Research Topics
| Bruce N AmesSummaryAffiliation: Children's Hospital Oakland Research Institute Country: USA Publications
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Detail Information
Publications
Delaying the mitochondrial decay of aging with acetylcarnitineBruce N Ames
Nutritional Genomics Center, Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
Ann N Y Acad Sci 1033:108-16. 2004..placebo. A meta-analysis of 4 clinical trials of LA for treatment of neuropathic deficits in diabetes showed significant efficacy vs. placebo...- A role for supplements in optimizing health: the metabolic tune-upBruce N Ames
Children s Hospital Oakland Research Institute, CA 94609, USA
Arch Biochem Biophys 423:227-34. 2004..Many common micronutrient deficiencies, such as iron or biotin, cause mitochondrial decay with oxidant leakage leading to accelerated aging and neural decay... 
Optimal micronutrients delay mitochondrial decay and age-associated diseasesBruce N Ames
Children s Hospital Oakland Research Institute, Nutrition and Metabolism Center, Oakland, CA 94609, USA
Mech Ageing Dev 131:473-9. 2010..The loss of enzyme function can be ameliorated by high doses of a B vitamin, which raises coenzyme levels, and indicates the importance of understanding the effects of age, or polymorphisms, on micronutrient requirements...
Low micronutrient intake may accelerate the degenerative diseases of aging through allocation of scarce micronutrients by triageBruce N Ames
Nutrition and Metabolism Center, Children s Hospital of Oakland Research Institute, Oakland, CA 94609, USA
Proc Natl Acad Sci U S A 103:17589-94. 2006..A multivitamin-mineral supplement is one low-cost way to ensure intake of the Recommended Dietary Allowance of micronutrients throughout life...- Lipoic acid and acetyl-carnitine reverse iron-induced oxidative stress in human fibroblastsAshutosh Lal
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, Oakland, California, USA
Redox Rep 13:2-10. 2008..We conclude that LA is highly effective in reversing oxidative stress arising from iron overload and that its antioxidant efficacy is further enhanced in combination with ALCAR... - Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathwaysHani Atamna
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609 1673, USA
FASEB J 22:703-12. 2008..MB may be useful to delay mitochondrial dysfunction with aging and the decrease in complex IV in Alzheimer disease... - N-tert-butyl hydroxylamine, a mitochondrial antioxidant, protects human retinal pigment epithelial cells from iron overload: relevance to macular degenerationLudmila A Voloboueva
Children s Hospital Oakland Research Institute, Nutrition and Metabolism Center, 5700 Martin Luther King Jr Way, Oakland, CA 94609 1673, USA
FASEB J 21:4077-86. 2007..NtBHA might be useful in AMD due to its potential to reduce oxidative stress, mitochondrial damage, and age-related iron accumulation, which may damage normal RPE function and lead to loss of vision... - Mineral and vitamin deficiencies can accelerate the mitochondrial decay of agingBruce N Ames
Nutrition, Metabolisms and Genomics Center, Children s Hospital Oakland Research Institute, Oakland, CA 94609, U States
Mol Aspects Med 26:363-78. 2005..An optimum intake of micronutrients could tune up metabolism and give a marked increase in health, particularly for the poor, elderly, and obese, at little cost... - Reducing mitochondrial decay with mitochondrial nutrients to delay and treat cognitive dysfunction, Alzheimer's disease, and Parkinson's diseaseJiankang Liu
Nutritional Genomic Center, Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
Nutr Neurosci 8:67-89. 2005..Thus, optimal doses of combinations of mt-nutrients to delay and repair mitochondrial decay could be a strategy for preventing and treating cognitive dysfunction, including AD and PD... - Iron accumulation during cellular senescence in human fibroblasts in vitroDavid W Killilea
Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA
Antioxid Redox Signal 5:507-16. 2003..These data indicate that iron accumulation occurs during normal cellular senescence in vitro. This accumulation of iron may contribute to the increased oxidative stress and cellular dysfunction seen in senescent cells... - Is there convincing biological or behavioral evidence linking vitamin D deficiency to brain dysfunction?Joyce C McCann
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA
FASEB J 22:982-1001. 2008..Despite residual uncertainty, recommendations for vitamin D supplementation of at-risk groups, including nursing infants, the elderly, and African-Americans appear warranted to ensure adequacy... - A nutrient-dense, high-fiber, fruit-based supplement bar increases HDL cholesterol, particularly large HDL, lowers homocysteine, and raises glutathione in a 2-wk trialMichele L Mietus-Snyder
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, Oakland, California, 94609, USA
FASEB J 26:3515-27. 2012..These results lay the groundwork for mechanistic/deconstruction experiments to identify critical bar components and putative synergistic combinations responsible for observed effects... - Association of chromosome damage detected as micronuclei with hematological diseases and micronutrient statusAshutosh Lal
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA
Mutagenesis 26:57-62. 2011..Understanding the link between micronutrient status and MN frequency will contribute towards determining optimal micronutrient intake to preserve long-term health... - Supplements and tuning up metabolismBruce N Ames
Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA
J Nutr 134:3164S-3168S. 2004 - Daily supplementation with iron increases lipid peroxidation in young women with low iron storesSarah M King
Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA
Exp Biol Med (Maywood) 233:701-7. 2008..The increased indicators of lipid peroxidation with duration of supplementation and as iron status improved suggest that providing daily nearly 100 mg iron may not be a totally innocuous regimen for correcting iron depletion in women... - Vitamin K, an example of triage theory: is micronutrient inadequacy linked to diseases of aging?Joyce C McCann
Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
Am J Clin Nutr 90:889-907. 2009.... - An overview of evidence for a causal relationship between dietary availability of choline during development and cognitive function in offspringJoyce C McCann
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609 1673, USA
Neurosci Biobehav Rev 30:696-712. 2006..Discussion topics include methodological issues, such as the importance of independent replication, causal criteria, and uncertainties in interpreting test results... - Clinical assay of four thiol amino acid redox couples by LC-MS/MS: utility in thalassemiaJung H Suh
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
J Chromatogr B Analyt Technol Biomed Life Sci 877:3418-27. 2009..The method was used to assess thiol redox states in plasma and erythrocytes isolated from healthy subjects and thalassemia patients... - Magnesium deficiency accelerates cellular senescence in cultured human fibroblastsDavid W Killilea
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
Proc Natl Acad Sci U S A 105:5768-73. 2008.... - An overview of evidence for a causal relation between iron deficiency during development and deficits in cognitive or behavioral functionJoyce C McCann
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, Oakland, CA, USA
Am J Clin Nutr 85:931-45. 2007..In children >2 y of age and in adolescents with ID-A, evidence suggests cognitive or behavioral deficits; however, the surprisingly small number of studies conducted in either humans or animals prevents a thorough assessment... - Delaying the mitochondrial decay of agingBruce N Ames
University of California, Berkeley, and Children s Hospital Oakland Research Institute, USA
Ann N Y Acad Sci 1019:406-11. 2004..Mitochondria provide energy for basic metabolic processes, and their decay with age impairs cellular metabolism and leads to cellular decline. Progress over the last decade in delaying the mitochondrial decay of aging is reviewed... - Delaying the mitochondrial decay of aging-a metabolic tune-upBruce N Ames
Department of Molecular and Cellular Biology, University of California, Berkeley, California 94609, USA
Alzheimer Dis Assoc Disord 17:S54-7. 2003 - Increasing longevity by tuning up metabolism. To maximize human health and lifespan, scientists must abandon outdated models of micronutrientsBruce N Ames
The Children's Hospital Oakland Research Institute, Oakland, CA, USA
EMBO Rep 6:S20-4. 2005 - High-dose vitamin therapy stimulates variant enzymes with decreased coenzyme binding affinity (increased K(m)): relevance to genetic disease and polymorphismsBruce N Ames
Department of Molecular and Cellular Biology, University of California, Berkeley, USA
Am J Clin Nutr 75:616-58. 2002.... - Heme deficiency may be a factor in the mitochondrial and neuronal decay of agingHani Atamna
Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
Proc Natl Acad Sci U S A 99:14807-12. 2002..Thus, heme deficiency or dysregulation may be an important and preventable component of the neurodegenerative process... - Mitochondrial decay, a major cause of aging, can be delayedBruce N Ames
University of California, Berkeley, CA, USA
J Alzheimers Dis 6:117-21. 2004..Our progress over the last decade in delaying the mitochondrial decay of aging is briefly reviewed... - An enthusiasm for metabolismBruce N Ames
Children's Hospital--Oakland Res Inst, Oakland, CA 94609-1673, USA
J Biol Chem 278:4369-80. 2003 - Are vitamin and mineral deficiencies a major cancer risk?Bruce N Ames
Nutrition Genomics Center, Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, California 94609 1673, USA
Nat Rev Cancer 2:694-704. 2002..Optimizing vitamin and mineral intake by encouraging dietary change, multivitamin and mineral supplements, and fortifying foods might therefore prevent cancer and other chronic diseases... - Biotin deficiency inhibits heme synthesis and impairs mitochondria in human lung fibroblastsHani Atamna
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
J Nutr 137:25-30. 2007..The findings are discussed in relation to aging and birth defects in humans... - Acrolein, a toxicant in cigarette smoke, causes oxidative damage and mitochondrial dysfunction in RPE cells: protection by (R)-alpha-lipoic acidLihong Jia
Children s Hospital Oakland Research Institute, Oakland, California, USA
Invest Ophthalmol Vis Sci 48:339-48. 2007.... - Measuring chromosome breaks in patients with thalassemiaTal Offer
Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA
Ann N Y Acad Sci 1054:439-44. 2005..Our findings indicate that higher levels of micronuclei frequencies are present in thalassemic RBCs... - Adaptive dysfunction of selenoproteins from the perspective of the triage theory: why modest selenium deficiency may increase risk of diseases of agingJoyce C McCann
Nutrition and Metabolism Center, Children s Hospital Oakland Research Institute, 5700 Martin Luthur King Jr Way, Oakland, CA 94609, USA
FASEB J 25:1793-814. 2011..Modest Se deficiency is common in many parts of the world; optimal intake could prevent future disease... - The metabolic tune-up: metabolic harmony and disease preventionBruce N Ames
University of California, Berkeley and Children s Hospital, Oakland, CA 94609, USA
J Nutr 133:1544S-8S. 2003.... - Unusual clustering of coefficients of variation in published articles from a medical biochemistry department in IndiaMark L Hudes
Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way Oakland, CA 94609, USA
FASEB J 23:689-703. 2009..Two of these articles were coauthored by 1 of the 3 investigators, and 1 was from the same university but a different department. We are unable to offer a statistical or biological explanation for the unusual clustering observed... - Chronic ethanol perturbs testicular folate metabolism and dietary folate deficiency reduces sex hormone levels in the Yucatan micropigLynn M Wallock-Montelius
Children s Hospital Oakland Research Institute, Oakland, California 94609, and Department of Internal Medicine, University of California, Davis 95616, USA
Biol Reprod 76:455-65. 2007..Furthermore, methionine synthase may influence the hormonal regulation of spermatogenesis... - Is docosahexaenoic acid, an n-3 long-chain polyunsaturated fatty acid, required for development of normal brain function? An overview of evidence from cognitive and behavioral tests in humans and animalsJoyce C McCann
Nutrition, Metabolism and Genomics Center, Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
Am J Clin Nutr 82:281-95. 2005.... - Folate deficiency inhibits the proliferation of primary human CD8+ T lymphocytes in vitroChantal Courtemanche
University of California, Berkeley, and Children's Hospital Oakland Research Institute, Oakland, CA 94609, USA
J Immunol 173:3186-92. 2004..These data suggest that folate status may affect the immune system by reducing the capacity of CD8(+) cells to proliferate in response to activation... - The role of Fe2+-induced lipid peroxidation in the initiation of the mitochondrial permeability transitionVladimir Gogvadze
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Arch Biochem Biophys 414:255-60. 2003..Lipid peroxidation products do not appear to participate in the initial phase of the permeability transition, but sensitize mitochondria toward MPT... - 5-Chlorouracil, a marker of DNA damage from hypochlorous acid during inflammation. A gas chromatography-mass spectrometry assayQing Jiang
Department of Molecular and Cell Biology, University of California, Berkeley, and Children's Hospital Oakland Research Institute, Oakland, California 94609, USA
J Biol Chem 278:32834-40. 2003..These findings may be relevant to the carcinogenesis associated with chronic inflammation... - Feeding acetyl-L-carnitine and lipoic acid to old rats significantly improves metabolic function while decreasing oxidative stressTory M Hagen
Department of Biochemistry and Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA
Proc Natl Acad Sci U S A 99:1870-5. 2002..Feeding ALCAR in combination with LA increased metabolism and lowered oxidative stress more than either compound alone... - Age-associated mitochondrial oxidative decay: improvement of carnitine acetyltransferase substrate-binding affinity and activity in brain by feeding old rats acetyl-L- carnitine and/or R-alpha -lipoic acidJiankang Liu
Division of Biochemistry and Molecular Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 99:1876-81. 2002..Thus, feeding old rats high levels of key mitochondrial metabolites can ameliorate oxidative damage, enzyme activity, substrate-binding affinity, and mitochondrial dysfunction... - Iron accumulation during cellular senescenceDavid W Killilea
Children's Hospital Oakland Research Institute, Oakland, California 94609, USA
Ann N Y Acad Sci 1019:365-7. 2004..Thus, iron accumulation is not a cause, but a consequence of normal cellular senescence in vitro. Senescence-associated iron accumulation may contribute to the increased oxidative stress and cellular dysfunction seen in senescent cells... - Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytesSusan T Mashiyama
Department of Cell and Molecular Biology, University of California, Berkley, CA 94720, USA
Anal Biochem 330:58-69. 2004..The assay requires 3 microg of DNA per folate-deficient sample, but more may be required for baseline DNA-uracil detection in healthy humans... - Iron deficiency and iron excess damage mitochondria and mitochondrial DNA in ratsPatrick B Walter
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 99:2264-9. 2002..Untreated iron deficiency, as well as excessive-iron supplementation, are deleterious and emphasize the importance of maintaining optimal iron intake... - Memory loss in old rats is associated with brain mitochondrial decay and RNA/DNA oxidation: partial reversal by feeding acetyl-L-carnitine and/or R-alpha -lipoic acidJiankang Liu
Division of Biochemistry and Molecular Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 99:2356-61. 2002..These results suggest that feeding ALCAR and LA to old rats improves performance on memory tasks by lowering oxidative damage and improving mitochondrial function... - Delaying brain mitochondrial decay and aging with mitochondrial antioxidants and metabolitesJiankang Liu
Division of Biochemistry and Molecular Biology, University of California, Berkeley, California 94720, USA
Ann N Y Acad Sci 959:133-66. 2002..This loss of activity due to coenzyme or substrate binding appears to be true for a number of other enzymes as well, including mitochondrial complex III and IV... - 5-Methyltetrahydrofolate inhibits photosensitization reactions and strand breaks in DNATal Offer
Nutrition and Metabolism Center, CHORI, Oakland, California, USA
FASEB J 21:2101-7. 2007..These observations suggest a new role for reduced folate in protection from ultraviolet damage and have bearing on the hypothesis that folate photodegradation influenced the evolution of human skin color... - The role of heme and iron-sulfur clusters in mitochondrial biogenesis, maintenance, and decay with ageHani Atamna
Department of Molecular and Cell Biology, University of California, Berkeley/CHORI, 5700 Martin Luther King Jr. Way, Oakland, California 94609, USA
Arch Biochem Biophys 397:345-53. 2002..We review the physiological role of iron-sulfur clusters and heme in the integrity of the mitochondria and the generation of oxidants... - Zinc deficiency induces oxidative DNA damage and increases p53 expression in human lung fibroblastsEmily Ho
University of California, Berkeley, CA 94720, USA
J Nutr 133:2543-8. 2003..Zinc adequacy appears to be necessary for maintaining DNA integrity and may be important in the prevention of DNA damage and cancer... - (R)-alpha-lipoic acid protects retinal pigment epithelial cells from oxidative damageLudmila A Voloboueva
Biophysics Graduate Group, University of California, Berkeley, USA
Invest Ophthalmol Vis Sci 46:4302-10. 2005..CONCLUSIONS: The present study suggests that the protective effect of LA involves multiple pathways and that LA could be effective against age-associated increase in oxidative stress and mitochondrial dysfunction in RPE cells... - Comparison of the effects of L-carnitine and acetyl-L-carnitine on carnitine levels, ambulatory activity, and oxidative stress biomarkers in the brain of old ratsJiankang Liu
Department of Molecular and Cell Biology, Uniersity of California, Berkeley, CA 94720, USA
Ann N Y Acad Sci 1033:117-31. 2004..However, ALC was effective, unlike L-carnitine, in decreasing oxidative damage, including MDA, oxo8dG/oxo8G, and nitrotyrosine, in old rat brain. These data suggest that ALC may be a better dietary supplement than L-carnitine... - Low intracellular zinc induces oxidative DNA damage, disrupts p53, NFkappa B, and AP1 DNA binding, and affects DNA repair in a rat glioma cell lineEmily Ho
University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 99:16770-5. 2002..Thus, low intracellular zinc status causes oxidative DNA damage and induces DNA-repair protein expression, but binding of p53 and important downstream signals leading to proper DNA repair are lost without zinc... - Gamma-tocopherol supplementation inhibits protein nitration and ascorbate oxidation in rats with inflammationQing Jiang
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
Free Radic Biol Med 33:1534-42. 2002.... - Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakesRobert A Jacob
U S Department of Agriculture ARS Western Human Nutrition Research Center, University of California at Davis, 95616 8683, USA
J Nutr 133:740-3. 2003..In healthy young men, the endogenous antioxidant defense system and a modest intake of dietary antioxidants are adequate to minimize levels of in vivo oxidant damage such that they cannot be differentiated by current methods... - Gamma-tocopherol, but not alpha-tocopherol, decreases proinflammatory eicosanoids and inflammation damage in ratsQing Jiang
Division of Biochemistry and Molecular Biology, University of California, Berkeley; and Children's Hospital Oakland Research Institute, Oakland, California 94609-1673, USA
FASEB J 17:816-22. 2003..These findings provide strong evidence that gammaT shows anti-inflammatory activities in vivo that may be important for human disease prevention and therapy... - Fe(2+) induces a transient Ca(2+) release from rat liver mitochondriaVladimir Gogvadze
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
Arch Biochem Biophys 398:198-202. 2002..When Fe(2+) accumulation is completed, reuptake of released Ca(2+) into mitochondria resumes. Finally, we propose that Fe(2+) either inhibits Ca(2+) entry at the uniporter or is transported by it into the matrix... - Gamma-tocopherol induces apoptosis in androgen-responsive LNCaP prostate cancer cells via caspase-dependent and independent mechanismsQing Jiang
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
Ann N Y Acad Sci 1031:399-400. 2004..Our study suggests that gamma-tocopherol may be valuable in the prevention and therapy for certain types of cancer... - Folate deficiency and ionizing radiation cause DNA breaks in primary human lymphocytes: a comparisonChantal Courtemanche
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California, USA
FASEB J 18:209-11. 2004..Our results also suggest that research on biological effects of low-dose radiation should take into account the nutritional status of the subjects, because folate deficiency could confound the effects of low-dose radiation... - Evidence-based decision making on micronutrients and chronic disease: long-term randomized controlled trials are not enoughBruce N Ames
Am J Clin Nutr 86:522-3; author reply 523-4. 2007 - Analysis of plasma tocopherols alpha, gamma, and 5-nitro-gamma in rats with inflammation by HPLC coulometric detectionStephan Christen
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
J Lipid Res 43:1978-85. 2002..The present HPLC method should be helpful in clarifying the precise physiological role of gammaT... - The case for strategic international alliances to harness nutritional genomics for public and personal healthJim Kaput
Center of Excellence in Nutritional Genomics, University of California, Davis, CA 95616, USA
Br J Nutr 94:623-32. 2005.... - A simple assay for frequency of chromosome breaks and loss (micronuclei) by flow cytometry of human reticulocytesTal Offer
Nutritional Genomics Center, Children's Hospital of Oakland Research Institute, Oakland, California 94609, USA
FASEB J 19:485-7. 2005..This method, unlike available micronuclei assays, allows rapid evaluation of a large number of cells and therefore should prove to be useful in monitoring of human populations for genetic damage... - Time to talk SENS: critiquing the immutability of human agingAubrey D N J de Grey
Department of Genetics, University of Cambridge, Cambridge, UK
Ann N Y Acad Sci 959:452-62; discussion 463-5. 2002..Given the major demographic consequences if it came about, this possibility merits urgent debate...