Research Topics
| Martin SteinauSummaryAffiliation: Centers for Disease Control and Prevention Country: USA Publications
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Detail Information
Publications
Performance of commercial reverse line blot assays for human papillomavirus genotypingMartin Steinau
WHO Human Papillomavirus Laboratory Network Global Reference Laboratory, Chronic Viral Diseases Branch, Division of High Consequence Pathogens, and National Center for Emerging and Zoonotic Infectious Diseases Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
J Clin Microbiol 50:1539-44. 2012..942), respectively. Although the assays had good concordance in the clinical samples, the greater accuracy and specificity in the plasmid panel suggest that LA has an advantage for internationally comparable genotyping studies...
Efficient DNA extraction for HPV genotyping in formalin-fixed, paraffin-embedded tissuesMartin Steinau
Chronic Viral Diseases Branch, Division of High Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Mol Diagn 13:377-81. 2011..035). Aggressive heat treatment demonstrated an advantage over traditional xylene purification protocols, resulting in higher DNA yields and increased sensitivity for HPV testing...
Gene expression profile of cervical tissue compared to exfoliated cells: impact on biomarker discoveryMartin Steinau
Centers for Disease Control and Prevention, Atlanta, GA, USA
BMC Genomics 6:64. 2005..However, because of keratinization and terminal differentiation it is not clear that these cells have an mRNA profile representative of cervical tissue, and that the profile can distinguish the lesions targeted for early detection...
Type-specific reproducibility of the Roche linear array HPV genotyping testMartin Steinau
Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector Borne and Enteric Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Clin Virol 42:412-4. 2008..Inter-assay comparability studies have been performed, but the robustness of type-specific results has neither been emphasized nor has the degree of intra-assay reproducibility been addressed...
Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cellsMartin Steinau
Division of Viral and Rickettsial Disease, Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 41, Atlanta, GA 30333, USA
Cancer Epidemiol Biomarkers Prev 16:295-301. 2007....
Human papillomavirus genotypes in high-grade cervical lesions in the United StatesSusan Hariri
Division of STD Prevention, National Center for HIV, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Infect Dis 206:1878-86. 2012..Monitoring HPV types in CIN2+ may be used to assess HPV vaccine impact...
Age-group differences in human papillomavirus types and cofactors for cervical intraepithelial neoplasia 3 among women referred to colposcopyJulia W Gargano
Division of High Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, MS C09, 1600 Clifton Rd NE, Atlanta, GA 30333, USA
Cancer Epidemiol Biomarkers Prev 21:111-21. 2012..Here, we assess age-group differences in HPV types and other risk factors for cervical intraepithelial neoplasia (CIN) grade 3 or worse (CIN3+) versus CIN0-2 in women from four colposcopy clinics...
Prevalence of human papillomavirus types in invasive vulvar cancers and vulvar intraepithelial neoplasia 3 in the United States before vaccine introductionJulia W Gargano
Epidemic Intelligence Service, Division of High Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Low Genit Tract Dis 16:471-9. 2012..The study aimed to determine the baseline prevalence of human papillomavirus (HPV) types in invasive vulvar cancer (IVC) and vulvar intraepithelial neoplasia 3 (VIN 3) cases using data from 7 US cancer registries...
Impact of HPV assay on observed population prevalenceElizabeth R Unger
Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic, Infectious Diseases proposed, Chronic Viral Diseases Branch Atlanta, GA, USA
Diagn Mol Pathol 20:101-4. 2011..Studies designed to examine temporal trends in type-specific prevalence should archive residual material to permit retesting if assays change...
HPV genotypes in high grade cervical lesions and invasive cervical carcinoma as detected by two commercial DNA assays, North Carolina, 2001-2006Susan Hariri
Division of STD Prevention, National Center for HIV AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
PLoS ONE 7:e34044. 2012..This study aimed to evaluate 2 commercial assays for HPV detection and typing using FFPE cervical specimens...
Molecular markers for early detection of cervical neoplasiaElizabeth R Unger
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Atlanta, GA 30333, USA
Dis Markers 20:103-16. 2004
Differences and changes in human papillomavirus type 16 variant status in human immunodeficiency virus-positive adults are not uncommonMartin Steinau
Centers for Disease Control and Prevention, National Center for Zoonotic, Vector Borne and Enteric Diseases, Chronic Viral Diseases Branch Atlanta, GA 30333, USA
J Gen Virol 91:2068-72. 2010..6%) instances (seven anal and two cervical) from eight different participants. Changes in HPV-16 variants in HIV-infected adults were seen most frequently when different anatomical sites were sampled, but were also observed over time...
Oral sampling and human papillomavirus genotyping in HIV-infected patientsMartin Steinau
Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Atlanta, GA, USA
J Oral Pathol Med 41:288-91. 2012..Screening may be useful, but methodologies and results have varied widely in previous studies. We conducted a pilot study in an HIV-positive population to evaluate HPV detection in four different oral sample types...
A real-time PCR assay for HPV52 detection and viral load quantificationJuanita M Onyekwuluje
Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Division of High Consequence Pathogens and Pathology, Chronic Viral Diseases Branch, Atlanta, GA 30333, USA
Clin Lab 58:61-6. 2012....
Differential-display PCR of peripheral blood for biomarker discovery in chronic fatigue syndromeMartin Steinau
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, MSG-41, Atlanta, GA 30333, USA
J Mol Med 82:750-5. 2004..The real-time reverse-transcription PCR assays that we developed for assay of these biomarkers will facilitate high-throughput testing of these additional samples...
RNA profiling in peripheral blood cells by fluorescent differential display PCRMartin Steinau
National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA
Methods Mol Biol 496:211-22. 2009..Besides the DD-PCR itself, methods are described for subsequent extraction, amplification, and sequencing of DNA from bands of interest to identify the corresponding genes...
DNA and RNA references for qRT-PCR assays in exfoliated cervical cellsMartin Steinau
Human Papillomavirus Program, Centers for Disease Control and Prevention, 1600 Clifton Rd, MS G41, Atlanta, GA 30333, USA
J Mol Diagn 8:113-8. 2006..These genes also showed the highest correlation with DNA contents in the same samples. If special attention to intraepithelial lesions is appropriate, RPL4 and PGK1 are recommended as the best combination of two genes...
Impact of human papillomavirus (HPV) vaccination on HPV 16/18-related prevalence in precancerous cervical lesionsSuzanne E Powell
Division of STD Prevention, National Center for HIV, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, NE MS E02, Atlanta, GA 30333, USA
Vaccine 31:109-13. 2012..This report describes vaccination status in women diagnosed with CIN2+ and examines HPV vaccine impact on HPV 16/18-related CIN2+...
Prevalence of HPV types in cervical specimens from an integrated healthcare delivery system: baseline assessment to measure HPV vaccine impactEileen F Dunne
Division of STD Prevention, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
Cancer Causes Control 24:403-7. 2013..We aimed to assess feasibility of specimen collection and baseline HPV prevalence in an integrated healthcare delivery system...
Aberrant mRNA processing of the maize Rp1-D rust resistance gene in wheat and barleyMichael A Ayliffe
CSIRO Plant Industry, Box 1600, Canberra, ACT, 2601, Australia
Mol Plant Microbe Interact 17:853-64. 2004....
