Research Topics
Species | Paul D SiegelSummaryAffiliation: Centers for Disease Control and Prevention Country: USA Publications
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Detail Information
Publications
Ovalbumin aeroallergen exposure-response in Brown Norway ratsP D Siegel
Bioorganic Chemistry, Analytical Service Branch, HELD NIOSH, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
Inhal Toxicol 12:245-61. 2000..In summary, sustained aeroallergen concentration-dependent changes in specific antibody responses and pulmonary inflammation have been demonstrated...
Physical-chemical and solvent considerations in evaluating the influence of carbon chain length on the skin sensitization activity of 1-bromoalkanesPaul D Siegel
Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
Toxicol Sci 107:78-84. 2009..It is concluded that multiple factors, in addition to lipid solubility, including vehicle, solvation, and retention on the skin surface contribute to the apparent potency of 1-bromoalkanes in the LLNA...- Dose-dependent thiol and immune responses to ovalbumin challenge in Brown Norway ratsNabil H Al-Humadi
HELD, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
Toxicol Ind Health 18:343-52. 2002..While the results of the present study do not support a functional role for thiols in the immune response, it is important to note the dose-dependent dramatic alteration seen in thiols following sensitization and challenge... 
Alteration of pulmonary immunity to Listeria monocytogenes by diesel exhaust particles (DEPs). II. Effects of DEPs on T-cell-mediated immune responses in ratsXue-Jun Yin
School of Pharmacy, West Virginia University, Morgantown, West Virginia 26506, USA
Environ Health Perspect 111:524-30. 2003..This T-cell immune response was sufficient to allow the Brown Norway rats to clear the bacteria at 7 days postinfection and overcome the down-regulation of the innate immunity by the acute DEP exposure...- Effect of diesel exhaust particulate (DEP) on immune responses: contributions of particulate versus organic soluble componentsPaul D Siegel
HELD, NIOSH, Morgantown, West Virginia 26505, USA
J Toxicol Environ Health A 67:221-31. 2004..It is concluded that both the particulate and adsorbed organics may contribute to DEP-mediated immune alterations... - Production, characterization and utility of a panel of monoclonal antibodies for the detection of toluene diisocyanate haptenated proteinsTinashe B Ruwona
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
J Immunol Methods 373:127-35. 2011..Preliminary studies using TDI vapor exposed cells suggest potential utility of these mAbs for both research and biomonitoring... - Zinc diethyldithiocarbamate allergenicity: potential haptenation mechanismsItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 2888, USA
Contact Dermatitis 59:79-89. 2008..Zinc diethyldithiocarbamate (ZDEC) and its disulfide, tetraethylthiuram disulfide (TETD), are rubber accelerators and contact allergens that cross-react in some individuals... - Mercaptobenzothiazole allergenicity-role of the thiol groupItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
Cutan Ocul Toxicol 27:103-16. 2008..It is concluded that the probable haptenation mechanism of MBT is through initial oxidation to MBTS with subsequent reduction to form mixed disulfides with proteins... - Airway responses in Brown Norway rats following inhalation sensitization and challenge with trimellitic anhydrideXing-Dong Zhang
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
Toxicol Sci 94:322-9. 2006.... - Increased cell proliferation in spleen and lymph nodes peripheral to contact allergen application siteItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, United States
Toxicology 257:113-6. 2009..Thus the contra-lateral draining superficial parotid node cannot be used as a control for application of contact allergen to a single ear in a modified LLNA... - Rapid and simple kinetics screening assay for electrophilic dermal sensitizers using nitrobenzenethiolItai Chipinda
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
Chem Res Toxicol 23:918-25. 2010..This simple, rapid, and inexpensive absorbance-based method has great potential for use as a preliminary screening tool for skin allergens... - Monoclonal antibodies against toluene diisocyanate haptenated proteins from vapor-exposed miceTinashe B Ruwona
Allergy and Clinical Immunology, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
Hybridoma (Larchmt) 29:221-9. 2010..These antibodies may be useful tools for the isolation of endogenous diisocyanate-modified proteins after natural or experimental exposures and for characterization of the toxicity of specific dNCOs... - Allergen content of patient problem and nonproblem gloves: relationship to allergen-specific patch-test findingsPaul D Siegel
Centers for Disease Control National Institute of Occupational Safety and Health, Morgantown, WV, USA
Dermatitis 21:77-83. 2010..The accuracy of the identification of allergen-source material and level of allergen in that allergen-source material is not known... - Analytical methodology and assessment of potential second-hand exposure to fentanyl in the hospital surgical settingBrandon F Law
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
J Addict Dis 29:51-8. 2010..No fentanyl was detected in the air during cardiovascular surgical operations from either surgical suite... - Discrimination of intact mycobacteria at the strain level: a combined MALDI-TOF MS and biostatistical analysisJustin M Hettick
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV, USA
Proteomics 6:6416-25. 2006..023 using only 18 m/z variables. In addition, using random forest analysis of MALDI-TOF MS data, it was possible to correctly classify bacterial strains as either M. tuberculosis or non-tuberculous with 100% accuracy... - Discrimination of haptens from prohaptens using the metabolically deficient Cpr(low/low) mouseItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
Toxicol Appl Pharmacol 252:268-72. 2011..Results of the present study suggest potential utility of the Cpr(low/low) mice in the LLNA to differentiate prohaptens from direct acting haptens... - Oxidation of 2-mercaptobenzothiazole in latex gloves and its possible haptenation pathwayItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
Chem Res Toxicol 20:1084-92. 2007..The data suggest that the critical functional group on MBT is the thiol, and haptenation is via the formation of mixed disulfides between the thiol group on MBT and a protein sulfhydryl group... - Disproportionated rosin dehydroabietic acid in neoprene surgical glovesPaul D Siegel
Centers for Disease Control CDC National Institute for Occupational Safety and Health NIOSH, Morgantown, WV, USA
Dermatitis 21:157-9. 2010..Potential contact allergens in neoprene (polychloroisoprene, polychloroprene) gloves have not been reported... - Asthmalike biphasic airway responses in Brown Norway rats sensitized by dermal exposure to dry trimellitic anhydride powderXing-Dong Zhang
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention. Morgantown, WV, USA
J Allergy Clin Immunol 113:320-6. 2004..CONCLUSION: TMA powder applied to the skin can lead to both immunologic sensitization and subsequent dose-dependent biphasic airway responses after TMA aerosol challenge... - Acute upper and lower respiratory effects in wildland firefightersDenise M Gaughan
National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA mail
J Occup Environ Med 50:1019-28. 2008..To assess acute respiratory effects experienced by wildland firefighters... - Fungal pigments inhibit the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of darkly pigmented fungiAmanda D Buskirk
National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
Anal Biochem 411:122-8. 2011..The addition of tricyclazole to the fungal growth media blocks fungal melanin synthesis and results in less melanized fungi that may be analyzed by MALDI-TOF MS... - Determination of the toluene diisocyanate binding sites on human serum albumin by tandem mass spectrometryJustin M Hettick
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
Anal Biochem 414:232-8. 2011..Diisocyanate-protein conjugation results in a variety of reaction products, including intra- and intermolecular crosslinking, diisocyanate self-polymerization, and diisocyanate hydrolysis... - Vapor conjugation of toluene diisocyanate to specific lysines of human albuminJustin M Hettick
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
Anal Biochem 421:706-11. 2012.... - Use of the human monocytic leukemia THP-1 cell line and co-incubation with microsomes to identify and differentiate hapten and prohapten sensitizersItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
Toxicology 280:135-43. 2011..In conclusion, modification of in vitro cell culture assays to include co-incubation with microsomes enhances identification of prohaptens and allows them to be clearly distinguished from direct-binding haptens... - Structural elucidation of isocyanate-peptide adducts using tandem mass spectrometryJustin M Hettick
Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
J Am Soc Mass Spectrom 20:1567-75. 2009..The direct observation of isocyanate binding to the N-terminus of peptides under these experimental conditions is in good agreement with previous studies on the relative reaction rate of isocyanate with amino acid functional groups... - Alteration of pulmonary immunity to Listeria monocytogenes by diesel exhaust particles (DEPs). I. Effects of DEPs on early pulmonary responsesXue-Jun Yin
School of Pharmacy, West Virginia University, Morgantown, West Virginia 26506-9530, USA
Environ Health Perspect 110:1105-11. 2002..DEPs may also suppress cell-mediated immunity by inhibiting AM secretion of IL-12, a key cytokine for the initiation of T helper type 1 cell development in Listeria infection... - Characterization of laboratory simulated road paving-like asphalt by high-performance liquid chromatography and gas chromatography-mass spectrometryBrandon F Law
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
J Occup Environ Hyg 3:343-50. 2006..Naphthalene, acenaphthene, fluorene, phenanthrene, fluoranthene, pyrene, and chrysene were identified and quantified from the fumes... - Haptenation: chemical reactivity and protein bindingItai Chipinda
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, MS L 2040, 1095 Willowdale Road, Morgantown, WV 26505, USA
J Allergy (Cairo) 2011:839682. 2011..Chemical aspects related to both reactivity and protein-specific binding are discussed... - Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometryJustin M Hettick
National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
Anal Chem 76:5769-76. 2004..This study demonstrates the potential for mass spectrometry-based identification/classification of mycobacteria... - Alteration of intracellular cysteine and glutathione levels in alveolar macrophages and lymphocytes by diesel exhaust particle exposureNabil H Al-Humadi
HELD, National Institute for Occupational Safety and Health, Morgantown, West Virginia, USA
Environ Health Perspect 110:349-53. 2002..DEP absorbed 23-31% of CYSH, cystine, and GSH, and only 8% of glutathione disulfide when incubated in cell free media. These results indicate that DEP exposure caused lung inflammation and affected thiol levels in both AM and LNC... - The LLNA: A Brief Review of Recent Advances and LimitationsStacey E Anderson
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Drive, Morgantown, WV 26505, USA
J Allergy (Cairo) 2011:424203. 2011..This report serves as a concise review which briefly summarizes the progress, advances and limitations of the assay over the last decade... - Kinetics and mechanistic studies of the hydrolysis of diisocyanate-derived bis-thiocarbamates of cysteine methyl esterItai Chipinda
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, West Virginia 26505-2888, USA
Chem Res Toxicol 19:341-50. 2006..Thiocarbamate dissociation rates and type of products formed may potentially influence antigenicity and subsequent hypersensitivity/toxic reactions following dNCO exposures... - The effect of diesel exhaust particles (DEP) and carbon black (CB) on thiol changes in pulmonary ovalbumin allergic sensitized Brown Norway ratsNabil H Al-Humadi
HELD, National Institute for Occupational Safety and Health, Morgantown, West Virginia, USA
Exp Lung Res 28:333-49. 2002..OVA exposure causes significant depletion of intracellular GSH in lymphocytes, which may play a key role in OVA-mediated immune responses... - Immunotoxicology of organic acid anhydrides (OAAs)Xing Dong Zhang
Analytical Services Branch, Health Effects Laboratory Division, NIOSH, Morgantown, WV 26505, USA
Int Immunopharmacol 2:239-48. 2002..Finally, animal models that have been developed to examine the mechanisms of disease will be discussed... - Alteration of pulmonary cytochrome p-450 system: effects of asphalt fume condensate exposureJ Y C Ma
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
J Toxicol Environ Health A 65:1247-60. 2002..Alteration of cytochrome P-450 metabolism of PACs may contribute to the AFC-induced genotoxic effects demonstrated as MN formation... - Effects of paving asphalt fume exposure on genotoxic and mutagenic activities in the rat lungH W Zhao
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
Mutat Res 557:137-49. 2004.... - Differentiation of the mechanism of micronuclei induced by cysteine and glutathione conjugates of methylenedi-p-phenyl diisocyanate from that of 4,4'-methylenedianilineB Z Zhong
Analytical Services Branch, M/S L4218, Health Effect Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505-2888, USA
Mutat Res 497:29-37. 2001..This mechanism is distinctly different from the mechanism of MN induction by MDA...