Research Topics
| Justin M HettickSummaryAffiliation: Centers for Disease Control and Prevention Country: USA Publications
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Detail Information
Publications
Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprintingJustin M Hettick
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
Anal Biochem 380:276-81. 2008..These data indicate that MALDI-TOF MS data may be used for unambiguous identification of members of the genus Aspergillus at both the species and strain levels...
Vapor conjugation of toluene diisocyanate to specific lysines of human albuminJustin M Hettick
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA
Anal Biochem 421:706-11. 2012....
Determination of the toluene diisocyanate binding sites on human serum albumin by tandem mass spectrometryJustin M Hettick
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
Anal Biochem 414:232-8. 2011..Diisocyanate-protein conjugation results in a variety of reaction products, including intra- and intermolecular crosslinking, diisocyanate self-polymerization, and diisocyanate hydrolysis...
Structural elucidation of isocyanate-peptide adducts using tandem mass spectrometryJustin M Hettick
Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
J Am Soc Mass Spectrom 20:1567-75. 2009..The direct observation of isocyanate binding to the N-terminus of peptides under these experimental conditions is in good agreement with previous studies on the relative reaction rate of isocyanate with amino acid functional groups...
Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprintingJustin M Hettick
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505, USA
Rapid Commun Mass Spectrom 22:2555-60. 2008..e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance...
Discrimination of intact mycobacteria at the strain level: a combined MALDI-TOF MS and biostatistical analysisJustin M Hettick
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV, USA
Proteomics 6:6416-25. 2006..023 using only 18 m/z variables. In addition, using random forest analysis of MALDI-TOF MS data, it was possible to correctly classify bacterial strains as either M. tuberculosis or non-tuberculous with 100% accuracy...
Monoclonal antibodies to hyphal exoantigens derived from the opportunistic pathogen Aspergillus terreusAjay P Nayak
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
Clin Vaccine Immunol 18:1568-76. 2011..Studies using confocal laser scanning microscopy showed that the uncharacterized leucine aminopeptidase mostly localized to extracellular matrix structures while dipeptidyl-peptidase V was mostly confined to the cytoplasm...
Production, characterization and utility of a panel of monoclonal antibodies for the detection of toluene diisocyanate haptenated proteinsTinashe B Ruwona
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
J Immunol Methods 373:127-35. 2011..Preliminary studies using TDI vapor exposed cells suggest potential utility of these mAbs for both research and biomonitoring...
Zinc diethyldithiocarbamate allergenicity: potential haptenation mechanismsItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 2888, USA
Contact Dermatitis 59:79-89. 2008..Zinc diethyldithiocarbamate (ZDEC) and its disulfide, tetraethylthiuram disulfide (TETD), are rubber accelerators and contact allergens that cross-react in some individuals...
Fungal pigments inhibit the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of darkly pigmented fungiAmanda D Buskirk
National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
Anal Biochem 411:122-8. 2011..The addition of tricyclazole to the fungal growth media blocks fungal melanin synthesis and results in less melanized fungi that may be analyzed by MALDI-TOF MS...
Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometryJustin M Hettick
National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
Anal Chem 76:5769-76. 2004..This study demonstrates the potential for mass spectrometry-based identification/classification of mycobacteria...
Characterization of recombinant terrelysin, a hemolysin of Aspergillus terreusAjay P Nayak
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Rd, Morgantown, WV 26505, USA
Mycopathologia 171:23-34. 2011..Polyclonal antibody to rTerrelysin recognized a protein of approximately 16.5 kDa in mycelial extracts from A. terreus...
Oxidation of 2-mercaptobenzothiazole in latex gloves and its possible haptenation pathwayItai Chipinda
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505 2888, USA
Chem Res Toxicol 20:1084-92. 2007..The data suggest that the critical functional group on MBT is the thiol, and haptenation is via the formation of mixed disulfides between the thiol group on MBT and a protein sulfhydryl group...
Haptenation: chemical reactivity and protein bindingItai Chipinda
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, MS L 2040, 1095 Willowdale Road, Morgantown, WV 26505, USA
J Allergy (Cairo) 2011:839682. 2011..Chemical aspects related to both reactivity and protein-specific binding are discussed...
Analytical methodology and assessment of potential second-hand exposure to fentanyl in the hospital surgical settingBrandon F Law
Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
J Addict Dis 29:51-8. 2010..No fentanyl was detected in the air during cardiovascular surgical operations from either surgical suite...
Tissue binding affects the kinetics of theophylline diffusion through the stratum corneum barrier layer of skinH Frederick Frasch
Health Effects Laboratory, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
J Pharm Sci 100:2989-95. 2011..4. The permeability coefficient for TH is 4.86 × 10(-5) cm/h, and the lag time is 20.1 h. Binding occurs as a slow process, significantly affecting the kinetics of dermal penetration...
Characterization of nigerlysin, hemolysin produced by Aspergillus niger, and effect on mouse neuronal cells in vitroMaura Donohue
National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH, USA
Toxicology 219:150-5. 2006..1 microg ml(-1) of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24 h. The IC50 is estimated to be 0.037 microg ml(-1), or between 0.034 and 0.041 microg ml(-1) at the 95% confidence level...
