Mangalathu S Rajeevan

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. pmc Functional genomics of serotonin receptor 2A (HTR2A): interaction of polymorphism, methylation, expression and disease association
    Virginia R Falkenberg
    Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Neuromolecular Med 13:66-76. 2011
  2. pmc Evaluation of DNA extraction from granulocytes discarded in the separation medium after isolation of peripheral blood mononuclear cells and plasma from whole blood
    Janna R Murray
    Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, 1600 Clifton Rd Mailstop G41, Atlanta, GA 30333, USA
    BMC Res Notes 6:440. 2013
  3. ncbi request reprint Quantitation of site-specific HPV 16 DNA methylation by pyrosequencing
    Mangalathu S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MSG41, Atlanta, GA 30333, USA
    J Virol Methods 138:170-6. 2006
  4. ncbi request reprint Glucocorticoid receptor polymorphisms and haplotypes associated with chronic fatigue syndrome
    M S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Genes Brain Behav 6:167-76. 2007
  5. pmc Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR
    M S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U S Department of Health and Human Services, Atlanta, GA 30333, USA
    J Mol Diagn 3:26-31. 2001
  6. ncbi request reprint Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies
    M S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Ceners for Disease Control and Prevention, US Department of Health and Public Services, Atlanta, Georgia 30333, USA
    Methods 25:443-51. 2001
  7. ncbi request reprint Global amplification of sense RNA: a novel method to replicate and archive mRNA for gene expression analysis
    Mangalathu S Rajeevan
    Viral Exanthems and Herpesvirus Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, US Department of Health and Human Services, Atlanta, GA 30333, USA
    Genomics 82:491-7. 2003
  8. pmc Integrated weighted gene co-expression network analysis with an application to chronic fatigue syndrome
    Angela P Presson
    Biostatistics, University of California, Los Angeles, CA, USA
    BMC Syst Biol 2:95. 2008
  9. pmc Determination of quantitative and site-specific DNA methylation of perforin by pyrosequencing
    Supraja Narasimhan
    Division of Viral and Rickettsial Diseases, National Center for Zoonotic Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    BMC Res Notes 2:104. 2009
  10. ncbi request reprint Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells
    Martin Steinau
    Division of Viral and Rickettsial Disease, Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 41, Atlanta, GA 30333, USA
    Cancer Epidemiol Biomarkers Prev 16:295-301. 2007

Collaborators

Detail Information

Publications27

  1. pmc Functional genomics of serotonin receptor 2A (HTR2A): interaction of polymorphism, methylation, expression and disease association
    Virginia R Falkenberg
    Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Neuromolecular Med 13:66-76. 2011
    ..Confirmation in an independent study population is required...
  2. pmc Evaluation of DNA extraction from granulocytes discarded in the separation medium after isolation of peripheral blood mononuclear cells and plasma from whole blood
    Janna R Murray
    Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, 1600 Clifton Rd Mailstop G41, Atlanta, GA 30333, USA
    BMC Res Notes 6:440. 2013
    ..This report provides a method for extracting DNA using the PAXgene Blood DNA kit from granulocytes without purifying them from the separation medium...
  3. ncbi request reprint Quantitation of site-specific HPV 16 DNA methylation by pyrosequencing
    Mangalathu S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MSG41, Atlanta, GA 30333, USA
    J Virol Methods 138:170-6. 2006
    ..Pyrosequencing provides a quantitative site-specific assessment of methylation at multiple CpG sites without cloning, and is thus suited to large-scale molecular epidemiologic studies...
  4. ncbi request reprint Glucocorticoid receptor polymorphisms and haplotypes associated with chronic fatigue syndrome
    M S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Genes Brain Behav 6:167-76. 2007
    ....
  5. pmc Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR
    M S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U S Department of Health and Human Services, Atlanta, GA 30333, USA
    J Mol Diagn 3:26-31. 2001
    ..Real-time RT-PCR based on LightCycler technology is well-suited to validate DNA array results because it is quantitative, rapid, and requires 1000-fold less RNA than conventional assays...
  6. ncbi request reprint Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies
    M S Rajeevan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Ceners for Disease Control and Prevention, US Department of Health and Public Services, Atlanta, Georgia 30333, USA
    Methods 25:443-51. 2001
    ....
  7. ncbi request reprint Global amplification of sense RNA: a novel method to replicate and archive mRNA for gene expression analysis
    Mangalathu S Rajeevan
    Viral Exanthems and Herpesvirus Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, US Department of Health and Human Services, Atlanta, GA 30333, USA
    Genomics 82:491-7. 2003
    ..00001-0.1%). This reflects the high fidelity of sRNA synthesis for mRNA as low as 0.3 copies/cell. sRNA is amplified synthetic mRNA in the 5'-->3' direction; the appropriate template for any gene expression analysis...
  8. pmc Integrated weighted gene co-expression network analysis with an application to chronic fatigue syndrome
    Angela P Presson
    Biostatistics, University of California, Los Angeles, CA, USA
    BMC Syst Biol 2:95. 2008
    ..Here we show that the additional inclusion of genetic marker data allows one to characterize network relationships as causal or reactive in a chronic fatigue syndrome (CFS) data set...
  9. pmc Determination of quantitative and site-specific DNA methylation of perforin by pyrosequencing
    Supraja Narasimhan
    Division of Viral and Rickettsial Diseases, National Center for Zoonotic Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    BMC Res Notes 2:104. 2009
    ..PBMC DNA may be better suited than whole blood DNA for examining methylation levels in genes associated with immune function...
  10. ncbi request reprint Evaluation of RNA markers for early detection of cervical neoplasia in exfoliated cervical cells
    Martin Steinau
    Division of Viral and Rickettsial Disease, Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 41, Atlanta, GA 30333, USA
    Cancer Epidemiol Biomarkers Prev 16:295-301. 2007
    ....
  11. pmc Gene expression profile of cervical tissue compared to exfoliated cells: impact on biomarker discovery
    Martin Steinau
    Centers for Disease Control and Prevention, Atlanta, GA, USA
    BMC Genomics 6:64. 2005
    ..However, because of keratinization and terminal differentiation it is not clear that these cells have an mRNA profile representative of cervical tissue, and that the profile can distinguish the lesions targeted for early detection...
  12. ncbi request reprint Genetic evaluation of the serotonergic system in chronic fatigue syndrome
    Alicia K Smith
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MSG41, Atlanta, GA 30333, USA
    Psychoneuroendocrinology 33:188-97. 2008
    ..These data indicate that sequence variation in HTR2A, potentially resulting in its enhanced activity, may be involved in the pathophysiology of CFS...
  13. doi request reprint An angiotensin-1 converting enzyme polymorphism is associated with allostatic load mediated by C-reactive protein, interleukin-6 and cortisol
    Alicia K Smith
    Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, MSG41, Atlanta, GA 30333, USA
    Psychoneuroendocrinology 34:597-606. 2009
    ....
  14. ncbi request reprint Epidemiologic and viral factors associated with cervical neoplasia in HPV-16-positive women
    Mangalathu S Rajeevan
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Int J Cancer 115:114-20. 2005
    ..Cellular proliferation and differentiation pathways affected by HPV should be investigated as biomarkers for cervical cancer screening...
  15. ncbi request reprint Gene expression profiling of dysplastic differentiation in cervical epithelial cells harboring human papillomavirus 16
    Daya G Ranamukhaarachchi
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service US DHHS, Atlanta, GA 30333, USA
    Genomics 85:727-38. 2005
    ..Some of the genes with altered expression identified in this model of dysplastic differentiation may be useful biomarkers for early detection of cervical neoplasia and other HPV-associated oropharyngeal and anogenital cancers...
  16. ncbi request reprint Human papillomavirus type 16 variant assignment by pyrosequencing
    David C Swan
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Atlanta, GA 30333, USA
    J Virol Methods 136:166-70. 2006
    ..Pyrosequencing of 97 HPV16-positive exfoliated cervical samples confirmed the Sanger sequencing results; however pyrosequencing identified additional variants in several samples containing mixed variants...
  17. ncbi request reprint Polymorphisms in genes regulating the HPA axis associated with empirically delineated classes of unexplained chronic fatigue
    Alicia K Smith
    Centers for Disease Control and Prevention, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, 1600 Clifton Road, MSG41, Atlanta, GA 30333, USA
    Pharmacogenomics 7:387-94. 2006
    ..These data support the hypothesis that medically unexplained chronic fatigue is heterogeneous and presents preliminary evidence of the genetic mechanisms underlying some of the putative conditions...
  18. ncbi request reprint Differential-display PCR of peripheral blood for biomarker discovery in chronic fatigue syndrome
    Martin Steinau
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, MSG 41, Atlanta, GA 30333, USA
    J Mol Med (Berl) 82:750-5. 2004
    ..The real-time reverse-transcription PCR assays that we developed for assay of these biomarkers will facilitate high-throughput testing of these additional samples...
  19. doi request reprint RNA profiling in peripheral blood cells by fluorescent differential display PCR
    Martin Steinau
    National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA
    Methods Mol Biol 496:211-22. 2009
    ..Besides the DD-PCR itself, methods are described for subsequent extraction, amplification, and sequencing of DNA from bands of interest to identify the corresponding genes...
  20. pmc Convergent genomic studies identify association of GRIK2 and NPAS2 with chronic fatigue syndrome
    Alicia K Smith
    Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Neuropsychobiology 64:183-94. 2011
    ....
  21. pmc Transcriptional control of complement activation in an exercise model of chronic fatigue syndrome
    Bristol Sorensen
    Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, United States of America
    Mol Med 15:34-42. 2009
    ..MASP2 down-regulation may act as an antiinflammatory acute-phase response in healthy subjects, whereas its elevated level may account for increased C4a and inflammation-mediated postexertional malaise in CFS subjects...
  22. pmc Molecular markers for early detection of cervical neoplasia
    Elizabeth R Unger
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Atlanta, GA 30333, USA
    Dis Markers 20:103-16. 2004
  23. pmc Identification of Phosphoglycerate Kinase 1 (PGK1) as a reference gene for quantitative gene expression measurements in human blood RNA
    Virginia R Falkenberg
    Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA, 30333, USA
    BMC Res Notes 4:324. 2011
    ..abstract:..
  24. ncbi request reprint Modifying differential display polymerase chain reaction to detect relative changes in gene expression profiles
    Daya G Ranamukhaarachchi
    Viral Exanthems and Herpesvirus Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U S Department of Health and Human Services, Atlanta, GA 30333, USA
    Anal Biochem 306:343-6. 2002
  25. pmc DNA and RNA references for qRT-PCR assays in exfoliated cervical cells
    Martin Steinau
    Human Papillomavirus Program, Centers for Disease Control and Prevention, 1600 Clifton Rd, MS G41, Atlanta, GA 30333, USA
    J Mol Diagn 8:113-8. 2006
    ..These genes also showed the highest correlation with DNA contents in the same samples. If special attention to intraepithelial lesions is appropriate, RPL4 and PGK1 are recommended as the best combination of two genes...
  26. doi request reprint Identification of a potential molecular link between the glucocorticoid and serotonergic signaling systems
    Virginia R Falkenberg
    Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Mol Neurosci 41:322-7. 2010
    ..This finding provides new approaches to studies directed at mechanisms for glucocorticoid negative feedback regulation of the HPA axis involving 5-HT and interventional studies directed to neuropsychiatric diseases...
  27. doi request reprint Human papillomavirus prevalence in invasive anal cancers in the United States before vaccine introduction
    Martin Steinau
    1Chronic Viral Diseases Branch, Centers for Disease Control and Prevention, Atlanta GA 2University of Hawaii Cancer Center, University of Hawaii, Honolulu, HI 3Community and Population Health Research Institute, Department of Biomedical Sciences, Cedars Sinai Medical Center, Los Angeles, CA 4Michigan Cancer Surveillance Program, Michigan Department of Community Health, Lansing, MI 5Department of Epidemiology, University of Kentucky, Lexington, KY 6Los Angeles Cancer Registry, Los Angeles, CA 7Florida Department of Health, Tallahassee, FL 8Louisiana State University Health Sciences Center, School of Public Health, New Orleans, LA 9Department of Epidemiology, University of Iowa, Iowa City, IA 10College of Medicine, University of Florida, Gainesville, FL 11Battelle Memorial Institute, Durham, NC and 12Epidemiology and Applied Research Branch, Centers for Disease Control and Prevention, Atlanta GA
    J Low Genit Tract Dis 17:397-403. 2013
    ..This study aimed to conduct a representative survey of human papillomavirus (HPV) prevalence and its genotype distribution in invasive anal cancer specimens in the United States...