Justin M Hettick

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. ncbi request reprint Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
    Anal Biochem 380:276-81. 2008
  2. pmc Vapor conjugation of toluene diisocyanate to specific lysines of human albumin
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
    Anal Biochem 421:706-11. 2012
  3. doi request reprint Determination of the toluene diisocyanate binding sites on human serum albumin by tandem mass spectrometry
    Justin M Hettick
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
    Anal Biochem 414:232-8. 2011
  4. ncbi request reprint Structural elucidation of isocyanate-peptide adducts using tandem mass spectrometry
    Justin M Hettick
    Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    J Am Soc Mass Spectrom 20:1567-75. 2009
  5. ncbi request reprint Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505, USA
    Rapid Commun Mass Spectrom 22:2555-60. 2008
  6. ncbi request reprint Discrimination of intact mycobacteria at the strain level: a combined MALDI-TOF MS and biostatistical analysis
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV, USA
    Proteomics 6:6416-25. 2006
  7. pmc Characterization of methylene diphenyl diisocyanate-haptenated human serum albumin and hemoglobin
    Morgen Mhike
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Anal Biochem 440:197-204. 2013
  8. pmc Monoclonal antibodies to hyphal exoantigens derived from the opportunistic pathogen Aspergillus terreus
    Ajay P Nayak
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
    Clin Vaccine Immunol 18:1568-76. 2011
  9. doi request reprint Production, characterization and utility of a panel of monoclonal antibodies for the detection of toluene diisocyanate haptenated proteins
    Tinashe B Ruwona
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    J Immunol Methods 373:127-35. 2011
  10. ncbi request reprint Zinc diethyldithiocarbamate allergenicity: potential haptenation mechanisms
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 2888, USA
    Contact Dermatitis 59:79-89. 2008

Collaborators

Detail Information

Publications21

  1. ncbi request reprint Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
    Anal Biochem 380:276-81. 2008
    ..These data indicate that MALDI-TOF MS data may be used for unambiguous identification of members of the genus Aspergillus at both the species and strain levels...
  2. pmc Vapor conjugation of toluene diisocyanate to specific lysines of human albumin
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
    Anal Biochem 421:706-11. 2012
    ....
  3. doi request reprint Determination of the toluene diisocyanate binding sites on human serum albumin by tandem mass spectrometry
    Justin M Hettick
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
    Anal Biochem 414:232-8. 2011
    ..Diisocyanate-protein conjugation results in a variety of reaction products, including intra- and intermolecular crosslinking, diisocyanate self-polymerization, and diisocyanate hydrolysis...
  4. ncbi request reprint Structural elucidation of isocyanate-peptide adducts using tandem mass spectrometry
    Justin M Hettick
    Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    J Am Soc Mass Spectrom 20:1567-75. 2009
    ..The direct observation of isocyanate binding to the N-terminus of peptides under these experimental conditions is in good agreement with previous studies on the relative reaction rate of isocyanate with amino acid functional groups...
  5. ncbi request reprint Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505, USA
    Rapid Commun Mass Spectrom 22:2555-60. 2008
    ..e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance...
  6. ncbi request reprint Discrimination of intact mycobacteria at the strain level: a combined MALDI-TOF MS and biostatistical analysis
    Justin M Hettick
    Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV, USA
    Proteomics 6:6416-25. 2006
    ..023 using only 18 m/z variables. In addition, using random forest analysis of MALDI-TOF MS data, it was possible to correctly classify bacterial strains as either M. tuberculosis or non-tuberculous with 100% accuracy...
  7. pmc Characterization of methylene diphenyl diisocyanate-haptenated human serum albumin and hemoglobin
    Morgen Mhike
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Anal Biochem 440:197-204. 2013
    ....
  8. pmc Monoclonal antibodies to hyphal exoantigens derived from the opportunistic pathogen Aspergillus terreus
    Ajay P Nayak
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
    Clin Vaccine Immunol 18:1568-76. 2011
    ..Studies using confocal laser scanning microscopy showed that the uncharacterized leucine aminopeptidase mostly localized to extracellular matrix structures while dipeptidyl-peptidase V was mostly confined to the cytoplasm...
  9. doi request reprint Production, characterization and utility of a panel of monoclonal antibodies for the detection of toluene diisocyanate haptenated proteins
    Tinashe B Ruwona
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    J Immunol Methods 373:127-35. 2011
    ..Preliminary studies using TDI vapor exposed cells suggest potential utility of these mAbs for both research and biomonitoring...
  10. ncbi request reprint Zinc diethyldithiocarbamate allergenicity: potential haptenation mechanisms
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 2888, USA
    Contact Dermatitis 59:79-89. 2008
    ..Zinc diethyldithiocarbamate (ZDEC) and its disulfide, tetraethylthiuram disulfide (TETD), are rubber accelerators and contact allergens that cross-react in some individuals...
  11. doi request reprint Fungal pigments inhibit the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of darkly pigmented fungi
    Amanda D Buskirk
    National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
    Anal Biochem 411:122-8. 2011
    ..The addition of tricyclazole to the fungal growth media blocks fungal melanin synthesis and results in less melanized fungi that may be analyzed by MALDI-TOF MS...
  12. doi request reprint A murine monoclonal antibody with broad specificity for occupationally relevant diisocyanates
    Angela R Lemons
    a Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, West Virginia
    J Occup Environ Hyg 11:101-10. 2014
    ..The mAb may have additional utility in the standardization of specific IgE detection immunoassays as well as chromatographic-mass spectrometric methods to enrich dNCO adducted HSA in the plasma of occupationally exposed workers. ..
  13. doi request reprint Pulmonary immune responses to Aspergillus fumigatus in an immunocompetent mouse model of repeated exposures
    Amanda D Buskirk
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, USA
    J Immunotoxicol 11:180-9. 2014
    ..These data suggest that the presence of fungal melanin may modulate the pulmonary immune response in a mouse model of repeated exposures to A. fumigatus conidia...
  14. ncbi request reprint Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Justin M Hettick
    National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
    Anal Chem 76:5769-76. 2004
    ..This study demonstrates the potential for mass spectrometry-based identification/classification of mycobacteria...
  15. pmc Characterization of Cannabis sativa allergens
    Ajay P Nayak
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, West Virginia 26505, USA
    Ann Allergy Asthma Immunol 111:32-7. 2013
    ..To date, little is known about the causal allergens associated with C sativa...
  16. doi request reprint Characterization of recombinant terrelysin, a hemolysin of Aspergillus terreus
    Ajay P Nayak
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Rd, Morgantown, WV 26505, USA
    Mycopathologia 171:23-34. 2011
    ..Polyclonal antibody to rTerrelysin recognized a protein of approximately 16.5 kDa in mycelial extracts from A. terreus...
  17. ncbi request reprint Oxidation of 2-mercaptobenzothiazole in latex gloves and its possible haptenation pathway
    Itai Chipinda
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
    Chem Res Toxicol 20:1084-92. 2007
    ..The data suggest that the critical functional group on MBT is the thiol, and haptenation is via the formation of mixed disulfides between the thiol group on MBT and a protein sulfhydryl group...
  18. doi request reprint Analytical methodology and assessment of potential second-hand exposure to fentanyl in the hospital surgical setting
    Brandon F Law
    Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    J Addict Dis 29:51-8. 2010
    ..No fentanyl was detected in the air during cardiovascular surgical operations from either surgical suite...
  19. pmc Haptenation: chemical reactivity and protein binding
    Itai Chipinda
    Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, MS L 2040, 1095 Willowdale Road, Morgantown, WV 26505, USA
    J Allergy (Cairo) 2011:839682. 2011
    ..Chemical aspects related to both reactivity and protein-specific binding are discussed...
  20. doi request reprint Tissue binding affects the kinetics of theophylline diffusion through the stratum corneum barrier layer of skin
    H Frederick Frasch
    Health Effects Laboratory, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    J Pharm Sci 100:2989-95. 2011
    ..4. The permeability coefficient for TH is 4.86 × 10(-5) cm/h, and the lag time is 20.1 h. Binding occurs as a slow process, significantly affecting the kinetics of dermal penetration...
  21. ncbi request reprint Characterization of nigerlysin, hemolysin produced by Aspergillus niger, and effect on mouse neuronal cells in vitro
    Maura Donohue
    National Exposure Research Laboratory, U S Environmental Protection Agency, Cincinnati, OH, USA
    Toxicology 219:150-5. 2006
    ..1 microg ml(-1) of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24 h. The IC50 is estimated to be 0.037 microg ml(-1), or between 0.034 and 0.041 microg ml(-1) at the 95% confidence level...