am J Physiol Lung Cell Mol Physiol 287:L992-8. 2004
    ..Despite the complex cascade of events initiated by antenatal endotoxin exposure, variability in lung tissue mechanics is associated primarily with changes in alveolar Sat PC pool and lung morphology...
  87. ncbi request reprint Endotoxin-induced chorioamnionitis modulates innate immunity of monocytes in preterm sheep
    Boris W Kramer
    University Children s Hospital, Josef Schneider Str 2, 97080 Wuerzburg, Germany
    Am J Respir Crit Care Med 171:73-7. 2005
    ..Modulation of fetal innate immune responses by exposure to inflammation may alter subsequent immune adaptation after birth...
  88. ncbi request reprint Experimental intrauterine Ureaplasma infection in sheep
    Timothy J M Moss
    School of Women s and Infants Health, The University of Western Australia, Perth, Australia
    Am J Obstet Gynecol 192:1179-86. 2005
    ..We hypothesized that intra-amniotic ureaplasmas would cause lung inflammation and alter fetal lung development...
  89. ncbi request reprint Ibuprofen-induced patent ductus arteriosus closure: physiologic, histologic, and biochemical effects on the premature lung
    Donald McCurnin
    Department of Pediatrics, University of Texas Health Science Center, San Antonio, Texas, USA
    Pediatrics 121:945-56. 2008
    ..The goal was to study the pulmonary, biochemical, and morphologic effects of a persistent patent ductus arteriosus in a preterm baboon model of bronchopulmonary dysplasia...

Research Grants10

  1. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 2005
    ..The intended studies will provide fundamental insights into the role of SP-D in the protection of the lung, as well as into the basic mechanisms determining surfactant homeostasis. ..
  2. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 1999
    ..The goal is to understand the role of SP-D in the cellular and/or structural mechanisms involved in independent regulation of phospholipid and surfactant protein homeostasis. ..
  3. Role of C/EBPalpha in Cytoprotection and Recovery from Lung Injury
    Machiko Ikegami; Fiscal Year: 2009
    ..Furthermore, the molecular mechanisms, in particular the signaling pathways regulating Cebpa, of recombinant human FGF-7 treatment for acute lung injury will be determined. ..
  4. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 2004
    ..The intended studies will provide fundamental insights into the role of SP-D in the protection of the lung, as well as into the basic mechanisms determining surfactant homeostasis. ..
  5. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 2001
    ..The goal is to understand the role of SP-D in the cellular and/or structural mechanisms involved in independent regulation of phospholipid and surfactant protein homeostasis. ..
  6. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 2000
    ..The goal is to understand the role of SP-D in the cellular and/or structural mechanisms involved in independent regulation of phospholipid and surfactant protein homeostasis. ..
  7. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 2003
    ..The intended studies will provide fundamental insights into the role of SP-D in the protection of the lung, as well as into the basic mechanisms determining surfactant homeostasis. ..
  8. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 2002
    ..The goal is to understand the role of SP-D in the cellular and/or structural mechanisms involved in independent regulation of phospholipid and surfactant protein homeostasis. ..
  9. ROLE OF SURFACTANT PROTEIN D IN SURFACTANT HOMEOSTASIS
    Machiko Ikegami; Fiscal Year: 2006
    ..The intended studies will provide fundamental insights into the role of SP-D in the protection of the lung, as well as into the basic mechanisms determining surfactant homeostasis. ..
  10. Role of C/EBPalpha in Cytoprotection and Recovery from Lung Injury
    Machiko Ikegami; Fiscal Year: 2010
    ..Furthermore, the molecular mechanisms, in particular the signaling pathways regulating Cebpa, of recombinant human FGF-7 treatment for acute lung injury will be determined. ..