James W Jacobberger

Summary

Affiliation: Case Western Reserve University
Country: USA

Publications

  1. pmc Cell cycle-related cyclin b1 quantification
    Phyllis S Frisa
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, USA
    PLoS ONE 4:e7064. 2009
  2. pmc The kinetics of G2 and m transitions regulated by B cyclins
    Yehong Huang
    Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 8:e80861. 2013
  3. pmc Dynamic expression profiles from static cytometry data: component fitting and conversion to relative, "same scale" values
    Jayant Avva
    Department of Electrical Engineering and Computer Sciences, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 7:e38275. 2012
  4. pmc Dynamic epitope expression from static cytometry data: principles and reproducibility
    James W Jacobberger
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 7:e30870. 2012
  5. ncbi request reprint A new biomarker for mitotic cells
    James W Jacobberger
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Cytometry A 73:5-15. 2008
  6. ncbi request reprint Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activity
    James W Jacobberger
    Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106 4944, USA
    Cytometry A 54:75-88. 2003
  7. ncbi request reprint Cyclin B1 is rate limiting but not essential for mitotic entry and progression in mammalian somatic cells
    Deena V Soni
    Environmental Health Sciences, Case Western Reserve University, Cleveland, Ohio, USA
    Cell Cycle 7:1285-300. 2008
  8. pmc DNA synthesis from unbalanced nucleotide pools causes limited DNA damage that triggers ATR-CHK1-dependent p53 activation
    Kedar Hastak
    Department of Genetics and Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 105:6314-9. 2008
  9. pmc Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point
    Phyllis S Frisa
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
    BMC Cell Biol 11:26. 2010
  10. ncbi request reprint Inhibition of cdk1 by alsterpaullone and thioflavopiridol correlates with increased transit time from mid G2 through prophase
    Deena V Soni
    Environmental Health Sciences Department, Case Western Reserve University, Cleveland, Ohio, USA
    Cell Cycle 3:349-57. 2004

Collaborators

Detail Information

Publications30

  1. pmc Cell cycle-related cyclin b1 quantification
    Phyllis S Frisa
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, USA
    PLoS ONE 4:e7064. 2009
    ..Using a modified approach, we report quantification of endogenous cyclin B1 and generation of the cell cycle time related expression profile...
  2. pmc The kinetics of G2 and m transitions regulated by B cyclins
    Yehong Huang
    Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 8:e80861. 2013
    ..We discuss a simple model that would explain most data if cyclin B1 is necessary. ..
  3. pmc Dynamic expression profiles from static cytometry data: component fitting and conversion to relative, "same scale" values
    Jayant Avva
    Department of Electrical Engineering and Computer Sciences, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 7:e38275. 2012
    ..Here, we present a specific case of general methodology for calculating dynamic expression profiles of epitopes that oscillate during the cell cycle and conversion of these values to the same scale...
  4. pmc Dynamic epitope expression from static cytometry data: principles and reproducibility
    James W Jacobberger
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 7:e30870. 2012
    ..Here we present methodology that fulfills these features...
  5. ncbi request reprint A new biomarker for mitotic cells
    James W Jacobberger
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Cytometry A 73:5-15. 2008
    ..To facilitate use without confusion about specificity, we suggest the trivial name, pS780 for this mitotic epitope...
  6. ncbi request reprint Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activity
    James W Jacobberger
    Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106 4944, USA
    Cytometry A 54:75-88. 2003
    ..Here, we asked whether we could measure a phosphorylated epitope on Stat5 by cytometry, and whether that measurement would respond to Bcr/Abl inhibition...
  7. ncbi request reprint Cyclin B1 is rate limiting but not essential for mitotic entry and progression in mammalian somatic cells
    Deena V Soni
    Environmental Health Sciences, Case Western Reserve University, Cleveland, Ohio, USA
    Cell Cycle 7:1285-300. 2008
    ..Lack of uniformity in other phenotypes suggest that these are low penetrance effects that are exacerbated or compensated in some systems by other mechanisms...
  8. pmc DNA synthesis from unbalanced nucleotide pools causes limited DNA damage that triggers ATR-CHK1-dependent p53 activation
    Kedar Hastak
    Department of Genetics and Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 105:6314-9. 2008
    ....
  9. pmc Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction point
    Phyllis S Frisa
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
    BMC Cell Biol 11:26. 2010
    ..hTERT-BJ1, hTERT-RPE-1, and Molt4 cells were extracted with Triton X-100 followed by methanol fixation, stained with antibodies and DAPI, then measured by cytometry...
  10. ncbi request reprint Inhibition of cdk1 by alsterpaullone and thioflavopiridol correlates with increased transit time from mid G2 through prophase
    Deena V Soni
    Environmental Health Sciences Department, Case Western Reserve University, Cleveland, Ohio, USA
    Cell Cycle 3:349-57. 2004
    ..These results suggest that cdk1-cyclin activity is rate limiting for cell cycle progression during a period from mid G2 through prophase...
  11. ncbi request reprint CHK1 and CHK2 are differentially involved in mismatch repair-mediated 6-thioguanine-induced cell cycle checkpoint responses
    Tao Yan
    Department of Radiation Oncology, LTR 6068, University Hospitals of Cleveland Ireland Cancer Center, 11100 Euclid Avenue, Cleveland, OH 44106 6068, USA
    Mol Cancer Ther 3:1147-57. 2004
    ..The two signaling kinases seem to work cooperatively to ensure that 6-TG damaged cells arrest at these cell cycle checkpoints...
  12. pmc Major differences in the responses of primary human leukocyte subsets to IFN-beta
    Anette H H van Boxel-Dezaire
    Department of Molecular Genetics, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    J Immunol 185:5888-99. 2010
    ....
  13. pmc p53 negatively regulates intestinal immunity by delaying mucosal T cell cycling
    Andreas Sturm
    Division of Gastroenterology, Department of Medicine, University Hospitals of Cleveland, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4952, USA
    J Clin Invest 109:1481-92. 2002
    ..By slowing LPT cycling, p53 may act as a negative regulator of mucosal immunity, promoting immunological tolerance by preventing excessive T cell replication...
  14. doi request reprint CytoSys: a tool for extracting cell-cycle-related expression dynamics from static data
    Jayant Avva
    Case Western Reserve University, Cleveland, OH, USA
    Methods Mol Biol 717:171-93. 2011
    ....
  15. pmc High-resolution kinetics of cytokine signaling in human CD34/CD117-positive cells in unfractionated bone marrow
    Philip G Woost
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
    Blood 117:e131-41. 2011
    ..These data support the eventual use of this approach for disease diagnosis and monitoring...
  16. pmc Cell cycle arrest by kynurenine in lens epithelial cells
    Maneesh Mailankot
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio, USA
    Invest Ophthalmol Vis Sci 49:5466-75. 2008
    ..The molecular mechanisms by which KYNs cause cellular changes are poorly understood. The effects of KYNs on mouse lens epithelial cells by overexpression of human IDO were investigated...
  17. ncbi request reprint Phase II study of bryostatin 1 and vincristine for aggressive non-Hodgkin lymphoma relapsing after an autologous stem cell transplant
    Paul M Barr
    Department of Medicine, Case Comprehensive Cancer Center, University Hospitals Case Medical Center, Cleveland, Ohio 44106, USA
    Am J Hematol 84:484-7. 2009
    ..Future investigations of agents targeting the protein kinase C pathway may benefit from early response assessment using flow cytometry to evaluate T-cell apoptosis...
  18. doi request reprint Multiparameter cell cycle analysis
    James W Jacobberger
    Cytometry and Imaging Microscopy Core, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
    Methods Mol Biol 699:229-49. 2011
    ....
  19. doi request reprint Laser scanning cytometry of mitosis: state and stage analysis
    Tammy Stefan
    Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, USA
    Methods Cell Biol 102:341-72. 2011
    ..This platform allows both measurement of the cell states, capture of cell images within those states, and subsequent analysis of each image to classify by traditional mitotic stages based on nuclear morphology...
  20. ncbi request reprint Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood T lymphocytes
    Robin E Miller
    Department of Pediatrics, University Hospitals Ireland Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
    Exp Hematol 30:738-44. 2002
    ..CTLA-4 mediates negative T-cell signaling and may contribute to the development of allogeneic tolerance. In this study, we compared protein and mRNA expression of CTLA-4 in stimulated UCB and adult peripheral blood T cells...
  21. pmc A data-driven, mathematical model of mammalian cell cycle regulation
    Michael C Weis
    Department of Electrical Engineering and Computer Science, Case Western Reserve University, Cleveland, Ohio, United States of America
    PLoS ONE 9:e97130. 2014
    ..Since the method of data generation is extensible, this model can be continually modified based on new correlated, quantitative data. ..
  22. pmc Cell density related gene expression: SV40 large T antigen levels in immortalized astrocyte lines
    Phyllis S Frisa
    Comprehensive Cancer Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 4106 4944, USA
    BMC Cell Biol 3:10. 2002
    ..Here, we asked whether SV40 large T antigen (Tag) levels, driven by two different promoters, changed in a predictable and regular manner during exponential growth in clonal astrocyte cell lines, immortalized and dependent on Tag...
  23. pmc Monitoring Plasmodium falciparum growth and development by UV flow cytometry using an optimized Hoechst-thiazole orange staining strategy
    Brian T Grimberg
    Center for Global Health and Disease, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 7286, USA
    Cytometry A 73:546-54. 2008
    ..Investigation of the mechanisms by which anti-malarial drugs and antibodies act against different Pf lifecycle stages will be aided by this cytometric strategy...
  24. ncbi request reprint Phase I and correlative study of combination bryostatin 1 and vincristine in relapsed B-cell malignancies
    Afshin Dowlati
    Developmental Therapeutics Program and the Division of Hematology Oncology, Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
    Clin Cancer Res 9:5929-35. 2003
    ..Preclinical in vitro and in vivo studies demonstrate synergistic activity and increased tumor apoptosis in B-cell malignancies when a prolonged infusion of bryostatin 1 is followed by vincristine...
  25. ncbi request reprint Growth, immortalization, and differentiation potential of normal adult human proximal tubule cells
    David E Orosz
    Department of Physiology, Case Western Reserve University, Cleveland, Ohio 44106 4970, USA
    In Vitro Cell Dev Biol Anim 40:22-34. 2004
    ....
  26. ncbi request reprint The dietary isothiocyanate sulforaphane targets pathways of apoptosis, cell cycle arrest, and oxidative stress in human pancreatic cancer cells and inhibits tumor growth in severe combined immunodeficient mice
    Nhu An Pham
    Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Mol Cancer Ther 3:1239-48. 2004
    ..Our findings suggest that, in addition to the known effects on cancer prevention, sulforaphane may have activity in established pancreatic cancer...
  27. ncbi request reprint Dysregulated human myeloid nuclear differentiation antigen expression in myelodysplastic syndromes: evidence for a role in apoptosis
    Robert C Briggs
    Departments of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Cancer Res 66:4645-51. 2006
    ..We propose that MNDA has a role regulating programmed cell death in myeloid progenitor cells, and that its down-regulation in MDS is related to granulocyte-macrophage progenitor cell sensitivity to TRAIL-induced programmed cell death...
  28. ncbi request reprint Increasing the power of cytometry
    James W Jacobberger
    Nat Methods 3:343-4. 2006
  29. ncbi request reprint Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations
    Sue Chow
    Department of Pathology, Princess Margaret Hospital, Toronto, Ontario, Canada
    Cytometry A 67:4-17. 2005
    ..Detection of phospho-Thr 202/Tyr 204-p44/42 extracellular-regulated kinase (ERK) after phorbol ester acetate (PMA) stimulation was used as a model to measure phospho-epitopes in leukocyte populations in whole blood...
  30. ncbi request reprint Cytometry of the cell cycle: cycling through history
    Zbigniew Darzynkiewicz
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
    Cytometry A 58:21-32. 2004