Irina A Pikuleva
Affiliation: Case Western Reserve University
Saadane A, Petrov A, Mast N, El Darzi N, Dao T, Alnemri A, et al
. Mechanisms that minimize retinal impact of apolipoprotein E absence. J Lipid Res. 2018;: pubmed publisher
..These and other compensatory changes underlie only a minor retinal phenotype in Apoe-/- mice. ..
Mast N, Bederman I, Pikuleva I. Retinal Cholesterol Content Is Reduced in Simvastatin-Treated Mice Due to Inhibited Local Biosynthesis Albeit Increased Uptake of Serum Cholesterol. Drug Metab Dispos. 2018;46:1528-1537 pubmed publisher
..Our results reveal the statin effect on the retinal and brain cholesterol input and are of value for future clinical investigations of statins as potential therapeutics for age-related macular degeneration. ..
Pikuleva I, Waterman M. Cytochromes p450: roles in diseases. J Biol Chem. 2013;288:17091-8 pubmed publisher
..In this minireview, we describe medically significant human P450s (from families 2, 4, 7, 11, 17, 19, 21, 24, 27, 46, and 51) and the diseases associated with these P450s. ..
Mast N, Lin J, Pikuleva I. Marketed Drugs Can Inhibit Cytochrome P450 27A1, a Potential New Target for Breast Cancer Adjuvant Therapy. Mol Pharmacol. 2015;88:428-36 pubmed publisher
..More medications on the US market may have unanticipated off-target inhibition of CYP27A1, and we propose strategies for their identification. ..
van Lier J, Mast N, Pikuleva I. Cholesterol hydroperoxides as substrates for cholesterol-metabolizing cytochromeâ€…P450 enzymes and alternative sources of 25-hydroxycholesterol and other oxysterols. Angew Chem Int Ed Engl. 2015;54:11138-42 pubmed publisher
..CYP27A1 may participate in the removal of harmful autoxidation products in these tissues, while providing a complementary source of 25-hydroxycholesterol, a modulator of immune cell function and mediator of viral cell entry. ..
Lin J, Mast N, Bederman I, Li Y, Brunengraber H, BjÃ¶rkhem I, et al
. Cholesterol in mouse retina originates primarily from in situ de novo biosynthesis. J Lipid Res. 2016;57:258-64 pubmed publisher
Anderson K, Mast N, Hudgens J, Lin J, Turko I, Pikuleva I. Mapping of the Allosteric Site in Cholesterol Hydroxylase CYP46A1 for Efavirenz, a Drug That Stimulates Enzyme Activity. J Biol Chem. 2016;291:11876-86 pubmed publisher
..On the basis of the data obtained, we propose the mechanism of CYP46A1 allostery and the pathway for the signal transmission from the P450 allosteric site to the active site. ..
Saadane A, Mast N, Charvet C, Omarova S, Zheng W, Huang S, et al
. Retinal and nonocular abnormalities in Cyp27a1(-/-)Cyp46a1(-/-) mice with dysfunctional metabolism of cholesterol. Am J Pathol. 2014;184:2403-19 pubmed publisher
..Our findings demonstrate the importance of retinal cholesterol metabolism for maintenance of the normal retina, and suggest new targets for diseases affecting the retinal vasculature. ..
Mast N, Saadane A, Valencia Olvera A, Constans J, Maxfield E, Arakawa H, et al
. Cholesterol-metabolizing enzyme cytochrome P450 46A1 as a pharmacologic target for Alzheimer's disease. Neuropharmacology. 2017;123:465-476 pubmed publisher
..We suggest that CYP46A1 activation by efavirenz could be a new anti-Alzheimer's disease treatment and a tool to study and identify normal and pathological brain processes affected by cholesterol maintenance. ..