Research Topics
Genomes and Genes
| Laura E NagySummaryAffiliation: Case Western Reserve University Country: USA Publications
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Detail Information
Publications
Molecular aspects of alcohol metabolism: transcription factors involved in early ethanol-induced liver injuryLaura E Nagy
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
Annu Rev Nutr 24:55-78. 2004....
Stabilization of tumor necrosis factor-alpha mRNA in macrophages in response to chronic ethanol exposureLaura E Nagy
Department of Nutrition, Case Western Reserve University, 2123 Abington Road, Room 201, Cleveland, OH 44106 4906, USA
Alcohol 33:229-33. 2004..Taken together, the results from these studies identify the regulation of TNF-alpha mRNA stability as a novel mechanism by which chronic exposure to ethanol increases the expression of TNF-alpha...
Ethanol and membrane protein trafficking: diverse mechanisms of ethanol actionLaura E Nagy
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
Alcohol Clin Exp Res 26:287-93. 2002..Casey; (3) Ethanol and L1 mediated neurite outgrowth: receptor recycling and L1 signal transduction, by Cynthia F. Bearer; and (4) Ethanol and glucose transport, GLUT4 vesicle trafficking, by Laura E. Nagy...
Recent insights into the role of the innate immune system in the development of alcoholic liver diseaseLaura E Nagy
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
Exp Biol Med (Maywood) 228:882-90. 2003..Enhanced activation of these TLR-4 dependent signaling pathways after chronic ethanol likely contributes to the development of alcoholic liver disease...
Complement and alcoholic liver disease: role of C1q in the pathogenesis of ethanol-induced liver injury in miceJessica I Cohen
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44195, USA
Gastroenterology 139:664-74, 674.e1. 2010..Because ethanol exposure increases hepatocellular apoptosis, we hypothesized that ethanol-induced apoptosis would lead to activation of complement via the classical pathway...
Short-term treatment of RAW264.7 macrophages with adiponectin increases tumor necrosis factor-alpha (TNF-alpha) expression via ERK1/2 activation and Egr-1 expression: role of TNF-alpha in adiponectin-stimulated interleukin-10 productionPil Hoon Park
Department of Pathobiology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
J Biol Chem 282:21695-703. 2007....
Chronic ethanol and triglyceride turnover in white adipose tissue in rats: inhibition of the anti-lipolytic action of insulin after chronic ethanol contributes to increased triglyceride degradationLi Kang
Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106, USA
J Biol Chem 282:28465-73. 2007..Furthermore, this enhanced rate of lipolysis was due to a suppression of the anti-lipolytic effects of insulin in adipocytes after chronic ethanol feeding...
Chronic ethanol feeding increases activation of NADPH oxidase by lipopolysaccharide in rat Kupffer cells: role of increased reactive oxygen in LPS-stimulated ERK1/2 activation and TNF-alpha productionVarsha Thakur
Department of Nutrition, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-4906, USA
J Leukoc Biol 79:1348-56. 2006..Further, ERK1/2 is an important target of NADPH oxidase-derived ROS in Kupffer cells, contributing to enhanced LPS-stimulated TNF-alpha production by Kupffer cells after chronic ethanol feeding...
Stimulation of macrophage TNFalpha production by orthopaedic wear particles requires activation of the ERK1/2/Egr-1 and NF-kappaB pathways but is independent of p38 and JNKMichelle A Beidelschies
Department of Physiology and Biophysics, Case Medical Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
J Cell Physiol 217:652-66. 2008....
Lipopolysaccharide stimulation of ERK1/2 increases TNF-alpha production via Egr-1Liang Shi
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
Am J Physiol Cell Physiol 282:C1205-11. 2002..These results delineate the role of ERK1/2 activation of Egr-1 activity in mediating LPS-induced increases in TNF-alpha mRNA expression in macrophages...
Chronic ethanol-induced insulin resistance is associated with macrophage infiltration into adipose tissue and altered expression of adipocytokinesLi Kang
Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio, USA
Alcohol Clin Exp Res 31:1581-8. 2007....
An early complement-dependent and TLR-4-independent phase in the pathogenesis of ethanol-induced liver injury in miceSanjoy Roychowdhury
Department of Pathobiology, Cleveland Clinic Foundation, Cleveland, OH, USA
Hepatology 49:1326-34. 2009..CONCLUSION: We have identified a complement- and macrophage-dependent, but TLR-4 independent, phase in the pathogenesis of ethanol-induced liver injury...
Ethanol-induced oxidative stress via the CYP2E1 pathway disrupts adiponectin secretion from adipocytesHui Tang
Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA
Alcohol Clin Exp Res 36:214-22. 2012..Here, we investigated the role of cytochrome P450 2E1 (CYP2E1) and oxidative stress in the mechanism for impaired adiponectin secretion from adipocytes in response to ethanol...
Regulation of macrophage activation in alcoholic liver diseaseVarsha Thakur
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
J Gastroenterol Hepatol 22:S53-6. 2007....
Chronic alcohol exposure increases circulating bioactive oxidized phospholipidsLili Yang
Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio 44195, USA
J Biol Chem 285:22211-20. 2010..Suppressing hepatic inflammation, not ethanol catabolism, reduces circulating inflammatory and apoptotic agonists...
Formation of gamma-ketoaldehyde-protein adducts during ethanol-induced liver injury in miceSanjoy Roychowdhury
Department of Pathobiology, Cleveland Clinic, Cleveland, OH 44195, USA
Free Radic Biol Med 47:1526-38. 2009..In summary, ethanol feeding enhanced gamma-ketoaldehyde-protein adduct production via a TNFR1/CYP2E1-dependent, but cyclooxygenase-independent, mechanism in mouse liver...
Methods to investigate the effects of chronic ethanol on adipocytesBecky M Sebastian
Department of Pathobiology, Cleveland Clinic, Cleveland, OH, USA
Methods Mol Biol 447:357-66. 2008..Included in this chapter are protocols for studying the effect of chronic ethanol feeding on these adipocyte functions...
Chronic ethanol exposure increases the binding of HuR to the TNFalpha 3'-untranslated region in macrophagesMegan R McMullen
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
J Biol Chem 278:38333-41. 2003..Taken together, these data demonstrate that increased binding of HuR to the TNFalpha 3'-UTR contributes to increased LPS-stimulated TNFalpha expression in macrophages after chronic ethanol exposure...
Molecular mechanism for adiponectin-dependent M2 macrophage polarization: link between the metabolic and innate immune activity of full-length adiponectinPalash Mandal
Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Biol Chem 286:13460-9. 2011....
Differential contributions of C3, C5, and decay-accelerating factor to ethanol-induced fatty liver in miceMichele T Pritchard
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44905, USA
Gastroenterology 132:1117-26. 2007..The complement pathway is an important component of the innate and adaptive immune response. Here we tested the hypothesis that activation of complement is required for development of ethanol-induced fatty liver...
Anti-inflammatory pathways and alcoholic liver disease: role of an adiponectin/interleukin-10/heme oxygenase-1 pathwayPalash Mandal
Department of Pathobiology and Gastroenterology, Cleveland Clinic, Lerner Research Institute NE 40, 9500 Euclid Avenue, Cleveland, OH 44195, USA
World J Gastroenterol 16:1330-6. 2010....
Suppression of lipopolysaccharide-stimulated tumor necrosis factor-alpha production by adiponectin is mediated by transcriptional and post-transcriptional mechanismsPil Hoon Park
Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Biol Chem 283:26850-8. 2008..gAcrp treatment profoundly suppressed the ability of LPS to increase TNF-alpha transcription and reduced the stimulus-induced stabilization of TNF-alpha mRNA in response to LPS...
Adiponectin and heme oxygenase-1 suppress TLR4/MyD88-independent signaling in rat Kupffer cells and in mice after chronic ethanol exposurePalash Mandal
Department of Pathobiology, Cleveland Clinic, Cleveland, OH 44195, USA
J Immunol 185:4928-37. 2010..These data suggest that induction of HO-1 may be a useful therapeutic strategy in alcoholic liver disease...
Adiponectin normalizes LPS-stimulated TNF-alpha production by rat Kupffer cells after chronic ethanol feedingVarsha Thakur
Department of Nutrition, Case Western Reserve University, Rm 201, 2123 Abington Road, Cleveland, OH 44106 4906, USA
Am J Physiol Gastrointest Liver Physiol 290:G998-1007. 2006..Suppression of LPS-stimulated ERK1/2 signaling by low concentrations of gAcrp was associated with normalization of TNF-alpha production by Kupffer cells after chronic ethanol exposure...
Endothelin-1-stimulated glucose uptake is desensitized by tumor necrosis factor-alpha in 3T3-L1 adipocytesNadia Rachdaoui
Department of Nutrition, Case Western Reserve University, Cleveland, OH 44106, USA
Am J Physiol Endocrinol Metab 285:E545-51. 2003....
Isolation of Kupffer cells from rats fed chronic ethanolMegan R McMullen
Department Pathobiology, Cleveland Clinic, Cleveland, OH, USA
Methods Mol Biol 447:199-212. 2008..The isolation and culture of Kupffer cells is an important technique with which one can elucidate the mechanisms that contribute to alcoholic liver injury...
Taurine supplementation prevents ethanol-induced decrease in serum adiponectin and reduces hepatic steatosis in ratsXiaocong Chen
Department of Nutrition, Case Western Reserve University, Cleveland, OH, USA
Hepatology 49:1554-62. 2009..Taken together, these data demonstrate that taurine has important protective effects against ethanol-induced tissue injury in both adipose and liver tissue...
Regulation of Kupffer cell activity during chronic ethanol exposure: role of adiponectinPil-Hoon Park
Department of Nutrition, Case Western Reserve University, Cleveland and Departments of Gastroenterology and Pathobiology, Cleveland Clinic Foundation, Cleveland, Ohio 44106-4906, USA
J Gastroenterol Hepatol 21:S30-3. 2006..These studies suggest that the hepato-protective activity of adiponectin is due, at least in part, to a direct anti-inflammatory effect of adiponectin on Kupffer cells...
Early growth response-1 transcription factor is essential for ethanol-induced fatty liver injury in miceMegan R McMullen
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
Gastroenterology 128:2066-76. 2005..Furthermore, because TNF-alpha contributes to alcoholic liver injury, we tested the hypothesis that Egr-1 is required for the development of ethanol-induced fatty liver injury in wild type and egr-1 -/- mice...
Decreased insulin-dependent glucose transport by chronic ethanol feeding is associated with dysregulation of the Cbl/TC10 pathway in rat adipocytesBecky M Sebastian
Dept of Nutrition, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4906, USA
Am J Physiol Endocrinol Metab 289:E1077-84. 2005....
Identification of a cytochrome P4502E1/Bid/C1q-dependent axis mediating inflammation in adipose tissue after chronic ethanol feeding to miceBecky M Sebastian
Liver Disease Research Center, Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Biol Chem 286:35989-97. 2011..Taken together, these data identify a novel mechanism for the development of adipose tissue inflammation that likely contributes to the pathophysiological effects of ethanol...
Exogenous thioredoxin prevents ethanol-induced oxidative damage and apoptosis in mouse liverJessica I Cohen
Department of Nutrition, Case Western Reserve University, Cleveland, OH, USA
Hepatology 49:1709-17. 2009..rhTrx treatment prevented these increases. In summary, rhTrx attenuated ethanol-induced increases in markers of oxidative stress, inflammatory cytokine expression, and apoptosis...
Mechanisms for the anti-inflammatory effects of adiponectin in macrophagesHonglian Huang
Department of Pathobiology, Cleveland Clinic, Cleveland, OH 44195, USA
J Gastroenterol Hepatol 23:S50-3. 2008..Increased IL-10 expression was ultimately required for the suppression of TLR4-mediated signaling by gAcrp...
Chronic ethanol increases lipopolysaccharide-stimulated Egr-1 expression in RAW 264.7 macrophages: contribution to enhanced tumor necrosis factor alpha productionLiang Shi
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
J Biol Chem 277:14777-85. 2002..These results demonstrate that enhanced LPS-dependent activation of Egr-1 contributes to increased TNFalpha production after chronic ethanol exposure...
Isolevuglandins covalently modify phosphatidylethanolamines in vivo: detection and quantitative analysis of hydroxylactam adductsWei Li
Department of Chemistry, Case Western Reserve University, Cleveland, OH 44106, USA
Free Radic Biol Med 47:1539-52. 2009..4+/-0.1 ng/ml, n=15). Just as LG/isoLG-protein adducts provide a dosimeter of oxidative injury, this study suggests that LG/isoLG-PE-hydroxylactams are potential biomarkers for assessing risk for oxidative stress-stimulated diseases...
Role of complement in ethanol-induced liver injuryMichele T Pritchard
Department of Pathobiology, Cleveland Clinic, Cleveland, OH 44195, USA
Adv Exp Med Biol 632:175-86. 2008....
Early growth response-1 contributes to galactosamine/lipopolysaccharide-induced acute liver injury in miceMichele T Pritchard
Dept of Pathobiology, Cleveland Clinic, 9500 Euclid Ave NE40, Cleveland, OH 44195, USA
Am J Physiol Gastrointest Liver Physiol 293:G1124-33. 2007..These data demonstrate that Egr-1 plays an important role in acceleration of hepatic inflammation, apoptosis, and subsequent mortality in GalN/LPS-induced acute liver injury...
Chronic ethanol feeding impairs endothelin-1-stimulated glucose uptake via decreased G alpha 11 expression in rat adipocytesNadia Rachdaoui
Dept of Nutrition, Case Western Reserve University, 2123 Abington Rd, Rm 201, Cleveland, OH 44106 4906, USA
Am J Physiol Endocrinol Metab 285:E303-10. 2003....
Chronic ethanol feeding suppresses beta-adrenergic receptor-stimulated lipolysis in adipocytes isolated from epididymal fatLi Kang
Department of Nutrition, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106-4906, USA
Endocrinology 147:4330-8. 2006....
Inhibition of apoptosis protects mice from ethanol-mediated acceleration of early markers of CCl(4) -induced fibrosis but not steatosis or inflammationSanjoy Roychowdhury
Center for Liver Disease Research, Departments of Pathobiology, Case Western Reserve University, Cleveland, Ohio, USA
Alcohol Clin Exp Res 36:1139-47. 2012....
Early growth response-1 attenuates liver injury and promotes hepatoprotection after carbon tetrachloride exposure in miceMichele T Pritchard
Department of Pathobiology, Cleveland Clinic, Cleveland, OH 44195, USA
J Hepatol 53:655-62. 2010..Here, we tested the hypothesis that Egr-1 is beneficial after toxic liver injury...
Chronic ethanol feeding to rats decreases adiponectin secretion by subcutaneous adipocytesXiaocong Chen
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio, USA
Am J Physiol Endocrinol Metab 292:E621-8. 2007..In conclusion, chronic ethanol feeding impaired adiponectin secretion by subcutaneous and retroperitoneal adipocytes; impaired secretion likely contributes to decreased adiponectin concentrations after chronic ethanol feeding...
Hepatic fibrosis is enhanced and accompanied by robust oval cell activation after chronic carbon tetrachloride administration to Egr-1-deficient miceMichele T Pritchard
Department of Pathobiology NE40, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
Am J Pathol 176:2743-52. 2010..Taken together, these data reveal novel roles for Egr-1 as a negative regulator of both CCl(4)-induced hepatic fibrosis and the oval cell response...
Early growth response (EGR)-1 is required for timely cell-cycle entry and progression in hepatocytes after acute carbon tetrachloride exposure in miceMichele T Pritchard
Department of Pathobiology, Cleveland Clinic, Case Western Reserve University, Ohio 44106, USA
Am J Physiol Gastrointest Liver Physiol 300:G1124-31. 2011..These data support the hypothesis that Egr-1 provides hepatoprotection in the CCl(4)-injured liver, attributable, in part, to timely cell-cycle induction and progression...
ERK1/2 and Egr-1 contribute to increased TNF-alpha production in rat Kupffer cells after chronic ethanol feedingRaj Kishore
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106 4906, USA
Am J Physiol Gastrointest Liver Physiol 282:G6-15. 2002..Together, these data suggest that enhanced activation of ERK1/2 and Egr-1 contributes to increased TNF-alpha production after chronic ethanol feeding...
Pathogenesis of alcoholic liver disease: interactions between parenchymal and non-parenchymal cellsJessica I Cohen
Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Dig Dis 12:3-9. 2011..In this review we provide an overview of the complex interactions between parenchymal and non-parenchymal cells in the liver during the progression of ethanol-induced liver injury...
Redox signaling and the innate immune system in alcoholic liver diseaseJessica I Cohen
Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
Antioxid Redox Signal 15:523-34. 2011..These data illustrate that ethanol-induced oxidative stress and activation of the innate immune system interact dynamically during ethanol exposure, exacerbating ethanol-induced liver injury...
Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitisLaura J Dixon
Department of Molecular Medicine, Case Western Reserve University, Cleveland, Ohio, United States of America
PLoS ONE 8:e56100. 2013..Specifically, mice deficient in caspase-1 are protected from high fat-induced hepatic steatosis, inflammation and early fibrogenesis. These data point to the inflammasome as an important therapeutic target for NASH...
Nutritional control of mRNA stability is mediated by a conserved AU-rich element that binds the cytoplasmic shuttling protein HuRIbrahim Yaman
Departments of Nutrition and Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio, 44106-4906, USA
J Biol Chem 277:41539-46. 2002..It is proposed that an adaptive response of cells to nutritional stress results in increased mRNA stability mediated by HuR binding to the NS-ARE...
Activation of cyclic-AMP response element binding protein contributes to adiponectin-stimulated interleukin-10 expression in RAW 264.7 macrophagesPil Hoon Park
Department of Pathobiology, Cleveland Clinic, OH 44195, USA
J Leukoc Biol 83:1258-66. 2008..Taken together, these data identify gAcrp-stimulated phospho-CREB as a key transcription factor responsible for gAcrp-induced IL-10 promoter activation...
Acute and chronic ethanol exposure. PrefaceLaura E Nagy
Cleveland Clinic, USA
Methods Mol Biol 447:v-vi. 2008
Acute and chronic alcohol abuse modulate immunityLou Ann S Brown
Department of Pediatrics, Emory University, Atlanta, Georgia, USA
Alcohol Clin Exp Res 30:1624-31. 2006..Nagy, and (8) Hepatitis C Virus Infection and Alcohol Use by Gyongyi Szabo. Meeting coorganizers were Elizabeth J. Kovacs, Lou Ann S. Brown, Thomas R. Jerrells, and Robert T. Cook...
