Affiliation: Centers for Disease Control and Prevention
- Detection of West Nile virus in formalin-fixed, paraffin-embedded human tissues by RT-PCR: a useful adjunct to conventional tissue-based diagnostic methodsJulu Bhatnagar
Infectious Disease Pathology Activity, Centers for Disease Control and Prevention, 1600 Clifton Road, N E, Mailstop G32, Atlanta, GA 30333, United States
J Clin Virol 38:106-11. 2007....
- Molecular detection and typing of dengue viruses from archived tissues of fatal cases by rt-PCR and sequencing: diagnostic and epidemiologic implicationsJulu Bhatnagar
Infectious Diseases Pathology Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Am J Trop Med Hyg 86:335-40. 2012..The IHC detected DENV antigens in 50 (40%) cases. The RT-PCR using FFPE tissue improves detection of DENV in fatal cases and provides sequence information useful for typing and epidemiologic studies...
- Localization of pandemic 2009 H1N1 influenza A virus RNA in lung and lymph nodes of fatal influenza cases by in situ hybridization: new insights on virus replication and pathogenesisJulu Bhatnagar
Infectious Diseases Pathology Branch, Division of High Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Clin Virol 56:232-7. 2013..Although pH1N1 viral antigens have been demonstrated in various human tissues by immunohistochemistry (IHC), cellular localization of pH1N1 RNA in these tissues has largely remained uninvestigated...
- Rapid, simultaneous detection of Clostridium sordellii and Clostridium perfringens in archived tissues by a novel PCR-based microsphere assay: diagnostic implications for pregnancy-associated toxic shock syndrome casesJulu Bhatnagar
Infectious Diseases Pathology Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Infect Dis Obstet Gynecol 2012:972845. 2012..The microsphere assay is a rapid, specific, and cost-effective method for the diagnosis of CTS and offers the advantage of simultaneous testing for C. sordellii and C. perfringens in FFPE tissues using a limited amount of DNA...
- Toxic shock associated with Clostridium sordellii and Clostridium perfringens after medical and spontaneous abortionAdam L Cohen
Prevention and Response Branch, Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Obstet Gynecol 110:1027-33. 2007..To better understand the risk of fatal toxic shock caused by Clostridium sordellii in women who had a recent medical abortion with mifepristone and misoprostol...
- Diagnosis of influenza from respiratory autopsy tissues: detection of virus by real-time reverse transcription-PCR in 222 casesAmy M Denison
Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
J Mol Diagn 13:123-8. 2011..We also determined that testing of all available respiratory tissues is critical for optimal detection of influenza virus in postmortem tissues...
- 2009 pandemic influenza A (H1N1): pathology and pathogenesis of 100 fatal cases in the United StatesWun Ju Shieh
Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, MS G 32, Atlanta, GA 30333, USA
Am J Pathol 177:166-75. 2010..Underlying medical conditions and bacterial co-infections contributed to the fatal outcome of this infection. More studies are needed to understand the multifactorial pathogenesis of this infection...
- Neutrophilic bacterial meningitis: pathology and etiologic diagnosis of fatal casesJeannette Guarner
Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA 30322, USA
Mod Pathol 26:1076-85. 2013..In summary, our study shows that S. pneumoniae continues to be the most frequent cause of fatal neutrophilic bacterial meningitis followed by N. meningitidis, both vaccine preventable diseases...
- Fatal Infectious Diseases during Pandemic (H1N1) 2009 OutbreakDianna M Blau
Centers for Disease Control and Prevention, Atlanta, GA, USA
Emerg Infect Dis 17:2069-70. 2011....
- Histopathologic, immunohistochemical, and polymerase chain reaction assays in the study of cases with fatal sporadic myocarditisJeannette Guarner
Infectious Disease Pathology Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Hum Pathol 38:1412-9. 2007..Accurately identifying an infectious agent is the foundation for clinical and public health interventions. Despite using multiple diagnostic methods, an organism could only be detected in a small proportion of sporadic myocarditis cases...
- Correlation of the detection of Clostridium difficile toxins in stools and presence of the clostridia in tissues of childrenJeannette Guarner
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
Hum Pathol 41:1586-92. 2010..Clostridial antigens were only observed with histopathologic evidence of pseudomembranes...
- Fatal hemorrhagic fever caused by West Nile virus in the United StatesChristopher D Paddock
Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
Clin Infect Dis 42:1527-35. 2006..We describe a case of fulminant WNV infection in a 59-year-old Florida man who died following a brief illness that resembled hemorrhagic disease caused by Rickettsia reckettsii, dengue virus or yellow fever virus...
- Cell culture and electron microscopy for identifying viruses in diseases of unknown causeCynthia S Goldsmith
Centers for Disease Control and Prevention, 1600 Clifton Rd NE, Mailstop G32, Atlanta, GA 30329, USA
Emerg Infect Dis 19:886-91. 2013..These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases...
- Fatal toxic shock syndrome associated with Clostridium sordellii after medical abortionMarc Fischer
Centers for Disease Control and Prevention, Atlanta, USA
N Engl J Med 353:2352-60. 2005..These cases indicate the need for physician awareness of this syndrome and for further study of its association with medical abortion...
- Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogensRobert Benson
CDC, Atlanta, GA 30333, USA
J Clin Microbiol 46:2074-7. 2008..The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction...
- Clostridium difficile-associated diarrhea: an emerging threat to pregnant womenNadine G Rouphael
Division of Bacterial Diseases, Epidemic Intelligence Service Field Assignments Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA
Am J Obstet Gynecol 198:635.e1-6. 2008..To estimate if Clostridium difficile-associated disease (CDAD) is increasing in peripartum women...
- Persistent neuroinvasive West Nile virus infection in an immunocompromised patientRobert G Penn
Department of Epidemiology, Methodist Hospital, Omaha, Nebraska, USA
Clin Infect Dis 42:680-3. 2006..This patient's case raises important issues related to the diagnosis, pathogenesis, and possible treatment of persistent WNV infection...