Eckhard Jankowsky

Summary

Affiliation: Case Western Reserve University
Country: USA

Publications

  1. pmc SF1 and SF2 helicases: family matters
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Curr Opin Struct Biol 20:313-24. 2010
  2. pmc The RNA helicase database
    Anja Jankowsky
    Department of Biochemistry, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Nucleic Acids Res 39:D338-41. 2011
  3. pmc RNA helicases at work: binding and rearranging
    Eckhard Jankowsky
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Trends Biochem Sci 36:19-29. 2011
  4. pmc Remodeling of ribonucleoprotein complexes with DExH/D RNA helicases
    Eckhard Jankowsky
    Department of Biochemistry, Center for RNA Molecular Biology, Wood W447, School of Medicine Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44122, USA
    Nucleic Acids Res 34:4181-8. 2006
  5. pmc Discriminatory RNP remodeling by the DEAD-box protein DED1
    Heath A Bowers
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    RNA 12:903-12. 2006
  6. ncbi request reprint DEAD-box proteins unwind duplexes by local strand separation
    Quansheng Yang
    Department of Biochemistry, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Mol Cell 28:253-63. 2007
  7. pmc RNA unwinding by the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex
    Huijue Jia
    Center for RNA Molecular Biology, Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 109:7292-7. 2012
  8. pmc Unwinding initiation by the viral RNA helicase NPH-II
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    J Mol Biol 415:819-32. 2012
  9. ncbi request reprint The DEAD-box protein Ded1 unwinds RNA duplexes by a mode distinct from translocating helicases
    Quansheng Yang
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, Ohio 44106, USA
    Nat Struct Mol Biol 13:981-6. 2006
  10. pmc AMP sensing by DEAD-box RNA helicases
    Andrea A Putnam
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    J Mol Biol 425:3839-45. 2013

Collaborators

  • Timothy W Nilsen
  • Reinhard Luhrmann
  • James T Anderson
  • Fei Liu
  • Vernon E Anderson
  • Michael E Harris
  • Wen Wang
  • Paul D Gollnick
  • Ulf Peter Guenther
  • Quansheng Yang
  • Andrea A Putnam
  • Huijue Jia
  • Margaret E Fairman-Williams
  • Andrea Putnam
  • Margaret E Fairman
  • Patricia A Maroney
  • Mridusmita Saikia
  • Heath A Bowers
  • Varandt Khodaverdian
  • Xuying Wang
  • Anja Jankowsky
  • Yang Yu
  • Zhaoyang Feng
  • Guo fu Hu
  • Tao Pan
  • Maria Hatzoglou
  • Marc Parisien
  • Bo Jhih Guan
  • Raul Jobava
  • Marianne Pusztai-Carey
  • Naresh Babu V Sepuri
  • Yiyuan Yuan
  • Xing Huang Gao
  • Madhavi Gorla
  • Dawid Krokowski
  • Barbara Truitt
  • Michelle Pesho
  • Stanley Nithianantham
  • Guanping Yu
  • Menachem Shoham
  • Frank E Campbell
  • Elizabeth Delaney
  • David Anderson
  • Lindsay E Yandek
  • Courtney N Niland
  • Parita Patel
  • Lucy Bollinger
  • Sukanya Srinivasan
  • Xiang H F Zhang
  • John A Denker
  • Olexandr Dybkov
  • Lawrence A Chasin
  • Mark Del Campo
  • Alan M Lambowitz
  • Berthold Kastner

Detail Information

Publications26

  1. pmc SF1 and SF2 helicases: family matters
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Curr Opin Struct Biol 20:313-24. 2010
    ..We discuss recent data in the context of this unifying view of SF1 and SF2 helicases...
  2. pmc The RNA helicase database
    Anja Jankowsky
    Department of Biochemistry, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Nucleic Acids Res 39:D338-41. 2011
    ..Information on individual RNA helicases can be accessed through various browsing routes or through text-based searches of the database...
  3. pmc RNA helicases at work: binding and rearranging
    Eckhard Jankowsky
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Trends Biochem Sci 36:19-29. 2011
    ..This review outlines our current view on major structural and mechanistic themes of RNA helicase function, and on emerging physical models for cellular roles of these enzymes...
  4. pmc Remodeling of ribonucleoprotein complexes with DExH/D RNA helicases
    Eckhard Jankowsky
    Department of Biochemistry, Center for RNA Molecular Biology, Wood W447, School of Medicine Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44122, USA
    Nucleic Acids Res 34:4181-8. 2006
    ....
  5. pmc Discriminatory RNP remodeling by the DEAD-box protein DED1
    Heath A Bowers
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    RNA 12:903-12. 2006
    ..In addition, our study illuminates possible mechanisms for protein displacement by DExH/D proteins...
  6. ncbi request reprint DEAD-box proteins unwind duplexes by local strand separation
    Quansheng Yang
    Department of Biochemistry, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Mol Cell 28:253-63. 2007
    ..We propose a three-step mechanism in which DEAD-box proteins unwind duplexes as "local strand separators." This unwinding mode is well-suited for local structural changes in complex RNA or RNP assemblies...
  7. pmc RNA unwinding by the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex
    Huijue Jia
    Center for RNA Molecular Biology, Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 109:7292-7. 2012
    ..Our results suggest a model for coordination between unwinding and polyadenylation activities by TRAMP that reveals remarkable synergy between helicase and poly(A) polymerase...
  8. pmc Unwinding initiation by the viral RNA helicase NPH-II
    Margaret E Fairman-Williams
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    J Mol Biol 415:819-32. 2012
    ....
  9. ncbi request reprint The DEAD-box protein Ded1 unwinds RNA duplexes by a mode distinct from translocating helicases
    Quansheng Yang
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave, Cleveland, Ohio 44106, USA
    Nat Struct Mol Biol 13:981-6. 2006
    ..This new type of helicase activity explains observations with other DEAD-box proteins and may be the prototype for duplex-unwinding reactions in RNA metabolism...
  10. pmc AMP sensing by DEAD-box RNA helicases
    Andrea A Putnam
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    J Mol Biol 425:3839-45. 2013
    ..However, the yeast DEAD-box helicases Sub2p and Dbp5p are not inhibited by AMP. Our observations identify a subset of DEAD-box helicases as enzymes with the capacity to directly link changes in AMP concentrations to RNA metabolism. ..
  11. pmc Hidden specificity in an apparently nonspecific RNA-binding protein
    Ulf Peter Guenther
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Nature 502:385-8. 2013
    ..Our findings suggest that apparently nonspecific and specific RNA-binding modes may not differ fundamentally, but represent distinct parts of common affinity distributions. ..
  12. doi request reprint Helicase multitasking in ribosome assembly
    Ulf Peter Guenther
    Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA
    Mol Cell 36:537-8. 2009
    ..In this issue of Molecular Cell, Bohnsack et al. (2009) identify multiple binding sites of the RNA helicase Prp43 on preribosomal RNA. The target regions suggest distinct functions of Prp43 in ribosome biogenesis...
  13. pmc ATP hydrolysis is required for DEAD-box protein recycling but not for duplex unwinding
    Fei Liu
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 105:20209-14. 2008
    ..ATP binding, however, appears necessary. ATP hydrolysis is found to be required for fast enzyme release from the RNA and multiple substrate turnovers and thus for enzyme recycling...
  14. doi request reprint Duplex unwinding and RNP remodeling with RNA helicases
    Eckhard Jankowsky
    Department of Biochemistry, Case Western Reserve University, Ohio, Cleveland, USA
    Methods Mol Biol 488:343-55. 2008
    ..We show how to prepare substrates for these reactions and how to monitor unwinding of RNA duplexes and displacement of proteins from RNA using standard molecular biology techniques...
  15. ncbi request reprint RNA helicases: versatile ATP-driven nanomotors
    Eckhard Jankowsky
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    J Nanosci Nanotechnol 5:1983-9. 2005
    ..In this review, we discuss the structure and function of RNA helicases with an emphasis on the potential application of these enzymes to control conformational changes in nanoassemblies that contain RNA...
  16. pmc DEAD-box-protein-assisted RNA structure conversion towards and against thermodynamic equilibrium values
    Quansheng Yang
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    J Mol Biol 368:1087-100. 2007
    ..Our results provide a basic mechanistic framework for protein-assisted RNA structure conversions that illuminates the role of ATP hydrolysis and reveal an unexpected diversity of pathways...
  17. ncbi request reprint ATP- and ADP-dependent modulation of RNA unwinding and strand annealing activities by the DEAD-box protein DED1
    Quansheng Yang
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Biochemistry 44:13591-601. 2005
    ....
  18. pmc The RNA helicase Mtr4p modulates polyadenylation in the TRAMP complex
    Huijue Jia
    Center for RNA Molecular Biology and Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Cell 145:890-901. 2011
    ..Our data establish Mtr4p as a critical regulator of polyadenylation by TRAMP and reveal that an RNA helicase can control the activity of another enzyme in a highly complex fashion and in response to features in RNA...
  19. pmc Dynamic regulation of alternative splicing by silencers that modulate 5' splice site competition
    Yang Yu
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106, USA
    Cell 135:1224-36. 2008
    ..The data indicate that perturbations of non-rate-limiting step(s) in splicing can lead to dramatic shifts in splice site choice...
  20. pmc DEAD-box helicases as integrators of RNA, nucleotide and protein binding
    Andrea A Putnam
    Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA
    Biochim Biophys Acta 1829:884-93. 2013
    ..We discuss these data in the context of a model that views the enzymes as integrators of RNA, nucleotide, and protein binding. This article is part of a Special Issue entitled: The Biology of RNA helicases - Modulation for life...
  21. doi request reprint Analysis of duplex unwinding by RNA helicases using stopped-flow fluorescence spectroscopy
    Andrea Putnam
    Department of Biochemistry, Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    Methods Enzymol 511:1-27. 2012
    ..We discuss advantages, disadvantages, and technical considerations for stopped-flow approaches, as well as substrate design. In addition, we list protocols and explain functional information obtained with these experiments...
  22. ncbi request reprint Protein displacement by DExH/D "RNA helicases" without duplex unwinding
    Margaret E Fairman
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Science 304:730-4. 2004
    ..Therefore, the essential functions of DExH/D proteins are not confined to RNA duplexes but can be exerted on a wide range of ribonucleoprotein substrates...
  23. doi request reprint Duplex unwinding with DEAD-box proteins
    Eckhard Jankowsky
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA
    Methods Mol Biol 587:245-64. 2010
    ..In this chapter, we outline substrate design and unwinding protocols for DEAD-box proteins and focus on the quantitative evaluation of their unwinding activity...
  24. ncbi request reprint RNA helicases--one fold for many functions
    Eckhard Jankowsky
    Department of Biochemistry and Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Curr Opin Struct Biol 17:316-24. 2007
    ..Recent structural and functional studies have started to illuminate the mechanisms by which this remarkable diversity of functions can be conducted by the conserved helicase fold...
  25. pmc Angiogenin-Cleaved tRNA Halves Interact with Cytochrome c, Protecting Cells from Apoptosis during Osmotic Stress
    Mridusmita Saikia
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio, USA
    Mol Cell Biol 34:2450-63. 2014
    ..Our findings reveal a connection between angiogenin-generated tiRNAs and cell survival in response to hyperosmotic stress and suggest a novel cellular complex involving Cyt c and tiRNAs that inhibits apoptosome formation and activity. ..
  26. pmc Discovery of antivirulence agents against methicillin-resistant Staphylococcus aureus
    Varandt Khodaverdian
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    Antimicrob Agents Chemother 57:3645-52. 2013
    ..This represents a new use for an old drug. Antivirulence agents might be useful in prophylaxis and as adjuvants in antibiotic therapy for MRSA infections. ..