Paul D Siegel

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. ncbi request reprint Ovalbumin aeroallergen exposure-response in Brown Norway rats
    P D Siegel
    Bioorganic Chemistry, Analytical Service Branch, HELD NIOSH, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    Inhal Toxicol 12:245-61. 2000
  2. doi request reprint Physical-chemical and solvent considerations in evaluating the influence of carbon chain length on the skin sensitization activity of 1-bromoalkanes
    Paul D Siegel
    Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    Toxicol Sci 107:78-84. 2009
  3. ncbi request reprint Dose-dependent thiol and immune responses to ovalbumin challenge in Brown Norway rats
    Nabil H Al-Humadi
    HELD, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Toxicol Ind Health 18:343-52. 2002
  4. pmc Alteration of pulmonary immunity to Listeria monocytogenes by diesel exhaust particles (DEPs). II. Effects of DEPs on T-cell-mediated immune responses in rats
    Xue Jun Yin
    School of Pharmacy, West Virginia University, Morgantown, West Virginia 26506, USA
    Environ Health Perspect 111:524-30. 2003
  5. ncbi request reprint Effect of diesel exhaust particulate (DEP) on immune responses: contributions of particulate versus organic soluble components
    Paul D Siegel
    HELD, NIOSH, Morgantown, West Virginia 26505, USA
    J Toxicol Environ Health A 67:221-31. 2004
  6. doi request reprint Production, characterization and utility of a panel of monoclonal antibodies for the detection of toluene diisocyanate haptenated proteins
    Tinashe B Ruwona
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    J Immunol Methods 373:127-35. 2011
  7. ncbi request reprint Airway responses in Brown Norway rats following inhalation sensitization and challenge with trimellitic anhydride
    Xing Dong Zhang
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    Toxicol Sci 94:322-9. 2006
  8. doi request reprint Mercaptobenzothiazole allergenicity-role of the thiol group
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
    Cutan Ocul Toxicol 27:103-16. 2008
  9. doi request reprint Zinc diethyldithiocarbamate allergenicity: potential haptenation mechanisms
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 2888, USA
    Contact Dermatitis 59:79-89. 2008
  10. pmc Rapid and simple kinetics screening assay for electrophilic dermal sensitizers using nitrobenzenethiol
    Itai Chipinda
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
    Chem Res Toxicol 23:918-25. 2010

Collaborators

Detail Information

Publications42

  1. ncbi request reprint Ovalbumin aeroallergen exposure-response in Brown Norway rats
    P D Siegel
    Bioorganic Chemistry, Analytical Service Branch, HELD NIOSH, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    Inhal Toxicol 12:245-61. 2000
    ..In summary, sustained aeroallergen concentration-dependent changes in specific antibody responses and pulmonary inflammation have been demonstrated...
  2. doi request reprint Physical-chemical and solvent considerations in evaluating the influence of carbon chain length on the skin sensitization activity of 1-bromoalkanes
    Paul D Siegel
    Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    Toxicol Sci 107:78-84. 2009
    ..It is concluded that multiple factors, in addition to lipid solubility, including vehicle, solvation, and retention on the skin surface contribute to the apparent potency of 1-bromoalkanes in the LLNA...
  3. ncbi request reprint Dose-dependent thiol and immune responses to ovalbumin challenge in Brown Norway rats
    Nabil H Al-Humadi
    HELD, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Toxicol Ind Health 18:343-52. 2002
    ..While the results of the present study do not support a functional role for thiols in the immune response, it is important to note the dose-dependent dramatic alteration seen in thiols following sensitization and challenge...
  4. pmc Alteration of pulmonary immunity to Listeria monocytogenes by diesel exhaust particles (DEPs). II. Effects of DEPs on T-cell-mediated immune responses in rats
    Xue Jun Yin
    School of Pharmacy, West Virginia University, Morgantown, West Virginia 26506, USA
    Environ Health Perspect 111:524-30. 2003
    ..This T-cell immune response was sufficient to allow the Brown Norway rats to clear the bacteria at 7 days postinfection and overcome the down-regulation of the innate immunity by the acute DEP exposure...
  5. ncbi request reprint Effect of diesel exhaust particulate (DEP) on immune responses: contributions of particulate versus organic soluble components
    Paul D Siegel
    HELD, NIOSH, Morgantown, West Virginia 26505, USA
    J Toxicol Environ Health A 67:221-31. 2004
    ..It is concluded that both the particulate and adsorbed organics may contribute to DEP-mediated immune alterations...
  6. doi request reprint Production, characterization and utility of a panel of monoclonal antibodies for the detection of toluene diisocyanate haptenated proteins
    Tinashe B Ruwona
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    J Immunol Methods 373:127-35. 2011
    ..Preliminary studies using TDI vapor exposed cells suggest potential utility of these mAbs for both research and biomonitoring...
  7. ncbi request reprint Airway responses in Brown Norway rats following inhalation sensitization and challenge with trimellitic anhydride
    Xing Dong Zhang
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    Toxicol Sci 94:322-9. 2006
    ....
  8. doi request reprint Mercaptobenzothiazole allergenicity-role of the thiol group
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
    Cutan Ocul Toxicol 27:103-16. 2008
    ..It is concluded that the probable haptenation mechanism of MBT is through initial oxidation to MBTS with subsequent reduction to form mixed disulfides with proteins...
  9. doi request reprint Zinc diethyldithiocarbamate allergenicity: potential haptenation mechanisms
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 2888, USA
    Contact Dermatitis 59:79-89. 2008
    ..Zinc diethyldithiocarbamate (ZDEC) and its disulfide, tetraethylthiuram disulfide (TETD), are rubber accelerators and contact allergens that cross-react in some individuals...
  10. pmc Rapid and simple kinetics screening assay for electrophilic dermal sensitizers using nitrobenzenethiol
    Itai Chipinda
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
    Chem Res Toxicol 23:918-25. 2010
    ..This simple, rapid, and inexpensive absorbance-based method has great potential for use as a preliminary screening tool for skin allergens...
  11. doi request reprint Increased cell proliferation in spleen and lymph nodes peripheral to contact allergen application site
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, United States
    Toxicology 257:113-6. 2009
    ..Thus the contra-lateral draining superficial parotid node cannot be used as a control for application of contact allergen to a single ear in a modified LLNA...
  12. doi request reprint A murine monoclonal antibody with broad specificity for occupationally relevant diisocyanates
    Angela R Lemons
    a Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, West Virginia
    J Occup Environ Hyg 11:101-10. 2014
    ..The mAb may have additional utility in the standardization of specific IgE detection immunoassays as well as chromatographic-mass spectrometric methods to enrich dNCO adducted HSA in the plasma of occupationally exposed workers. ..
  13. pmc Monoclonal antibodies against toluene diisocyanate haptenated proteins from vapor-exposed mice
    Tinashe B Ruwona
    Allergy and Clinical Immunology, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
    Hybridoma (Larchmt) 29:221-9. 2010
    ..These antibodies may be useful tools for the isolation of endogenous diisocyanate-modified proteins after natural or experimental exposures and for characterization of the toxicity of specific dNCOs...
  14. doi request reprint Discrimination of haptens from prohaptens using the metabolically deficient Cpr(low/low) mouse
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    Toxicol Appl Pharmacol 252:268-72. 2011
    ..Results of the present study suggest potential utility of the Cpr(low/low) mice in the LLNA to differentiate prohaptens from direct acting haptens...
  15. doi request reprint Analytical methodology and assessment of potential second-hand exposure to fentanyl in the hospital surgical setting
    Brandon F Law
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    J Addict Dis 29:51-8. 2010
    ..No fentanyl was detected in the air during cardiovascular surgical operations from either surgical suite...
  16. ncbi request reprint Discrimination of intact mycobacteria at the strain level: a combined MALDI-TOF MS and biostatistical analysis
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV, USA
    Proteomics 6:6416-25. 2006
    ..023 using only 18 m/z variables. In addition, using random forest analysis of MALDI-TOF MS data, it was possible to correctly classify bacterial strains as either M. tuberculosis or non-tuberculous with 100% accuracy...
  17. ncbi request reprint Allergen content of patient problem and nonproblem gloves: relationship to allergen-specific patch-test findings
    Paul D Siegel
    Centers for Disease Control National Institute of Occupational Safety and Health, Morgantown, WV, USA
    Dermatitis 21:77-83. 2010
    ..The accuracy of the identification of allergen-source material and level of allergen in that allergen-source material is not known...
  18. doi request reprint Markers of upper airway inflammation associated with microbial exposure and symptoms in occupants of a water-damaged building
    Muge Akpinar-Elci
    Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA
    Am J Ind Med 56:522-30. 2013
    ..Water damage in buildings has been associated with reports of upper airway inflammation among occupants...
  19. doi request reprint Development of sandwich ELISAs for the detection of aromatic diisocyanate adducts
    Angela R Lemons
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA Electronic address
    J Immunol Methods 397:66-70. 2013
    ..The results of this study demonstrate potential application of these ELISAs in the identification and characterization of aromatic dNCO adducts as well as in biomonitoring occupational and environmental dNCO exposures...
  20. ncbi request reprint Asthmalike biphasic airway responses in Brown Norway rats sensitized by dermal exposure to dry trimellitic anhydride powder
    Xing Dong Zhang
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention Morgantown, WV, USA
    J Allergy Clin Immunol 113:320-6. 2004
    ..An animal model displaying both an early-phase airway response (EAR) and a late-phase airway response (LAR) after sensitization and subsequent inhalation challenge to a low-molecular-weight chemical has not been previously reported...
  21. ncbi request reprint Oxidation of 2-mercaptobenzothiazole in latex gloves and its possible haptenation pathway
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    Chem Res Toxicol 20:1084-92. 2007
    ..The data suggest that the critical functional group on MBT is the thiol, and haptenation is via the formation of mixed disulfides between the thiol group on MBT and a protein sulfhydryl group...
  22. ncbi request reprint Disproportionated rosin dehydroabietic acid in neoprene surgical gloves
    Paul D Siegel
    Centers for Disease Control CDC National Institute for Occupational Safety and Health NIOSH, Morgantown, WV, USA
    Dermatitis 21:157-9. 2010
    ..Potential contact allergens in neoprene (polychloroisoprene, polychloroprene) gloves have not been reported...
  23. doi request reprint Pyridoxylamine reactivity kinetics as an amine based nucleophile for screening electrophilic dermal sensitizers
    Itai Chipinda
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 2888, United States
    Toxicology 315:102-9. 2014
    ..76). The use of PDA serves as a simple, inexpensive amine based method that shows promise as a preliminary screening tool for electrophilic, amine-selective skin sensitizers. ..
  24. pmc Alteration of pulmonary immunity to Listeria monocytogenes by diesel exhaust particles (DEPs). I. Effects of DEPs on early pulmonary responses
    Xue Jun Yin
    School of Pharmacy, West Virginia University, Morgantown, West Virginia 26506 9530, USA
    Environ Health Perspect 110:1105-11. 2002
    ..DEPs may also suppress cell-mediated immunity by inhibiting AM secretion of IL-12, a key cytokine for the initiation of T helper type 1 cell development in Listeria infection...
  25. pmc Characterization of methylene diphenyl diisocyanate-haptenated human serum albumin and hemoglobin
    Morgen Mhike
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Anal Biochem 440:197-204. 2013
    ....
  26. doi request reprint Acute upper and lower respiratory effects in wildland firefighters
    Denise M Gaughan
    National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA mail
    J Occup Environ Med 50:1019-28. 2008
    ..To assess acute respiratory effects experienced by wildland firefighters...
  27. doi request reprint Determination of the toluene diisocyanate binding sites on human serum albumin by tandem mass spectrometry
    Justin M Hettick
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
    Anal Biochem 414:232-8. 2011
    ..Diisocyanate-protein conjugation results in a variety of reaction products, including intra- and intermolecular crosslinking, diisocyanate self-polymerization, and diisocyanate hydrolysis...
  28. doi request reprint Fungal pigments inhibit the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of darkly pigmented fungi
    Amanda D Buskirk
    National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
    Anal Biochem 411:122-8. 2011
    ..The addition of tricyclazole to the fungal growth media blocks fungal melanin synthesis and results in less melanized fungi that may be analyzed by MALDI-TOF MS...
  29. pmc Vapor conjugation of toluene diisocyanate to specific lysines of human albumin
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
    Anal Biochem 421:706-11. 2012
    ....
  30. ncbi request reprint Characterization of laboratory simulated road paving-like asphalt by high-performance liquid chromatography and gas chromatography-mass spectrometry
    Brandon F Law
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
    J Occup Environ Hyg 3:343-50. 2006
    ..Naphthalene, acenaphthene, fluorene, phenanthrene, fluoranthene, pyrene, and chrysene were identified and quantified from the fumes...
  31. doi request reprint Use of the human monocytic leukemia THP-1 cell line and co-incubation with microsomes to identify and differentiate hapten and prohapten sensitizers
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    Toxicology 280:135-43. 2011
    ..In conclusion, modification of in vitro cell culture assays to include co-incubation with microsomes enhances identification of prohaptens and allows them to be clearly distinguished from direct-binding haptens...
  32. doi request reprint Structural elucidation of isocyanate-peptide adducts using tandem mass spectrometry
    Justin M Hettick
    Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    J Am Soc Mass Spectrom 20:1567-75. 2009
    ..The direct observation of isocyanate binding to the N-terminus of peptides under these experimental conditions is in good agreement with previous studies on the relative reaction rate of isocyanate with amino acid functional groups...
  33. ncbi request reprint Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Justin M Hettick
    National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
    Anal Chem 76:5769-76. 2004
    ..This study demonstrates the potential for mass spectrometry-based identification/classification of mycobacteria...
  34. pmc Haptenation: chemical reactivity and protein binding
    Itai Chipinda
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, MS L 2040, 1095 Willowdale Road, Morgantown, WV 26505, USA
    J Allergy (Cairo) 2011:839682. 2011
    ..Chemical aspects related to both reactivity and protein-specific binding are discussed...
  35. pmc Alteration of intracellular cysteine and glutathione levels in alveolar macrophages and lymphocytes by diesel exhaust particle exposure
    Nabil H Al-Humadi
    HELD, National Institute for Occupational Safety and Health, Morgantown, West Virginia, USA
    Environ Health Perspect 110:349-53. 2002
    ..DEP absorbed 23-31% of CYSH, cystine, and GSH, and only 8% of glutathione disulfide when incubated in cell free media. These results indicate that DEP exposure caused lung inflammation and affected thiol levels in both AM and LNC...
  36. pmc The LLNA: A Brief Review of Recent Advances and Limitations
    Stacey E Anderson
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Drive, Morgantown, WV 26505, USA
    J Allergy (Cairo) 2011:424203. 2011
    ..This report serves as a concise review which briefly summarizes the progress, advances and limitations of the assay over the last decade...
  37. ncbi request reprint Kinetics and mechanistic studies of the hydrolysis of diisocyanate-derived bis-thiocarbamates of cysteine methyl ester
    Itai Chipinda
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, West Virginia 26505 2888, USA
    Chem Res Toxicol 19:341-50. 2006
    ..Thiocarbamate dissociation rates and type of products formed may potentially influence antigenicity and subsequent hypersensitivity/toxic reactions following dNCO exposures...
  38. ncbi request reprint The effect of diesel exhaust particles (DEP) and carbon black (CB) on thiol changes in pulmonary ovalbumin allergic sensitized Brown Norway rats
    Nabil H Al-Humadi
    HELD, National Institute for Occupational Safety and Health, Morgantown, West Virginia, USA
    Exp Lung Res 28:333-49. 2002
    ..OVA exposure causes significant depletion of intracellular GSH in lymphocytes, which may play a key role in OVA-mediated immune responses...
  39. ncbi request reprint Immunotoxicology of organic acid anhydrides (OAAs)
    Xing Dong Zhang
    Analytical Services Branch, Health Effects Laboratory Division, NIOSH, Morgantown, WV 26505, USA
    Int Immunopharmacol 2:239-48. 2002
    ..Finally, animal models that have been developed to examine the mechanisms of disease will be discussed...
  40. ncbi request reprint Effects of paving asphalt fume exposure on genotoxic and mutagenic activities in the rat lung
    H W Zhao
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Mutat Res 557:137-49. 2004
    ....
  41. ncbi request reprint Alteration of pulmonary cytochrome p-450 system: effects of asphalt fume condensate exposure
    J Y C Ma
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505 2888, USA
    J Toxicol Environ Health A 65:1247-60. 2002
    ..Alteration of cytochrome P-450 metabolism of PACs may contribute to the AFC-induced genotoxic effects demonstrated as MN formation...
  42. ncbi request reprint Differentiation of the mechanism of micronuclei induced by cysteine and glutathione conjugates of methylenedi-p-phenyl diisocyanate from that of 4,4'-methylenedianiline
    B Z Zhong
    Analytical Services Branch, M/S L4218, Health Effect Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505-2888, USA
    Mutat Res 497:29-37. 2001
    ..This mechanism is distinctly different from the mechanism of MN induction by MDA...