Research Topics
| Saba ValadkhanSummaryAffiliation: Case Western Reserve University Country: USA Publications
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Detail Information
Publications
A snRNP's ordered path to maturitySaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
Genes Dev 25:1563-7. 2011..1601-1612) shed light on the dynamic assembly of this critical spliceosomal component and elucidate the molecular interactions underlying the ordered addition of Brr2, a pivotal spliceosomal helicase, to the U5 snRNP...
Role of the snRNAs in spliceosomal active siteSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH, USA
RNA Biol 7:345-53. 2010..Thus, whether the spliceosome is an RNA or RNA-protein catalyst remains uncertain...
Reprogramming of the non-coding transcriptome during brain developmentSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106, USA
J Biol 9:5. 2010....
Protein-free small nuclear RNAs catalyze a two-step splicing reactionSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
Proc Natl Acad Sci U S A 106:11901-6. 2009..These results prove that the complex formed by U6 and U2 RNAs is a ribozyme and can potentially carry out RNA-based catalysis in the spliceosome...
The spliceosomal proteome: at the heart of the largest cellular ribonucleoprotein machineSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44113, USA
Proteomics 10:4128-41. 2010....
Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicingSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
RNA 13:2300-11. 2007..Our results demonstrate the ability of protein-free snRNAs to recognize the sequences involved in the first splicing step and to perform splicing-related catalysis between these two pre-mRNA-like substrates...
The spliceosome: caught in a web of shifting interactionsSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University, 10900 Euclid Avenue, Wood RT 100 8, Cleveland, OH 44106, USA
Curr Opin Struct Biol 17:310-5. 2007..Overall, the past two years have seen a convergence of data from different lines of research into what promises to become a holistic picture of spliceosome function...
The spliceosome: a ribozyme at heart?Saba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106, USA
Biol Chem 388:693-7. 2007..Of the five spliceosomal RNAs, U2 and U6 are the only ones that are absolutely required for both steps of splicing. These two snRNAs form an elaborate base-paired complex that might in fact constitute the active site of the spliceosome...
snRNAs as the catalysts of pre-mRNA splicingSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
Curr Opin Chem Biol 9:603-8. 2005..An emerging model for the active site of group II introns, a closely related class of natural ribozymes, is likely to provide a wealth of insights on structure and function of the active site of the spliceosome...
Young Scientist Award essay winner. Construction of a minimal, protein-free spliceosomeSaba Valadkhan
Case Western University, 2109 Adelbert Road, Wood RT 100-8, Cleveland, OH 44106, USA
Science 307:863-4. 2005
Metal binding and substrate positioning by evolutionarily invariant U6 sequences in catalytically active protein-free snRNAsCaroline Lee
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
RNA 16:2226-38. 2010....
Splicing of an intervening sequence by protein-free human snRNAsYasaman Jaladat
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH, USA
RNA Biol 8:372-7. 2011..These results prove that the protein-free snRNAs are competent to perform splicing on pre-mRNAs and further strengthen the possibility of an evolutionary relationship to group II introns...
The use of simple model systems to study spliceosomal catalysisSaba Valadkhan
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
RNA 15:4-7. 2009..Furthermore, they strongly suggest that with further optimization, these RNAs might indeed be able to recapitulate the function of the spliceosomal catalytic core, thus opening the door to several lines of study not previously possible...
Characterization of the catalytic activity of U2 and U6 snRNAsSaba Valadkhan
Department of Biological Sciences, Columbia University, New York, New York 10027, USA
RNA 9:892-904. 2003..Our data suggest that the ability to form RNA X might be an intrinsic property of spliceosomal snRNAs...
Intrinsic metal binding by a spliceosomal RNASaba Valadkhan
Nat Struct Biol 9:498-9. 2002
