Roberta A Gottlieb
Affiliation: Cedars-Sinai Medical Center
Stastna M, Thomas A, Germano J, Pourpirali S, Van Eyk J, Gottlieb R. Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion. J Vis Exp. 2018;: pubmed publisher
..Here we present the methods used to accomplish this analysis-in particular, the approach to optimization of protein extraction from the sucrose gradient, as this has not been described before-and provide some representative results. ..
Andres A, Stotland A, Queliconi B, Gottlieb R. A time to reap, a time to sow: mitophagy and biogenesis in cardiac pathophysiology. J Mol Cell Cardiol. 2015;78:62-72 pubmed publisher
..This article is part of a Special Issue entitled "Mitochondria: From Basic Mitochondrial Biology to Cardiovascular Disease". ..
Gottlieb R, Stotland A. MitoTimer: a novel protein for monitoring mitochondrial turnover in the heart. J Mol Med (Berl). 2015;93:271-8 pubmed publisher
..Mitochondrial biogenesis must keep pace with mitophagy in order to maintain a stable number of mitochondria. In this review, we will discuss the use of MitoTimer as a tool to monitor mitochondrial turnover. ..
Linton P, Gurney M, Sengstock D, Mentzer R, Gottlieb R. This old heart: Cardiac aging and autophagy. J Mol Cell Cardiol. 2015;83:44-54 pubmed publisher
..This article is part of a Special Issue entitled CV Aging. ..
Stotland A, Gottlieb R. Mitochondrial quality control: Easy come, easy go. Biochim Biophys Acta. 2015;1853:2802-11 pubmed publisher
..The review also summarizes the current methods and techniques for analyzing mitochondrial turnover in vivo and in vitro, from the whole animal proteome level to the level of single organelle. ..
Gottlieb R, Andres A, Sin J, Taylor D. Untangling autophagy measurements: all fluxed up. Circ Res. 2015;116:504-14 pubmed publisher
..We will examine several published studies where confusion arose in data interpretation, to illustrate the challenges. Finally, we will discuss methods to assess autophagy in vivo and in patients. ..
Stotland A, Gottlieb R. Î±-MHC MitoTimer mouse: In vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart. J Mol Cell Cardiol. 2016;90:53-8 pubmed publisher
..These mitochondria can be isolated and sorted from the heart by flow cytometry for further analysis. Initial studies suggest that these mice represent an elegant tool for the investigation of mitochondrial turnover in the heart. ..
Gottlieb R, Bernstein D. Mitochondrial remodeling: Rearranging, recycling, and reprogramming. Cell Calcium. 2016;60:88-101 pubmed publisher
..The complex interplay of mitochondria, cytosolic factors, and the nucleus ensure a well-coordinated response to environmental stresses. ..
Gottlieb R, Pourpirali S. Lost in translation: miRNAs and mRNAs in ischemic preconditioning and ischemia/reperfusion injury. J Mol Cell Cardiol. 2016;95:70-7 pubmed publisher
..This review will explore some of the recent advances in our understanding of autophagy and mitochondrial turnover in the setting of I/R injury, and related findings drawn from research on hibernating extremophiles. ..