Research Topics
| Luis A SolchagaSummaryAffiliation: Case Western Reserve University Country: USA Publications
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Detail Information
Publications
Fibroblast growth factor-2 enhances proliferation and delays loss of chondrogenic potential in human adult bone-marrow-derived mesenchymal stem cellsLuis A Solchaga
Division of Hematology and Oncology, Department of General Medical Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106 7080, USA
Tissue Eng Part A 16:1009-19. 2010..Supplementation with FGF-2 allows reaching target cell numbers more rapidly and extends the level of expansion within which these cells are useful for tissue-engineered cartilage repair...
FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cellsLuis A Solchaga
Department of Orthopaedics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
J Cell Physiol 203:398-409. 2005..With the generation of gene expression profiles of FGF-treated and control cells we have taken the first steps in the elucidation of the molecular mechanism(s) behind these phenomena...- A rapid seeding technique for the assembly of large cell/scaffold composite constructsLuis A Solchaga
Department of Orthopaedics, Case Western Reserve University Cleveland, Ohio 44106-7080, USA
Tissue Eng 12:1851-63. 2006..The vacuum seeding technique is safe, rapid, reproducible, and results in controlled quantitative cell loading, high retention, and uniform distribution... - Repair of osteochondral defects with hyaluronan- and polyester-based scaffoldsLuis A Solchaga
Department of Orthopaedics, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, OH, USA
Osteoarthritis Cartilage 13:297-309. 2005.... - Hydrostatic pressure increases apoptosis in cartilage-constructs produced from human osteoarthritic chondrocytesReid Wenger
Department of Orthodontics, Case Western Reserve University School of Dentistry, Cleveland, Ohio 44106-4946, USA
Front Biosci 11:1690-5. 2006..02) the proportion of apoptotic cells in the cartilage-constructs (41% +/- 4.2%; mean +/- SD) compared to control cartilage-constructs (28.5 +/- 8.4%)... - Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotypeLuis A Solchaga
Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
Methods Mol Med 100:53-68. 2004..Although we have the technology to accomplish chondrogenic differentiation of stem cells, much is still to be learned regarding the regulatory mechanisms controlling the lineage transitions and maturation of the cartilaginous tissue... - Performance of polyoxymethylene plastic (POM) as a component of a tissue engineering bioreactorKitsie J Penick
Department of Biology (Skeletal Research Center, Case Western Reserve University, Cleveland, OH 44106, USA
J Biomed Mater Res A 75:168-74. 2005..The contrast of these findings to those of LaIuppa et al.5 may reflect a cell-type specific sensitivity, or may be due to different handling of the material... - High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cellsKitsie J Penick
Case Western Reserve University, Cleveland, OH 44106-7080, USA
Biotechniques 39:687-91. 2005..When analyzed histologically, both systems demonstrate morphological characteristics that are consistent with chondrogenic differentiation and cartilaginous extracellular matrix production... - Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable calcium phosphate and hyaluronan spongeJizong Gao
Skeletal Research Center, Department of Biology, Case Western Reserve University, 2080 Adelbert Road, Cleveland, OH 44106, USA
Tissue Eng 8:827-37. 2002.... - Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells: tips and tricksLuis A Solchaga
Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
Methods Mol Biol 698:253-78. 2011.... - Transcriptional profiling of human mesenchymal stem cells transduced with reporter genes for imagingFangjing Wang
Department of Biomedical Engineering, University Hospitals, Case Medical Center, Case Western Reserve University, Cleveland, Ohio, USA
Physiol Genomics 37:23-34. 2009..In conclusion, transduction of reporter genes into MSCs preserved the basic properties of stem cells while enabling noninvasive imaging in living animals to study the biodistribution and other biological activities of the cells... - Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assayJean F Welter
Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA
Biotechniques 42:732, 734-7. 2007..Thus, it appears safe to eliminate the centrifugation step from the aggregate culture protocol. This results in significant time and effort savings and paves the way for future full automation of the aggregate assay... 
Regenerative stromal cell therapy in allogeneic hematopoietic stem cell transplantation: current impact and future directionsJeffery J Auletta
Rainbow Babies and Children s Hospital, Cleveland, OH, USA
Biol Blood Marrow Transplant 16:891-906. 2010....- Treatment of osteochondral defects with autologous bone marrow in a hyaluronan-based delivery vehicleLuis A Solchaga
Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, Ohio, USA
Tissue Eng 8:333-47. 2002..The fundamental tissue engineering principles derived from this study should provide guidelines for the development of comparable clinical reconstructive therapies... - High-efficiency nonviral transfection of primary chondrocytesJean F Welter
Department of Orthopedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH, USA
Methods Mol Med 100:129-46. 2004..In addition to the primary protocol, we present two additional reliable, albeit less efficient backup protocols, the first using exponential decay electroporation and the second FuGENE 6 transfection... - Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive PotentialJeffery J Auletta
Divisions of Pediatric Hematology Oncology and Pediatric Infectious Diseases, Department of Pediatrics, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
Stem Cells Int 2011:235176. 2011..However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion... - Bioreactors mediate the effectiveness of tissue engineering scaffoldsMing Pei
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
FASEB J 16:1691-4. 2002..The data imply that interactions between bioreactors and 3D tissue engineering scaffolds can be utilized to improve the structure, function, and molecular properties of in vitro-generated cartilage...