Research Topics
Genomes and GenesSpecies | R S LanciottiSummaryAffiliation: Centers for Disease Control and Prevention Country: USA Publications
| Collaborators
|
Detail Information
Publications
Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007Robert S Lanciotti
Diagnostic and Reference Laboratory, Arbovirus Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA
Emerg Infect Dis 14:1232-9. 2008..In June 2007, an epidemic of fever and rash associated with ZIKV was detected in Yap State, Federated States of Micronesia. We report the genetic and serologic properties of the ZIKV associated with this epidemic...
Chikungunya virus in US travelers returning from India, 2006Robert S Lanciotti
Diagnostic and Reference Laboratory, Arbovirus Disease Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA
Emerg Infect Dis 13:764-7. 2007..In 2005-2006, CHIKV epidemics were reported in islands in the Indian Ocean and in southern India. We present data on laboratory-confirmed CHIKV infections among travelers returning from India to the United States during 2006...
Complete genome sequences and phylogenetic analysis of West Nile virus strains isolated from the United States, Europe, and the Middle EastRobert S Lanciotti
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
Virology 298:96-105. 2002..Lineage 2 WN viruses have been isolated only in sub-Saharan Africa and Madagascar. Lineage 1 viruses can be further subdivided into three monophyletic clades...
Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis virusesR S Lanciotti
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
J Clin Microbiol 39:4506-13. 2001..These assays should be of utility in the diagnostic laboratory to complement existing diagnostic testing methodologies and as a tool in conducting flavivirus surveillance in the United States...
Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United StatesR S Lanciotti
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA
Science 286:2333-7. 1999..This North American WN virus was most closely related to a WN virus isolated from a dead goose in Israel in 1998...
Emergence of epidemic O'nyong-nyong fever in Uganda after a 35-year absence: genetic characterization of the virusR S Lanciotti
Division of Vector Borne Infectious Diseases, U S Department of Health and Human Services, Fort Collins, Colorado, USA
Virology 252:258-68. 1998..The sequence data also reveal that unlike the published ONN (1959) sequence, all ONN strains from the 1996-1997 epidemic possess a stop codon at the nsp3-nsp4 junction...
West Nile virus in overwintering Culex mosquitoes, New York City, 2000R S Nasci
CDC DVBID, PO Box 2087, Fort Collins, CO 80522, USA
Emerg Infect Dis 7:742-4. 2001..WN viral RNA and live virus were found in pools of Culex mosquitoes. Persistence in overwintering Cx. pipiens may be important in the maintenance of WN virus in the northeastern United States...
Zika virus outbreak on Yap Island, Federated States of MicronesiaMark R Duffy
Division of Vector Borne Infectious Diseases, National Center for Zoonotic, Vector Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA
N Engl J Med 360:2536-43. 2009..No previous outbreaks and only 14 cases of Zika virus disease have been previously documented...
Comparative West Nile virus detection in organs of naturally infected American Crows (Corvus brachyrhynchos)N A Panella
Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA
Emerg Infect Dis 7:754-5. 2001..The brain was the most sensitive test organ; it was the only positive organ for three of the positive crows. The sensitivity of crow organs as targets for WN virus detection makes crow death useful for WN virus surveillance...
Transmission of West Nile virus from an organ donor to four transplant recipientsMartha Iwamoto
Epidemic Intelligence Service, Division of Healthcare Quality Promotion, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta 30333, USA
N Engl J Med 348:2196-203. 2003..Organ recipients receiving immunosuppressive drugs may be at high risk for severe disease after West Nile virus infection. Blood transfusion was the probable source of the West Nile virus viremia in the organ donor...
West Nile virus isolates from mosquitoes in New York and New Jersey, 1999R S Nasci
Centers for Disease Control and Prevention DVBID, PO Box 2087, Fort Collins, CO 80522, USA
Emerg Infect Dis 7:626-30. 2001..Molecular techniques were used to identify the species represented in the WN virus-positive mosquito pools. Most isolates were from pools containing Culex pipiens mosquitoes, but several pools contained two or more Culex species...
West Nile virus infection among pregnant women in a northern Colorado community, 2003 to 2004Jan E Paisley
Department of Pediatrics and Neonatology, University of Colorado Health Sciences Center, Denver, Colorado, USA
Pediatrics 117:814-20. 2006..To determine the frequencies of WNV infections during pregnancy and of intrauterine WNV infections, we measured WNV-specific antibodies in cord blood from infant deliveries after a community-wide epidemic of WNV disease...
Molecular amplification assays for the detection of flavivirusesRobert S Lanciotti
Diagnostic and Reference Laboratory, Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA
Adv Virus Res 61:67-99. 2003..The various assay formats will be described and their utility discussed...
Comparison of vero cell plaque assay, TaqMan reverse transcriptase polymerase chain reaction RNA assay, and VecTest antigen assay for detection of West Nile virus in field-collected mosquitoesRoger S Nasci
Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, PO Box 2087, Fort Collins, CO 80522, USA
J Am Mosq Control Assoc 18:294-300. 2002..Positive and negative attributes of each procedure, which may influence how and where they are used in surveillance programs, are discussed...
The emergence of West Nile virus in North America: ecology, epidemiology, and surveillanceJ T Roehrig
Arbovirus Diseases Branch, Division of Vector-Borne Infectious Diseases, National Centers for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado, USA
Curr Top Microbiol Immunol 267:223-40. 2002..The valuable lessons learned from the detection and response to the introduction of WN virus into NYC should prove useful if and when subsequent intrusions of new disease agents occur...
La Crosse virus in Aedes albopictus mosquitoes, Texas, USA, 2009Amy J Lambert
Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA
Emerg Infect Dis 16:856-8. 2010..The presence of this virus in an invasive vector species within a region that lies outside the virus's historically recognized geographic range is of public health concern...
West Nile virus blood transfusion-related infection despite nucleic acid testingAlexandre Macedo de Oliveira
Epidemic Intelligence Service, Division of Applied Public Health Training, Epidemiology Program Office, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Transfusion 44:1695-9. 2004..WNV NAT assays might vary in sensitivity and pooling donations could further impact test performance. Understanding MP NAT limitations can improve strategies to maintain safety of the blood supply in the United States...
Validation of a microsphere-based immunoassay for detection of anti-West Nile virus and anti-St. Louis encephalitis virus immunoglobulin m antibodiesAlison J Johnson
Division of Vector Borne Infectious Diseases, National Center for Zoonotic, Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd, Foothills Campus, Fort Collins, CO 80521, USA
Clin Vaccine Immunol 14:1084-93. 2007..The detection of IgM antibodies to WN virus was more consistent than detection of IgM antibodies to SLE virus. Some changes were made to the analysis software that resulted in an improved accuracy of diagnosis...
Birth outcomes following West Nile Virus infection of pregnant women in the United States: 2003-2004Daniel R O'Leary
Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522, USA
Pediatrics 117:e537-45. 2006..Congenital West Nile virus (WNV) infection was first described in a single case in 2002. The proportion of maternal WNV infections resulting in congenital infection and clinical consequences of such infections are unknown...
Consensus amplification and novel multiplex sequencing method for S segment species identification of 47 viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus genera of the family BunyaviridaeAmy J Lambert
National Center for Zoonotic, Vector Borne, and Enteric Diseases, CDC, Fort Collins, CO 80521, USA
J Clin Microbiol 47:2398-404. 2009....
Fatal hemorrhagic fever caused by West Nile virus in the United StatesChristopher D Paddock
Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Clin Infect Dis 42:1527-35. 2006..We describe a case of fulminant WNV infection in a 59-year-old Florida man who died following a brief illness that resembled hemorrhagic disease caused by Rickettsia reckettsii, dengue virus or yellow fever virus...
Second human case of Cache Valley virus diseaseGrant L Campbell
Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA
Emerg Infect Dis 12:854-6. 2006..Cache Valley virus disease is rarely diagnosed in North America, in part because laboratories rarely test for it. Its true incidence, effect on public health, and full clinical spectrum remain to be determined...
Detection of Colorado Tick Fever viral RNA in acute human serum samples by a quantitative real-time RT-PCR assayAmy J Lambert
National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Rampert Road, Fort Collins, CO 80521, USA
J Virol Methods 140:43-8. 2007..The quantitative real-time RT-PCR assay is efficient, sensitive and specific and as such is useful for the detection of CTF viral RNA in the diagnostic or research laboratory...
Detection of West Nile virus in formalin-fixed, paraffin-embedded human tissues by RT-PCR: a useful adjunct to conventional tissue-based diagnostic methodsJulu Bhatnagar
Infectious Disease Pathology Activity, Centers for Disease Control and Prevention, 1600 Clifton Road, N E, Mailstop G32, Atlanta, GA 30333, United States
J Clin Virol 38:106-11. 2007....
Molecular characterization of medically important viruses of the genus OrthobunyavirusAmy J Lambert
Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO 80521, USA
J Gen Virol 89:2580-5. 2008....
Transfusion-associated transmission of West Nile virus, United States 2003 through 2005Susan P Montgomery
Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA
Transfusion 46:2038-46. 2006..National blood donation screening for West Nile virus (WNV) started in June 2003, after the documentation of WNV transfusion-associated transmission (TAT) in 2002...
Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assayBarbara W Johnson
Diagnostic and Reference Laboratory, Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, 3150 Rampart Rd, Fort, Collins, CO 80521
J Clin Microbiol 43:4977-83. 2005....
Virology, pathology, and clinical manifestations of West Nile virus diseaseEdward B Hayes
Centers for Disease Control and Prevention, Fort Collins, Colorado 80526, USA
Emerg Infect Dis 11:1174-9. 2005....
West Nile virus infection transmitted by blood transfusionTheresa Harrington
Epidemic Intelligence Service Branch, Division of Applied Public Health Training, Epidemiology Program Office, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Transfusion 43:1018-22. 2003..An investigation was initiated to determine a possible association between transfusion and WNV infection...
Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assaysAmy J Lambert
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Fort Collins, Colorado 80521, USA
J Clin Microbiol 41:379-85. 2003..The sensitivity, specificity, and rapidity of nucleic acid amplification demonstrate the usefulness of NASBA, standard RT-PCR, and TaqMan assays, in both research and diagnostic settings, to detect North American EEE and WEE viral RNAs...
Persistence of virus-reactive serum immunoglobulin m antibody in confirmed west nile virus encephalitis casesJohn T Roehrig
Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
Emerg Infect Dis 9:376-9. 2003..Of those patients bled, 7 (60%) of 12 had anti-WNV IgM at approximately 500 days after onset. Clinicians should be cautious when interpreting serologic results from early season WNV IgM-positive patients...
Transmission of West Nile virus through blood transfusion in the United States in 2002Lisa N Pealer
Epidemic Intelligence Service, Division of Applied Public Health Training, Epidemiology Program Office, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
N Engl J Med 349:1236-45. 2003..During the 2002 West Nile virus epidemic in the United States, patients were identified whose West Nile virus illness was temporally associated with the receipt of transfused blood and blood components...
Evaluation of a diagnostic algorithm using immunoglobulin M enzyme-linked immunosorbent assay to differentiate human West Nile Virus and St. Louis Encephalitis virus infections during the 2002 West Nile Virus epidemic in the United StatesDenise A Martin
Centers for Disease Control and Prevention, Rampart Rd, Foothills Campus, Fort Collins, Colorado 80522, USA
Clin Diagn Lab Immunol 11:1130-3. 2004..Use of the W/S ratio as part of the testing algorithm to interpret MAC-ELISA results generates reportable probable cases quickly, alleviating the need for PRNT in most instances...
Use of immunoglobulin m cross-reactions in differential diagnosis of human flaviviral encephalitis infections in the United StatesDenise A Martin
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
Clin Diagn Lab Immunol 9:544-9. 2002..Infections with SLE virus resulted in antibody that was more cross-reactive, so identification of SLE virus as the infecting agent by use of MAC-ELISA cross-reactivity alone was more problematic...
Nucleic acid amplification assays for detection of La Crosse virus RNAAmy J Lambert
Division of Vector Borne Infectious Diseases, National Centers for Infectious Diseases, CDC, Rampart Rd, Fort Collins, CO 80521, USA
J Clin Microbiol 43:1885-9. 2005..The NASBA and quantitative real-time RT-PCR assays detect LAC viral RNA in a sensitive, specific, and rapid manner; these findings support the use of these assays in surveillance and diagnostic laboratory systems...
West Nile virus epizootiology in the southeastern United States, 2001Marvin S Godsey
Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
Vector Borne Zoonotic Dis 5:82-9. 2005..The primary transmission cycle of WNV in the southeastern United States apparently involves Culex mosquitoes and passerine birds. Chickens are frequently infected and may serve as effective sentinels in this region...
West Nile virusGrant L Campbell
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, Department of Health and Human Services, Fort Collins, Colorado, USA
Lancet Infect Dis 2:519-29. 2002..Serology remains the mainstay of laboratory diagnosis. No WN virus-specific treatment or vaccine is available. Prevention depends on organised, sustained vector mosquito control, and public education...
Duplex microsphere-based immunoassay for detection of anti-West Nile virus and anti-St. Louis encephalitis virus immunoglobulin m antibodiesAlison J Johnson
Division of Vector Borne Infectious Diseases, Centers for Disease Control DVBID, P O Box 2087, Fort Collins, CO 80522, USA
Clin Diagn Lab Immunol 12:566-74. 2005..The duplex MIA results compared favorably to those of the plaque-reduction neutralization test and MAC-ELISA. The assay proved to be reproducible, produced accurate classifications as to the infecting virus, and was specific...
O'Nyong-nyong fever in West AfricaDrew L Posey
Centers for Disease Control and Prevention, Division of Global Migration and Quarantine, Atlanta, Georgia 30333, USA
Am J Trop Med Hyg 73:32. 2005
Detection of West Nile virus-infected mosquitoes and seropositive juvenile birds in the vicinity of virus-positive dead birdsRoger S Nasci
Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
Am J Trop Med Hyg 67:492-6. 2002..In addition, the RT-PCR procedure for detecting WN viral RNA in mosquito pools detected more positive pools than did the Vero cell plaque assay...
Detection of Colorado tick fever virus by using reverse transcriptase PCR and application of the technique in laboratory diagnosisA J Johnson
Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA
J Clin Microbiol 35:1203-8. 1997..The reverse transcriptase PCR method is a promising tool for the early diagnosis of CTF viral infection, or for ruling out CTF virus as the etiologic agent, in order to facilitate appropriate medical support...
Virus and antibody dynamics in acute west nile virus infectionMichael P Busch
Blood Systems Research Institute, San Francisco, CA 94118, USA
J Infect Dis 198:984-93. 2008..The dynamics of the early stages of West Nile virus (WNV) infection can be assessed by follow-up studies of viremic blood donors...
Dengue viremia in blood donors from Honduras, Brazil, and AustraliaJeffrey M Linnen
Gen Probe, Inc, San Diego, California, USA
Transfusion 48:1355-62. 2008..Documented transfusion transmission of West Nile virus raised concern regarding transfusion-transmitted DENV...
Phylogenetic analysis of North American West Nile virus isolates, 2001-2004: evidence for the emergence of a dominant genotypeC Todd Davis
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, and Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77550-0609, USA
Virology 342:252-65. 2005..The implications of these findings are discussed as they relate to transmission and spread of the virus in the Western Hemisphere...
Construction of yellow fever/St. Louis encephalitis chimeric virus and the use of chimeras as a diagnostic toolKonstantin V Pugachev
Acambis Inc, 38 Sidney Street, Cambridge, MA 02139, USA
Am J Trop Med Hyg 71:639-45. 2004..These chimeras have the potential for use as diagnostic reagents and vaccines against SLE and WN...
Interferon and interferon-induced chemokine expression is associated with control of acute viremia in West Nile virus-infected blood donorsLeslie H Tobler
University of California, San Francisco, CA 94118, USA
J Infect Dis 198:979-83. 2008..These findings suggest that IFN-mediated innate immunity plays a key role in initial control of WNV replication...
Ross River virus disease reemergence, Fiji, 2003-2004Philipp Klapsing
Montreal General Hospital, Montreal, Quebec, Canada
Emerg Infect Dis 11:613-5. 2005..This report suggests that Ross River virus is once again circulating in Fiji, where it apparently disappeared after causing an epidemic in 1979 to 1980...
