Jonas M Winchell

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. pmc Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis
    Jonas M Winchell
    National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 48:697-702. 2010
  2. doi request reprint Detection of Mycoplasma pneumoniae by real-time PCR
    Jonas M Winchell
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA
    Methods Mol Biol 943:149-58. 2013
  3. pmc Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation
    Jonas M Winchell
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 46:3116-8. 2008
  4. pmc Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR and high-resolution melt analysis
    Bernard J Wolff
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Antimicrob Agents Chemother 52:3542-9. 2008
  5. pmc Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis
    Stephanie B Schwartz
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 47:4117-20. 2009
  6. doi request reprint Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay
    Kathleen A Thurman
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Diagn Microbiol Infect Dis 70:1-9. 2011
  7. pmc Genotyping of Chlamydophila psittaci by real-time PCR and high-resolution melt analysis
    Stephanie L Mitchell
    Respiratory Disease Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS G 03, Atlanta, GA 30333, USA
    J Clin Microbiol 47:175-81. 2009
  8. pmc Multilocus variable-number tandem-repeat analysis of Mycoplasma pneumoniae clinical isolates from 1962 to the present: a retrospective study
    Alvaro J Benitez
    Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 50:3620-6. 2012
  9. doi request reprint Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory clinical specimens using a rapid real-time polymerase chain reaction assay
    Maureen H Diaz
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Diagn Microbiol Infect Dis 73:278-80. 2012
  10. pmc Comparison of real-time PCR and a microimmunofluorescence serological assay for detection of chlamydophila pneumoniae infection in an outbreak investigation
    Alvaro J Benitez
    Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 50:151-3. 2012

Collaborators

Detail Information

Publications31

  1. pmc Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis
    Jonas M Winchell
    National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 48:697-702. 2010
    ..This assay provides a useful diagnostic tool that can rapidly type Brucella isolates and has the potential to detect novel species. This approach may also prove helpful for clinical, epidemiological and veterinary investigations...
  2. doi request reprint Detection of Mycoplasma pneumoniae by real-time PCR
    Jonas M Winchell
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA
    Methods Mol Biol 943:149-58. 2013
    ..Recent studies have demonstrated the ability of this procedure to reliably identify this agent and facilitate the timely recognition of an outbreak...
  3. pmc Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation
    Jonas M Winchell
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 46:3116-8. 2008
    ..Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence...
  4. pmc Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR and high-resolution melt analysis
    Bernard J Wolff
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Antimicrob Agents Chemother 52:3542-9. 2008
    ..These assays may also allow for clinicians to select appropriate treatment options more rapidly and may provide a convenient method to conduct surveillance for genetic mutations conferring antibiotic resistance...
  5. pmc Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis
    Stephanie B Schwartz
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 47:4117-20. 2009
    ..pneumoniae are known, variants of each also exist. We used a real-time PCR high-resolution melt genotyping assay to identify clinical variants which may provide greater insight into the genetic distribution of M. pneumoniae strains...
  6. doi request reprint Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay
    Kathleen A Thurman
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Diagn Microbiol Infect Dis 70:1-9. 2011
    ..This procedure may allow for a practical and efficient means to test respiratory clinical specimens for atypical pneumonia agents in health care settings and facilitate an appropriate public health response to outbreaks...
  7. pmc Genotyping of Chlamydophila psittaci by real-time PCR and high-resolution melt analysis
    Stephanie L Mitchell
    Respiratory Disease Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS G 03, Atlanta, GA 30333, USA
    J Clin Microbiol 47:175-81. 2009
    ....
  8. pmc Multilocus variable-number tandem-repeat analysis of Mycoplasma pneumoniae clinical isolates from 1962 to the present: a retrospective study
    Alvaro J Benitez
    Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 50:3620-6. 2012
    ..The superior discriminatory capability of MLVA provides a powerful tool for greater resolution of M. pneumoniae strains and could be useful during outbreaks and epidemiological investigations...
  9. doi request reprint Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory clinical specimens using a rapid real-time polymerase chain reaction assay
    Maureen H Diaz
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Diagn Microbiol Infect Dis 73:278-80. 2012
    ..This procedure provides over 5 times faster results compared to existing methods while maintaining equivalent detection rates for specimens containing limited target organisms...
  10. pmc Comparison of real-time PCR and a microimmunofluorescence serological assay for detection of chlamydophila pneumoniae infection in an outbreak investigation
    Alvaro J Benitez
    Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 50:151-3. 2012
    ..Evaluation of specimens from 137 individuals suggests that real-time PCR holds greater utility as a diagnostic tool for early C. pneumoniae detection...
  11. pmc Clinical application of a multiplex real-time PCR assay for simultaneous detection of Legionella species, Legionella pneumophila, and Legionella pneumophila serogroup 1
    Alvaro J Benitez
    Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 51:348-51. 2013
    ..Evaluation of 21 clinical specimens and 115 clinical isolates demonstrated this assay to be a rapid, high-throughput diagnostic test with 100% specificity that may aid during legionellosis outbreaks and epidemiologic investigations...
  12. pmc Development of a novel genus-specific real-time PCR assay for detection and differentiation of Bartonella species and genotypes
    Maureen H Diaz
    Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 50:1645-9. 2012
    ....
  13. doi request reprint Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens
    Stephanie L Mitchell
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA
    Mol Cell Probes 23:309-11. 2009
    ..We report the evaluation of two distinct real-time PCR assays for rapid and specific detection of C. pneumoniae. We tested 401 clinical specimens, finding 5.7% positive, and confirmed a localized outbreak...
  14. doi request reprint Comparison of laboratory diagnostic procedures for detection of Mycoplasma pneumoniae in community outbreaks
    Kathleen A Thurman
    Respiratory Diseases Branch, Office of Workforce and Career Development, Atlanta, Georgia, USA
    Clin Infect Dis 48:1244-9. 2009
    ..A more definitive methodology for reliable detection of M. pneumoniae is needed to identify outbreaks and to prevent potentially fatal extrapulmonary complications...
  15. doi request reprint Community outbreak of Mycoplasma pneumoniae infection: school-based cluster of neurologic disease associated with household transmission of respiratory illness
    Nicholas D Walter
    Epidemic Intelligence Service, Office of Workforce and Career Development, Atlanta, Georgia, USA
    J Infect Dis 198:1365-74. 2008
    ..We investigated an outbreak of severe neurologic disease and pneumonia that occurred among students at 4 schools in Rhode Island...
  16. doi request reprint Comparison of nucleic acid extraction methods for the detection of Mycoplasma pneumoniae
    Kathleen A Thurman
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Diagn Microbiol Infect Dis 65:435-8. 2009
    ..pneumoniae, extracted, and tested using real-time polymerase chain reaction. Our data indicate that both automated extraction methods consistently outperform the manual procedures...
  17. doi request reprint Investigation of a Chlamydia pneumoniae outbreak in a Federal correctional facility in Texas
    Laura Conklin
    Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Clin Infect Dis 57:639-47. 2013
    ..Chlamydia pneumoniae illness is poorly characterized, particularly as a sole causative pathogen. We investigated a C. pneumoniae outbreak at a federal correctional facility...
  18. pmc Outbreak of pneumonia in the setting of fatal pneumococcal meningitis among US Army trainees: potential role of Chlamydia pneumoniae infection
    Fatimah S Dawood
    Epidemic Intelligence Service, Office of Workforce and Career Development assigned to Influenza Epidemiology and Prevention Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    BMC Infect Dis 11:157. 2011
    ..We investigated an outbreak of radiologically-confirmed pneumonia that was recognized after 2 fatal cases of serotype 7F pneumococcal meningitis were reported in a 303-person military trainee company (Alpha Company)...
  19. pmc Streptococcus pneumoniae serotype 15A in psychiatric unit, Rhode Island, USA, 2010-2011
    Katherine Fleming-Dutra
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA kfl
    Emerg Infect Dis 18:1889-93. 2012
    ..The outbreak subsided after implementation of antimicrobial drug prophylaxis and enhanced infection control measures...
  20. doi request reprint Epidemiology of respiratory infections caused by atypical bacteria in two Kenyan refugee camps
    Curi Kim
    U S Centers for Disease Control and Prevention CDC, Atlanta, GA, USA
    J Immigr Minor Health 14:140-5. 2012
    ..04). While the percentage of atypical pathogens identified was lower than expected, we found a significant relationship between atypical bacterial-viral co-infection and severity of disease in this refugee population...
  21. pmc Survey of legionella species found in thai soil
    Tatiana C Travis
    Respiratory Diseases Branch, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Mailstop G03, Atlanta, GA 30333, USA
    Int J Microbiol 2012:218791. 2012
    ..We obtained 115 isolates from 22/39 soil samples and used sequence-based methods to identify 12 known species of Legionella represented by 87 isolates...
  22. pmc Rapid detection of multidrug-resistant Mycobacterium tuberculosis by use of real-time PCR and high-resolution melt analysis
    Melissa V Ramirez
    Division of Tuberculosis Elimination, National Center for HIV AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 48:4003-9. 2010
    ..This method provides a rapid, robust, and inexpensive way to detect the dominant mutations known to confer MDR in M. tuberculosis strains and offers several advantages over current molecular and culture-based techniques...
  23. pmc Detection and characterization of Mycoplasma pneumoniae during an outbreak of respiratory illness at a university
    Jessica L Waller
    Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 52:849-53. 2014
    ..Continued molecular typing of this organism, particularly during outbreaks, may enhance the current understanding of the epidemiology of M. pneumoniae and may ultimately lead to a more effective public health response...
  24. pmc Prevalence of sequence types among clinical and environmental isolates of Legionella pneumophila serogroup 1 in the United States from 1982 to 2012
    Natalia A Kozak-Muiznieks
    Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 52:201-11. 2014
    ..The STs most commonly found in sporadic and outbreak-associated Lp1 populations may have an increased ability to cause disease and thus may require special attention when detected. ..
  25. doi request reprint Identification of legionella in the environment
    Natalia A Kozak
    Centers for Disease Control and Prevention, Atlanta, GA, USA
    Methods Mol Biol 954:3-25. 2013
    ..Specific properties of environmental sites that could be a source of Legionella contamination, collection of samples from such sites, and procedures for culture of these samples for Legionella are described in this chapter...
  26. pmc Engineered combined-positive-control template for real-time reverse transcription-PCR in multiple-pathogen-detection assays
    Maja Kodani
    Division of Bacterial Diseases, Respiratory Diseases Branch, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Clin Microbiol 50:1057-60. 2012
    ..We engineered an adaptable and expandable system of in vitro RNA transcripts to serve as a combined positive control for both DNA and RNA targets in multiple-pathogen-detection technologies based on real-time reverse transcription-PCR...
  27. pmc Application of TaqMan low-density arrays for simultaneous detection of multiple respiratory pathogens
    Maja Kodani
    Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 49:2175-82. 2011
    ..The TLDA card approach offers promise for rapid and simultaneous identification of multiple respiratory pathogens for outbreak investigations and disease surveillance...
  28. ncbi request reprint Life-threatening Pneumonia Caused by Macrolide-resistant Mycoplasma pneumoniae
    Yu Chia Hsieh
    From the Department of Pediatrics, Chang Gung Children s Hospital, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan Department of Laboratory Medicine, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan and Divisions of Viral Diseases and Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA
    Pediatr Infect Dis J 31:208-9. 2012
    ..This report highlights the need for studies to evaluate the optimal treatment in severe cases of macrolide-resistant M. pneumoniae pneumonia...
  29. pmc Isolation and characterization of a novel Francisella sp. from human cerebrospinal fluid and blood
    Kiersten J Kugeler
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd, Fort Collins, Colorado 80521, USA
    J Clin Microbiol 46:2428-31. 2008
    ..Microbiologic and molecular analyses indicate that this organism represents a novel Francisella sp. Clinicians and microbiologists should be aware of this new potential pathogen, as infection may be more common than recognized...
  30. pmc Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus
    Shannon L Emery
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Emerg Infect Dis 10:311-6. 2004
    ..The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection...