Research Topics
Species | Brian H RaphaelSummaryAffiliation: Centers for Disease Control and Prevention Country: USA Publications
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Detail Information
Publications
Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtypeBrian H Raphael
Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
BMC Microbiol 12:245. 2012..Previous studies showed that the amino acid sequences of BoNT/E produced by various strains differ by < 6% and that the type E neurotoxin gene cluster inserts into the rarA operon...
Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking waterBrian H Raphael
Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Microbiol Methods 90:267-72. 2012....
Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complexBrian H Raphael
Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, United States
J Microbiol Methods 71:343-6. 2007..These results indicate that the NTNH real-time PCR assay can be used to screen enrichment cultures of primary specimens at earlier time points (24 h) than by toxin detection of unknown culture supernatants (up to 5 days)...- Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clustersBrian H Raphael
Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
Appl Environ Microbiol 74:4390-7. 2008..These results demonstrate surprising genomic homogeneity among a cluster of unique C. botulinum type A strains of diverse origin... - Sequence diversity of genes encoding botulinum neurotoxin type FBrian H Raphael
Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
Appl Environ Microbiol 76:4805-12. 2010..These results demonstrate that extensive nucleotide diversity exists among genes encoding type F neurotoxins from strains with different phylogenetic backgrounds and from various geographical sources... - Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genesCarolina Luquez
Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Appl Environ Microbiol 78:8712-8. 2012..Four groups of strains shared a similarity of at least 95% by PFGE; the largest group included 21 out of 46 strains. The strains analyzed in this study showed relatively limited genetic diversity using either MLST or PFGE... - Detection and differentiation of Clostridium botulinum type A strains using a focused DNA microarrayBrian H Raphael
Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
Mol Cell Probes 24:146-53. 2010..The focused microarray format provides a rapid approach for neurotoxin gene detection and preliminary determination of the relatedness of strains isolated from different sources... - Neurotoxin gene clusters in Clostridium botulinum type Ab strainsCarolina Luquez
Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Appl Environ Microbiol 75:6094-101. 2009..The type Ab strains examined in this study had differences in their toxin gene cluster compositions and bont/A and bont/B nucleotide sequences, suggesting that they may have arisen from separate recombination events... - Recovery and detection of botulinum neurotoxins from a nonporous surfaceMatthew Lautenschlager
Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
J Microbiol Methods 92:278-80. 2013..In addition, we demonstrate that this method can be used to evaluate the efficacy of surface decontamination procedures... - Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F5Suzanne R Kalb
Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA
FEBS Lett 586:109-15. 2012..Here, we report that BoNT/F5 cleaves substrate synaptobrevin-2 in a different location than the other BoNT/F subtypes, between (54)L and (55)E. This is the first report of cleavage of synaptobrevin-2 in this location... - First report worldwide of an infant botulism case due to Clostridium botulinum type ECarolina Luquez
Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 29, Atlanta, GA 30329, USA
J Clin Microbiol 48:326-8. 2010..Infant botulism type E cases have been associated with neurotoxigenic strains of C. butyricum. We report the first infant botulism case due to C. botulinum type E worldwide... - Characterization of genetically matched isolates of Campylobacter jejuni reveals that mutations in genes involved in flagellar biosynthesis alter the organism's virulence potentialPreeti Malik Kale
School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4233, USA
Appl Environ Microbiol 73:3123-36. 2007..Collectively, these findings support the hypothesis that critical differences in gene content or gene expression can alter the pathogenic potential of C. jejuni isolates... - FeoB is not required for ferrous iron uptake in Campylobacter jejuniBrian H Raphael
Department of Veterinary Science and Microbiology, The University of Arizona, Tucson 85721, USA
Can J Microbiol 49:727-31. 2003..jejuni feoB genes are defective. In addition, uptake could not be attributed to the magnesium transporter CorA, since no reduction in 55Fe2+ uptake was observed in the presence of a CorA-specific inhibitor...