Jean F Welter

Summary

Affiliation: Case Western Reserve University
Country: USA

Publications

  1. pmc Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay
    Jean F Welter
    Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA
    Biotechniques 42:732, 734-7. 2007
  2. pmc Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Division of Hematology and Oncology, Department of General Medical Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106 7080, USA
    Tissue Eng Part A 16:1009-19. 2010
  3. ncbi request reprint FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    J Cell Physiol 203:398-409. 2005
  4. ncbi request reprint Hydrostatic pressure increases apoptosis in cartilage-constructs produced from human osteoarthritic chondrocytes
    Reid Wenger
    Department of Orthodontics, Case Western Reserve University School of Dentistry, Cleveland, Ohio 44106 4946, USA
    Front Biosci 11:1690-5. 2006
  5. pmc Nondestructive evaluation of hydrogel mechanical properties using ultrasound
    Jason M Walker
    Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
    Ann Biomed Eng 39:2521-30. 2011
  6. pmc Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricks
    Luis A Solchaga
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
    Methods Mol Biol 698:253-78. 2011
  7. pmc High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cells
    Kitsie J Penick
    Case Western Reserve University, Cleveland, OH 44106 7080, USA
    Biotechniques 39:687-91. 2005
  8. ncbi request reprint Performance of polyoxymethylene plastic (POM) as a component of a tissue engineering bioreactor
    Kitsie J Penick
    Department of Biology Skeletal Research Center, Case Western Reserve University, Cleveland, OH 44106, USA
    J Biomed Mater Res A 75:168-74. 2005
  9. pmc Concentrated collagen-chondroitin sulfate scaffolds for tissue engineering applications
    Wan Hsiang Liang
    Department of Chemical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biomed Mater Res A 94:1050-60. 2010
  10. pmc Cartilage tissue engineering for laryngotracheal reconstruction: comparison of chondrocytes from three anatomic locations in the rabbit
    James H Henderson
    Departments of Biology and Orthopaedics, Skeletal Research Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Tissue Eng 13:843-53. 2007

Collaborators

Detail Information

Publications21

  1. pmc Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay
    Jean F Welter
    Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA
    Biotechniques 42:732, 734-7. 2007
    ..Thus, it appears safe to eliminate the centrifugation step from the aggregate culture protocol. This results in significant time and effort savings and paves the way for future full automation of the aggregate assay...
  2. pmc Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Division of Hematology and Oncology, Department of General Medical Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106 7080, USA
    Tissue Eng Part A 16:1009-19. 2010
    ..Supplementation with FGF-2 allows reaching target cell numbers more rapidly and extends the level of expansion within which these cells are useful for tissue-engineered cartilage repair...
  3. ncbi request reprint FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    J Cell Physiol 203:398-409. 2005
    ..With the generation of gene expression profiles of FGF-treated and control cells we have taken the first steps in the elucidation of the molecular mechanism(s) behind these phenomena...
  4. ncbi request reprint Hydrostatic pressure increases apoptosis in cartilage-constructs produced from human osteoarthritic chondrocytes
    Reid Wenger
    Department of Orthodontics, Case Western Reserve University School of Dentistry, Cleveland, Ohio 44106 4946, USA
    Front Biosci 11:1690-5. 2006
    ..02) the proportion of apoptotic cells in the cartilage-constructs (41% +/- 4.2%; mean +/- SD) compared to control cartilage-constructs (28.5 +/- 8.4%)...
  5. pmc Nondestructive evaluation of hydrogel mechanical properties using ultrasound
    Jason M Walker
    Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
    Ann Biomed Eng 39:2521-30. 2011
    ..A stronger correlation with mechanical measurements was found using the poroelastic rather than the elastic model. Results from this preliminary testing will be used to guide further studies of native and engineered cartilage...
  6. pmc Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricks
    Luis A Solchaga
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
    Methods Mol Biol 698:253-78. 2011
    ....
  7. pmc High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cells
    Kitsie J Penick
    Case Western Reserve University, Cleveland, OH 44106 7080, USA
    Biotechniques 39:687-91. 2005
    ..When analyzed histologically, both systems demonstrate morphological characteristics that are consistent with chondrogenic differentiation and cartilaginous extracellular matrix production...
  8. ncbi request reprint Performance of polyoxymethylene plastic (POM) as a component of a tissue engineering bioreactor
    Kitsie J Penick
    Department of Biology Skeletal Research Center, Case Western Reserve University, Cleveland, OH 44106, USA
    J Biomed Mater Res A 75:168-74. 2005
    ..The contrast of these findings to those of LaIuppa et al.5 may reflect a cell-type specific sensitivity, or may be due to different handling of the material...
  9. pmc Concentrated collagen-chondroitin sulfate scaffolds for tissue engineering applications
    Wan Hsiang Liang
    Department of Chemical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biomed Mater Res A 94:1050-60. 2010
    ..In addition, our tissue culture results with human mesenchymal stem cells and foreskin keratinocytes show that the new scaffolds can be used for cartilage and skin tissue-engineering applications...
  10. pmc Cartilage tissue engineering for laryngotracheal reconstruction: comparison of chondrocytes from three anatomic locations in the rabbit
    James H Henderson
    Departments of Biology and Orthopaedics, Skeletal Research Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Tissue Eng 13:843-53. 2007
    ..The results also suggest that this and similar tissue engineering approaches must be optimized for each potential source of chondrocytes...
  11. pmc Micrometer scale guidance of mesenchymal stem cells to form structurally oriented cartilage extracellular matrix
    Chih Ling Chou
    1 Department of Chemical Engineering, Case Western Reserve University, Cleveland, Ohio
    Tissue Eng Part A 19:1081-90. 2013
    ..These findings can be used to fabricate large clinically useful MSC-cartilage constructs with superior mechanical properties...
  12. pmc Primary cilia modulate Ihh signal transduction in response to hydrostatic loading of growth plate chondrocytes
    Yvonne Y Shao
    Department of Biomedical Engineering, Cleveland Clinic, Cleveland, OH 44195, USA
    Bone 50:79-84. 2012
    ..These results suggest that growth plate chondrocytes respond to hydrostatic loading by increasing Ihh signaling, and that the primary cilium is required for this mechano-biological signal transduction to occur...
  13. ncbi request reprint Evaluation of machining methods for trabecular metal implants in a rabbit intramedullary osseointegration model
    Mukund Deglurkar
    Department of Orthopaedics, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biomed Mater Res B Appl Biomater 80:528-40. 2007
    ..The resulting mechanical properties of the bone implant-interface were similar in all three groups, with only interface stiffness and interface shear modulus being significantly higher in the machined samples...
  14. pmc Assessing adipogenic potential of mesenchymal stem cells: a rapid three-dimensional culture screening technique
    Jean F Welter
    Department of Biology, Skeletal Research Center, Case Western Reserve University, 2080 Adelbert Road, Millis Science Center, Room 112A, 2080 Adelbert Rood, Cleveland, OH 44106 7080, USA
    Stem Cells Int 2013:806525. 2013
    ..As a differentiation assay, this method can distinguish between degrees of senescence and appears suitable for testing medium or drug formulations in a high-volume, high-throughput fashion...
  15. ncbi request reprint High-efficiency nonviral transfection of primary chondrocytes
    Jean F Welter
    Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
    Methods Mol Med 100:129-46. 2004
    ..In addition to the primary protocol, we present two additional reliable, albeit less efficient backup protocols, the first using exponential decay electroporation and the second FuGENE 6 transfection...
  16. ncbi request reprint Hydrostatic pressure induces apoptosis in human chondrocytes from osteoarthritic cartilage through up-regulation of tumor necrosis factor-alpha, inducible nitric oxide synthase, p53, c-myc, and bax-alpha, and suppression of bcl-2
    Najmul Islam
    Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4946, USA
    J Cell Biochem 87:266-78. 2002
    ..These results indicated that HP is a strong inducer of apoptosis in osteoarthritic human chondrocytes in vitro...
  17. pmc Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential
    Jeffery J Auletta
    Divisions of Pediatric Hematology Oncology and Pediatric Infectious Diseases, Department of Pediatrics, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
    Stem Cells Int 2011:235176. 2011
    ..However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion...
  18. pmc Combined experimental and mathematical approach for development of microfabrication-based cancer migration assay
    Saheli Sarkar
    Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
    Ann Biomed Eng 39:2346-59. 2011
    ..The combined experimental and mathematical model can be used to quantitatively study the impact of cell-cell interactions on migration...
  19. ncbi request reprint Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotype
    Luis A Solchaga
    Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
    Methods Mol Med 100:53-68. 2004
    ..Although we have the technology to accomplish chondrogenic differentiation of stem cells, much is still to be learned regarding the regulatory mechanisms controlling the lineage transitions and maturation of the cartilaginous tissue...
  20. ncbi request reprint Sequence, gene structure, and expression pattern of CTNNBL1, a minor-class intron-containing gene--evidence for a role in apoptosis
    Leila Jabbour
    Department of Anatomy, Case Western Reserve University, Cleveland, OH 44106, USA
    Genomics 81:292-303. 2003
    ..The rate of cell death was higher when cells were transfected with a carboxy-terminal fragment of CTNNBL1, suggesting that the apoptosis-inducing activity is a function of this region...
  21. pmc A rapid seeding technique for the assembly of large cell/scaffold composite constructs
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University Cleveland, Ohio 44106 7080, USA
    Tissue Eng 12:1851-63. 2006
    ..The vacuum seeding technique is safe, rapid, reproducible, and results in controlled quantitative cell loading, high retention, and uniform distribution...