Wun Ju Shieh

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. pmc Pathologic studies on suspect animal and human cases of Rift Valley fever from an outbreak in Eastern Africa, 2006-2007
    Wun Ju Shieh
    Infectious Disease Pathology Branch, Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Am J Trop Med Hyg 83:38-42. 2010
  2. pmc Pathologic studies of fatal cases in outbreak of hand, foot, and mouth disease, Taiwan
    W J Shieh
    Centers for Disease Control and Prevention, 1600 Clifton Road, Mail Stop G32, Atlanta, GA 30333, USA
    Emerg Infect Dis 7:146-8. 2001
  3. ncbi request reprint Intestinal intussusception associated with adenovirus infection in Mexican children
    Jeannette Guarner
    Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop G32, Atlanta, GA 30333, USA
    Am J Clin Pathol 120:845-50. 2003
  4. ncbi request reprint Immunohistochemical detection of Yersinia pestis in formalin-fixed, paraffin-embedded tissue
    Jeannette Guarner
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Clin Pathol 117:205-9. 2002
  5. ncbi request reprint Pathogenesis and diagnosis of human meningococcal disease using immunohistochemical and PCR assays
    Jeannette Guarner
    Infectious Disease Pathology Activity, Division of Viral Rickettsial Diseases, National Centers for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Clin Pathol 122:754-64. 2004
  6. ncbi request reprint A novel coronavirus associated with severe acute respiratory syndrome
    Thomas G Ksiazek
    Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 348:1953-66. 2003
  7. ncbi request reprint Intussusception after administration of the rhesus tetravalent rotavirus vaccine (Rotashield): the search for a pathogenic mechanism
    Maureen Lynch
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Pediatrics 117:e827-32. 2006
  8. pmc Alteration of the phenotypic and pathogenic patterns of Burkholderia pseudomallei that persist in a soil environment
    Yao Shen Chen
    Division of Infectious Diseases, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan Graduate Institute of Science Education and Environmental Education, National Kaohsiung Normal University, Kaohsiung Taiwan Department of Internal Medicine, National Yang Ming University, Taipei, Taiwan Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan Department of Biotechnology, National Kaohsiung Normal University, Kaoshiung, Taiwan
    Am J Trop Med Hyg 90:469-79. 2014
  9. ncbi request reprint Morphologic, immunologic, and molecular methods to detect bacillus anthracis in formalin-fixed tissues
    Kathleen M Tatti
    Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Viral and Rickettsial Disease, Infectious Disease Pathology Activity, Atlanta, GA, USA
    Appl Immunohistochem Mol Morphol 14:234-43. 2006
  10. ncbi request reprint Fatal toxic shock syndrome associated with Clostridium sordellii after medical abortion
    Marc Fischer
    Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 353:2352-60. 2005

Detail Information

Publications44

  1. pmc Pathologic studies on suspect animal and human cases of Rift Valley fever from an outbreak in Eastern Africa, 2006-2007
    Wun Ju Shieh
    Infectious Disease Pathology Branch, Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Am J Trop Med Hyg 83:38-42. 2010
    ..Pathologic studies on postmortem tissue samples can help establish the diagnosis of RVF, differentiating from endemic diseases with clinical manifestations similar to RVF, such as malaria, leptospirosis, or yellow fever...
  2. pmc Pathologic studies of fatal cases in outbreak of hand, foot, and mouth disease, Taiwan
    W J Shieh
    Centers for Disease Control and Prevention, 1600 Clifton Road, Mail Stop G32, Atlanta, GA 30333, USA
    Emerg Infect Dis 7:146-8. 2001
    ..Pathologic studies of two fatal cases with similar clinical features revealed two different causative agents, emphasizing the need for postmortem examinations and modern pathologic techniques in an outbreak investigation...
  3. ncbi request reprint Intestinal intussusception associated with adenovirus infection in Mexican children
    Jeannette Guarner
    Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop G32, Atlanta, GA 30333, USA
    Am J Clin Pathol 120:845-50. 2003
    ..The assays used in this study proved useful for the identification of species C adenoviruses in formalin-fixed specimens from Mexican pediatric patients with intussusception...
  4. ncbi request reprint Immunohistochemical detection of Yersinia pestis in formalin-fixed, paraffin-embedded tissue
    Jeannette Guarner
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Clin Pathol 117:205-9. 2002
    ..The immunohistochemical assay provided a fast, nonhazardous method for diagnosing plague. The immunohistochemical assay localizes bacteria, retaining tissue morphologic features, and can help define transmission mechanisms...
  5. ncbi request reprint Pathogenesis and diagnosis of human meningococcal disease using immunohistochemical and PCR assays
    Jeannette Guarner
    Infectious Disease Pathology Activity, Division of Viral Rickettsial Diseases, National Centers for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Clin Pathol 122:754-64. 2004
    ..Immunohistochemical analysis determined the distribution of meningococci and meningococcal antigens in tissue samples, allowing better insights into N meningitidis pathogenesis...
  6. ncbi request reprint A novel coronavirus associated with severe acute respiratory syndrome
    Thomas G Ksiazek
    Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 348:1953-66. 2003
    ..We conducted studies to identify the etiologic agent of this outbreak...
  7. ncbi request reprint Intussusception after administration of the rhesus tetravalent rotavirus vaccine (Rotashield): the search for a pathogenic mechanism
    Maureen Lynch
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Pediatrics 117:e827-32. 2006
    ....
  8. pmc Alteration of the phenotypic and pathogenic patterns of Burkholderia pseudomallei that persist in a soil environment
    Yao Shen Chen
    Division of Infectious Diseases, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan Graduate Institute of Science Education and Environmental Education, National Kaohsiung Normal University, Kaohsiung Taiwan Department of Internal Medicine, National Yang Ming University, Taipei, Taiwan Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan Department of Biotechnology, National Kaohsiung Normal University, Kaoshiung, Taiwan
    Am J Trop Med Hyg 90:469-79. 2014
    ..We suggest that specific phenotypic and pathogenic patterns can be induced through infection with B. pseudomallei that has been cultured in different (soil versus mouse) environments...
  9. ncbi request reprint Morphologic, immunologic, and molecular methods to detect bacillus anthracis in formalin-fixed tissues
    Kathleen M Tatti
    Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Viral and Rickettsial Disease, Infectious Disease Pathology Activity, Atlanta, GA, USA
    Appl Immunohistochem Mol Morphol 14:234-43. 2006
    ..anthracis sequences derived from formalin-fixed tissues of anthrax cases. In this study, morphologic, immunologic, and molecular assays were used to study and diagnose 22 veterinary and human anthrax cases...
  10. ncbi request reprint Fatal toxic shock syndrome associated with Clostridium sordellii after medical abortion
    Marc Fischer
    Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 353:2352-60. 2005
    ..These cases indicate the need for physician awareness of this syndrome and for further study of its association with medical abortion...
  11. ncbi request reprint Molecular diagnosis of Nocardia farcinica from a cerebral abscess
    Kathleen M Tatti
    Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Atlanta, Georgia 30333, USA
    Hum Pathol 37:1117-21. 2006
    ..A species-specific polymerase chain reaction assay confirmed N farcinica as the etiologic agent...
  12. ncbi request reprint Detection of West Nile virus in formalin-fixed, paraffin-embedded human tissues by RT-PCR: a useful adjunct to conventional tissue-based diagnostic methods
    Julu Bhatnagar
    Infectious Disease Pathology Activity, Centers for Disease Control and Prevention, 1600 Clifton Road, N E, Mailstop G32, Atlanta, GA 30333, United States
    J Clin Virol 38:106-11. 2007
    ....
  13. ncbi request reprint CD8-mediated protection against Ebola virus infection is perforin dependent
    Manisha Gupta
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Immunol 174:4198-202. 2005
    ..These findings suggest that the clearance of Ebola virus is perforin-dependent and provide an additional example showing that this basic immunologic mechanism is not limited to the clearance of noncytopathic viruses...
  14. ncbi request reprint Influenza-associated deaths among children in the United States, 2003-2004
    Niranjan Bhat
    Epidemic Intelligence Service, Career Development Division, Office of Workforce and Career Development National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    N Engl J Med 353:2559-67. 2005
    ..Although influenza is common among children, pediatric mortality related to laboratory-confirmed influenza has not been assessed nationally...
  15. ncbi request reprint Human monkeypox infection: a family cluster in the midwestern United States
    James J Sejvar
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA
    J Infect Dis 190:1833-40. 2004
    ..We describe a cluster of monkeypox in a family with a spectrum of clinical illness, including encephalitis, and outline the laboratory confirmation of monkeypox...
  16. pmc Monkeypox transmission and pathogenesis in prairie dogs
    Jeannette Guarner
    Infectious Disease Pathology Activity, Centers for Disease Control and Prevention, Mailstop G32, 1600 Clifton Road NE, Atlanta, GA 30333, USA
    Emerg Infect Dis 10:426-31. 2004
    ..Prairie dogs offer insights into transmission, pathogenesis, and new vaccine and treatment trials because they are susceptible to severe monkeypox infection...
  17. pmc Variant Creutzfeldt-Jakob disease death, United States
    Ermias D Belay
    Division of Viral and Rickettsial Disease, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 11:1351-4. 2005
    ..The reports of bloodborne transmission of vCJD in 2 patients, 1 of whom was heterozygous for methionine and valine at polymorphic codon 129, add to the uncertainty about the future of the vCJD outbreak...
  18. pmc Persistent infection with ebola virus under conditions of partial immunity
    Manisha Gupta
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 78:958-67. 2004
    ....
  19. pmc Diagnosis of influenza from respiratory autopsy tissues: detection of virus by real-time reverse transcription-PCR in 222 cases
    Amy M Denison
    Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    J Mol Diagn 13:123-8. 2011
    ..We also determined that testing of all available respiratory tissues is critical for optimal detection of influenza virus in postmortem tissues...
  20. pmc 2009 pandemic influenza A (H1N1): pathology and pathogenesis of 100 fatal cases in the United States
    Wun Ju Shieh
    Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 32, Atlanta, GA 30333, USA
    Am J Pathol 177:166-75. 2010
    ..Underlying medical conditions and bacterial co-infections contributed to the fatal outcome of this infection. More studies are needed to understand the multifactorial pathogenesis of this infection...
  21. ncbi request reprint Persistent Yersinia pestis antigens in ischemic tissues of a patient with septicemic plague
    Jeannette Guarner
    Infectious Diseases Pathology Activity, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Hum Pathol 36:850-3. 2005
    ....
  22. pmc Surveillance for unexplained deaths and critical illnesses due to possibly infectious causes, United States, 1995-1998
    Rana A Hajjeh
    Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 8:145-53. 2002
    ..The UNEX network model would improve U.S. diagnostic capacities and preparedness for emerging infections...
  23. ncbi request reprint Molecular and immunological methods to detect rotavirus in formalin-fixed tissue
    Kathleen M Tatti
    Division of Viral and Rickettsial Disease, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Infectious Disease Pathology Activity, 1600 Clifton Road, NE, Mailstop G30, Atlanta, GA 30333, USA
    J Virol Methods 105:305-19. 2002
    ..These techniques should prove valuable to detect rotavirus antigens and nucleic acids in tissues from patients infected naturally with rotavirus or with intussususception associated with rotavirus vaccine...
  24. doi request reprint Pathology and pathogenesis of fatal Bordetella pertussis infection in infants
    Christopher D Paddock
    Infectious Disease Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Clin Infect Dis 47:328-38. 2008
    ....
  25. ncbi request reprint Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches
    Cynthia S Goldsmith
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Mailstop G30, 1600 Clifton Road NE, Atlanta, GA 30333, USA
    Virus Res 92:89-98. 2003
    ..Ultrastructural ISH studies suggest these inclusions play an important role in the transcription process...
  26. ncbi request reprint Toxic shock associated with Clostridium sordellii and Clostridium perfringens after medical and spontaneous abortion
    Adam L Cohen
    Prevention and Response Branch, Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Obstet Gynecol 110:1027-33. 2007
    ..To better understand the risk of fatal toxic shock caused by Clostridium sordellii in women who had a recent medical abortion with mifepristone and misoprostol...
  27. pmc Investigation of bioterrorism-related anthrax, United States, 2001: epidemiologic findings
    Daniel B Jernigan
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Emerg Infect Dis 8:1019-28. 2002
    ..Continued vigilance for cases is needed among health-care providers and members of the public health and law enforcement communities...
  28. ncbi request reprint Histopathologic and immunohistochemical features of fatal influenza virus infection in children during the 2003-2004 season
    Jeannette Guarner
    Infectious Disease Pathology Activity, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Clin Infect Dis 43:132-40. 2006
    ..The Centers for Disease Control and Prevention enhanced national surveillance for influenza-associated deaths among children because of early reports of pediatric deaths during the 2003-2004 influenza season...
  29. doi request reprint Histopathologic and immunohistochemical characterization of Nipah virus infection in the guinea pig
    F J Torres-Velez
    College of Veterinary Medicine, Department of Veterinary Pathology, 501 DW Brooks Drive, Athens, GA 30602 7388 USA
    Vet Pathol 45:576-85. 2008
    ....
  30. ncbi request reprint A novel immunohistochemical assay for the detection of Ebola virus in skin: implications for diagnosis, spread, and surveillance of Ebola hemorrhagic fever. Commission de Lutte contre les Epidémies à Kikwit
    S R Zaki
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Infect Dis 179:S36-47. 1999
    ..IHC testing of formalin-fixed skin specimens is a safe, sensitive, and specific method for laboratory diagnosis of EHF and should be useful for EHF surveillance and prevention...
  31. ncbi request reprint Long-term disease surveillance in Bandundu region, Democratic Republic of the Congo: a model for early detection and prevention of Ebola hemorrhagic fever
    E S Lloyd
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Infect Dis 179:S274-80. 1999
    ....
  32. ncbi request reprint Transmission of Black Creek Canal virus between cotton rats
    K L Hutchinson
    Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Med Virol 60:70-6. 2000
    ..J. Med. Virol. 60:70-76, 2000. Published 2000 Wiley-Liss, Inc...
  33. pmc Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States
    J A Jernigan
    NCID, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 7:933-44. 2001
    ..With multidrug antibiotic regimens and supportive care, survival of patients (60%) was markedly higher (<15%) than previously reported...
  34. ncbi request reprint Usefulness of immunohistochemical diagnosis of streptococcus pneumoniae in formalin-fixed, paraffin-embedded specimens compared with culture and gram stain techniques
    Jeannette Guarner
    Infectious Diseases Pathology Activity, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Clin Pathol 127:612-8. 2007
    ..Compared with culture, the immunohistochemical assay showed 100% sensitivity and 71% specificity. Immunohistochemical analysis has the diagnostic advantage of correlating host inflammatory reaction with presence of pneumococci...
  35. ncbi request reprint Histopathologic, immunohistochemical, and polymerase chain reaction assays in the study of cases with fatal sporadic myocarditis
    Jeannette Guarner
    Infectious Disease Pathology Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Hum Pathol 38:1412-9. 2007
    ..Accurately identifying an infectious agent is the foundation for clinical and public health interventions. Despite using multiple diagnostic methods, an organism could only be detected in a small proportion of sporadic myocarditis cases...
  36. ncbi request reprint Diagnosis of invasive group a streptococcal infections by using immunohistochemical and molecular assays
    Jeannette Guarner
    Infectious Disease Pathology Activity, National Centers for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Clin Pathol 126:148-55. 2006
    ..Eighteen patients had systemic infection. Immunohistochemical analysis and PCR can be used for diagnosis of GAS infections in formalin-fixed, paraffin-embedded samples...
  37. ncbi request reprint The pathology of rotavirus-associated deaths, using new molecular diagnostics
    Maureen Lynch
    Viral Gastroenteritis Section, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Clin Infect Dis 37:1327-33. 2003
    ..The part played by rotavirus in fatal cases is unclear and requires additional investigation of diarrhea-associated deaths, because a better understanding might alter the approach to treatment and the need for antiviral therapy...
  38. ncbi request reprint Fatal West Nile virus encephalitis in a renal transplant recipient
    Melissa M Cushing
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA
    Am J Clin Pathol 121:26-31. 2004
    ..Immunosuppression of the transplant recipient might have been responsible for the fulminant viral effects. The pathologic diagnosis helped guide subsequent epidemiologic and laboratory studies...
  39. ncbi request reprint Immunohistochemical, in situ hybridization, and ultrastructural localization of SARS-associated coronavirus in lung of a fatal case of severe acute respiratory syndrome in Taiwan
    Wun Ju Shieh
    Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, Center for Disease Control and Prevention, Atlanta, GA 30333, USA
    Hum Pathol 36:303-9. 2005
    ..These techniques represent valuable laboratory diagnostic modalities and provide insights into the pathogenesis of this emerging infection...
  40. doi request reprint Lower respiratory tract hemorrhage associated with 2009 pandemic influenza A (H1N1) virus infection
    Erin D Kennedy
    Epidemiology and Prevention Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Influenza Other Respir Viruses 7:761-5. 2013
    ..We describe patients with LRTH associated with 2009 pandemic influenza A (H1N1) (pH1N1) virus infection identified from April 2009 to April 2010 in the United States...
  41. pmc Role of host cytokine responses in the pathogenesis of avian H5N1 influenza viruses in mice
    Kristy J Szretter
    Influenza Branch MS G 16, Division of Viral and Ricksettial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA
    J Virol 81:2736-44. 2007
    ..These results suggest that TNF-alpha may contribute to morbidity during H5N1 influenza virus infection, while IL-1 may be important for effective virus clearance in nonlethal H5N1 disease...
  42. pmc The role of pathology in an investigation of an outbreak of West Nile encephalitis in New York, 1999
    W J Shieh
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Emerg Infect Dis 6:370-2. 2000
    ..Histopathologic and immunopathologic examinations performed on human autopsy materials helped guide subsequent laboratory and epidemiologic investigations that led to identification of the etiologic agent...
  43. ncbi request reprint Fatal malaria infection in travelers: novel immunohistochemical assays for the detection of Plasmodium falciparum in tissues and implications for pathogenesis
    Gillian L Genrich
    Infectious Disease Pathology and Malaria Branches, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Am J Trop Med Hyg 76:251-9. 2007
    ..vivax. Immunostaining patterns were predominately cytoplasmic and membranous. P. falciparum antigens were detected in a variety of organs but were most abundant in the blood vessels of brain, heart, and lung tissues...
  44. ncbi request reprint Leptospirosis mimicking acute cholecystitis among athletes participating in a triathlon
    J Guarner
    Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, and Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Hum Pathol 32:750-2. 2001
    ..If a cholecystectomy is performed in febrile patients with suspicious environmental or animal exposure, pathologic studies for leptospirosis on formalin-fixed, paraffin-embedded tissues may be of great value...