Research Topics
| D R KilpatrickSummaryAffiliation: Centers for Disease Control and Prevention Country: USA Publications
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Detail Information
Publications
Poliovirus serotype-specific VP1 sequencing primersDavid R Kilpatrick
Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Virol Methods 174:128-30. 2011....
Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residuesDavid R Kilpatrick
Polio and Picornavirus Laboratory Branch, G 10, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Clin Microbiol 47:1939-41. 2009..Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates...
Multiplex PCR method for identifying recombinant vaccine-related poliovirusesDavid R Kilpatrick
Respiratory and Enteric Virus Branch, G 10, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Clin Microbiol 42:4313-5. 2004..Six pairs of Sabin strain-specific recombinant primers were designed to permit rapid screening for VDPV recombinants by PCR...
Type-specific detection of echovirus 30 isolates using degenerate reverse transcriptase PCR primersD R Kilpatrick
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Clin Microbiol 39:1299-302. 2001..No specific products were obtained by PCR assays containing templates from any of the other 63 EV serotypes. Inosine-containing degenerate primers may be widely applicable to the identification of echovirus serotypes by PCR...
Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracyD R Kilpatrick
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Center for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Clin Microbiol 34:2990-6. 1996..These degenerate PCR primers should aid in the detection of all polioviruses, including those wild poliovirus isolates for which genotype-specific reagents are unavailable...
Typing of human enteroviruses by partial sequencing of VP1M S Oberste
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Clin Microbiol 37:1288-93. 1999..The technique may also be useful for the rapid determination of whether viruses isolated during an outbreak are epidemiologically related...
Serotype-specific identification of enterovirus 71 by PCRB A Brown
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Clin Virol 16:107-12. 2000..Because enterovirus 71 is more often associated with severe neurological disease, there is a need to rapidly discriminate between enterovirus 71 and coxsackievirus A16 during hand, foot, and mouth disease outbreaks...
Serotype-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residues at positions of codon degeneracyD R Kilpatrick
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Clin Microbiol 36:352-7. 1998..These primers are useful for the rapid screening of poliovirus isolates and for determining the compositions of cultures containing mixtures of poliovirus serotypes...
Comparison of classic and molecular approaches for the identification of untypeable enterovirusesM S Oberste
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
J Clin Microbiol 38:1170-4. 2000..RT-PCR, coupled with amplicon sequencing, is a simple and rapid method for the typing and classification of picornaviruses and may lead to the identification of many new picornavirus serotypes...
An echovirus type 33 winter outbreak in New ZealandQ Sue Huang
Institute of Environmental Science and Research, Porirua, Wellington, New Zealand
Clin Infect Dis 37:650-7. 2003..The isolates were closely related to strains from Australia and Oman. Molecular typing, together with a serotype-specific E33 PCR, improved the speed and effectiveness of the outbreak investigation...
Emergence of echovirus type 13 as a prominent enterovirusJames A Mullins
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Clin Infect Dis 38:70-7. 2004..Continued surveillance of enteroviruses is important to alert physicians and public health officials to changes in disease trends and to improve efficiencies of clinical intervention...
Molecular epidemiology and type-specific detection of echovirus 11 isolates from the Americas, Europe, Africa, Australia, southern Asia and the Middle EastM Steven Oberste
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop G 17, Atlanta, GA 30333, USA
Virus Res 91:241-8. 2003..The aligned sequences were used to develop an E11-specific RT-PCR assay, using degenerate, inosine-containing primers, to amplify all members of all genogroups...
Acute flaccid paralysis from echovirus type 33 infectionKeith Grimwood
Department of Paediatrics and Child Health, Wellington School of Medicine and Health Sciences, New Zealand
J Clin Microbiol 41:2230-2. 2003..This is the first time an association between echovirus type 33 infection and acute flaccid paralysis has been reported...
