Research Topics
| David L AshleySummaryAffiliation: Centers for Disease Control and Prevention Country: USA Publications
| Collaborators
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Detail Information
Publications
Determination of carcinogenic tobacco-specific nitrosamines in mainstream smoke from U.S.-brand and non-U.S.-brand cigarettes from 14 countriesWeijia Wu
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
Nicotine Tob Res 7:443-51. 2005....- Quantification of 31 volatile organic compounds in whole blood using solid-phase microextraction and gas chromatography-mass spectrometryBenjamin C Blount
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
J Chromatogr B Analyt Technol Biomed Life Sci 832:292-301. 2006..12 microg/L, with linear calibration curves spanning three orders of magnitude. The improved throughput of this method will enable us to expand biomonitoring efforts to assess nonoccupational VOC exposure in large epidemiological studies... 
Automated determination of seven phenolic compounds in mainstream tobacco smokeChristina Vaughan
Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laborarory Sciences, Emergency Response and Air Toxicants Branch, Atlanta, GA 31314, USA
Nicotine Tob Res 10:1261-8. 2008..Differences were seen in relative levels of phenolic compounds in the mainstream smoke from unfiltered cigarettes made with a single type of tobacco...- Effect of differing levels of tobacco-specific nitrosamines in cigarette smoke on the levels of biomarkers in smokersDavid L Ashley
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Cancer Epidemiol Biomarkers Prev 19:1389-98. 2010..Previous studies have shown significant global differences in the levels of TSNAs in cigarette smoke because of the variation in tobacco blending and curing practices around the world... - Changes in blood trihalomethane concentrations resulting from differences in water quality and water use activitiesDavid L Ashley
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
Arch Environ Occup Health 60:7-15. 2005..Further studies will focus on variables in these activities that can alter exposure... - Tobacco-specific nitrosamines in tobacco from U.S. brand and non-U.S. brand cigarettesDavid L Ashley
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA, USA
Nicotine Tob Res 5:323-31. 2003..S. brands purchased in the same country. The findings suggest that TSNA levels in tobacco can be substantially reduced in some cigarettes... - Semi-volatiles in mainstream smoke delivery from select charcoal-filtered cigarette brand variantsBryan A Hearn
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE MS F 47, Atlanta, GA 30341, USA
Tob Control 19:223-30. 2010.... - Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in smokers in the United States: NHANES 2007-2008Yang Xia
Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Biomarkers 16:112-9. 2011..001). In addition to addressing current NNK exposure levels, these results will form a baseline for future estimates of tobacco users' exposure to this carcinogen... - Levels of tobacco-specific nitrosamines and polycyclic aromatic hydrocarbons in mainstream smoke from different tobacco varietiesYan S Ding
Emergency Response and Air Toxicants Branch, Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway Northeast, Mailstop F 47, Atlanta, GA 30341 3724, USA
Cancer Epidemiol Biomarkers Prev 17:3366-71. 2008..The influence of nitrate on mainstream smoke NNK and PAH levels were of different magnitude and direction. Our results tend to indicate an inverse relation exists between NNK and PAH deliveries when considering different tobacco blends... - Urine concentrations of a tobacco-specific nitrosamine carcinogen in the U.S. population from secondhand smoke exposureJohn T Bernert
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Cancer Epidemiol Biomarkers Prev 19:2969-77. 2010..We have measured total NNAL in the U.S. population of tobacco users and nonsmokers exposed to secondhand smoke... 
Botulinum neurotoxin detection and differentiation by mass spectrometryJohn R Barr
Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Emerg Infect Dis 11:1578-83. 2005..62 MLD50/mL. A high-performance liquid chromatography-tandem mass spectrometry method for quantifying active toxin, where the amount of toxin can be correlated to the amount of product peptides, is also described...- Estimating smokers' mouth-level exposure to select mainstream smoke constituents from discarded cigarette filter buttsGregory M Polzin
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, N E, Mailstop F 55, Atlanta, GA 30341, USA
Nicotine Tob Res 11:868-74. 2009.... - Determination of eugenol, anethole, and coumarin in the mainstream cigarette smoke of Indonesian clove cigarettesGregory M Polzin
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, N E Atlanta, GA 30341, USA
Food Chem Toxicol 45:1948-53. 2007..8-1,030 microg/cig, and coumarin was detected in 19 brands at levels ranging from 9.2 to 215 microg/cig. These detected levels are significantly higher than the levels found in commercial cigarette brands available in the United States... - Quantification of nerve agent VX-butyrylcholinesterase adduct biomarker from an accidental exposureMaria I Solano
Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
J Anal Toxicol 32:68-72. 2008..05% of BuChE can be accurately quantified. These data further indicate that the fluoride ion regeneration method is a potentially powerful tool that can be used to assess low-level exposure to VX... - Increases in tobacco exposure biomarkers measured in non-smokers exposed to sidestream cigarette smoke under controlled conditionsJohn T Bernert
Division of Laboratory Science, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Biomarkers 14:82-93. 2009.... - Determination of 10 carcinogenic polycyclic aromatic hydrocarbons in mainstream cigarette smokeYan S Ding
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mailstop F 47, Atlanta, Georgia 30341 3724, USA
J Agric Food Chem 55:5966-73. 2007..However, if highly ventilated cigarettes were smoked under more intense conditions (e.g., larger or more frequent puffs, vents blocked), their PAH levels equaled or exceeded their full-flavor counterparts under ISO conditions... - Determination of 14 polycyclic aromatic hydrocarbons in mainstream smoke from U.S. brand and non-U.S. brand cigarettesYan S Ding
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway NE, Mailstop F-47, Atlanta, Georgia 30341, USA
Environ Sci Technol 40:1133-8. 2006..The findings suggest that tobacco blend and nitrate levels may influence PAH deliveries, but other factors may confound this relation... - Determination of tar, nicotine, and carbon monoxide yields in the smoke of bidi cigarettesClifford H Watson
Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, GA 30341, USA
Nicotine Tob Res 5:747-53. 2003..7+/-.4 mg/bidi), and CO (39.2+/-5.7 mg/bidi). In comparison, traditional cigarettes smoked using the bidi cigarette protocol have lower tar and CO yields, but have nicotine deliveries comparable with bidi cigarettes... - Analysis of urinary metabolites of sulfur mustard in two individuals after accidental exposureJohn R Barr
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
J Anal Toxicol 32:10-6. 2008..The bis-mercapturate of mustard sulfone was detected in patient 1 (3.1 ng/mL) on day 2 but was not detected in samples taken on subsequent days... - Global surveillance of oral tobacco products: total nicotine, unionised nicotine and tobacco-specific N-nitrosaminesStephen B Stanfill
Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Emergency Response and Air Toxicants Branch, 4770 Buford Highway, Atlanta, Georgia 30341, USA
Tob Control 20:e2. 2011..The aim of this study was to determine typical pH ranges and concentrations of total nicotine, unionised nicotine (the most readily absorbed form) and five TSNAs in selected oral tobacco products distributed globally... - Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS)K Udeni Alwis
National Center for Environmental Health, Division of Laboratory Sciences, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
Anal Chim Acta 750:152-60. 2012..0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics... - Quantitative analysis of six heterocyclic aromatic amines in mainstream cigarette smoke condensate using isotope dilution liquid chromatography-electrospray ionization tandem mass spectrometryLiqin Zhang
Emergency Response and Air Toxicants Branch, Division of Laboratory Science, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mailstop F 47, Atlanta, GA 30341 3724, USA
Nicotine Tob Res 13:120-6. 2011..We have developed and validated a straightforward high-throughput method to quantify HAA levels in mainstream cigarette smoke and demonstrated the applicability by analyzing select research and domestic cigarette brands... - Simultaneous determination of five tobacco-specific nitrosamines in mainstream cigarette smoke by isotope dilution liquid chromatography/electrospray ionization tandem mass spectrometryWeijia Wu
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, Georgia 30341, USA
Anal Chem 75:4827-32. 2003..This new method provides higher sensitivity, specificity, and throughput than earlier methods for TSNA determination... - Quantification of fuel oxygenate ethers in human blood using solid-phase microextraction coupled with gas chromatography-high-resolution mass spectrometryLalith K Silva
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
J Anal Toxicol 32:273-80. 2008..7 to 5.4%) and accuracy (96-100%). This method provides a means to assess fuel oxygenate exposure and study the potential relationship between exposure and adverse health outcomes... - Assessment of tobacco-specific nitrosamines in the tobacco and mainstream smoke of Bidi cigarettesWeijia Wu
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, Mailstop F-47, Atlanta, GA 30341, USA
Carcinogenesis 25:283-7. 2004..Our analytical findings concur with the previous biologic and biochemical evidence supporting epidemiologic studies linking bidi use with various cancers, especially oral cavity and lung cancers... - Quantitation of biomarkers of exposure to nitrogen mustards in urine from rats dosed with nitrogen mustards and from an unexposed human populationSharon W Lemire
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3724, USA
J Anal Toxicol 28:320-6. 2004..Neither EDEA (LOD 0.4 ng/mL) nor MDEA (LOD 0.8 ng/mL) was detected above the LOD in the human samples... - Simultaneous determination of six mercapturic acid metabolites of volatile organic compounds in human urineYan S Ding
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mailstop F 47, Atlanta, Georgia 30341 3724, USA
Chem Res Toxicol 22:1018-25. 2009..4, 839.7, 10.2, 509.7, 2.2, and 0.9 microg/g creatinine, respectively. All VOC MAs measured were higher among smokers than among nonsmokers, with the exception of MHBMA... - Analysis of volatile organic compounds in mainstream cigarette smokeGregory M Polzin
Emergency Response and Air Toxicants Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, USA
Environ Sci Technol 41:1297-302. 2007..In this report we demonstrate the method's analytical capabilities by quantitatively analyzing 13 selected VOCs in mainstream cigarette smoke from top-selling domestic brands... - Solid-phase microextraction-based approach to determine free-base nicotine in trapped mainstream cigarette smoke total particulate matterClifford H Watson
Emergency Response and Air Toxicants Branch, Mailstop F 47, Centers for Disease Control and Prevention, 4770 Buford Highway N E, Atlanta, GA 30341 3724, USA
J Agric Food Chem 52:7240-5. 2004..01 to 0.08 mg/cigarette. The measured ranges of free-base nicotine were remarkably similar over the different tar and nicotine delivery categories of full-flavored, light, and ultralight cigarette brands... - Measurement of trihalomethanes and methyl tert-butyl ether in whole blood using gas chromatography with high-resolution mass spectrometryMichael A Bonin
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
J Anal Toxicol 29:81-9. 2005..3 to 2.4 ng/L, with linear ranges of three orders of magnitude. This method proved adequate for measuring the THMs and MTBE in most blood samples tested from a diverse U.S. reference population... - The influence of physicochemical properties on the internal dose of trihalomethanes in humans following a controlled showering exposureLalith K Silva
Division of Laboratory Sciences, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
J Expo Sci Environ Epidemiol 23:39-45. 2013..The physicochemical properties (volatility, partition coefficients, and Henry's law constant) are useful parameters for predicting THM movement between matrices and understanding THM exposure during showering... - Measurement of trihalomethanes and methyl tertiary-butyl ether in tap water using solid-phase microextraction GC-MSFrederick L Cardinali
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
J Chromatogr Sci 42:200-6. 2004..THMs are stable for extended periods in tap water samples after quenching of residual chlorine and buffering to pH 6.5, thus enabling larger epidemiologic field studies with simplified sample collection protocols... - Quantification of flavor-related compounds in the unburned contents of bidi and clove cigarettesStephen B Stanfill
Division of Laboratory Sciences, Emergency Response and Air Toxicants Branch, National Center for Environmental Health, U S Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3719, USA
J Agric Food Chem 54:8580-8. 2006..S. cigarette tobacco. Research is underway to determine the levels of these compounds delivered in smoke. It is not known what effect inhalation of these compounds has on smokers... - Biological exposure assessment to tetrachloroethylene for workers in the dry cleaning industryLauralynn T McKernan
Centers for Disease Control and Prevention CDC National Institute for Occupational Safety and Health, 4676 Columbia Parkway, R 15, Cincinnati, OH 45226, USA
Environ Health 7:12. 2008..The purpose of this study was to assess the feasibility of conducting biological tetrachloroethylene (perchloroethylene, PCE) exposure assessments of dry cleaning employees in conjunction with evaluation of possible PCE health effects... - Effect of perchloroethylene, smoking, and race on oxidative DNA damage in female dry cleanersMark Toraason
The National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
Mutat Res 539:9-18. 2003..Results indicate a reduction in oxidative DNA damage in PERC exposed dry cleaners relative to launderers, but PERC could not clearly be defined as the source of the effect... - Determination of nicotine and other minor alkaloids in international cigarettes by solid-phase microextraction and gas chromatography/mass spectrometryWeijia Wu
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Anal Chem 74:4878-84. 2002..These results strongly suggest that such differences reflect variations associated with blend compositions, tobacco quality, and manufacturing practices... - Urinary levels of trichloroacetic acid, a disinfection by-product in chlorinated drinking water, in a human reference populationAntonia M Calafat
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Environ Health Perspect 111:151-4. 2003.... - Quantitative determination of the hydrolysis products of nitrogen mustards in human urine by liquid chromatography-electrospray ionization tandem mass spectrometrySharon W Lemire
Division of Laboratory Sciences, National Center for Environmental Health Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3724, USA
J Anal Toxicol 27:1-6. 2003..The method limits of detection were 0.41 ng/mL for EDEA and 0.96 ng/mL for MDEA in 1 mL of urine with coefficients of variation < 10% for both compounds... - From the mouse to the mass spectrometer: detection and differentiation of the endoproteinase activities of botulinum neurotoxins A-G by mass spectrometryAnne E Boyer
National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Atlanta, Georgia 30341 3724, USA
Anal Chem 77:3916-24. 2005..039 to 0.625 mouse LD(50)/mL for toxin types A, B, E, and F in a buffer system. The Endopep-MS assay is the first to differentiate all seven BoNT types, is sensitive, specific, and has the potential to quantify toxin activity... - Quantitative detection of trichloroacetic acid in human urine using isotope dilution high-performance liquid chromatography-electrospray ionization tandem mass spectrometryZsuzsanna Kuklenyik
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Anal Chem 74:2058-63. 2002..The method is simple and fast and is not labor intensive (sample preparation and analysis can be performed in approximately 15 min) with a limit of detection of 0.5 ng/mL in 1 mL of urine... - Quantitation of 2-chlorovinylarsonous acid in human urine by automated solid-phase microextraction--gas chromatography--mass spectrometryJoe V Wooten
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, NE, Mailstop F19, Atlanta, GA 30341, USA
J Chromatogr B Analyt Technol Biomed Life Sci 772:147-53. 2002..The assay was validated on human urine samples spiked with CVAA to determine the accuracy, precision, and limit of detection (LOD). The LOD was 7.4 pg in 1 ml of urine... - The development and testing of a dermal exposure system for pharmacokinetic studies of administered and ambient water contaminantsJames D Prah
National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, MD 58B, Research Triangle Park, NC 27711, USA
J Pharmacol Toxicol Methods 47:189-95. 2002..In order to investigate the pharmacokinetics of water-borne chemicals while eliminating exposures by other routes, a dermal exposure system was developed to expose the hand and forearm of human subjects... - Assessing exposure in epidemiologic studies to disinfection by-products in drinking water: report from an international workshopTye E Arbuckle
Bureau of Reproductive and Child Health, Health Canada, Ottawa, Ontario, Canada
Environ Health Perspect 110:53-60. 2002..Similarly, exposure classification categories in epidemiologic studies should be chosen to make results useful for regulatory or policy decision making... - Changes in breath trihalomethane levels resulting from household water-use activitiesSydney M Gordon
Battelle Memorial Institute, 505 King Avenue, Columbus, OH 43201, USA
Environ Health Perspect 114:514-21. 2006..Future studies will address the effects that changes in these common water-use activities may have on exposure... - The recommended role of exposure biomarkers for the surveillance of environmental and occupational chemical exposures in military deployments: policy considerationsLisa M May
Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Room A1044, Bethesda, MD 20814-4799, USA
Mil Med 169:761-7. 2004.... - Children's exposure to volatile organic compounds as determined by longitudinal measurements in bloodKen Sexton
University of Texas School of Public Health, Brownsville Regional Campus, Brownsville, Texas, USA
Environ Health Perspect 113:342-9. 2005..For siblings living in the same household, we found strong statistical associations between measured blood VOC concentrations... - Influence of tap water quality and household water use activities on indoor air and internal dose levels of trihalomethanesJohn R Nuckols
Environmental Health Advanced Systems Laboratory, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado 80523 1681, USA
Environ Health Perspect 113:863-70. 2005..Further research is also needed in designing epidemiologic studies that minimize data collection burden yet maximize accuracy in classification of dermal and inhalation THM exposure during hot water use activities... - Using biologic markers in blood to assess exposure to multiple environmental chemicals for inner-city children 3-6 years of ageKen Sexton
University of Texas School of Public Health, Brownsville, Texas 78520 4956, USA
Environ Health Perspect 114:453-9. 2006..Results demonstrate that cumulative exposures to multiple environmental carcinogens and neurotoxins can be comparatively high for children from a poor inner-city neighborhood... - Comparison of trihalomethanes in tap water and bloodAmy M Miles
Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599-7400, USA
Environ Sci Technol 36:1692-8. 2002..This finding suggests that other factors, in addition to tap water concentrations, may be important in determining THM concentrations in the blood...