Krzysztof Palczewski

Summary

Affiliation: Case Western Reserve University
Country: USA

Publications

  1. pmc G protein-coupled receptor rhodopsin
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Annu Rev Biochem 75:743-67. 2006
  2. pmc Chemistry and biology of vision
    Krzysztof Palczewski
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 287:1612-9. 2012
  3. pmc Primary amines protect against retinal degeneration in mouse models of retinopathies
    Akiko Maeda
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio, USA
    Nat Chem Biol 8:170-8. 2012
  4. pmc Photoreceptor phagocytosis is mediated by phosphoinositide signaling
    Debarshi Mustafi
    1Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106 4965, USA
    FASEB J 27:4585-95. 2013
  5. doi request reprint From atomic structures to neuronal functions of g protein-coupled receptors
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Annu Rev Neurosci 36:139-64. 2013
  6. pmc Asymmetry of the rhodopsin dimer in complex with transducin
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    FASEB J 27:1572-84. 2013
  7. pmc Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base
    Lucy Skrabanek
    Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, NY, USA
    BMC Bioinformatics 8:177. 2007
  8. pmc Oligomeric forms of G protein-coupled receptors (GPCRs)
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Trends Biochem Sci 35:595-600. 2010
  9. pmc Defective photoreceptor phagocytosis in a mouse model of enhanced S-cone syndrome causes progressive retinal degeneration
    Debarshi Mustafi
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    FASEB J 25:3157-76. 2011
  10. pmc Retinoids for treatment of retinal diseases
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Trends Pharmacol Sci 31:284-95. 2010

Research Grants

  1. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2003
  2. STRUCTURAL STUDIES OF ARRESTINS
    Krzysztof Palczewski; Fiscal Year: 2000
  3. RETINOIDS IN VISION
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2010
  4. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2005
  5. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2005
  6. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2005
  7. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2002
  8. PHOTOTRANSDUCTION ENZYMES
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2010
  9. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2009
  10. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2006

Collaborators

Detail Information

Publications108 found, 100 shown here

  1. pmc G protein-coupled receptor rhodopsin
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Annu Rev Biochem 75:743-67. 2006
    ..The results of these investigations provide evidence of the propensity of rhodopsin and, most likely, other GPCRs to dimerize, a property that may be pertinent to their function...
  2. pmc Chemistry and biology of vision
    Krzysztof Palczewski
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 287:1612-9. 2012
    ..Moreover, other nonconventional retinal opsins such as the circadian rhythm regulator melanopsin also initiate light-activated signaling based on similar photochemistry...
  3. pmc Primary amines protect against retinal degeneration in mouse models of retinopathies
    Akiko Maeda
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio, USA
    Nat Chem Biol 8:170-8. 2012
    ....
  4. pmc Photoreceptor phagocytosis is mediated by phosphoinositide signaling
    Debarshi Mustafi
    1Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106 4965, USA
    FASEB J 27:4585-95. 2013
    ..Our phenotypic and genotypic characterization reveals a complex global landscape of overlapping and temporally controlled networks driving the essential circadian process in the eye...
  5. doi request reprint From atomic structures to neuronal functions of g protein-coupled receptors
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Annu Rev Neurosci 36:139-64. 2013
    ....
  6. pmc Asymmetry of the rhodopsin dimer in complex with transducin
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    FASEB J 27:1572-84. 2013
    ..This study provides a clear functional distinction between monomers of family A GPCRs in their oligomeric form...
  7. pmc Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base
    Lucy Skrabanek
    Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, NY, USA
    BMC Bioinformatics 8:177. 2007
    ....
  8. pmc Oligomeric forms of G protein-coupled receptors (GPCRs)
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Trends Biochem Sci 35:595-600. 2010
    ..Oligomerization of cell membrane receptors is fully supported by both thermodynamic calculations and the selectivity and duration of signaling required to reach targets located in various cellular compartments...
  9. pmc Defective photoreceptor phagocytosis in a mouse model of enhanced S-cone syndrome causes progressive retinal degeneration
    Debarshi Mustafi
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    FASEB J 25:3157-76. 2011
    ..Overcoming the retinal phagocytosis defect could arrest the progressive degenerative component of this disease...
  10. pmc Retinoids for treatment of retinal diseases
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Trends Pharmacol Sci 31:284-95. 2010
    ....
  11. pmc Phagocytosis of retinal rod and cone photoreceptors
    Brian M Kevany
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    Physiology (Bethesda) 25:8-15. 2010
    ..This review focuses on current knowledge of outer segment phagocytosis, discussing the steps involved along with their critical participants as well as how various perturbations in outer segment (OS) disposal can lead to retinopathies...
  12. pmc Focus on vision: 3 decades of remarkable contributions to biology and medicine
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106 4965, USA
    FASEB J 25:439-43. 2011
    ....
  13. pmc Metabolic basis of visual cycle inhibition by retinoid and nonretinoid compounds in the vertebrate retina
    Marcin Golczak
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    J Biol Chem 283:9543-54. 2008
    ..Finally, we provide evidence for a crucial role of lecithin: retinol acyltransferase activity in mediating tissue specific absorption and long lasting therapeutic effects of retinoid-based visual cycle inhibitors...
  14. pmc Autosomal recessive retinitis pigmentosa E150K opsin mice exhibit photoreceptor disorganization
    Ning Zhang
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44160, USA
    J Clin Invest 123:121-37. 2013
    ..Additionally, these results suggest that patients heterozygous for the E150K mutation should be periodically reevaluated for delayed-onset retinal degeneration...
  15. pmc Evaluation of potential therapies for a mouse model of human age-related macular degeneration caused by delayed all-trans-retinal clearance
    Tadao Maeda
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Invest Ophthalmol Vis Sci 50:4917-25. 2009
    ..Evaluate the efficacy of potential therapeutics in Rdh8(-/-)Abca4(-/-) mice, a rodent model of human age-related macular degeneration (AMD)...
  16. pmc Involvement of all-trans-retinal in acute light-induced retinopathy of mice
    Akiko Maeda
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    J Biol Chem 284:15173-83. 2009
    ..These findings further understanding of the mechanisms involved in light-induced retinal degeneration...
  17. pmc Limited roles of Rdh8, Rdh12, and Abca4 in all-trans-retinal clearance in mouse retina
    Akiko Maeda
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4965, USA
    Invest Ophthalmol Vis Sci 50:5435-43. 2009
    ..The protective roles were examined of the all-trans-RDHs, Rdh8 and Rdh12, and the ATP-binding cassette transporter Abca4, retinoid cycle enzymes involved in all-trans-retinal clearance...
  18. ncbi request reprint Molecular biology and analytical chemistry methods used to probe the retinoid cycle
    Marcin Golczak
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Methods Mol Biol 652:229-45. 2010
    ..Biochemical protocols combined with advanced mass spectrometry should facilitate fundamental biological studies of vision...
  19. pmc Modulation of molecular interactions and function by rhodopsin palmitylation
    Paul S H Park
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Biochemistry 48:4294-304. 2009
    ..The absence of palmitylation in rhodopsin therefore destabilizes the molecular interactions formed in the carboxyl terminal end of the receptor, which appears to hinder the activation of transducin by light-activated rhodopsin...
  20. ncbi request reprint Two-photon microscopy reveals early rod photoreceptor cell damage in light-exposed mutant mice
    Akiko Maeda
    Departments of Pharmacology and Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH 44106
    Proc Natl Acad Sci U S A 111:E1428-37. 2014
    ..Collectively, these findings suggest that for light-induced retinopathies in mice, rod photoreceptors are the primary site of toxic retinoid accumulation and degeneration, followed by secondary changes in the RPE. ..
  21. pmc A functional kinase homology domain is essential for the activity of photoreceptor guanylate cyclase 1
    Grzegorz Bereta
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 285:1899-908. 2010
    ..However, evidence is presented that autophosphorylation does not regulate GC1 activity, in contrast to phosphorylation of other members of this cyclase family...
  22. pmc Retinal cone and rod photoreceptor cells exhibit differential susceptibility to light-induced damage
    Kiichiro Okano
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH, USA
    J Neurochem 121:146-56. 2012
    ..These findings suggest that progression of retinal degeneration in Rdh8(-/-) Abca4(-/-) mice is affected by differential vulnerability of rods and cones to light...
  23. pmc Post-translational modifications of the serotonin type 4 receptor heterologously expressed in mouse rod cells
    David Salom
    Polgenix Inc, Cleveland, Ohio 44106, United States
    Biochemistry 51:214-24. 2012
    ....
  24. pmc Redundant and unique roles of retinol dehydrogenases in the mouse retina
    Akiko Maeda
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Proc Natl Acad Sci U S A 104:19565-70. 2007
    ..The results suggest that RDH8 and RDH12 are dispensable in support of the visual cycle but appear to be key components in clearance of free all-trans-retinal, thereby preventing A2E accumulation and photoreceptor cell death...
  25. pmc Palmitoylation stabilizes unliganded rod opsin
    Akiko Maeda
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Proc Natl Acad Sci U S A 107:8428-33. 2010
    ..Together, these observations provide evidence for a structural role of rhodopsin S-palmitoylation that may apply to other GPCRs as well...
  26. pmc Loss of cone photoreceptors caused by chromophore depletion is partially prevented by the artificial chromophore pro-drug, 9-cis-retinyl acetate
    Tadao Maeda
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4965, USA
    Hum Mol Genet 18:2277-87. 2009
    ..These results suggest that chronic lack of chromophore leads to progressive loss of cones in mice and humans. Therapy for LCA patients should be geared toward early adequate delivery of chromophore to cone photoreceptors...
  27. pmc Noninvasive multiphoton fluorescence microscopy resolves retinol and retinal condensation products in mouse eyes
    Grazyna Palczewska
    Polgenix, Cleveland, Ohio, USA
    Nat Med 16:1444-9. 2010
    ....
  28. pmc Toll-like receptor 3 is required for development of retinopathy caused by impaired all-trans-retinal clearance in mice
    Satomi Shiose
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 286:15543-55. 2011
    ..These findings demonstrate that endogenous products from degenerating retina stimulate TLR3 that causes cellular apoptosis and retinal inflammation and that loss of TLR3 protects mice from CORD...
  29. pmc Stereospecificity of retinol saturase: absolute configuration, synthesis, and biological evaluation of dihydroretinoids
    Alexander R Moise
    Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    J Am Chem Soc 130:1154-5. 2008
  30. pmc Impact of retinal disease-associated RPE65 mutations on retinoid isomerization
    Grzegorz Bereta
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 47:9856-65. 2008
    ....
  31. pmc Two carotenoid oxygenases contribute to mammalian provitamin A metabolism
    Jaume Amengual
    From the Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106
    J Biol Chem 288:34081-96. 2013
    ..In conclusion, our study provides evidence that mammals employ both carotenoid oxygenases to synthesize retinoids from provitamin A carotenoids. ..
  32. pmc ISX is a retinoic acid-sensitive gatekeeper that controls intestinal beta,beta-carotene absorption and vitamin A production
    Glenn P Lobo
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44160, USA
    FASEB J 24:1656-66. 2010
    ....
  33. pmc Light-sensitive coupling of rhodopsin and melanopsin to G(i/o) and G(q) signal transduction in Caenorhabditis elegans
    Pengxiu Cao
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    FASEB J 26:480-91. 2012
    ..These transgenic C. elegans models provide a unique way to study the consequences of G(i/o) and G(q) signaling in vivo with temporal and spatial precision and, by analogy, their relationship to human neuromotor function...
  34. pmc Mechanism of all-trans-retinal toxicity with implications for stargardt disease and age-related macular degeneration
    Yu Chen
    Department of Pharmacology and Ophthalmology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    J Biol Chem 287:5059-69. 2012
    ..A similar mechanism may be operative in human Stargardt disease and age-related macular degeneration...
  35. pmc Key enzymes of the retinoid (visual) cycle in vertebrate retina
    Philip D Kiser
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Biochim Biophys Acta 1821:137-51. 2012
    ..This article is part of a Special Issue entitled: Retinoid and Lipid Metabolism...
  36. pmc Probing mechanisms of photoreceptor degeneration in a new mouse model of the common form of autosomal dominant retinitis pigmentosa due to P23H opsin mutations
    Sanae Sakami
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 286:10551-67. 2011
    ..These results indicate that most synthesized P23H protein is degraded, and its retinal cytotoxicity is enhanced by lack of the 11-cis-retinal chromophore during rod outer segment development...
  37. pmc Structural basis for the acyltransferase activity of lecithin:retinol acyltransferase-like proteins
    Marcin Golczak
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 287:23790-807. 2012
    ..This study contributes to the enzymatic and structural understanding of HRAS-like tumor suppressor enzymes...
  38. pmc A mitochondrial enzyme degrades carotenoids and protects against oxidative stress
    Jaume Amengual
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44160, USA
    FASEB J 25:948-59. 2011
    ..Mammalian cells thus express a mitochondrial carotenoid-oxygenase that degrades carotenoids to protect these vital organelles...
  39. pmc Importance of membrane structural integrity for RPE65 retinoid isomerization activity
    Marcin Golczak
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 285:9667-82. 2010
    ..Thus, our results indicate that the enzymatic activity of native RPE65 strongly depends on its membrane binding and phospholipid environment...
  40. pmc Effects of long-term administration of 9-cis-retinyl acetate on visual function in mice
    Tadao Maeda
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Invest Ophthalmol Vis Sci 50:322-33. 2009
    ..Long-term effects of treatment with 9-cis-retinyl acetate (9-cis-R-Ac), an artificial retinoid prodrug, were tested on changes in rod and cone visual functions in mice...
  41. pmc Role of bulk water in hydrolysis of the rhodopsin chromophore
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    J Biol Chem 286:18930-7. 2011
    ..Moreover, small molecules (and presumably, water) enter the Rho structure from the cytoplasmic side of the membrane. Thus, this work indicates two distinct origins of water vital for Rho function...
  42. pmc Aberrant metabolites in mouse models of congenital blinding diseases: formation and storage of retinyl esters
    Akiko Maeda
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 45:4210-9. 2006
    ..The data presented here support the idea that 13-cis-retinyl esters are formed through an aberrant enzymatic isomerization process...
  43. ncbi request reprint R9AP overexpression alters phototransduction kinetics in iCre75 mice
    Thomas R Sundermeier
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio
    Invest Ophthalmol Vis Sci 55:1339-47. 2014
    ..Determine the impact of rod photoreceptor-specific expression of Cre recombinase on the kinetics of phototransduction in the mouse eye and identify changes in gene expression that underlie any observed phenotypic differences...
  44. ncbi request reprint Retinol dehydrogenase (RDH12) protects photoreceptors from light-induced degeneration in mice
    Akiko Maeda
    Department of Pharmacology and Ophthalmology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 281:37697-704. 2006
    ..Thus, severe visual impairments of individuals with null mutations in RDH12 may likely be caused by light damage(1)...
  45. pmc Photoreceptor proteins initiate microglial activation via Toll-like receptor 4 in retinal degeneration mediated by all-trans-retinal
    Hideo Kohno
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106 7286, USA
    J Biol Chem 288:15326-41. 2013
    ..This pathway is likely to represent an underlying common pathology in degenerative retinal disorders...
  46. pmc Structure of RPE65 isomerase in a lipidic matrix reveals roles for phospholipids and iron in catalysis
    Philip D Kiser
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 109:E2747-56. 2012
    ..These results support the hypothesis that the Lewis acidity of iron could be used to promote ester dissociation and generation of a carbocation intermediate required for retinoid isomerization...
  47. pmc Conformational dynamics of activation for the pentameric complex of dimeric G protein-coupled receptor and heterotrimeric G protein
    Tivadar Orban
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4988, USA
    Structure 20:826-40. 2012
    ..Thus, we provide direct evidence that in the critical step of signal amplification, Rho(∗) and G(t) exhibit dissimilar conformational changes when they are coupled in the (Rho(∗))(2)-G(t) complex...
  48. pmc Heterologous expression of functional G-protein-coupled receptors in Caenorhabditis elegans
    David Salom
    Polgenix, Inc, Cleveland, Ohio, USA
    FASEB J 26:492-502. 2012
    ..elegans system to produce milligram quantities of high-quality GPCRs and possibly other membrane proteins suitable for detailed characterization...
  49. pmc Crystal structure of native RPE65, the retinoid isomerase of the visual cycle
    Philip D Kiser
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Proc Natl Acad Sci U S A 106:17325-30. 2009
    ....
  50. pmc Retinopathy in mice induced by disrupted all-trans-retinal clearance
    Akiko Maeda
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 283:26684-93. 2008
    ..These findings provide direct evidence that aberrant production of toxic condensation byproducts of the visual cycle in mice can lead to rapid, progressive retinal degeneration...
  51. pmc Evolutionarily conserved long intergenic non-coding RNAs in the eye
    Debarshi Mustafi
    Department of Pharmacology, Cleveland Clinic, Cleveland, OH 44106 4965, USA
    Hum Mol Genet 22:2992-3002. 2013
    ..This combined approach identified several lincRNAs that could be critical for retinal and visual maintenance in adults. ..
  52. pmc Characterization of human β,β-carotene-15,15'-monooxygenase (BCMO1) as a soluble monomeric enzyme
    Thomas Kowatz
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Arch Biochem Biophys 539:214-22. 2013
    ..Establishment of a protocol that yields highly active homogenous BCMO1 is an important step towards clarifying the lipophilic substrate interaction, reaction mechanism and structure of this vitamin A forming enzyme...
  53. pmc Structural characterization of the rod cGMP phosphodiesterase 6
    Anna Goc
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4965, USA
    J Mol Biol 401:363-73. 2010
    ....
  54. doi request reprint QLT091001, a 9-cis-retinal analog, is well-tolerated by retinas of mice with impaired visual cycles
    Tadao Maeda
    Department of Ophthalmology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Invest Ophthalmol Vis Sci 54:455-66. 2013
    ....
  55. pmc Retinyl ester storage particles (retinosomes) from the retinal pigmented epithelium resemble lipid droplets in other tissues
    Tivadar Orban
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 286:17248-58. 2011
    ..These unexpected findings suggest a common mechanism for lipid droplet formation that exhibits broad chemical specificity for the hydrophobic substances being stored...
  56. pmc Retinyl ester homeostasis in the adipose differentiation-related protein-deficient retina
    Yoshikazu Imanishi
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 283:25091-102. 2008
    ..These observations suggest that Adfp plays a unique role in vision by maintaining proper storage and trafficking of retinoids within the eye...
  57. pmc Structural waters define a functional channel mediating activation of the GPCR, rhodopsin
    Thomas E Angel
    Department of Pharmacology, Center for Proteomics and Bioinformatics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Proc Natl Acad Sci U S A 106:14367-72. 2009
    ..These results suggest both a possible general mechanism for water-dependent communication in family A GPCRs based on structural conservation, and a strategy for probing membrane protein structure...
  58. pmc Inner retinal photoreception independent of the visual retinoid cycle
    Daniel C Tu
    Department of Ophthalmology and Visual Sciences, Washington University Medical School, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 103:10426-31. 2006
    ..These results demonstrate that the melanopsin-dependent ipRGC photocycle is independent of the visual retinoid cycle...
  59. pmc Insights into substrate specificity and metal activation of mammalian tetrahedral aspartyl aminopeptidase
    Yuanyuan Chen
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    J Biol Chem 287:13356-70. 2012
    ..This study provides a structural basis for understanding the physiology and bioinorganic chemistry of DNPEP and other M18 family aminopeptidases...
  60. pmc Efficient coupling of transducin to monomeric rhodopsin in a phospholipid bilayer
    Matthew R Whorton
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0632, USA
    J Biol Chem 283:4387-94. 2008
    ..These data suggest that monomeric rhodopsin is the minimal functional unit in G protein activation and that oligomerization is not absolutely required for this process...
  61. pmc Conformational changes in guanylate cyclase-activating protein 1 induced by Ca2+ and N-terminal fatty acid acylation
    Tivadar Orban
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Structure 18:116-26. 2010
    ..This model and the previously described "calcium-myristoyl switch" proposed for recoverin indicate significant diversity in conformational changes among these highly homologous NCS proteins with distinct functions...
  62. pmc Visualizing water molecules in transmembrane proteins using radiolytic labeling methods
    Tivadar Orban
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 49:827-34. 2010
    ..Although oil and water do not mix, understanding the roles of water is essential to understanding the function of membrane proteins...
  63. pmc Rhodopsin-transducin heteropentamer: three-dimensional structure and biochemical characterization
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    J Struct Biol 176:387-94. 2011
    ..The molecular envelope thus determined accommodated two Rho molecules together with one G(t) heterotrimer, corroborating the heteropentameric structure of the Rho(∗)·G(t) complex...
  64. pmc Increased adiposity in the retinol saturase-knockout mouse
    Alexander R Moise
    Department of Pharmacology, University of Kansas, Lawrence, KS, USA
    FASEB J 24:1261-70. 2010
    ..Moise, A. R., Lobo, G. P., Erokwu, B., Wilson, D. L., Peck, D., Alvarez, S., Domínguez, M., Alvarez, R., Flask, C. A., de Lera, A. R., von Lintig, J., Palczewski, K. Increased adiposity in the retinol saturase-knockout mouse...
  65. pmc Isolation and functional characterization of a stable complex between photoactivated rhodopsin and the G protein, transducin
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Wood Bldg, 10900 Euclid Ave, Cleveland, OH 44106 4965, USA
    FASEB J 23:371-81. 2009
    ..Moreover, in complexes regenerated with 9-cis-retinal/11-cis-retinal, Rho retains a conformation similar to Rho* that is stabilized by Gt(e) apo-protein...
  66. pmc Human cone photoreceptor dependence on RPE65 isomerase
    Samuel G Jacobson
    Scheie Eye Institute, Department of Ophthalmology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 104:15123-8. 2007
    ....
  67. pmc Role of membrane integrity on G protein-coupled receptors: Rhodopsin stability and function
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Prog Lipid Res 50:267-77. 2011
    ..Thus, altering the lipid organization of ROS membranes can result in visual dysfunction and blindness...
  68. pmc Phospholipids are needed for the proper formation, stability, and function of the photoactivated rhodopsin-transducin complex
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 48:5159-70. 2009
    ....
  69. pmc Structural basis of carotenoid cleavage: from bacteria to mammals
    Xuewu Sui
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 2109 Adelbert Rd, Cleveland, OH 44106 4965, USA
    Arch Biochem Biophys 539:203-13. 2013
    ..In this review, we aim to integrate structural and biochemical information about CCOs to provide insights into their catalytic mechanisms...
  70. pmc Sponge transgenic mouse model reveals important roles for the microRNA-183 (miR-183)/96/182 cluster in postmitotic photoreceptors of the retina
    Qubo Zhu
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 286:31749-60. 2011
    ..This study demonstrates a clear involvement of miRs in the physiology of postmitotic cells in vivo...
  71. pmc Substrate-induced changes in the dynamics of rhodopsin kinase (G protein-coupled receptor kinase 1)
    Tivadar Orban
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Biochemistry 51:3404-11. 2012
    ..In the presence of Mg(2+)·ATP, the influence of Rho* versus Rho on GRK1 dynamics was negligible...
  72. pmc A microparticle/hydrogel combination drug-delivery system for sustained release of retinoids
    Song Qi Gao
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Invest Ophthalmol Vis Sci 53:6314-23. 2012
    ....
  73. pmc Inflammatory priming predisposes mice to age-related retinal degeneration
    Debarshi Mustafi
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH, USA
    J Clin Invest 122:2989-3001. 2012
    ....
  74. pmc Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation
    Puja Singh
    Life Sciences Institute, Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    J Biol Chem 283:14053-62. 2008
    ..Co-localization of the N terminus and the C-terminal extension near the hinge of the kinase domain suggests that activated GPCRs stimulate kinase activity by binding to this region to facilitate full closure of the kinase domain...
  75. pmc Different properties of the native and reconstituted heterotrimeric G protein transducin
    Anna Goc
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 47:12409-19. 2008
    ..Therefore, Gt extracted and purified without subunit dissociation appears to be more appropriate for future studies...
  76. pmc Conserved waters mediate structural and functional activation of family A (rhodopsin-like) G protein-coupled receptors
    Thomas E Angel
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Proc Natl Acad Sci U S A 106:8555-60. 2009
    ....
  77. pmc Use of thallium to identify monovalent cation binding sites in GroEL
    Philip D Kiser
    Case Western Reserve University, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:967-71. 2009
    ..These findings also demonstrate the general usefulness of Tl(+) for the identification of monovalent cation-binding sites in protein crystal structures, even when the quality and resolution of the diffraction data are relatively low...
  78. pmc Posttranslational modifications of the photoreceptor-specific ABC transporter ABCA4
    Yaroslav Tsybovsky
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, United States
    Biochemistry 50:6855-66. 2011
    ..Thus, we present the first evidence that phosphorylation of ABCA4 can regulate its function...
  79. pmc Beta,beta-carotene decreases peroxisome proliferator receptor gamma activity and reduces lipid storage capacity of adipocytes in a beta,beta-carotene oxygenase 1-dependent manner
    Glenn P Lobo
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 285:27891-9. 2010
    ..Our study identifies beta,beta-carotene as a critical physiological precursor for RA production in adipocytes and implicates provitamin A as a dietary regulator of body fat reserves...
  80. pmc Improvement in rod and cone function in mouse model of Fundus albipunctatus after pharmacologic treatment with 9-cis-retinal
    Akiko Maeda
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Invest Ophthalmol Vis Sci 47:4540-6. 2006
    ..To assess changes in rod and cone visual functions in a mouse model of Fundus albipunctatus with disrupted 11-cis-retinol dehydrogenase (RDH) genes after pharmacologic treatment with an artificial retinal chromophore...
  81. pmc Heterogeneous N-terminal acylation of retinal proteins results from the retina's unusual lipid metabolism
    Grzegorz Bereta
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 50:3764-76. 2011
    ..Additionally, we propose a new hypothesis explaining the physiological relevance of elevated retinal ratios of C14:2- and C14:1-coenzyme A to C14:0-coenzyme A...
  82. pmc Crystal packing analysis of Rhodopsin crystals
    David T Lodowski
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH, USA
    J Struct Biol 158:455-62. 2007
    ..The inter-monomer surface contacts are different for each model, but several of these models coincide in implicating helix I, II, and H-8 as contributors to the main contact surface stabilizing the dimers...
  83. ncbi request reprint Heterologous expression and purification of the serotonin type 4 receptor from transgenic mouse retina
    David Salom
    Polgenix Inc, 11000 Cedar Avenue, Suite 260, Cleveland, Ohio 44106, USA
    Biochemistry 47:13296-307. 2008
    ..This expression method may prove generally useful for generating functional, high-quality GPCR protein...
  84. ncbi request reprint Effects of potent inhibitors of the retinoid cycle on visual function and photoreceptor protection from light damage in mice
    Akiko Maeda
    Department of Pharmacology, and Comprehensive Cancer Center, Case School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, BRB R924, Cleveland, OH 44106 4965, USA
    Mol Pharmacol 70:1220-9. 2006
    ....
  85. pmc Activation of G protein-coupled receptors: beyond two-state models and tertiary conformational changes
    Paul S H Park
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Annu Rev Pharmacol Toxicol 48:107-41. 2008
    ..More recent studies indicate a need to move beyond these classical paradigms and to consider additional aspects of the molecular character of GPCRs, such as the oligomerization and dynamics of the receptor...
  86. pmc Novel RDH12 mutations associated with Leber congenital amaurosis and cone-rod dystrophy: biochemical and clinical evaluations
    Wenyu Sun
    Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4965, USA
    Vision Res 47:2055-66. 2007
    ..Thus, impairment of retinal structure and function for patients carrying these mutations correlated with the biochemical properties of the mutants...
  87. pmc Conformational changes in the g protein-coupled receptor rhodopsin revealed by histidine hydrogen-deuterium exchange
    David T Lodowski
    Department of Pharmacology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106 4965, United States
    Biochemistry 49:9425-7. 2010
    ..These results indicate the utility of His-HDX in examining structural rearrangements in native source and membrane proteins without requiring structural modification...
  88. pmc Comparative analysis of GPCR crystal structures
    David T Lodowski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA
    Photochem Photobiol 85:425-30. 2009
    ..In this review we summarize recent progress on the structure and activation of rhodopsin in the context of other GPCR structures...
  89. pmc Usher syndrome IIIA gene clarin-1 is essential for hair cell function and associated neural activation
    Ruishuang Geng
    Department of Otolaryngology Head and Neck Surgery, Case Western Reserve University, Cleveland, OH 44106, USA
    Hum Mol Genet 18:2748-60. 2009
    ..In sum, hair cell dysfunction and prolonged peak latencies in vestibular and cochlear evoked potentials in Clrn1(-/-) mice strongly indicate that Clrn1 is necessary for hair cell function and associated neural activation...
  90. pmc The biochemical and structural basis for trans-to-cis isomerization of retinoids in the chemistry of vision
    Johannes von Lintig
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Trends Biochem Sci 35:400-10. 2010
    ..These 'ancestral footprints' in animal genomes bear witness to the common origin of the chemistry of vision, and will further stimulate research across evolutionary boundaries...
  91. pmc Dietary 9-cis-β,β-carotene fails to rescue vision in mouse models of leber congenital amaurosis
    Tadao Maeda
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44160, USA
    Mol Pharmacol 80:943-52. 2011
    ..Thus, our in vitro and in vivo studies revealed that 9-cis-BC is not a major source for mouse 9-cis-retinoid production but is mainly converted to all-trans-retinoids to support canonical vitamin A action...
  92. pmc An acyl-covalent enzyme intermediate of lecithin:retinol acyltransferase
    Marcin Golczak
    Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 285:29217-22. 2010
    ..These observations are critical to understanding the catalytic mechanism of LRAT protein family members as well as other lecithin:acyltransferases wherein Cys residues are required for catalysis...
  93. ncbi request reprint Topology and membrane association of lecithin: retinol acyltransferase
    Alexander R Moise
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    J Biol Chem 282:2081-90. 2007
    ..We present experimental evidence of the topology and subcellular localization of LRAT, a critical enzyme in vitamin A metabolism...
  94. pmc Stabilizing effect of Zn2+ in native bovine rhodopsin
    Paul S H Park
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 282:11377-85. 2007
    ..The inclusion of ZnCl2 in SMFS assay buffer increased the stability of most structural segments. This effect was not mimicked by CaCl2, CdCl2, or CoCl2. Thus, Zn2+ stabilizes the structure of rhodopsin in a specific manner...
  95. pmc Delivery of retinoid-based therapies to target tissues
    Alexander R Moise
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 46:4449-58. 2007
    ..Future retinoid-based therapeutic strategies could involve targeted delivery mechanisms leading to lower toxicity and improved effectiveness of retinoids...
  96. pmc Specificity of zebrafish retinol saturase: formation of all-trans-13,14-dihydroretinol and all-trans-7,8- dihydroretinol
    Alexander R Moise
    Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Biochemistry 46:1811-20. 2007
    ..These findings point to a conserved function but altered specificity of RetSat in vertebrates, leading to the generation of various dihydroretinoid compounds, some of which could have signaling functions...
  97. pmc Complexes between photoactivated rhodopsin and transducin: progress and questions
    Beata Jastrzebska
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Biochem J 428:1-10. 2010
    ..Efficient and reproducible formation of physiologically relevant, conformationally homogenous GPCR-G-protein complexes is a prerequisite for structural studies designed to address these possibilities...
  98. pmc The ATP-binding cassette transporter ABCA4: structural and functional properties and role in retinal disease
    Yaroslav Tsybovsky
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH, USA
    Adv Exp Med Biol 703:105-25. 2010
    ..The pathogenesis of diseases caused by mutations in ABCA4 is complex, comprising a loss-of-function component as well as photoreceptor stress caused by protein mislocalization and misfolding...
  99. pmc Functional and structural characterization of rhodopsin oligomers
    Beata Jastrzebska
    Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 281:11917-22. 2006
    ..Our studies provide direct comparisons between signaling properties of Meta II in different quaternary complexes...
  100. pmc Chemokine receptors and other G protein-coupled receptors
    David T Lodowski
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Curr Opin HIV AIDS 4:88-95. 2009
    ..CXCR4 and CCR5 are utilized as co-receptors during HIV viral entry and, therefore, crystal structures of GPCRs aid in the understanding of their function in viral entry...
  101. pmc Topology of class A G protein-coupled receptors: insights gained from crystal structures of rhodopsins, adrenergic and adenosine receptors
    Debarshi Mustafi
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Mol Pharmacol 75:1-12. 2009
    ....

Research Grants51

  1. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2003
    ....
  2. STRUCTURAL STUDIES OF ARRESTINS
    Krzysztof Palczewski; Fiscal Year: 2000
    ....
  3. RETINOIDS IN VISION
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2010
    ..Studies proposed in this application will add new biochemical and physiological information to steps in the retinoid cycle that are as yet poorly understood but which are critical for the normal function of photoreceptors. ..
  4. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2005
    ....
  5. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2005
    ..This is a rare example in biology where basic understanding of fundamental processes at the structural level feeds back for potential pharmacological intervention with small chaperone-like molecules to refold mutated protein. ..
  6. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2005
    ....
  7. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2002
    ....
  8. PHOTOTRANSDUCTION ENZYMES
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2010
    ..Therefore, the long-term objective of our research is to elucidate the detailed molecular events involved in phototransduction. ..
  9. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2009
    ..Therefore, the long-term objective of our research is to elucidate the detailed molecular events involved in phototransduction. ..
  10. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2006
    ....
  11. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2007
    ..This is a rare example in biology where basic understanding of fundamental processes at the structural level feeds back for potential pharmacological intervention with small chaperone-like molecules to refold mutated protein. ..
  12. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2009
    ..Studies proposed in this application will add new biochemical and physiological information to steps in the retinoid cycle that are as yet poorly understood but which are critical for the normal function of photoreceptors. ..
  13. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2007
    ..abstract_text> ..
  14. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2005
    ..This is a rare example in biology where basic understanding of fundamental processes at the structural level feeds back for potential pharmacological intervention with small chaperone-like molecules to refold mutated protein. ..
  15. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2006
    ..This is a rare example in biology where basic understanding of fundamental processes at the structural level feeds back for potential pharmacological intervention with small chaperone-like molecules to refold mutated protein. ..
  16. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2007
    ..abstract_text> ..
  17. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2003
    ....
  18. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 1999
    ....
  19. STRUCTURAL STUDIES OF ARRESTINS
    Krzysztof Palczewski; Fiscal Year: 1999
    ....
  20. STRUCTURAL STUDIES OF ARRESTINS
    Krzysztof Palczewski; Fiscal Year: 2001
    ....
  21. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2003
    ....
  22. IMAGING OF ROD OUTER SEGMENT BY CRYO-ELECTRON TOMOGRAPHY
    Krzysztof Palczewski; Fiscal Year: 2007
    ..These studies will add significantly to our understanding of the normal and disease stage organization of the rod outer segments. ..
  23. STRUCTURAL STUDIES OF G PROTEIN-COUPLED RECEPTORS
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2010
    ..The importance of the protein family for human health makes this effort worthwhile. ..
  24. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2007
    ..Studies proposed in this application will add new biochemical and physiological information to steps in the retinoid cycle that are as yet poorly understood but which are critical for the normal function of photoreceptors. ..
  25. STRUCTURAL STUDIES OF ARRESTIN AND RHODOPSIN PHOSPHATASE
    Krzysztof Palczewski; Fiscal Year: 1992
    ..4) to characterize beta-arrestin, a homolog of arrestin, including its functional and immunological properties. (5) to definitively identify the rhodopsin phosphatase and an inhibitor of its activity...
  26. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2001
    ....
  27. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2004
    ..This is a rare example in biology where basic understanding of fundamental processes at the structural level feeds back for potential pharmacological intervention with small chaperone-like molecules to refold mutated protein. ..
  28. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2004
    ....
  29. RETINOIDS IN VISION
    Krzysztof Palczewski; Fiscal Year: 2009
    ..Studies proposed in this application will add new biochemical and physiological information to steps in the retinoid cycle that are as yet poorly understood but which are critical for the normal function of photoreceptors. ..
  30. STRUCTURAL STUDIES OF G PROTEIN-COUPLED RECEPTORS
    Krzysztof Palczewski; Fiscal Year: 2009
    ..The importance of the protein family for human health makes this effort worthwhile. ..
  31. STRUCTURAL STUDIES OF ARRESTIN AND RHODOPSIN PHOSPHATASE
    Krzysztof Palczewski; Fiscal Year: 1993
    ..4) to characterize beta-arrestin, a homolog of arrestin, including its functional and immunological properties. (5) to definitively identify the rhodopsin phosphatase and an inhibitor of its activity...
  32. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2000
    ....
  33. STRUCTURAL STUDIES OF G PROTEIN-COUPLED RECEPTORS
    Krzysztof Palczewski; Fiscal Year: 2007
    ..The importance of the protein family for human health makes this effort worthwhile. ..
  34. STRUCTURAL STUDIES OF G PROTEIN-COUPLED RECEPTORS
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2010
    ..The importance of the protein family for human health makes this effort worthwhile. ..
  35. VERTEBRATE CAROTENOID ISOMERASES
    Krzysztof Palczewski; Fiscal Year: 2005
    ..Lastly, the contribution of these enzymes to retinoid processing and, hence, to vision will be studied using transgenic animal models. ..
  36. PHOTOTRANSDUCTION ENZYMES
    Krzysztof Palczewski; Fiscal Year: 2002
    ....