L A Dauphin

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. doi request reprint A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations
    Leslie A Dauphin
    Division of Bioterrorism Preparedness and Response DBPR, National Center for Preparedness, Detection, and Control of Infectious Diseases NCPDCID, CDC, Atlanta, Georgia, USA
    J Microbiol Methods 76:212-4. 2009
  2. doi request reprint Comparison of five commercial DNA extraction kits for the recovery of Yersinia pestis DNA from bacterial suspensions and spiked environmental samples
    L A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, Division of Bioterrorism Preparedness and Response DBPR, National Center for Preparedness, Detection, and Control of Infectious Diseases NCPDCID, Centers for Disease Control and Prevention CDC, Atlanta, GA, USA
    J Appl Microbiol 108:163-72. 2010
  3. pmc Evaluation of automated and manual commercial DNA extraction methods for recovery of Brucella DNA from suspensions and spiked swabs
    Leslie A Dauphin
    Division of Bioterrorism Preparedness and Response, Bioterrorism Rapid Response and Advanced Technology Laboratory, National Center for Preparedness, Detection, and Control of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 47:3920-6. 2009
  4. pmc Swab protocol for rapid laboratory diagnosis of cutaneous anthrax
    Leslie A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, Division of Preparedness and Emerging Infections DPEI, National Center for Emerging and Zoonotic Infectious Diseases NCEZID, Centers for Disease Control and Prevention CDC, Atlanta, Georgia, USA
    J Clin Microbiol 50:3960-7. 2012
  5. doi request reprint Evaluation of five commercial nucleic acid extraction kits for their ability to inactivate Bacillus anthracis spores and comparison of DNA yields from spores and spiked environmental samples
    Leslie A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, National Center for Preparedness, Atlanta, Georgia 30333, USA
    J Microbiol Methods 76:30-7. 2009
  6. doi request reprint Comparative evaluation of automated and manual commercial DNA extraction methods for detection of Francisella tularensis DNA from suspensions and spiked swabs by real-time polymerase chain reaction
    Leslie A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, Laboratory Preparedness and Response Branch LPRB, Division of Preparedness and Emerging Infections DPEI, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Diagn Microbiol Infect Dis 70:299-306. 2011

Detail Information

Publications6

  1. doi request reprint A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations
    Leslie A Dauphin
    Division of Bioterrorism Preparedness and Response DBPR, National Center for Preparedness, Detection, and Control of Infectious Diseases NCPDCID, CDC, Atlanta, Georgia, USA
    J Microbiol Methods 76:212-4. 2009
    ..Centrifugal filtration with 0.1-microm filter units can be used following extraction of DNA from B. anthracis spores to render samples safe without compromising the sensitivity of diagnostic real-time PCR assays for B. anthracis...
  2. doi request reprint Comparison of five commercial DNA extraction kits for the recovery of Yersinia pestis DNA from bacterial suspensions and spiked environmental samples
    L A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, Division of Bioterrorism Preparedness and Response DBPR, National Center for Preparedness, Detection, and Control of Infectious Diseases NCPDCID, Centers for Disease Control and Prevention CDC, Atlanta, GA, USA
    J Appl Microbiol 108:163-72. 2010
    ..To evaluate commercial DNA extraction kits for their ability to isolate DNA from Yersinia pestis suspensions and spiked environmental samples...
  3. pmc Evaluation of automated and manual commercial DNA extraction methods for recovery of Brucella DNA from suspensions and spiked swabs
    Leslie A Dauphin
    Division of Bioterrorism Preparedness and Response, Bioterrorism Rapid Response and Advanced Technology Laboratory, National Center for Preparedness, Detection, and Control of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Clin Microbiol 47:3920-6. 2009
    ....
  4. pmc Swab protocol for rapid laboratory diagnosis of cutaneous anthrax
    Leslie A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, Division of Preparedness and Emerging Infections DPEI, National Center for Emerging and Zoonotic Infectious Diseases NCEZID, Centers for Disease Control and Prevention CDC, Atlanta, Georgia, USA
    J Clin Microbiol 50:3960-7. 2012
    ..anthracis from swabs at ≥ 7 days. Thus, we recommend the use of the SETS method with subsequent testing by culture and real-time PCR for diagnosis of cutaneous anthrax from clinical swabs of cutaneous lesions...
  5. doi request reprint Evaluation of five commercial nucleic acid extraction kits for their ability to inactivate Bacillus anthracis spores and comparison of DNA yields from spores and spiked environmental samples
    Leslie A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, National Center for Preparedness, Atlanta, Georgia 30333, USA
    J Microbiol Methods 76:30-7. 2009
    ..anthracis spores as well as DNA yield and purity. In addition, the extraction method used can influence the sensitivity of real-time PCR assays for B. anthracis...
  6. doi request reprint Comparative evaluation of automated and manual commercial DNA extraction methods for detection of Francisella tularensis DNA from suspensions and spiked swabs by real-time polymerase chain reaction
    Leslie A Dauphin
    Bioterrorism Rapid Response and Advanced Technology BRRAT Laboratory, Laboratory Preparedness and Response Branch LPRB, Division of Preparedness and Emerging Infections DPEI, Centers for Disease Control and Prevention CDC, Atlanta, GA 30333, USA
    Diagn Microbiol Infect Dis 70:299-306. 2011
    ..Furthermore, the results provide diagnostic laboratories with the option to select from 2 automated DNA extraction methods as suitable alternatives to manual methods for the isolation of DNA from F. tularensis...