Gregory Dasch

Summary

Affiliation: Centers for Disease Control
Location: Atlanta, GA, USA
Summary:
Genomics, Genetics, Diagnosis, Proteomics, Disease Ecology, Immunology and Microbiology of Rickettsia and Bartonella species and the diseases they cause

Publications

  1. pmc A focus of dogs and Rickettsia massiliae-infected Rhipicephalus sanguineus in California
    Emily Beeler
    Rickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Trop Med Hyg 84:244-9. 2011
  2. ncbi request reprint Fatal case of Brazilian spotted fever confirmed by immunohistochemical staining and sequencing methods on fixed tissues
    Tatiana Rozental
    Lab de Hantaviroses e Rickettsioses, Depto Virologia, Pav Rocha Lima 5 andar, Fiocruz Av Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, Brazil CEP 21045 900
    Ann N Y Acad Sci 1078:257-9. 2006
  3. ncbi request reprint A spotted fever group Rickettsia from an exotic tick species, Amblyomma exornatum (Acari: Ixodidae), in a reptile breeding facility in the United States
    Will K Reeves
    Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS G 13, Atlanta, GA 30333, USA
    J Med Entomol 43:1099-101. 2006
  4. ncbi request reprint Surveillance of Egyptian fleas for agents of public health significance: Anaplasma, Bartonella, Coxiella, Ehrlichia, Rickettsia, and Yersinia pestis
    Amanda D Loftis
    Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
    Am J Trop Med Hyg 75:41-8. 2006
  5. ncbi request reprint Louse-borne bacterial pathogens in lice (Phthiraptera) of rodents and cattle from Egypt
    Will K Reeves
    Centers for Disease Control and Prevention 1600 Clifton Rd NE, MS G 13, Atlanta, Georgia 30333, USA
    J Parasitol 92:313-8. 2006
  6. ncbi request reprint Rickettsial agents from parasitic dermanyssoidea (Acari: Mesostigmata)
    Will K Reeves
    Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS G 13, Atlanta, GA 30333, USA
    Exp Appl Acarol 38:181-8. 2006
  7. ncbi request reprint High-throughput molecular testing of ticks using a liquid-handling robot
    John R Moriarity
    Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Med Entomol 42:1063-7. 2005
  8. ncbi request reprint Acute hearing loss due to scrub typhus: a forgotten complication of a reemerging disease
    R Premaratna
    Department of Medicine, Faculty of Medicine, University of Kelaniya, Sri Lanka
    Clin Infect Dis 42:e6-8. 2006
  9. ncbi request reprint Detection of Rickettsia, Borrelia, and Bartonella in Carios kelleyi (Acari: Argasidae)
    Amanda D Loftis
    Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Med Entomol 42:473-80. 2005
  10. ncbi request reprint History of U.S. military contributions to the study of rickettsial diseases
    Mary F Bavaro
    Naval Medical Center San Diego, San Diego, CA 92134, USA
    Mil Med 170:49-60. 2005

Collaborators

Detail Information

Publications64

  1. pmc A focus of dogs and Rickettsia massiliae-infected Rhipicephalus sanguineus in California
    Emily Beeler
    Rickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Am J Trop Med Hyg 84:244-9. 2011
    ..massiliae as the most likely spotted fever group rickettsiae responsible for seropositivity. To our knowledge, this is the first detection of R. massiliae in ticks in California...
  2. ncbi request reprint Fatal case of Brazilian spotted fever confirmed by immunohistochemical staining and sequencing methods on fixed tissues
    Tatiana Rozental
    Lab de Hantaviroses e Rickettsioses, Depto Virologia, Pav Rocha Lima 5 andar, Fiocruz Av Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, Brazil CEP 21045 900
    Ann N Y Acad Sci 1078:257-9. 2006
    ..The authors describe the first characterization of Rickettsia rickettsii in a fatal case occurring in Rio de Janeiro State, Brazil...
  3. ncbi request reprint A spotted fever group Rickettsia from an exotic tick species, Amblyomma exornatum (Acari: Ixodidae), in a reptile breeding facility in the United States
    Will K Reeves
    Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS G 13, Atlanta, GA 30333, USA
    J Med Entomol 43:1099-101. 2006
    ..The detection of a spotted fever group Rickettsia in exotic ticks emphasizes the potential threat posed by the importation and propagation of exotic animals in the United States...
  4. ncbi request reprint Surveillance of Egyptian fleas for agents of public health significance: Anaplasma, Bartonella, Coxiella, Ehrlichia, Rickettsia, and Yersinia pestis
    Amanda D Loftis
    Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
    Am J Trop Med Hyg 75:41-8. 2006
    ..cheopis. Novel Bartonella genotypes were detected in X. cheopis and L. segnis from three cities. Coxiella burnetii was detected in two fleas. Anaplasma, Ehrlichia, and Y. pestis were not detected...
  5. ncbi request reprint Louse-borne bacterial pathogens in lice (Phthiraptera) of rodents and cattle from Egypt
    Will K Reeves
    Centers for Disease Control and Prevention 1600 Clifton Rd NE, MS G 13, Atlanta, Georgia 30333, USA
    J Parasitol 92:313-8. 2006
    ..More lice from governorates bordering the Mediterranean and Red Seas contained pathogens. Our data indicate that lice of urban and domestic animals harbor pathogenic or potentially pathogenic bacterial agents throughout Egypt...
  6. ncbi request reprint Rickettsial agents from parasitic dermanyssoidea (Acari: Mesostigmata)
    Will K Reeves
    Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS G 13, Atlanta, GA 30333, USA
    Exp Appl Acarol 38:181-8. 2006
    ..a novel Bartonella sp., Spiroplasma sp., Wolbachia sp., and an unclassified Rickettsiales were detected in mites. These could represent mite-borne bacterial agents, bacterial DNA from blood meals, or novel endosymbionts of mites...
  7. ncbi request reprint High-throughput molecular testing of ticks using a liquid-handling robot
    John R Moriarity
    Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Med Entomol 42:1063-7. 2005
    ..038 microl when dispensing 1.0 microl. By using this process, 96 samples can be extracted and tested while reducing human labor to approximately 30 min...
  8. ncbi request reprint Acute hearing loss due to scrub typhus: a forgotten complication of a reemerging disease
    R Premaratna
    Department of Medicine, Faculty of Medicine, University of Kelaniya, Sri Lanka
    Clin Infect Dis 42:e6-8. 2006
    ..Acute hearing loss or hearing impairment in a febrile patient should arouse strong suspicion of scrub typhus...
  9. ncbi request reprint Detection of Rickettsia, Borrelia, and Bartonella in Carios kelleyi (Acari: Argasidae)
    Amanda D Loftis
    Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Med Entomol 42:473-80. 2005
    ..turicatae, was detected in one of 31 live ticks. The 16S-23S intergenic spacer region of Bartonella henselae also was detected in one of 31 live ticks. Coxiella or A. phagocytophilum DNA were not detected in these ticks...
  10. ncbi request reprint History of U.S. military contributions to the study of rickettsial diseases
    Mary F Bavaro
    Naval Medical Center San Diego, San Diego, CA 92134, USA
    Mil Med 170:49-60. 2005
    ..Several rickettsial diseases, including epidemic typhus, scrub typhus, the ehrlichioses, and the spotted fevers, are reviewed, with emphasis on the military historical significance and contributions...
  11. ncbi request reprint Evidence of Rickettsia typhi and the potential for murine typhus in Jayapura, Irian Jaya, Indonesia
    Allen L Richards
    U S Naval Medical Research Unit No 2, Jakarta, Indonesia
    Am J Trop Med Hyg 66:431-4. 2002
    ..typhi (11.0%). These data show for the first time that rodents exposed to R. typhi are well established in Jayapura, and that some of these rodents harbor fleas potentially capable of transmitting murine typhus and plague...
  12. ncbi request reprint Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combi
    J S Dumler
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA
    Int J Syst Evol Microbiol 51:2145-65. 2001
    ..nov. and the genus Neorickettsia should be emended to include Neorickettsia (Ehrlichia) risticii comb. nov. and Neorickettsia (Ehrlichia) sennetsu comb. nov...
  13. pmc Evaluation of a commercially available recombinant-protein enzyme-linked immunosorbent assay for detection of antibodies produced in scrub typhus rickettsial infections
    M V Land
    PanBio Pty Ltd, Windsor, Queensland, Australia
    J Clin Microbiol 38:2701-5. 2000
    ....
  14. ncbi request reprint Western blotting analysis of heat shock proteins of Rickettsiales and other eubacteria
    M E Eremeeva
    Department of Microbiology and Immunology, University of Maryland, School of Medicine, Baltimore 21201, USA
    FEMS Microbiol Lett 167:229-37. 1998
    ..coli Hsp are not suitable for detection and identification of homologues of these proteins in a wide range of eubacteria...
  15. ncbi request reprint Molecular typing of novel Rickettsia rickettsii isolates from Arizona
    Marina E Eremeeva
    Viral and Rickettsial Zoonoses Branch, CDC, Atlanta, GA 30333, USA
    Ann N Y Acad Sci 1078:573-7. 2006
    ..Two of the twelve VNTRs had differences in the number of repeat sequences in isolates from Arizona compared to those from Montana, thus conferring the novelty of the Rh. sanguineus-associated R. rickettsii...
  16. ncbi request reprint Rickettsial pathogens in the tropical rat mite Ornithonyssus bacoti (Acari: Macronyssidae) from Egyptian rats (Rattus spp.)
    Will K Reeves
    Exp Appl Acarol 41:101-7. 2007
    ..were detected in eight pools of mites. Further research could demonstrate the vector potential of mites and pathogenicity of these agents to humans or animals...
  17. ncbi request reprint Infection of a goat with a tick-transmitted Ehrlichia from Georgia, U.S.A., that is closely related to Ehrlichia ruminantium
    Amanda D Loftis
    Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Mail Stop G 13, Atlanta, GA 30333, USA
    J Vector Ecol 31:213-23. 2006
    ..Our data suggest that A. americanum is a natural vector and reservoir of this Ehrlichia and that domestic goats can be reservoirs. The geographic range of the agent and its pathogenicity to humans and livestock needs to be evaluated...
  18. pmc Eschar-associated spotted fever rickettsiosis, Bahia, Brazil
    Nanci Silva
    Medicine and Public Health School of Bahia, Salvador, Brazil
    Emerg Infect Dis 17:275-8. 2011
    ..The etiologic agent is most related to Rickettsia parkeri, R. africae, and R. sibirica and probably widely distributed from Sao Paulo to Bahia in the Atlantic Forest...
  19. pmc Rickettsia felis, West Indies
    Patrick J Kelly
    Ross University School of Veterinary Medicine, St Kitts, West Indies P J Kelly, H Lucas Centers for Disease Control and Prevention, Atlanta, Georgia, USA M E Eremeeva, K G Dirks, G A Dasch Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Marseille, France J M Rolain, D Raoult University of Florida, Gainesville, Florida, USA C Yowell and Division of Agriculture, Roseau, Dominica, West Indies R Thomas, T Douglas
    Emerg Infect Dis 16:570-1. 2010
    ..The antibodies were detected with Rickettsia rickettsii antigen in a standard microimmunofluorescence assay (1). Titers for 13 (20%) cats were >/=320...
  20. ncbi request reprint Detection and identification of rickettsial agents in ticks from domestic mammals in eastern Panama
    Sergio E Bermúdez
    Instituto Conmemorativo Gorgas de Estudios de la Salud, Panama City, Panama 0816 02593
    J Med Entomol 46:856-61. 2009
    ....
  21. doi request reprint Comparative proteomic analysis of antibiotic-sensitive and insensitive isolates of Orientia tsutsugamushi
    Chien Chung Chao
    Viral and Rickettsial Diseases Department, Infectious Diseases Directorate, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Ann N Y Acad Sci 1166:27-37. 2009
    ..These data confirm the tremendous proteomic diversity of isolates of Orientia and suggest that drug insensitivity in this species may arise from multiple mechanisms...
  22. doi request reprint Closing the gaps between genotype and phenotype in Rickettsia rickettsii
    Marina E Eremeeva
    Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Ann N Y Acad Sci 1166:12-26. 2009
    ..Characterization of the physiologically important changes in rickettsial gene expression that occur immediately after tick-to-human or tick-to-animal transitions may require development of new experimental systems...
  23. doi request reprint Incongruent effects of two isolates of Rickettsia conorii on the survival of Rhipicephalus sanguineus ticks
    M L Levin
    Rickettsial Zoonoses Branch, Mail Stop G 13, National Center for Zoonotic, Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Exp Appl Acarol 49:347-59. 2009
    ..The vertebrate host may be a more important reservoir than previously thought, or co-feeding transmission between different generations of ticks may obviate or lessen the requirement for transovarial maintenance of R. conorii...
  24. ncbi request reprint Detection and identification of spotted fever group rickettsiae in Dermacentor species from southern California
    Mary E Wikswo
    National Center for Zoonotic, Vector Borne and Enteric Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
    J Med Entomol 45:509-16. 2008
    ..Because neither D. variabilis nor R. rickettsii were abundant, 364D should be evaluated further as a potential cause of human SFG rickettsioses in southern California...
  25. pmc On Rickettsia nomenclature
    Robert F Massung
    Centers for Disease Control and Prevention, 1600 Clifton Rd, Mailstop G13, Atlanta, GA 30333, USA
    Emerg Infect Dis 14:511. 2008
  26. ncbi request reprint Bartonella and Rickettsia from fleas (Siphonaptera: Ceratophyllidae) of prairie dogs (Cynomys spp.) from the western United States
    Will K Reeves
    J Parasitol 93:953-5. 2007
    ..and 1 Rickettsia sp. from 12 fleas collected in North Dakota, Oklahoma, Texas, and Wyoming. Sequences from the Bartonella spp. were similar, but not identical, to those from prairie dogs and their fleas in Colorado...
  27. ncbi request reprint Detection and identification of bacterial agents in Ixodes persulcatus Schulze ticks from the north western region of Russia
    Marina E Eremeeva
    Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Vector Borne Zoonotic Dis 7:426-36. 2007
    ..Since I. persulcatus is so commonly infected with multiple agents that may cause human diseases, exposure to these ticks poses significant risk to human health in this region...
  28. ncbi request reprint Bacteremia, fever, and splenomegaly caused by a newly recognized bartonella species
    Marina E Eremeeva
    Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 356:2381-7. 2007
    ..We characterized this isolate, including its ability to cause fever and sustained bacteremia in a rhesus macaque. The route of infection and burden of human disease associated with this newly described pathogen are currently unknown...
  29. ncbi request reprint Genotypic comparison of five isolates of Rickettsia prowazekii by multilocus sequence typing
    Hong Ge
    Rickettsial Diseases Department, Infectious Diseases Directorate, Naval Medical Research Center, MD 20910, USA
    FEMS Microbiol Lett 271:112-7. 2007
    ..Limited sequence diversity of ompB (0.02-0.11%) and sca4 (0.03-0.20%) was enough to distinguish the isolates, and evaluation of the combined four genes provided a method to easily differentiate R. prowazekii from other rickettsiae...
  30. ncbi request reprint Serologic survey of Eptesicus fuscus from Georgia, U.S.A. for Rickettsia and Borrelia and laboratory transmission of a Rickettsia by bat ticks
    Will K Reeves
    Centers for Disease Control and Prevention, 1600 Clifton Rd NE, MS G 13, Atlanta, GA 30333, USA
    J Vector Ecol 31:386-9. 2006
    ..fuscus is naturally exposed to both a spotted fever group Rickettsia and a relapsing fever group Borrelia. If these agents are transmitted by bat ticks, then people living in close proximity to bat ticks might be exposed...
  31. ncbi request reprint Genetic analysis of isolates of the spotted fever group of rickettsiae belonging to the R. conorii complex
    G A Dasch
    Viral and Rickettsial Diseases Program, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
    Ann N Y Acad Sci 849:11-20. 1998
    ..conorii complex isolates into three groups corresponding to the RC, RA, and ISTT rOmp serotypes, additional intragroup genetic variation was also detected in all three serotypes...
  32. pmc Expression and refolding of truncated recombinant major outer membrane protein antigen (r56) of Orientia tsutsugamushi and its use in enzyme-linked immunosorbent assays
    W M Ching
    Viral and Rickettsial Diseases Program, Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889 5607, USA
    Clin Diagn Lab Immunol 5:519-26. 1998
    ..These results strongly suggest that purified r56 is a suitable candidate for replacing the density gradient-purified, rickettsia-derived, whole-cell antigen currently used in the commercial dipstick assay available in the United States...
  33. ncbi request reprint Detection of murine typhus infected fleas with an enzyme-linked immunosorbent assay
    M E Dobson
    Rickettsial Diseases Division, Naval Medical Research Institute, Bethesda, Maryland
    Am J Trop Med Hyg 40:521-8. 1989
    ..7-6.9) found for pooled fleas in the same cohort. The sensitivity and ease of performance of ELISA should make it usable under field conditions...
  34. pmc Heterogeneity of CD4-positive human T-cell clones which recognize the surface protein antigen of Rickettsia typhi
    M Carl
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814 5055
    Infect Immun 57:1276-80. 1989
    ..We conclude that the SPAs from typhus group rickettsiae can elicit both a diverse T-cell response in humans and the efficient stimulation of gamma interferon-mediated immunity...
  35. pmc Mechanisms of immunity to infection with typhus rickettsiae: infected fibroblasts bear rickettsial antigens on their surfaces
    F M Rollwagen
    Infect Immun 50:911-6. 1985
    ..The significance of this finding in the pathophysiology of rickettsia-host interactions and the generation of cytotoxic T cell-mediated immunity and antibody immunity is discussed...
  36. ncbi request reprint Detection of rickettsiae in arthropod vectors by DNA amplification using the polymerase chain reaction
    A F Azad
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201
    Ann N Y Acad Sci 590:557-63. 1990
  37. ncbi request reprint Diagnosis of acute typhus infection using the polymerase chain reaction
    M Carl
    Naval Medical Research Institute, Naval Medical Command, Bethesda, Maryland 20814 5055
    Ann N Y Acad Sci 590:439-44. 1990
    ..The assay was able to detect as few as 30 rickettsiae. Detection of PCR-amplified DNA with a digoxigenin-labeled DNA probe confirmed an acute human infection with Rickettsia prowazekii...
  38. ncbi request reprint A structural and immunological comparison of rickettsial HSP60 antigens with those of other species
    G A Dasch
    Rickettsial Diseases Division, Naval Medical Research Institute, Bethesda, Maryland 20814 5055
    Ann N Y Acad Sci 590:352-69. 1990
  39. pmc Characterization of the gene encoding the protective paracrystalline-surface-layer protein of Rickettsia prowazekii: presence of a truncated identical homolog in Rickettsia typhi
    M Carl
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20814 5055
    Proc Natl Acad Sci U S A 87:8237-41. 1990
    ..prowazekii SPA but had a 3674-base-pair region identical with the remainder of the R. prowazekii SPA gene sequence...
  40. pmc Detection of murine typhus infection in fleas by using the polymerase chain reaction
    L Webb
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore
    J Clin Microbiol 28:530-4. 1990
    ..Like the ELISA, the PCR assay sensitivity was due in part to its suitability for detecting small numbers of both live and dead R. typhi in fleas...
  41. pmc Molecular cloning and sequence analysis of the Sta58 major antigen gene of Rickettsia tsutsugamushi: sequence homology and antigenic comparison of Sta58 to the 60-kilodalton family of stress proteins
    C K Stover
    Department of Rickettsial Diseases, Walter Reed Army Institute of Research, Washington, D C 20307
    Infect Immun 58:1360-8. 1990
    ..The antigenic uniqueness of the Sta58 antigen indicates that this protein may be a potentially protective antigen and a useful diagnostic reagent for scrub typhus fever...
  42. ncbi request reprint Diagnosis of acute typhus infection using the polymerase chain reaction
    M Carl
    Naval Medical Research Institute, Bethesda, Maryland 20814 5055
    J Infect Dis 161:791-3. 1990
    ..The assay could detect as few as 30 rickettsiae. Detection of PCR-amplified DNA with a nonradioactive DNA probe confirmed an acute infection with Rickettsia prowazekii...
  43. ncbi request reprint Mapping of monoclonal antibody binding sites on CNBr fragments of the S-layer protein antigens of Rickettsia typhi and Rickettsia prowazekii
    W M Ching
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20889 5055
    Mol Immunol 29:95-105. 1992
    ..Two types of antibodies bound to several CNBr fragments which contained both limited sequence similarity and modified amino acids either of which might account for the multisite binding of these antibodies...
  44. pmc Biochemical characteristics of typhus group rickettsiae with special attention to the Rickettsia prowazekii strains isolated from flying squirrels
    G A Dasch
    Infect Immun 19:676-85. 1978
    ..Despite these minor differences, a striking similarity was revealed by all the biochemical tests performed between the R. prowazekii strains of human and flying squirrel origin...
  45. pmc Sensitive enzyme-linked immunosorbent assay for detection of antibodies against typhus rickettsiae, Rickettsia prowazekii and Rickettsia typhi
    S Halle
    J Clin Microbiol 6:101-10. 1977
    ..ELISA titers showed some type specificity, whereas none was observed in complement fixation tests. The ELISA is highly sensitive, reproducible, and easily adaptable to the various requirements of clinical and research laboratories...
  46. pmc Sensitive microplate enzyme-linked immunosorbent assay for detection of antibodies against the scrub typhus rickettsia, Rickettsia tsutsugamushi
    G A Dasch
    J Clin Microbiol 9:38-48. 1979
    ..It is concluded that the ELISA for scrub typhus is a very satisfactory alternative to the IFA test...
  47. ncbi request reprint In vitro antigen-specific antibody response to the species-specific surface protein antigens of typhus group rickettsiae by human peripheral blood mononuclear cells: generation of an antigen-dependent suppressor T cell
    J Misiti
    J Immunol 134:2689-94. 1985
    ..This system should serve as an effective tool for analyzing the cellular interactions involved in the in vitro regulation of antigen-specific antibody synthesis by human PBMC...
  48. ncbi request reprint The line blot: an immunoassay for monoclonal and other antibodies. Its application to the serotyping of gram-negative bacteria
    D Raoult
    Centre National de Reference des Rickettsioses, C H U la Timone, Marseille, France
    J Immunol Methods 125:57-65. 1989
    ..The value of the line blot serotyping assay is demonstrated with Proteus. Rickettsia, Rochalimaea, and Legionella antigens. The line blot immunoassay is a simple, but powerful and flexible, alternative to dot and cross-dot immunoassays...
  49. ncbi request reprint Short report: surveillance of rickettsial infections in Indonesian military personnel during peace keeping operations in Cambodia
    A L Corwin
    U S Naval Medical Research Unit No 2, Jakarta, Indonesia
    Am J Trop Med Hyg 57:569-70. 1997
    ..Surveillance of rickettsial infections and/or disease is particularly warranted in Cambodia with recent recognition of drug-resistant scrub typhus in neighboring Thailand...
  50. ncbi request reprint Scrub typhus infections poorly responsive to antibiotics in northern Thailand
    G Watt
    Department of Medicine, US Army Component, AFRIMS, Bangkok, Thailand
    Lancet 348:86-9. 1996
    ....
  51. pmc Identification and characterization of epitopes on the 120-kilodalton surface protein antigen of Rickettsia prowazekii with synthetic peptides
    W M Ching
    Naval Medical Research Institute, Bethesda, Maryland, 20889 5607, USA
    Infect Immun 64:1413-9. 1996
    ....
  52. ncbi request reprint Canine ehrlichiosis in Egypt: sero-epidemiological survey
    B A Botros
    US Naval Medical Research Unit No 3, Cairo, Egypt
    Onderstepoort J Vet Res 62:41-3. 1995
    ..Three dogs with epistaxis had E. canis antibody titres > 1:320. These data demonstrate the first laboratory evidence of E. canis infection among dogs in Egypt...
  53. ncbi request reprint First cases of spotted fever group rickettsiosis in Thailand
    T Sirisanthana
    Faculty of Medicine, Chiang Mai University, Thailand
    Am J Trop Med Hyg 50:682-6. 1994
    ..All three patients responded to treatment with a single dose of doxycycline...
  54. pmc Isolation, cultivation, and partial characterization of the ELB agent associated with cat fleas
    S Radulovic
    Department of Microbiology and Immunology, School of Medicine, University of Baltimore, Maryland 21201 1559, USA
    Infect Immun 63:4826-9. 1995
    ..Coomassie blue-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of the ELB isolate and two R. typhi strains were identical...
  55. ncbi request reprint Comparative acute oral toxicity of para-aminopropiophenone (PAPP) in mammals and birds
    P J Savarie
    Bull Environ Contam Toxicol 30:122-6. 1983
  56. pmc Use of a sensitive microplate enzyme-linked immunosorbent assay in a retrospective serological analysis of a laboratory population at risk to infection with typhus group rickettsiae
    S Halle
    J Clin Microbiol 12:343-50. 1980
    ..The microplate ELISA proved to be a highly sensitive and reliable test for detection of the human serological response to typhus antigens...
  57. pmc Partial purification and characterization of the major species-specific protein antigens of Rickettsia typhi and Rickettsia prowazekii identified by rocket immunoelectrophoresis
    G A Dasch
    Infect Immun 31:276-88. 1981
    ..typhi reacted only very weakly with antiserum prepared against R. prowazekii according to the enzyme-linked immunosorbent assay and not at all according to rocket immunoelectrophoresis...
  58. pmc Recognition of typhus group rickettsia-infected targets by human lymphokine-activated killer cells
    M Carl
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814 5055
    Infect Immun 56:2526-9. 1988
    ..Major unaltered antigenic determinants of TGR therefore cannot be detected on the surface of infected targets...
  59. ncbi request reprint Lysis of cells infected with typhus group rickettsiae by a human cytotoxic T cell clone
    M Carl
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20892
    J Immunol 139:4203-7. 1987
    ..These results demonstrate, for the first time, that cells infected with intracellular bacteria can be lysed by human cytotoxic T lymphocytes...
  60. pmc Lymphokine-activated-killer-mediated lysis of cells infected with typhus group rickettsiae can be inhibited by OKT3 monoclonal antibody
    M Carl
    Infect Immun 53:226-8. 1986
    ..Lysis was significantly inhibited by OKT3 monoclonal antibody but not by OKT4 or OKT8 monoclonal antibody...
  61. ncbi request reprint Characterization of human cytotoxic lymphocytes directed against cells infected with typhus group rickettsiae: evidence for lymphokine activation of effectors
    M Carl
    J Immunol 136:2654-61. 1986
    ..We conclude that lysis of cells infected with typhus group rickettsiae is mediated by a lymphokine-activated killer...
  62. pmc Energy metabolism of monocytic Ehrlichia
    E Weiss
    Naval Medical Research Institute, Bethesda, MD 20814
    Proc Natl Acad Sci U S A 86:1674-8. 1989
    ....
  63. pmc Cluster of sylvatic epidemic typhus cases associated with flying squirrels, 2004-2006
    Alice S Chapman
    Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    Emerg Infect Dis 15:1005-11. 2009
    ..prowazekii. Education and control measures to exclude flying squirrels from housing are essential to reduce the likelihood of sylvatic epidemic typhus...
  64. pmc Clinically helpful rickettsial disease diagnostic IgG titers in relation to duration of illness in an endemic setting in Sri Lanka
    Ranjan Premaratna
    Department of Medicine, Faculty of Medicine, University of Kelaniya, Colombo, Sri Lanka
    BMC Res Notes 5:662. 2012
    ....