John R Barr

Summary

Affiliation: Centers for Disease Control and Prevention
Country: USA

Publications

  1. doi request reprint A rapid method for capture and identification of immunogenic proteins in Bordetella pertussis enriched membranes fractions: a fast-track strategy applicable to other microorganisms
    Rolieria West
    Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Proteomics 75:1966-72. 2012
  2. ncbi request reprint New high-resolution mass spectrometric approach for the measurement of polychlorinated biphenyls and organochlorine pesticides in human serum
    John R Barr
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Atlanta, GA 30341, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 794:137-48. 2003
  3. ncbi request reprint Analysis of urinary metabolites of sulfur mustard in two individuals after accidental exposure
    John R Barr
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
    J Anal Toxicol 32:10-6. 2008
  4. ncbi request reprint Quantitation of metabolites of the nerve agents sarin, soman, cyclohexylsarin, VX, and Russian VX in human urine using isotope-dilution gas chromatography-tandem mass spectrometry
    John R Barr
    Centers for Disease Control and Prevention, National Center for Environmental Health, 4770 Buford Highway NE, Mailstop F 47, Atlanta, Georgia 30341, USA
    J Anal Toxicol 28:372-8. 2004
  5. ncbi request reprint Measurement of toxaphene congeners in pooled human serum collected in three U.S. cities using high-resolution mass spectrometry
    John R Barr
    Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Arch Environ Contam Toxicol 46:551-6. 2004
  6. doi request reprint Quantitative mass spectrometry for bacterial protein toxins--a sensitive, specific, high-throughput tool for detection and diagnosis
    Anne E Boyer
    Centers for Disease Control and Prevention, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA
    Molecules 16:2391-413. 2011
  7. pmc Botulinum neurotoxin detection and differentiation by mass spectrometry
    John R Barr
    Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Emerg Infect Dis 11:1578-83. 2005
  8. ncbi request reprint Modifications to the organophosphorus nerve agent-protein adduct refluoridation method for retrospective analysis of nerve agent exposures
    Kerry E Holland
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3724, USA
    J Anal Toxicol 32:116-24. 2008
  9. ncbi request reprint Quantitation of the sulfur mustard metabolites 1,1'-sulfonylbis[2-(methylthio)ethane] and thiodiglycol in urine using isotope-dilution Gas chromatography-tandem mass spectrometry
    Anne E Boyer
    Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, Georgia 30341, USA
    J Anal Toxicol 28:327-32. 2004
  10. pmc Differentiation of Streptococcus pneumoniae conjunctivitis outbreak isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Yulanda M Williamson
    National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Appl Environ Microbiol 74:5891-7. 2008

Detail Information

Publications79

  1. doi request reprint A rapid method for capture and identification of immunogenic proteins in Bordetella pertussis enriched membranes fractions: a fast-track strategy applicable to other microorganisms
    Rolieria West
    Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Proteomics 75:1966-72. 2012
    ..IP-nLC-MS/MS provides a novel alternative approach for current methods used for the identification of immunogenic proteins...
  2. ncbi request reprint New high-resolution mass spectrometric approach for the measurement of polychlorinated biphenyls and organochlorine pesticides in human serum
    John R Barr
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Atlanta, GA 30341, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 794:137-48. 2003
    ..We used this method to analyze approximately 350 serum samples over a period of several months. We found that our new method was as reliable in automated, overnight runs as our current method...
  3. ncbi request reprint Analysis of urinary metabolites of sulfur mustard in two individuals after accidental exposure
    John R Barr
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
    J Anal Toxicol 32:10-6. 2008
    ..The bis-mercapturate of mustard sulfone was detected in patient 1 (3.1 ng/mL) on day 2 but was not detected in samples taken on subsequent days...
  4. ncbi request reprint Quantitation of metabolites of the nerve agents sarin, soman, cyclohexylsarin, VX, and Russian VX in human urine using isotope-dilution gas chromatography-tandem mass spectrometry
    John R Barr
    Centers for Disease Control and Prevention, National Center for Environmental Health, 4770 Buford Highway NE, Mailstop F 47, Atlanta, Georgia 30341, USA
    J Anal Toxicol 28:372-8. 2004
    ..Urine samples were acidified, extracted into ether-acetonitrile, derivatized by methylation with diazomethane, and analyzed by GC-MS-MS. The limits of detection were less than 1 micro g/L for all analytes...
  5. ncbi request reprint Measurement of toxaphene congeners in pooled human serum collected in three U.S. cities using high-resolution mass spectrometry
    John R Barr
    Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Arch Environ Contam Toxicol 46:551-6. 2004
    ..7-7 ng/g lipid) in pooled human serum collected in Atlanta, Chicago, and Cincinnati. We identified several analytical parameters that must be strengthened to routinely measure toxaphene congeners in human samples...
  6. doi request reprint Quantitative mass spectrometry for bacterial protein toxins--a sensitive, specific, high-throughput tool for detection and diagnosis
    Anne E Boyer
    Centers for Disease Control and Prevention, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA
    Molecules 16:2391-413. 2011
    ..We also describe potential applications to diseases of high public health impact, including Clostridium difficile glucosylating toxins and the Bordetella pertussis adenylyl cyclase...
  7. pmc Botulinum neurotoxin detection and differentiation by mass spectrometry
    John R Barr
    Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Emerg Infect Dis 11:1578-83. 2005
    ..62 MLD50/mL. A high-performance liquid chromatography-tandem mass spectrometry method for quantifying active toxin, where the amount of toxin can be correlated to the amount of product peptides, is also described...
  8. ncbi request reprint Modifications to the organophosphorus nerve agent-protein adduct refluoridation method for retrospective analysis of nerve agent exposures
    Kerry E Holland
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3724, USA
    J Anal Toxicol 32:116-24. 2008
    ..5 to 16.5 pg/mL for the G analogue of VX and GD, respectively. Characterization of quality control (QC) materials resulted in an average coefficient of variation of 15.1% for the five analytes in low QC pools and 11.7% in high QC pools...
  9. ncbi request reprint Quantitation of the sulfur mustard metabolites 1,1'-sulfonylbis[2-(methylthio)ethane] and thiodiglycol in urine using isotope-dilution Gas chromatography-tandem mass spectrometry
    Anne E Boyer
    Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, Georgia 30341, USA
    J Anal Toxicol 28:327-32. 2004
    ..The geometric mean concentration of TDG was 3.43 ng/mL, with concentrations ranging from < 0.5 ng/mL to 20 ng/mL...
  10. pmc Differentiation of Streptococcus pneumoniae conjunctivitis outbreak isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Yulanda M Williamson
    National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Appl Environ Microbiol 74:5891-7. 2008
    ....
  11. doi request reprint Subtyping botulinum neurotoxins by sequential multiple endoproteases in-gel digestion coupled with mass spectrometry
    Dongxia Wang
    National Center for Environmental Health, Centers for Disease Control and Prevention CDC, Atlanta, Georgia 30341, United States
    Anal Chem 84:4652-8. 2012
    ..Application of the method to a BoNT-contaminated carrot juice sample resulted in the identification of 98.4% protein sequence which led to a confident determination of the toxin subtype...
  12. doi request reprint A gel-free proteomic-based method for the characterization of Bordetella pertussis clinical isolates
    Yulanda M Williamson
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, Georgia 30341, USA
    J Microbiol Methods 90:119-33. 2012
    ..This novel technique is generally applicable allowing for the identification of immunogenic surface expressed proteins on pertussis and other pathogenic bacteria...
  13. ncbi request reprint A rapid, sensitive method for the quantitation of specific metabolites of sulfur mustard in human urine using isotope-dilution gas chromatography-tandem mass spectrometry
    Carrie L Young
    Centers for Disease Control and Prevention, National Center for Environmental Health, 4770 Buford Highway NE, Mailstop F 47, Atlanta, Georgia 30341, USA
    J Anal Toxicol 28:339-45. 2004
    ..Relative standard deviations were less than 8.6% at 1 ng/mL and 3.6% at 20 ng/mL. The limit of detection for SBMTE was 0.038 ng/mL in 0.5 mL of urine...
  14. pmc Extraction and inhibition of enzymatic activity of botulinum neurotoxins /B1, /B2, /B3, /B4, and /B5 by a panel of monoclonal anti-BoNT/B antibodies
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, N, E, Atlanta, GA 30341, USA
    BMC Biochem 12:58. 2011
    ..However, some antibodies inhibit or neutralize the enzymatic activity of BoNT, so the choice of antibody for toxin extraction is critical...
  15. doi request reprint Intermittent prophylaxis with oral truvada protects macaques from rectal SHIV infection
    J Gerardo Garcia-Lerma
    Division of HIV AIDS Prevention, National Center for HIV, Hepatitis, STD, and Prevention, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA
    Sci Transl Med 2:14ra4. 2010
    ..They strengthen the possibility of developing feasible, cost-effective strategies to prevent HIV transmission in humans...
  16. doi request reprint Improved detection of botulinum neurotoxin serotype A by Endopep-MS through peptide substrate modification
    Dongxia Wang
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Anal Biochem 432:115-23. 2013
    ..An optimized peptide increased assay sensitivity 5-fold with toxin spiked into buffer solution or different biological matrices...
  17. doi request reprint Comparison of MALDI-TOF-MS and HPLC-ESI-MS/MS for endopeptidase activity-based quantification of Anthrax lethal factor in serum
    Zsuzsanna Kuklenyik
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, United States
    Anal Chem 83:1760-5. 2011
    ..99 (r(2) = 0.997) and -0.25% mean relative difference (±9% standard deviation). This study shows that isotope dilution MALDI-TOF-MS is a robust and precise quantitative MS platform...
  18. pmc Extraction of BoNT/A, /B, /E, and /F with a single, high affinity monoclonal antibody for detection of botulinum neurotoxin by Endopep-MS
    Suzanne R Kalb
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America
    PLoS ONE 5:e12237. 2010
    ..When applied in conjunction with the Endopep-MS assay, the detection limit for each serotype of BoNT with this multi-specific monoclonal antibody is similar to that obtained when using other serotype-specific antibodies...
  19. ncbi request reprint Quantification of organophosphorus nerve agent metabolites using a reduced-volume, high-throughput sample processing format and liquid chromatography-tandem mass spectrometry
    Leigh L Swaim
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Emergency Response and Air Toxicants Branch, 4770 Buford Highway, MS F44, Atlanta, Georgia 30341, USA
    J Anal Toxicol 32:774-7. 2008
    ..In a public health emergency involving OP-nerve agents, this method provides the sample preparation and analytical capacity to respond rapidly to a large number of patient samples...
  20. ncbi request reprint Quantification of nerve agent VX-butyrylcholinesterase adduct biomarker from an accidental exposure
    Maria I Solano
    Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
    J Anal Toxicol 32:68-72. 2008
    ..05% of BuChE can be accurately quantified. These data further indicate that the fluoride ion regeneration method is a potentially powerful tool that can be used to assess low-level exposure to VX...
  21. ncbi request reprint From the mouse to the mass spectrometer: detection and differentiation of the endoproteinase activities of botulinum neurotoxins A-G by mass spectrometry
    Anne E Boyer
    National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Atlanta, Georgia 30341 3724, USA
    Anal Chem 77:3916-24. 2005
    ..039 to 0.625 mouse LD(50)/mL for toxin types A, B, E, and F in a buffer system. The Endopep-MS assay is the first to differentiate all seven BoNT types, is sensitive, specific, and has the potential to quantify toxin activity...
  22. pmc A historical and proteomic analysis of botulinum neurotoxin type/G
    Rebecca R Terilli
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA 30341, USA
    BMC Microbiol 11:232. 2011
    ..In addition, we review previous literature to provide a complete description of the BoNT/G complex...
  23. ncbi request reprint Quantification of L-abrine in human and rat urine: a biomarker for the toxin abrin
    Rudolph C Johnson
    Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F44, Atlanta, Georgia 30341, USA
    J Anal Toxicol 33:77-84. 2009
    ..Quantification of L-abrine beyond 24 h is limited by rapid excretion of the biomarker and the level of the L-abrine dose...
  24. ncbi request reprint Multianalyte quantification of five sesqui- and ethyl ether oxy-mustard metabolites in human urine by liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry
    Doris H Ash
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3724, USA
    J Anal Toxicol 32:44-50. 2008
    ....
  25. doi request reprint Studies on botulinum neurotoxins type /C1 and mosaic/DC using Endopep-MS and proteomics
    Hercules Moura
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA, USA
    FEMS Immunol Med Microbiol 61:288-300. 2011
    ..Additionally, we describe for the first time a proteomics study using a fast trypsin digestion method and label-free quantification of these toxin serotypes...
  26. doi request reprint Amino acid analysis of peptides using isobaric-tagged isotope dilution LC-MS/MS
    Adrian R Woolfitt
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
    Anal Chem 81:3979-85. 2009
    ..57% (range 2.72-4.20%). We applied this method to the quantification of three NIST standard peptides and hemagglutinin, an influenza virus surface protein...
  27. pmc Viable Staphylococcus aureus quantitation using ¹⁵N metabolically labeled bacteriophage amplification coupled with a multiple reaction monitoring proteomic workflow
    Carrie L Pierce
    National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F 50, Atlanta, GA 30341, USA
    Mol Cell Proteomics 11:M111.012849. 2012
    ..Amplification with (15)N bacteriophage coupled with LC-MS/MS detection offers speed (3 h total analysis time), sensitivity (LOD: < 5.0 × 10(4) CFU ml(-1)), accuracy, and precision for quantitation of S. aureus...
  28. pmc Different substrate recognition requirements for cleavage of synaptobrevin-2 by Clostridium baratii and Clostridium botulinum type F neurotoxins
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy N E, Atlanta, GA 30341, USA
    Appl Environ Microbiol 77:1301-8. 2011
    ..This peptide substrate is an N-terminal extension of the original peptide substrate used for detection of other BoNT/F toxins and can be used to detect four of the currently known BoNT/F subtypes by mass spectrometry...
  29. doi request reprint Analysis of active ricin and castor bean proteins in a ricin preparation, castor bean extract, and surface swabs from a public health investigation
    David M Schieltz
    Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Forensic Sci Int 209:70-9. 2011
    ..To the best of our knowledge following a review of the available literature, this report describes the most detailed analysis of a white powder for a public health or forensic investigation involving ricin...
  30. ncbi request reprint Standardization and denoising algorithms for mass spectra to classify whole-organism bacterial specimens
    Glen A Satten
    Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Bioinformatics 20:3128-36. 2004
    ..g. non-uniform baselines and higher noise associated with higher baseline), but are substantially noisier. As a result, new preprocessing procedures are required before these spectra can be used for statistical classification...
  31. ncbi request reprint Quantification of ricinine in rat and human urine: a biomarker for ricin exposure
    Rudolph C Johnson
    Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Mailstop F47, Atlanta, Georgia 3034, USA
    J Anal Toxicol 29:149-55. 2005
    ..These studies show that ricinine can be measured in rat urine at least 48 h after exposure. Ricinine is present in crude preparations of ricin, and it can be found in human urine after a lethal exposure to ricin...
  32. ncbi request reprint Quantitation of biomarkers of exposure to nitrogen mustards in urine from rats dosed with nitrogen mustards and from an unexposed human population
    Sharon W Lemire
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3724, USA
    J Anal Toxicol 28:320-6. 2004
    ..Neither EDEA (LOD 0.4 ng/mL) nor MDEA (LOD 0.8 ng/mL) was detected above the LOD in the human samples...
  33. doi request reprint Quantification of immunoreactive viral influenza proteins by immunoaffinity capture and isotope-dilution liquid chromatography-tandem mass spectrometry
    Carrie L Pierce
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F 50, Atlanta, Georgia 30341, USA
    Anal Chem 83:4729-37. 2011
    ..IC-IDMS is an accurate, precise, sensitive, and selective method to measure antibody-bound HA in purified virus and commercial vaccines...
  34. doi request reprint Simultaneous quantification of hemagglutinin and neuraminidase of influenza virus using isotope dilution mass spectrometry
    Tracie L Williams
    Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, United States
    Vaccine 30:2475-82. 2012
    ....
  35. ncbi request reprint Survey of albumin purification methods for the analysis of albumin-organic toxicant adducts by liquid chromatography-electrospray ionization-tandem mass spectrometry
    Carrie L Young
    National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway NE, MS F47 Atlanta, GA 30341, USA
    Proteomics 5:4973-9. 2005
    ....
  36. ncbi request reprint The use of Endopep-MS for the detection of botulinum toxins A, B, E, and F in serum and stool samples
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health Agency for Toxic Substances and Disease Registry, Atlanta, GA 30341, USA
    Anal Biochem 351:84-92. 2006
    ..The limits of detection in spiked stool extracts are somewhat higher due to the high-protease environment of stool extract that also requires use of protease inhibitors. The entire method can be performed in as short a time as 4 h...
  37. pmc Lethal factor toxemia and anti-protective antigen antibody activity in naturally acquired cutaneous anthrax
    Anne E Boyer
    Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    J Infect Dis 204:1321-7. 2011
    ..This is the first report of quantitative analysis of serum LF in cutaneous anthrax and the first to associate acute stage toxemia with subsequent antitoxin antibody responses...
  38. pmc Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype
    Brian H Raphael
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    BMC Microbiol 12:245. 2012
    ..Previous studies showed that the amino acid sequences of BoNT/E produced by various strains differ by < 6% and that the type E neurotoxin gene cluster inserts into the rarA operon...
  39. doi request reprint Recovery and detection of botulinum neurotoxins from a nonporous surface
    Matthew Lautenschlager
    Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    J Microbiol Methods 92:278-80. 2013
    ..In addition, we demonstrate that this method can be used to evaluate the efficacy of surface decontamination procedures...
  40. doi request reprint Detection and quantification of ricin in beverages using isotope dilution tandem mass spectrometry
    Sara C McGrath
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
    Anal Chem 83:2897-905. 2011
    ..This method was used successfully to extract ricin from each beverage matrix. The activity of recovered ricin was assessed, and quantification was achieved, with a limit of detection of 10 fmol/mL (0.64 ng/mL)...
  41. pmc De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA 30341, USA
    Anal Bioanal Chem 403:215-26. 2012
    ..These techniques allow for subtype and strain level identification of both known and unknown BoNT/B rapidly with no DNA required...
  42. doi request reprint Quantification of botulinum neurotoxin serotypes A and B from serum using mass spectrometry
    Bryan A Parks
    Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia, USA
    Anal Chem 83:9047-53. 2011
    ..This is the first report of a quantitative method with sufficient sensitivity, selectivity, and low sample size requirements to measure circulating BoNT activity at multiple times during the course of botulism...
  43. pmc Discovery of a novel enzymatic cleavage site for botulinum neurotoxin F5
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA
    FEBS Lett 586:109-15. 2012
    ..Here, we report that BoNT/F5 cleaves substrate synaptobrevin-2 in a different location than the other BoNT/F subtypes, between (54)L and (55)E. This is the first report of cleavage of synaptobrevin-2 in this location...
  44. pmc Antibody responses to a spore carbohydrate antigen as a marker of nonfatal inhalation anthrax in rhesus macaques
    Elke Saile
    Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Division of Bacterial Diseases, Atlanta, Georgia 30333, USA
    Clin Vaccine Immunol 18:743-8. 2011
    ..These data suggest that anti-ATS antibody responses may be used to identify aerosol exposure to B. anthracis spores. The anti-ATS antibody responses were detectable during administration of ciprofloxacin...
  45. doi request reprint On-line coupling of anion exchange and ion-pair chromatography for measurement of intracellular triphosphate metabolites of reverse transcriptase inhibitors
    Zsuzsanna Kuklenyik
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, United States
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3659-66. 2009
    ..Limits of detection were 9, 200 and 75 pg per sample for TFV-DP (448/176 m/z), FTC-TP (488/130 m/z) and 3TC-TP (468/119 m/z), respectively...
  46. doi request reprint Targeted N-linked glycosylation analysis of H5N1 influenza hemagglutinin by selective sample preparation and liquid chromatography/tandem mass spectrometry
    Thomas A Blake
    Biological Mass Spectrometry Laboratory, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F 50, Atlanta, Georgia 30341, USA
    Anal Chem 81:3109-18. 2009
    ....
  47. doi request reprint Optimization of digestion parameters for protein quantification
    Jessica Norrgran
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F 50, Atlanta, GA 30341, USA
    Anal Biochem 393:48-55. 2009
    ..The analysis was performed by liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode using isotopically labeled peptide standards for quantification...
  48. ncbi request reprint Comprehensive solid-phase extraction method for persistent organic pollutants. Validation and application to the analysis of persistent chlorinated pesticides
    Courtney D Sandau
    National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway NE, Mailstop F17, Atlanta, Georgia, 30341 3724, USA
    Anal Chem 75:71-7. 2003
    ..The method represents a preliminary step in establishing an automated, comprehensive multiresidue analysis method for POPs in human serum...
  49. pmc Measurement of p-nitrophenol in the urine of residents whose homes were contaminated with methyl parathion
    Dana B Barr
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Environ Health Perspect 110:1085-91. 2002
    ....
  50. ncbi request reprint Detection of botulinum neurotoxin A in a spiked milk sample with subtype identification through toxin proteomics
    Suzanne R Kalb
    National Center for Environmental Health Agency for Toxic Substances and Disease Registry, Centers for Disease Control and Prevention, 4770 Buford Highway, NE Atlanta, Georgia 30341 3724, USA
    Anal Chem 77:6140-6. 2005
    ..In its entirety, this method would allow for analysis of BoNT with toxin type identification in a few hours and subtype identification within 24 h...
  51. pmc Extraction and inhibition of enzymatic activity of botulinum neurotoxins/A1, /A2, and /A3 by a panel of monoclonal anti-BoNT/A antibodies
    Suzanne R Kalb
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA, USA
    PLoS ONE 4:e5355. 2009
    ..The mAbs binding the C-terminal portion of the BoNT/A heavy chain had optimal properties for use in the Endopep-MS assay...
  52. doi request reprint Mass spectrometric detection of ricin and its activity in food and clinical samples
    Suzanne R Kalb
    Centers for Disease Control and Prevention, NCEH DLS, 4770 Buford Highway, NE Atlanta, Georgia 30341 3724, USA
    Anal Chem 81:2037-42. 2009
    ..This measurement includes a measure of the toxin's activity. The utility of this method was demonstrated on ricin spiked into food and clinical samples consisting of milk, apple juice, serum, and saliva...
  53. ncbi request reprint Detection and quantification of anthrax lethal factor in serum by mass spectrometry
    Anne E Boyer
    Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Highway, NE, Atlanta, Georgia 30341, USA
    Anal Chem 79:8463-70. 2007
    ..This method represents a highly specific and rapid diagnostic tool for early anthrax and has a potential additional role as a research tool for understanding toxemia and effects of medical countermeasures for anthrax...
  54. ncbi request reprint Identification and quantification of diethylene glycol in pharmaceuticals implicated in poisoning epidemics: an historical laboratory perspective
    Dana B Barr
    National Center of Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    J Anal Toxicol 31:295-303. 2007
    ..This methodology includes the development of biomarkers of DEG exposure, which would be extremely useful in instances where pharmaceuticals are not clearly implicated...
  55. ncbi request reprint Mass spectral behavior of the hydrolysis products of sesqui- and oxy-mustard type chemical warfare agents in atmospheric pressure chemical ionization
    Sharon W Lemire
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3724, USA
    J Am Soc Mass Spectrom 18:1364-74. 2007
    ..Thus, we are the first to propose a quantitative method predicated on the measurement of product ions generated from the superoxide adduct anion of the sesqui-and oxy-mustard hydrolysis products...
  56. pmc MALDI-TOF mass spectrometry as a tool for differentiation of invasive and noninvasive Streptococcus pyogenes isolates
    Hercules Moura
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, GA 30341, USA
    FEMS Immunol Med Microbiol 53:333-42. 2008
    ..Almost 30% of the biomarkers detected were tentatively identified as ribosomal proteins...
  57. ncbi request reprint Identification of biomarkers of whole Coxiella burnetii phase I by MALDI-TOF mass spectrometry
    Edward I Shaw
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    Anal Chem 76:4017-22. 2004
    ..burnetii isolates, especially when coupled with antigen capture techniques. The method also has potential for several applications in basic microbial research, including regulation of gene expression...
  58. pmc Kinetics of lethal factor and poly-D-glutamic acid antigenemia during inhalation anthrax in rhesus macaques
    Anne E Boyer
    Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Infect Immun 77:3432-41. 2009
    ..This study emphasizes the value of LF detection as a tool for early diagnosis of inhalation anthrax before the onset of fulminant systemic infection...
  59. pmc Mass spectrometric analysis of multiple pertussis toxins and toxoids
    Yulanda M Williamson
    Biological Mass Spectrometry Laboratory, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA 30341, USA
    J Biomed Biotechnol 2010:942365. 2010
    ..Additionally, based on a label-free mass spectrometry-based quantification method there is differential relative abundance of the subunits among the sources...
  60. doi request reprint Detection of Staphylococcus aureus using 15N-labeled bacteriophage amplification coupled with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry
    Carrie L Pierce
    National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F 50, Atlanta, Georgia 30341, United States
    Anal Chem 83:2286-93. 2011
    ..This method overcomes current limitations by improving analysis times while increasing selectivity when compared to previously reported PAD methodologies...
  61. doi request reprint Ultra performance liquid chromatography isotope dilution tandem mass spectrometry for the absolute quantification of proteins and peptides
    Leah G Luna
    Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F 50, Atlanta, Georgia 30341, USA
    Anal Chem 80:2688-93. 2008
    ..UPLC provides additional dimensions of rapid analysis time and high-sample throughput, which expands laboratory emergency response capabilities over conventional HPLC...
  62. doi request reprint Improved detection of botulinum neurotoxin type A in stool by mass spectrometry
    Dongxia Wang
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Anal Biochem 412:67-73. 2011
    ..Here we report a method to reduce nonspecific substrate cleavage by reducing stool protease coextraction in the Endopep-MS assay...
  63. ncbi request reprint Analysis of four human microsporidian isolates by MALDI-TOF mass spectrometry
    Hercules Moura
    Atlanta Research and Education Foundation, Rio de Janeiro, Brazil
    J Eukaryot Microbiol 50:156-63. 2003
    ..MALDI-TOF MS analysis of whole organisms is a rapid, sensitive, and specific option to characterize microsporidian isolates and has the potential for several applications in parasitology...
  64. doi request reprint Quantification of influenza virus hemagglutinins in complex mixtures using isotope dilution tandem mass spectrometry
    Tracie L Williams
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Vaccine 26:2510-20. 2008
    ....
  65. ncbi request reprint Effect of mobile phase pH and organic content on LC-MS analysis of nucleoside and nucleotide HIV reverse transcriptase inhibitors
    Zsuzsanna Kuklenyik
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
    J Chromatogr Sci 47:365-72. 2009
    ..1 mL plasma (10 pg on column) and coefficients of variation from 5% to 15% in the high ng/mL concentration range and from 16% to 20% in the low ng/mL concentration range...
  66. ncbi request reprint A multi-analyte method for the quantification of contemporary pesticides in human serum and plasma using high-resolution mass spectrometry
    Dana B Barr
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Hwy NE, Mailstop F17, Atlanta, GA 30341, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 778:99-111. 2002
    ..We found detectable concentrations of carbaryl/naphthalene, propoxur, bendiocarb, chlorpyrifos, diazinon, dicloran, captan and folpet or their metabolites in more than 20% of the plasma samples tested...
  67. ncbi request reprint Analysis of recombinant acylated pneumococcal surface adhesin A of Streptococcus pneumoniae by mass spectrometry
    Barun K De
    Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Arch Biochem Biophys 419:147-57. 2003
    ..The availability of well-characterized rPsaA will facilitate the continued research and development of protein-based vaccines for the prevention of pneumococcal disease...
  68. doi request reprint Design of online solid phase extraction-liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) hyphenated systems for quantitative analysis of small organic compounds in biological matrices
    Zsuzsanna Kuklenyik
    Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    J Sep Sci 34:3606-18. 2011
    ....
  69. ncbi request reprint Quantification of saxitoxin and neosaxitoxin in human urine utilizing isotope dilution tandem mass spectrometry
    Rudolph C Johnson
    Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, MS F44, Atlanta, Georgia 30341, USA
    J Anal Toxicol 33:8-14. 2009
    ..These two toxins were not detected in a reference range of humans who consumed seafood in the preceding 72 h, suggesting that few false positives would occur when trying to identify people exposed to STX or NEO...
  70. ncbi request reprint Analysis of biological samples for chemical warfare agents
    John R Barr
    J Anal Toxicol 32:1. 2008
  71. ncbi request reprint Analysis for plasma protein biomarkers following an accidental human exposure to sulfur mustard
    J Richard Smith
    US Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Road, Aberdeen Proving Ground, Maryland 21010 5400, USA
    J Anal Toxicol 32:17-24. 2008
    ..The data presented here add significantly to the small database of information that currently exists on human biomarkers of sulfur mustard exposure, linking a well-documented exposure event with levels of plasma protein adducts...
  72. pmc Personalized exposure assessment: promising approaches for human environmental health research
    Brenda K Weis
    Division of Extramural Research and Training, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Environ Health Perspect 113:840-8. 2005
    ..Improved methods for exposure assessment will result in better means of monitoring and targeting intervention and prevention programs...
  73. ncbi request reprint IFCC reference system for measurement of hemoglobin A1c in human blood and the national standardization schemes in the United States, Japan, and Sweden: a method-comparison study
    Wieland Hoelzel
    Roche Diagnostics GmbH, Penzberg, Germany
    Clin Chem 50:166-74. 2004
    ..The aim of the present study was to determine the relationships between the IFCC Reference Method (RM) and the DCMs...
  74. ncbi request reprint Improvements of the fluoride reactivation method for the verification of nerve agent exposure
    Carla E A M Degenhardt
    TNO Prins Maurits Laboratory, P O Box 45, 2280 AA Rijswijk, The Netherlands
    J Anal Toxicol 28:364-71. 2004
    ..Detection limits of approximately 10 pg/mL plasma were achieved for all analytes, which corresponds to 0.09% inhibition with GB on a sample with normal BuChE levels...
  75. ncbi request reprint Biological monitoring of human exposure to chemical warfare agents
    John R Barr
    J Anal Toxicol 28:305. 2004
  76. ncbi request reprint Strain and phase identification of the U.S. category B agent Coxiella burnetii by matrix assisted laser desorption/ionization time-of-flight mass spectrometry and multivariate pattern recognition
    Carrie Y Pierce
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, United States
    Anal Chim Acta 583:23-31. 2007
    ..The method was validated by the prediction of unknown C. burnetii samples in an independent test set with 100% sensitivity and specificity for five out of six strain classes...
  77. pmc Contemporary-use pesticides in personal air samples during pregnancy and blood samples at delivery among urban minority mothers and newborns
    Robin M Whyatt
    Columbia Center for Children s Environmental Health, Mailman School of Public Health, Columbia University, New York, New York, USA
    Environ Health Perspect 111:749-56. 2003
    ..Findings indicate that pesticide exposures are frequent but decreasing and that the pesticides are readily transferred to the developing fetus during pregnancy...
  78. ncbi request reprint A case of naturally acquired inhalation anthrax: clinical care and analyses of anti-protective antigen immunoglobulin G and lethal factor
    James J Walsh
    Section of Pulmonary and Critical Care Medicine, Guthrie Clinic, Sayre, PA, USA
    Clin Infect Dis 44:968-71. 2007
    ..Clinical correlation of serologic assays for the lethal factor component of lethal toxin and anti-protective antigen immunoglobulin G are also presented...
  79. ncbi request reprint Detection of botulinum type E toxin in channel catfish with visceral toxicosis syndrome using catfish bioassay and endopep mass spectrometry
    Patricia S Gaunt
    Mississippi State University College of Veterinary Medicine, Stoneville, MS 38776, USA
    J Vet Diagn Invest 19:349-54. 2007
    ..Although there was not enough botulinum present in affected samples to obtain exact quantification, the estimated quantity of botulinum E in these sera samples was between 0.01 and 0.5 mouse LD50/ml...