Affiliation: Centers for Disease Control and Prevention
Dauphin L, Stephens K, Eufinger S, Bowen M. Comparison of five commercial DNA extraction kits for the recovery of Yersinia pestis DNA from bacterial suspensions and spiked environmental samples. J Appl Microbiol. 2010;108:163-72 pubmed publisher
..The results of this study can assist diagnostic laboratories with selecting the best extraction method for processing environmental specimens for subsequent detection of Y. pestis by real-time PCR. ..
Dauphin L, Hutchins R, Bost L, Bowen M. Evaluation of automated and manual commercial DNA extraction methods for recovery of Brucella DNA from suspensions and spiked swabs. J Clin Microbiol. 2009;47:3920-6 pubmed publisher
Dauphin L, Moser B, Bowen M. Evaluation of five commercial nucleic acid extraction kits for their ability to inactivate Bacillus anthracis spores and comparison of DNA yields from spores and spiked environmental samples. J Microbiol Methods. 2009;76:30-7 pubmed publisher
..anthracis spores as well as DNA yield and purity. In addition, the extraction method used can influence the sensitivity of real-time PCR assays for B. anthracis. ..
Dauphin L, Walker R, Petersen J, Bowen M. Comparative evaluation of automated and manual commercial DNA extraction methods for detection of Francisella tularensis DNA from suspensions and spiked swabs by real-time polymerase chain reaction. Diagn Microbiol Infect Dis. 2011;70:299-306 pubmed publisher
..Furthermore, the results provide diagnostic laboratories with the option to select from 2 automated DNA extraction methods as suitable alternatives to manual methods for the isolation of DNA from F. tularensis. ..