Brian H Raphael

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. ncbi request reprint Distinguishing highly-related outbreak-associated Clostridium botulinum type A(B) strains
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    BMC Microbiol 14:192. 2014
  2. pmc Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    BMC Microbiol 12:245. 2012
  3. doi request reprint Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Microbiol Methods 90:267-72. 2012
  4. pmc Sequence diversity of genes encoding botulinum neurotoxin type F
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Appl Environ Microbiol 76:4805-12. 2010
  5. ncbi request reprint Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complex
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, United States
    J Microbiol Methods 71:343-6. 2007
  6. pmc Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
    Appl Environ Microbiol 74:4390-7. 2008
  7. doi request reprint Detection and differentiation of Clostridium botulinum type A strains using a focused DNA microarray
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Mol Cell Probes 24:146-53. 2010
  8. pmc Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 78:8712-8. 2012
  9. pmc Neurotoxin gene clusters in Clostridium botulinum type Ab strains
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Appl Environ Microbiol 75:6094-101. 2009
  10. pmc Clostridium botulinum strains producing BoNT/F4 or BoNT/F5
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 80:3250-7. 2014

Detail Information

Publications15

  1. ncbi request reprint Distinguishing highly-related outbreak-associated Clostridium botulinum type A(B) strains
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    BMC Microbiol 14:192. 2014
    ..botulinum PulseNet database is composed of A(B) strains. The purpose of this study was to evaluate the ability of genome sequencing and multi-loci variable number of tandem repeat analysis (MLVA) to differentiate such strains...
  2. pmc Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    BMC Microbiol 12:245. 2012
    ..Previous studies showed that the amino acid sequences of BoNT/E produced by various strains differ by < 6% and that the type E neurotoxin gene cluster inserts into the rarA operon...
  3. doi request reprint Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Microbiol Methods 90:267-72. 2012
    ....
  4. pmc Sequence diversity of genes encoding botulinum neurotoxin type F
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Appl Environ Microbiol 76:4805-12. 2010
    ..These results demonstrate that extensive nucleotide diversity exists among genes encoding type F neurotoxins from strains with different phylogenetic backgrounds and from various geographical sources...
  5. ncbi request reprint Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complex
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, United States
    J Microbiol Methods 71:343-6. 2007
    ..These results indicate that the NTNH real-time PCR assay can be used to screen enrichment cultures of primary specimens at earlier time points (24 h) than by toxin detection of unknown culture supernatants (up to 5 days)...
  6. pmc Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
    Appl Environ Microbiol 74:4390-7. 2008
    ..These results demonstrate surprising genomic homogeneity among a cluster of unique C. botulinum type A strains of diverse origin...
  7. doi request reprint Detection and differentiation of Clostridium botulinum type A strains using a focused DNA microarray
    Brian H Raphael
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    Mol Cell Probes 24:146-53. 2010
    ..The focused microarray format provides a rapid approach for neurotoxin gene detection and preliminary determination of the relatedness of strains isolated from different sources...
  8. pmc Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 78:8712-8. 2012
    ..Four groups of strains shared a similarity of at least 95% by PFGE; the largest group included 21 out of 46 strains. The strains analyzed in this study showed relatively limited genetic diversity using either MLST or PFGE...
  9. pmc Neurotoxin gene clusters in Clostridium botulinum type Ab strains
    Carolina Luquez
    Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Appl Environ Microbiol 75:6094-101. 2009
    ..The type Ab strains examined in this study had differences in their toxin gene cluster compositions and bont/A and bont/B nucleotide sequences, suggesting that they may have arisen from separate recombination events...
  10. pmc Clostridium botulinum strains producing BoNT/F4 or BoNT/F5
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Appl Environ Microbiol 80:3250-7. 2014
    ..Finally, these data support a role for the mobility of a bont/F5-carrying plasmid among strains of diverse genomic backgrounds. ..
  11. doi request reprint Recovery and detection of botulinum neurotoxins from a nonporous surface
    Matthew Lautenschlager
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    J Microbiol Methods 92:278-80. 2013
    ..In addition, we demonstrate that this method can be used to evaluate the efficacy of surface decontamination procedures...
  12. pmc Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F5
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA
    FEBS Lett 586:109-15. 2012
    ..Here, we report that BoNT/F5 cleaves substrate synaptobrevin-2 in a different location than the other BoNT/F subtypes, between (54)L and (55)E. This is the first report of cleavage of synaptobrevin-2 in this location...
  13. pmc First report worldwide of an infant botulism case due to Clostridium botulinum type E
    Carolina Luquez
    Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 29, Atlanta, GA 30329, USA
    J Clin Microbiol 48:326-8. 2010
    ..Infant botulism type E cases have been associated with neurotoxigenic strains of C. butyricum. We report the first infant botulism case due to C. botulinum type E worldwide...
  14. pmc Characterization of genetically matched isolates of Campylobacter jejuni reveals that mutations in genes involved in flagellar biosynthesis alter the organism's virulence potential
    Preeti Malik-Kale
    School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4233, USA
    Appl Environ Microbiol 73:3123-36. 2007
    ..Collectively, these findings support the hypothesis that critical differences in gene content or gene expression can alter the pathogenic potential of C. jejuni isolates...
  15. ncbi request reprint FeoB is not required for ferrous iron uptake in Campylobacter jejuni
    Brian H Raphael
    Department of Veterinary Science and Microbiology, The University of Arizona, Tucson 85721, USA
    Can J Microbiol 49:727-31. 2003
    ..jejuni feoB genes are defective. In addition, uptake could not be attributed to the magnesium transporter CorA, since no reduction in 55Fe2+ uptake was observed in the presence of a CorA-specific inhibitor...