Luis A Solchaga

Summary

Affiliation: Case Western Reserve University
Country: USA

Publications

  1. pmc Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Division of Hematology and Oncology, Department of General Medical Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106 7080, USA
    Tissue Eng Part A 16:1009-19. 2010
  2. ncbi request reprint FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    J Cell Physiol 203:398-409. 2005
  3. pmc A rapid seeding technique for the assembly of large cell/scaffold composite constructs
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University Cleveland, Ohio 44106 7080, USA
    Tissue Eng 12:1851-63. 2006
  4. ncbi request reprint Hydrostatic pressure increases apoptosis in cartilage-constructs produced from human osteoarthritic chondrocytes
    Reid Wenger
    Department of Orthodontics, Case Western Reserve University School of Dentistry, Cleveland, Ohio 44106 4946, USA
    Front Biosci 11:1690-5. 2006
  5. ncbi request reprint Repair of osteochondral defects with hyaluronan- and polyester-based scaffolds
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, OH, USA
    Osteoarthritis Cartilage 13:297-309. 2005
  6. ncbi request reprint Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotype
    Luis A Solchaga
    Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
    Methods Mol Med 100:53-68. 2004
  7. ncbi request reprint Performance of polyoxymethylene plastic (POM) as a component of a tissue engineering bioreactor
    Kitsie J Penick
    Department of Biology Skeletal Research Center, Case Western Reserve University, Cleveland, OH 44106, USA
    J Biomed Mater Res A 75:168-74. 2005
  8. pmc High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cells
    Kitsie J Penick
    Case Western Reserve University, Cleveland, OH 44106 7080, USA
    Biotechniques 39:687-91. 2005
  9. ncbi request reprint Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable calcium phosphate and hyaluronan sponge
    Jizong Gao
    Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, Cleveland, OH 44106, USA
    Tissue Eng 8:827-37. 2002
  10. pmc Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricks
    Luis A Solchaga
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
    Methods Mol Biol 698:253-78. 2011

Collaborators

Detail Information

Publications18

  1. pmc Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Division of Hematology and Oncology, Department of General Medical Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106 7080, USA
    Tissue Eng Part A 16:1009-19. 2010
    ..Supplementation with FGF-2 allows reaching target cell numbers more rapidly and extends the level of expansion within which these cells are useful for tissue-engineered cartilage repair...
  2. ncbi request reprint FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    J Cell Physiol 203:398-409. 2005
    ..With the generation of gene expression profiles of FGF-treated and control cells we have taken the first steps in the elucidation of the molecular mechanism(s) behind these phenomena...
  3. pmc A rapid seeding technique for the assembly of large cell/scaffold composite constructs
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University Cleveland, Ohio 44106 7080, USA
    Tissue Eng 12:1851-63. 2006
    ..The vacuum seeding technique is safe, rapid, reproducible, and results in controlled quantitative cell loading, high retention, and uniform distribution...
  4. ncbi request reprint Hydrostatic pressure increases apoptosis in cartilage-constructs produced from human osteoarthritic chondrocytes
    Reid Wenger
    Department of Orthodontics, Case Western Reserve University School of Dentistry, Cleveland, Ohio 44106 4946, USA
    Front Biosci 11:1690-5. 2006
    ..02) the proportion of apoptotic cells in the cartilage-constructs (41% +/- 4.2%; mean +/- SD) compared to control cartilage-constructs (28.5 +/- 8.4%)...
  5. ncbi request reprint Repair of osteochondral defects with hyaluronan- and polyester-based scaffolds
    Luis A Solchaga
    Department of Orthopaedics, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, OH, USA
    Osteoarthritis Cartilage 13:297-309. 2005
    ....
  6. ncbi request reprint Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotype
    Luis A Solchaga
    Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
    Methods Mol Med 100:53-68. 2004
    ..Although we have the technology to accomplish chondrogenic differentiation of stem cells, much is still to be learned regarding the regulatory mechanisms controlling the lineage transitions and maturation of the cartilaginous tissue...
  7. ncbi request reprint Performance of polyoxymethylene plastic (POM) as a component of a tissue engineering bioreactor
    Kitsie J Penick
    Department of Biology Skeletal Research Center, Case Western Reserve University, Cleveland, OH 44106, USA
    J Biomed Mater Res A 75:168-74. 2005
    ..The contrast of these findings to those of LaIuppa et al.5 may reflect a cell-type specific sensitivity, or may be due to different handling of the material...
  8. pmc High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cells
    Kitsie J Penick
    Case Western Reserve University, Cleveland, OH 44106 7080, USA
    Biotechniques 39:687-91. 2005
    ..When analyzed histologically, both systems demonstrate morphological characteristics that are consistent with chondrogenic differentiation and cartilaginous extracellular matrix production...
  9. ncbi request reprint Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable calcium phosphate and hyaluronan sponge
    Jizong Gao
    Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, Cleveland, OH 44106, USA
    Tissue Eng 8:827-37. 2002
    ....
  10. pmc Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricks
    Luis A Solchaga
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
    Methods Mol Biol 698:253-78. 2011
    ....
  11. pmc Transcriptional profiling of human mesenchymal stem cells transduced with reporter genes for imaging
    Fangjing Wang
    Department of Biomedical Engineering, University Hospitals, Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA
    Physiol Genomics 37:23-34. 2009
    ..In conclusion, transduction of reporter genes into MSCs preserved the basic properties of stem cells while enabling noninvasive imaging in living animals to study the biodistribution and other biological activities of the cells...
  12. pmc Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay
    Jean F Welter
    Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA
    Biotechniques 42:732, 734-7. 2007
    ..Thus, it appears safe to eliminate the centrifugation step from the aggregate culture protocol. This results in significant time and effort savings and paves the way for future full automation of the aggregate assay...
  13. doi request reprint Regenerative stromal cell therapy in allogeneic hematopoietic stem cell transplantation: current impact and future directions
    Jeffery J Auletta
    Rainbow Babies and Children s Hospital, Cleveland, OH, USA
    Biol Blood Marrow Transplant 16:891-906. 2010
    ....
  14. ncbi request reprint Treatment of osteochondral defects with autologous bone marrow in a hyaluronan-based delivery vehicle
    Luis A Solchaga
    Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, Ohio, USA
    Tissue Eng 8:333-47. 2002
    ..The fundamental tissue engineering principles derived from this study should provide guidelines for the development of comparable clinical reconstructive therapies...
  15. pmc Assessing adipogenic potential of mesenchymal stem cells: a rapid three-dimensional culture screening technique
    Jean F Welter
    Department of Biology, Skeletal Research Center, Case Western Reserve University, 2080 Adelbert Road, Millis Science Center, Room 112A, 2080 Adelbert Rood, Cleveland, OH 44106 7080, USA
    Stem Cells Int 2013:806525. 2013
    ..As a differentiation assay, this method can distinguish between degrees of senescence and appears suitable for testing medium or drug formulations in a high-volume, high-throughput fashion...
  16. ncbi request reprint High-efficiency nonviral transfection of primary chondrocytes
    Jean F Welter
    Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
    Methods Mol Med 100:129-46. 2004
    ..In addition to the primary protocol, we present two additional reliable, albeit less efficient backup protocols, the first using exponential decay electroporation and the second FuGENE 6 transfection...
  17. pmc Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential
    Jeffery J Auletta
    Divisions of Pediatric Hematology Oncology and Pediatric Infectious Diseases, Department of Pediatrics, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
    Stem Cells Int 2011:235176. 2011
    ..However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion...
  18. ncbi request reprint Bioreactors mediate the effectiveness of tissue engineering scaffolds
    Ming Pei
    Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    FASEB J 16:1691-4. 2002
    ..The data imply that interactions between bioreactors and 3D tissue engineering scaffolds can be utilized to improve the structure, function, and molecular properties of in vitro-generated cartilage...