James M Wells

Summary

Affiliation: Children's Hospital Medical Center
Country: USA

Publications

  1. ncbi request reprint Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation
    Billie A Moore-Scott
    Division of Developmental Biology, Cincinnati Children s Hospital Research Foundation, University of Cincinnati, Cincinnati, Ohio 45229 3039, USA
    Dev Dyn 236:1997-2003. 2007
  2. ncbi request reprint Genes expressed in the developing endocrine pancreas and their importance for stem cell and diabetes research
    James M Wells
    Division of Developmental Biology, Children s Hospital Research Foundation, Cincinnati, OH 45229 3039, USA
    Diabetes Metab Res Rev 19:191-201. 2003
  3. pmc Sox17 regulates organ lineage segregation of ventral foregut progenitor cells
    Jason R Spence
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Dev Cell 17:62-74. 2009
  4. pmc Klf5 regulates lineage formation in the pre-implantation mouse embryo
    Suh Chin J Lin
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 137:3953-63. 2010
  5. pmc Sox17 and Sox4 differentially regulate beta-catenin/T-cell factor activity and proliferation of colon carcinoma cells
    Debora Sinner
    Division of Developmental Biology, Cincinnati Children s Hospital Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229 3039, USA
    Mol Cell Biol 27:7802-15. 2007
  6. ncbi request reprint Sox17 influences the differentiation of respiratory epithelial cells
    Kwon Sik Park
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Dev Biol 294:192-202. 2006
  7. ncbi request reprint Molecular basis of vertebrate endoderm development
    Aaron M Zorn
    Division of Developmental Biology, Cincinnati Children s Hospital Research, Foundation and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Int Rev Cytol 259:49-111. 2007
  8. pmc Generating human intestinal tissue from pluripotent stem cells in vitro
    Kyle W McCracken
    Division of Developmental Biology, Cincinnati, Ohio, USA
    Nat Protoc 6:1920-8. 2011
  9. pmc Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
    Jason R Spence
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, Ohio 45229 3039, USA
    Nature 470:105-9. 2011
  10. pmc How to make an intestine
    James M Wells
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 141:752-60. 2014

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation
    Billie A Moore-Scott
    Division of Developmental Biology, Cincinnati Children s Hospital Research Foundation, University of Cincinnati, Cincinnati, Ohio 45229 3039, USA
    Dev Dyn 236:1997-2003. 2007
    ....
  2. ncbi request reprint Genes expressed in the developing endocrine pancreas and their importance for stem cell and diabetes research
    James M Wells
    Division of Developmental Biology, Children s Hospital Research Foundation, Cincinnati, OH 45229 3039, USA
    Diabetes Metab Res Rev 19:191-201. 2003
    ....
  3. pmc Sox17 regulates organ lineage segregation of ventral foregut progenitor cells
    Jason R Spence
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Dev Cell 17:62-74. 2009
    ..Restricting SOX17+ biliary progenitor cells to the ventral region of the gut requires the notch effector Hes1. Our results highlight the role of Sox17 and Hes1 in patterning and morphogenetic segregation of ventral foregut lineages...
  4. pmc Klf5 regulates lineage formation in the pre-implantation mouse embryo
    Suh Chin J Lin
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 137:3953-63. 2010
    ..These findings demonstrate that Klf5 is a dynamic regulator of all three lineages in the pre-implantation embryo by promoting the TE and epiblast lineages while suppressing the PE lineage...
  5. pmc Sox17 and Sox4 differentially regulate beta-catenin/T-cell factor activity and proliferation of colon carcinoma cells
    Debora Sinner
    Division of Developmental Biology, Cincinnati Children s Hospital Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229 3039, USA
    Mol Cell Biol 27:7802-15. 2007
    ..These findings indicate that Sox proteins can act as both antagonists and agonists of beta-catenin/TCF activity, and this mechanism may regulate Wnt signaling responses in many developmental and disease contexts...
  6. ncbi request reprint Sox17 influences the differentiation of respiratory epithelial cells
    Kwon Sik Park
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, OH 45229 3039, USA
    Dev Biol 294:192-202. 2006
    ..Sites of expression and the effects of Sox17 in vivo and in vitro are consistent with a role for Sox17 or other members of the Sox family of transcription factors in differentiation of the conducting airway epithelium...
  7. ncbi request reprint Molecular basis of vertebrate endoderm development
    Aaron M Zorn
    Division of Developmental Biology, Cincinnati Children s Hospital Research, Foundation and University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Int Rev Cytol 259:49-111. 2007
    ....
  8. pmc Generating human intestinal tissue from pluripotent stem cells in vitro
    Kyle W McCracken
    Division of Developmental Biology, Cincinnati, Ohio, USA
    Nat Protoc 6:1920-8. 2011
    ..To date, this is the only method for efficiently directing the differentiation of hPSCs into 3D human intestinal tissue in vitro...
  9. pmc Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
    Jason R Spence
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, Ohio 45229 3039, USA
    Nature 470:105-9. 2011
    ..PSC-derived human intestinal tissue should allow for unprecedented studies of human intestinal development and disease...
  10. pmc How to make an intestine
    James M Wells
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 141:752-60. 2014
    ..These are currently being used to study human development, genetic forms of disease, intestinal pathogens, metabolic disease and cancer. ..
  11. pmc Generating intestinal tissue from stem cells: potential for research and therapy
    Jonathan C Howell
    Division of Endocrinology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Regen Med 6:743-55. 2011
    ....
  12. pmc Molecular pathways controlling pancreas induction
    Kyle W McCracken
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
    Semin Cell Dev Biol 23:656-62. 2012
    ....
  13. ncbi request reprint Translational embryology: using embryonic principles to generate pancreatic endocrine cells from embryonic stem cells
    Jason R Spence
    Division of Developmental Biology, Cincinnati Children s Hospital Research Foundation, Cincinnati Ohio 45229 3039, USA
    Dev Dyn 236:3218-27. 2007
    ....
  14. pmc Generation of mice with a conditional null allele for Wntless
    April C Carpenter
    Visual Systems Group, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Genesis 48:554-8. 2010
    ..This Wls conditional null allele will be valuable in detecting novel Wnt functions in development and disease...
  15. pmc Converting human pluripotent stem cells into beta-cells: recent advances and future challenges
    Christopher N Mayhew
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA
    Curr Opin Organ Transplant 15:54-60. 2010
    ....
  16. ncbi request reprint Generation of β cells from human pluripotent stem cells: are we there yet?
    Jacqueline V Schiesser
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio
    Ann N Y Acad Sci 1311:124-37. 2014
    ..In doing this, we aim to identify how new approaches might be used to improve yield and functionality of in vitro-derived pancreatic β cells as an eventual cell-based therapy for type 1 diabetes. ..
  17. pmc Transdifferentiation of ciliated cells during repair of the respiratory epithelium
    Kwon Sik Park
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Am J Respir Cell Mol Biol 34:151-7. 2006
    ..Taken together, these findings demonstrate that ciliated epithelial cells spread and transdifferentiate into distinct epithelial cell types to repair the airway epithelium...
  18. pmc Wnt/beta-catenin signaling is required for development of the exocrine pancreas
    James M Wells
    Department of Developmental Biology, Cincinnati Children s Hospital Research 45267, Cincinnati, OH 45267, USA
    BMC Dev Biol 7:4. 2007
    ..A Pdx1-cre mouse line was used to delete a floxed beta-catenin allele specifically in the developing pancreas, and embryonic pancreata were studied by immunohistochemistry and microarray analysis...
  19. pmc Vertebrate endoderm development and organ formation
    Aaron M Zorn
    Division of Developmental Biology, Cincinnati Children s Research Foundation and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45229, USA
    Annu Rev Cell Dev Biol 25:221-51. 2009
    ....
  20. ncbi request reprint Comparative genomics of the Hlx homeobox gene and protein: conservation of structure and expression from fish to mammals
    Michael D Bates
    Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Gene 352:45-56. 2005
    ..These results suggest that the function of Hlx and the mechanisms regulating its expression are highly conserved in mammals, birds, amphibians and fish...
  21. pmc Endonuclease G is required for early embryogenesis and normal apoptosis in mice
    Jianhua Zhang
    Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    Proc Natl Acad Sci U S A 100:15782-7. 2003
    ..These findings indicate that EndoG is essential during early embryogenesis and plays a critical role in normal apoptosis and nuclear DNA fragmentation...
  22. ncbi request reprint Global expression analysis of gene regulatory pathways during endocrine pancreatic development
    Guoqiang Gu
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Development 131:165-79. 2004
    ..In addition to identifying new genes that regulate endocrine cell fate, this global gene expression analysis has uncovered informative biological trends that occur during endocrine differentiation...
  23. ncbi request reprint FGF signaling is necessary for establishing gut tube domains along the anterior-posterior axis in vivo
    Jessica Dessimoz
    ISREC, Chemin des Boveresses 155, CH1066, Epalinges Lausanne, Switzerland
    Mech Dev 123:42-55. 2006
    ..These data show that FGF signaling is critical for patterning the gut tube by promoting posterior and inhibiting anterior endoderm cell fate...
  24. ncbi request reprint Different thresholds of fibroblast growth factors pattern the ventral foregut into liver and lung
    Amanda E Serls
    Department of Pathology, University of Colorado Health Sciences Center, The Children s Hospital, 1056 East 19th Avenue, Denver, CO 80218, USA
    Development 132:35-47. 2005
    ..Together, the findings suggest that a concentration threshold of FGFs emanating from the cardiac mesoderm are involved in patterning the foregut endoderm...