Brian H Raphael

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. doi request reprint Detection and differentiation of Clostridium botulinum type A strains using a focused DNA microarray
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Mol Cell Probes 24:146-53. 2010
  2. pmc Analysis of genomic differences among Clostridium botulinum type A1 strains
    Ping Ke Fang
    Botulinum Research Center and Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, Massachusetts 02747, USA
    BMC Genomics 11:725. 2010
  3. pmc Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    BMC Microbiol 12:245. 2012
  4. pmc Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
    Appl Environ Microbiol 74:4390-7. 2008
  5. pmc Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 78:8712-8. 2012
  6. pmc Neurotoxin gene clusters in Clostridium botulinum type Ab strains
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Appl Environ Microbiol 75:6094-101. 2009
  7. pmc Sequence diversity of genes encoding botulinum neurotoxin type F
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Appl Environ Microbiol 76:4805-12. 2010
  8. pmc Clostridium botulinum strains producing BoNT/F4 or BoNT/F5
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 80:3250-7. 2014
  9. doi request reprint Recovery and detection of botulinum neurotoxins from a nonporous surface
    Matthew Lautenschlager
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    J Microbiol Methods 92:278-80. 2013
  10. pmc First report worldwide of an infant botulism case due to Clostridium botulinum type E
    Carolina Luquez
    Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 29, Atlanta, GA 30329, USA
    J Clin Microbiol 48:326-8. 2010

Collaborators

Detail Information

Publications13

  1. doi request reprint Detection and differentiation of Clostridium botulinum type A strains using a focused DNA microarray
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Mol Cell Probes 24:146-53. 2010
    ..The focused microarray format provides a rapid approach for neurotoxin gene detection and preliminary determination of the relatedness of strains isolated from different sources...
  2. pmc Analysis of genomic differences among Clostridium botulinum type A1 strains
    Ping Ke Fang
    Botulinum Research Center and Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, Massachusetts 02747, USA
    BMC Genomics 11:725. 2010
    ..The purpose of this study was to characterize differences among these genomes and compare these differentiating features with two additional unsequenced strains used in previous studies...
  3. pmc Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    BMC Microbiol 12:245. 2012
    ..Previous studies showed that the amino acid sequences of BoNT/E produced by various strains differ by < 6% and that the type E neurotoxin gene cluster inserts into the rarA operon...
  4. pmc Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
    Appl Environ Microbiol 74:4390-7. 2008
    ..These results demonstrate surprising genomic homogeneity among a cluster of unique C. botulinum type A strains of diverse origin...
  5. pmc Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 78:8712-8. 2012
    ..Four groups of strains shared a similarity of at least 95% by PFGE; the largest group included 21 out of 46 strains. The strains analyzed in this study showed relatively limited genetic diversity using either MLST or PFGE...
  6. pmc Neurotoxin gene clusters in Clostridium botulinum type Ab strains
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Appl Environ Microbiol 75:6094-101. 2009
    ..The type Ab strains examined in this study had differences in their toxin gene cluster compositions and bont/A and bont/B nucleotide sequences, suggesting that they may have arisen from separate recombination events...
  7. pmc Sequence diversity of genes encoding botulinum neurotoxin type F
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Appl Environ Microbiol 76:4805-12. 2010
    ..These results demonstrate that extensive nucleotide diversity exists among genes encoding type F neurotoxins from strains with different phylogenetic backgrounds and from various geographical sources...
  8. pmc Clostridium botulinum strains producing BoNT/F4 or BoNT/F5
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 80:3250-7. 2014
    ..Finally, these data support a role for the mobility of a bont/F5-carrying plasmid among strains of diverse genomic backgrounds. ..
  9. doi request reprint Recovery and detection of botulinum neurotoxins from a nonporous surface
    Matthew Lautenschlager
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    J Microbiol Methods 92:278-80. 2013
    ..In addition, we demonstrate that this method can be used to evaluate the efficacy of surface decontamination procedures...
  10. pmc First report worldwide of an infant botulism case due to Clostridium botulinum type E
    Carolina Luquez
    Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 29, Atlanta, GA 30329, USA
    J Clin Microbiol 48:326-8. 2010
    ..Infant botulism type E cases have been associated with neurotoxigenic strains of C. butyricum. We report the first infant botulism case due to C. botulinum type E worldwide...
  11. pmc Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F5
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA
    FEBS Lett 586:109-15. 2012
    ..Here, we report that BoNT/F5 cleaves substrate synaptobrevin-2 in a different location than the other BoNT/F subtypes, between (54)L and (55)E. This is the first report of cleavage of synaptobrevin-2 in this location...
  12. doi request reprint Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Microbiol Methods 90:267-72. 2012
    ....
  13. ncbi request reprint Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complex
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, United States
    J Microbiol Methods 71:343-6. 2007
    ..These results indicate that the NTNH real-time PCR assay can be used to screen enrichment cultures of primary specimens at earlier time points (24 h) than by toxin detection of unknown culture supernatants (up to 5 days)...