Rajesh Ramachandran

Summary

Affiliation: Case Western Reserve University
Country: USA

Publications

  1. doi request reprint Vesicle scission: dynamin
    Rajesh Ramachandran
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    Semin Cell Dev Biol 22:10-7. 2011
  2. pmc Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:4630-9. 2009
  3. pmc The dynamin middle domain is critical for tetramerization and higher-order self-assembly
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:559-66. 2007
  4. pmc Differential curvature sensing and generating activities of dynamin isoforms provide opportunities for tissue-specific regulation
    Ya Wen Liu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:E234-42. 2011
  5. pmc Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membrane
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 27:27-37. 2008
  6. ncbi request reprint Robust colorimetric assays for dynamin's basal and stimulated GTPase activities
    Marilyn Leonard
    The Scripps Research Institute, Department of Cell Biology, La Jolla, California, USA
    Methods Enzymol 404:490-503. 2005
  7. pmc Dissecting dynamin's role in clathrin-mediated endocytosis
    Marcel Mettlen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochem Soc Trans 37:1022-6. 2009
  8. pmc Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells
    Prattana Samasilp
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio and
    Am J Physiol Cell Physiol 306:C831-43. 2014
  9. pmc Alternate pleckstrin homology domain orientations regulate dynamin-catalyzed membrane fission
    Niharika Mehrotra
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106
    Mol Biol Cell 25:879-90. 2014
  10. pmc A dimeric equilibrium intermediate nucleates Drp1 reassembly on mitochondrial membranes for fission
    Patrick J MacDonald
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106
    Mol Biol Cell 25:1905-15. 2014

Collaborators

Detail Information

Publications10

  1. doi request reprint Vesicle scission: dynamin
    Rajesh Ramachandran
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    Semin Cell Dev Biol 22:10-7. 2011
    ..Coordinated functions of BAR domain-containing binding partners are also discussed...
  2. pmc Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:4630-9. 2009
    ..These results demonstrate that, in concert with dynamin self-assembly, PH domain membrane insertion is essential for fission and vesicle release in vitro and for clathrin-mediated endocytosis in vivo...
  3. pmc The dynamin middle domain is critical for tetramerization and higher-order self-assembly
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:559-66. 2007
    ..Consequently, these mutations also impair dynamin's assembly-dependent stimulated GTPase activity...
  4. pmc Differential curvature sensing and generating activities of dynamin isoforms provide opportunities for tissue-specific regulation
    Ya Wen Liu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:E234-42. 2011
    ..Our findings reveal opportunities for fundamental differences in the regulation of Dyn1, which mediates rapid endocytosis at the synapse, vs. Dyn2, which regulates early and late events in CME in nonneuronal cells...
  5. pmc Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membrane
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 27:27-37. 2008
    ..Amphiphysin I has the opposite effect. Thus, dynamin's mechanochemical properties on a membrane surface are dynamically regulated by its GTPase cycle and major binding partners...
  6. ncbi request reprint Robust colorimetric assays for dynamin's basal and stimulated GTPase activities
    Marilyn Leonard
    The Scripps Research Institute, Department of Cell Biology, La Jolla, California, USA
    Methods Enzymol 404:490-503. 2005
    ..We describe this assay and report variations in assay conditions that have contributed to the wide range of reported values for dynamin's basal and assembly-stimulated rates of GTP hydrolysis...
  7. pmc Dissecting dynamin's role in clathrin-mediated endocytosis
    Marcel Mettlen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochem Soc Trans 37:1022-6. 2009
    ....
  8. pmc Syndapin 3 modulates fusion pore expansion in mouse neuroendocrine chromaffin cells
    Prattana Samasilp
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio and
    Am J Physiol Cell Physiol 306:C831-43. 2014
    ..The mutation also disrupts targeting of syndapin 3 to the cell periphery. Syndapin 3 exists in a persistent colocalized state with dynamin 1...
  9. pmc Alternate pleckstrin homology domain orientations regulate dynamin-catalyzed membrane fission
    Niharika Mehrotra
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106
    Mol Biol Cell 25:879-90. 2014
    ..Stabilization of this alternate orientation produces dramatic variations in the morphology of membrane-bound dynamin spirals, indicating that the PH domain regulates membrane fission through the control of dynamin polymer dynamics. ..
  10. pmc A dimeric equilibrium intermediate nucleates Drp1 reassembly on mitochondrial membranes for fission
    Patrick J MacDonald
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106
    Mol Biol Cell 25:1905-15. 2014
    ....