Research Topics
Species | S L GersonSummaryAffiliation: Case Western Reserve University Country: USA Publications
| Collaborators
|
Detail Information
Publications
Leukocyte O6-alkylguanine-DNA alkyltransferase from human donors is uniformly sensitive to O6-benzylguanineS L Gerson
Division of Hematology Oncology and the Ireland Cancer Center at Case Western Reserve University and University Hospital of Cleveland, Ohio 44106, USA
Clin Cancer Res 5:521-4. 1999....- Clinical relevance of MGMT in the treatment of cancerStanton L Gerson
Division of Hematology Oncology and Comprehensive Cancer Center, University Hospitals of Cleveland, Case Western Reserve University School of Medicine, Cleveland, OH 44106 4937, USA
J Clin Oncol 20:2388-99. 2002..MGMT remains a paradigm for development of new agents that modulate known mechanisms of drug resistance in cancer cells and raise the spectra of combinatorial therapies that encompass known drug resistance mechanisms... - Transgenic expression of human MGMT blocks the hypersensitivity of PMS2-deficient mice to low dose MNU thymic lymphomagenesisX Qin
Division of Hematology Oncology and the Ireland Cancer Center, Case Western Reserve University, Cleveland, OH 44106 4937, USA
Carcinogenesis 20:1667-73. 1999.... - Sequential tumor biopsies in early phase clinical trials of anticancer agents for pharmacodynamic evaluationA Dowlati
Division of Hematology Oncology, Department of Medicine, Ireland Cancer Center at University Hospitals of Cleveland, 11100 Euclid Avenue, Cleveland, OH 44106, USA
Clin Cancer Res 7:2971-6. 2001..A major obstacle to target-based (often cytostatic) drug development has been obtaining relevant tumor tissue during clinical trials of these novel agents for laboratory analysis of the putative marker of drug effect... - Overexpression of human O6-alkylguanine DNA alkyltransferase (AGT) prevents MNU induced lymphomas in heterozygous p53 deficient miceJ S Reese
Division of Hematology Oncology, University Hospitals of Cleveland, Cleveland, Ohio, OH 44106, USA
Oncogene 20:5258-63. 2001..These data demonstrate that unrepaired O6mG lesions act cooperatively with the reduced p53 dose and lead to lymphomagenesis in p53+/- mice, but AGT overexpression and rapid removal of O6mG adducts is protective... - Randomized cross-over trial of progenitor-cell mobilization: high-dose cyclophosphamide plus granulocyte colony-stimulating factor (G-CSF) versus granulocyte-macrophage colony-stimulating factor plus G-CSFO N Koc
Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
J Clin Oncol 18:1824-30. 2000.... - Human long-term culture initiating cells are sensitive to benzylguanine and 1,3-bis(2-chloroethyl)-1-nitrosourea and protected after mutant (G156A) methylguanine methyltransferase gene transferO N Koc
Division of Hematology Oncology, Case Western Reserve University University Hospitals Ireland Cancer Center, Cleveland, Ohio 44106, USA
Cancer Gene Ther 6:340-8. 1999.... - MMTV promoter hypomethylation is linked to spontaneous and MNU associated c-neu expression and mammary carcinogenesis in MMTV c-neu transgenic miceH Zhou
Division of Hematology Oncology and Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106 4937, USA
Oncogene 20:6009-17. 2001..Expression of transgenic c-neu from the demethylated MMTV promoter appears to be an early event that allows outgrowth of mammary epithelium predisposed to malignant transformation... - Base excision repair as a therapeutic target in colon cancerLili Liu
Division of Hematology and Oncology, Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
Clin Cancer Res 8:2985-91. 2002..DNA BER may be a useful pharmacological target through which tumor cells can be sensitized to alkylating therapeutic agents... - Heterozygous DNA mismatch repair gene PMS2-knockout mice are susceptible to intestinal tumor induction with N-methyl-N-nitrosoureaX Qin
Division of Hematology Oncology and Ireland Cancer Center, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, OH 44106 4937, USA
Carcinogenesis 21:833-8. 2000..Organ-specific factors might influence MMR- mediated resistance to methylating agents. Heterozygous PMS2 knockout mice may be used as a promising animal model for intestinal tumorigenesis studies involving environmental carcinogens... - Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanineL Liu
Division of Hematology and Oncology, Department of Medicine, University Hospitals of Cleveland and Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-4937, USA
Clin Cancer Res 7:2318-24. 2001.... 
Removal of uracil by uracil DNA glycosylase limits pemetrexed cytotoxicity: overriding the limit with methoxyamine to inhibit base excision repairA D Bulgar
Department of Medicine, Division of Hematology Oncology, Case Western Reserve University, Cleveland, OH, USA
Cell Death Dis 3:e252. 2012..Thus, targeting BER-based therapy exhibits more selective cytotoxicity on cancer cells through a synthetic lethal mechanism...- Applied molecular evolution of O6-benzylguanine-resistant DNA alkyltransferases in human hematopoietic cellsB M Davis
Division of Hematology/Oncology, Molecular Virology Training Program, and the Comprehensive Cancer Center at Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106-4937, USA
Proc Natl Acad Sci U S A 98:4950-4. 2001..Of these, one was recovered significantly more frequently than the others. Upon further analysis, this mutant displayed a level of BG resistance in human hematopoietic cells greater than that of any previously reported mutant... - Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapyO N Koc
Departments of Medicine and Biology, Case Western Reserve University, Cleveland, OH 44106, USA
J Clin Oncol 18:307-16. 2000.... - Temozolomide: the effect of once- and twice-a-day dosing on tumor tissue levels of the DNA repair protein O(6)-alkylguanine-DNA-alkyltransferaseT P Spiro
Hematology Oncology Division, Department of Medicine, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106, USA
Clin Cancer Res 7:2309-17. 2001..Drug combinations or schedules designed to maximally deplete tumor AGT might improve TMZ efficacy... - MGMT expression in murine bone marrow is a major determinant of animal survival after alkylating agent exposureJ S Reese
Division of Hematology/Oncology, Case Western Reserve University and University Hospitals, Ireland Cancer Center, Cleveland, OH 44106, USA
J Hematother Stem Cell Res 10:115-23. 2001..Thus, DNA repair involving AGT in hematopoietic cells is required for normal host survival following exposure to methylating and chloroethylating agents... - Time-dependent effect of non-Hodgkin's lymphoma grade on disease-free survival of relapsed/refractory patients treated with high-dose chemotherapy plus autotransplantationP Fu
Department of Epidemiology and Biostatistics, Case Comprehensive Cancer Center, Cleveland, Ohio 44106, United States
Contemp Clin Trials 29:157-64. 2008..65). The time-dependent effect of lymphoma grade on disease-free survival suggests the need for early (within first year) incorporation of novel therapeutic approaches in management of patients with indolent NHL undergoing autotransplant... - High-dose carmustine, etoposide, and cisplatin for autologous stem cell transplantation with or without involved-field radiation for relapsed/refractory lymphoma: an effective regimen with low morbidity and mortalityPunit D Wadhwa
Department of Medicine, Comprehensive Cancer Center of the University Hospitals of Cleveland/Case Western Reserve University, Cleveland, Ohio 44106, USA
Biol Blood Marrow Transplant 11:13-22. 2005..BEP with or without IFR is a highly effective and well-tolerated regimen in the relapsed/refractory lymphoma setting. It has low morbidity and transplant-related mortality and a low incidence (3%) of posttransplantation malignancy... - Myeloablation is not required to select and maintain expression of the drug-resistance gene, mutant MGMT, in primary and secondary recipientsJanice E Bowman
Division of Hematology-Oncology and the Comprehensive Cancer Center, University Hospitals of Cleveland and Case Western Reserve University School of Medicine, 10900 Euclid Avenue, BRB 3-West, Cleveland, Ohio 44106-4937, USA
Mol Ther 8:42-50. 2003..Thus, MFG mutant MGMT vectors transduce repopulating hematopoietic stem cells that may be used both for chemotherapeutic drug resistance and to enrich for second therapeutic genes... - Systemic deficits in transporter for antigen presentation (TAP)-1 or proteasome subunit LMP2 have little or no effect on tumor incidenceA K Johnsen
Department of Pathology, Case Western Reserve University, Cleveland, OH, USA
Int J Cancer 91:366-72. 2001.... - Retroviral transduction and expression of the human alkyltransferase cDNA provides nitrosourea resistance to hematopoietic cellsJ A Allay
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4937, USA
Blood 85:3342-51. 1995..Overexpression of the alkyltransferase protein may protect hematopoietic progenitors from nitrosourea-induced myelosuppression... - Allogeneic mesenchymal stem cell infusion for treatment of metachromatic leukodystrophy (MLD) and Hurler syndrome (MPS-IH)O N Koc
Division of Hematology Oncology, Department of Medicine at Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106, USA
Bone Marrow Transplant 30:215-22. 2002..We conclude that donor allogeneic MSC infusion is safe and may be associated with reversal of disease pathophysiology in some tissues. The role of MSCs in the management of Hurler syndrome and MLD should be further evaluated... - Pharmacokinetics of O6-benzylguanine (NSC637037) and its metabolite, 8-oxo-O6-benzylguanineKou-Yi Tserng
Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Departments of Nutrition, Medicine, Mathematics, and Pharmacology, Case Western Reserve University, Ireland Cancer Center, University Hospitals of Cleveland, Cleveland, Ohio, USA
J Clin Pharmacol 43:881-93. 2003..This effect is not altered by coadministration of BCNU. The urinary excretion of drug and metabolites is minimal... - Bone marrow-derived mesenchymal stem cells remain host-derived despite successful hematopoietic engraftment after allogeneic transplantation in patients with lysosomal and peroxisomal storage diseasesO N Koc
Department of Medicine, University of Hospitals of Cleveland, OH, USA
Exp Hematol 27:1675-81. 1999..Whether culture expanded normal MSCs can be successfully transplanted into patients with storage diseases and provide therapeutic benefit needs to be determined... - DeltaMGMT-transduced bone marrow infusion increases tolerance to O6-benzylguanine and 1,3-bis(2-chloroethyl)-1-nitrosourea and allows intensive therapy of 1,3-bis(2-chloroethyl)-1-nitrosourea-resistant human colon cancer xenograftsO N Koc
Division of Hematology Oncology and the Ireland Cancer Center of University Hospitals of Cleveland and Case Western Reserve University, OH 44106, USA
Hum Gene Ther 10:1021-30. 1999.... - Hematopoietic engraftment and survival in adult recipients of umbilical-cord blood from unrelated donorsM J Laughlin
Department of Medicine, Ireland Cancer Center at University Hospitals of Cleveland and Case Western Reserve University, OH 44106 5065, USA
N Engl J Med 344:1815-22. 2001..We studied the use of transplantation of umbilical-cord blood to restore hematopoiesis in adults... - A phase I and pharmacodynamic study of fludarabine, carboplatin, and topotecan in patients with relapsed, refractory, or high-risk acute leukemiaBrenda W Cooper
Department of Medicine, University Hospitals of Cleveland, Ireland Cancer Center, Case Western Reserve University, Cleveland, Ohio 44109, USA
Clin Cancer Res 10:6830-9. 2004..A novel regimen designed to maximize antileukemia activity of carboplatin through inhibiting repair of platinum-DNA adducts was conducted in poor prognosis, acute leukemia patients... - Blockage of abasic site repair enhances antitumor efficacy of 1,3-bis-(2-chloroethyl)-1-nitrosourea in colon tumor xenograftsLili Liu
Division of Hematology and Oncology, Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Mol Cancer Ther 2:1061-6. 2003..Thus, targeting BER with MX is a promising strategy to improve the antitumor activity of BCNU and perhaps other DNA-damaging agents... - Combined treatment with temozolomide and methoxyamine: blocking apurininc/pyrimidinic site repair coupled with targeting topoisomerase IIalphaLing Yan
Department of Medicine, Division of Hematology Oncology, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
Clin Cancer Res 13:1532-9. 2007..When located within topoisomerase IIalpha (topo II) cleavage sites in DNA, MX-AP sites act as dual lethal targets, not only functionally disrupting the base excision repair (BER) pathway but also potentially poisoning topo II... - O6-benzylguanine and BCNU in multiple myeloma: a phase II trialEric D Batts
Developmental Therapeutics Program, CASE Comprehensive Cancer Center and the Division of Hematology Oncology, Case Western Reserve University, Cleveland, OH 44106, USA
Cancer Chemother Pharmacol 60:415-21. 2007..The objective of this study was to determine the safety and efficacy of depletion of MGMT activity in plasma cells using O6-benzylguanine (O6-BG) with BCNU in patients with multiple myeloma... - Phase I and correlative study of combination bryostatin 1 and vincristine in relapsed B-cell malignanciesAfshin Dowlati
Developmental Therapeutics Program and the Division of Hematology Oncology, Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
Clin Cancer Res 9:5929-35. 2003..Preclinical in vitro and in vivo studies demonstrate synergistic activity and increased tumor apoptosis in B-cell malignancies when a prolonged infusion of bryostatin 1 is followed by vincristine... - Therapeutic impact of methoxyamine: blocking repair of abasic sites in the base excision repair pathwayLili Liu
Division of Hematology and Oncology, Department of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Curr Opin Investig Drugs 5:623-7. 2004..This review will establish the premise that targeting abasic sites formed during BER will disrupt this pathway and enhance the therapeutic efficacy of alkylating agents... - Chemotherapy-induced O(6)-benzylguanine-resistant alkyltransferase mutations in mismatch-deficient colon cancerLili Liu
Division of Hematology and Oncology, Department of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4937, USA
Cancer Res 62:3070-6. 2002..These results suggest that K165 AGT mutants arising in MMR-deficient tumor cells after treatment with chemotherapeutic agents are both resistant to BG-inactivation and are active in the repair of alkylated DNA adducts... - A phase I and pharmacodynamic study of sequential topotecan and etoposide in patients with relapsed or refractory acute myelogenous and lymphoblastic leukemiaBrenda W Cooper
Department of Medicine, University Hospitals of Cleveland, Cleveland, OH 44106, USA
Leuk Res 27:35-44. 2003..The predicted up-regulation of topoisomerase II activity by topoisomerase I inhibition was not observed at this dose and schedule and may provide insight into the modest anti-leukemia activity of the regimen... - Hematologic effects of linezolid: summary of clinical experienceStanton L Gerson
Division of Hematology Oncology and Ireland Cancer Center, Case Western Reserve University, University Hospitals of Cleveland, 10900 Euclid Avenue, Cleveland, OH 44106 4937, USA
Antimicrob Agents Chemother 46:2723-6. 2002..Hematologic abnormalities were consistent with mild, reversible, duration-dependent myelosuppression. Appropriate monitoring is warranted with linezolid use... - Prospective study of one- vs two-unit umbilical cord blood transplantation following reduced intensity conditioning in adults with hematological malignanciesT L Kindwall-Keller
Department of Medicine, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA
Bone Marrow Transplant 47:924-33. 2012..Taken together, one-unit UCB transplantation with a threshold cell dose 2.5 × 10(7)/kg recipient weight after RIC is a viable option for adults, although infusion of two units confers a lower relapse incidence... - Hematopoietic stem cell gene therapy: progress toward therapeutic targetsJ L Vollweiler
Division of Hematology-Oncology, Comprehensive Cancer Center at University Hospitals of Cleveland, Case Western Reserve University School of Medicine, USA
Bone Marrow Transplant 32:1-7. 2003..The therapeutic potential of hematopoietic stem cell gene therapy, particularly given recent advances in stem cell plasticity, remains an exceptionally exciting area of clinical research... - Mice defective in the DNA mismatch gene PMS2 are hypersensitive to MNU induced thymic lymphoma and are partially protected by transgenic expression of human MGMTX Qin
Division of Hematology Oncology, Case Western Reserve University, Cleveland, Ohio, OH 44106 4937, USA
Oncogene 18:4394-400. 1999..These data suggest that PMS2 defective lymphomas may arise by the concerted action of environmental and perhaps endogenous methylation of DNA coupled to genomic instability... - Phase I trial of fludarabine, bortezomib and rituximab for relapsed and refractory indolent and mantle cell non-Hodgkin lymphomaPaul M Barr
Department of Medicine, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
Br J Haematol 147:89-96. 2009..Cumulative myelosuppression and neuropathy was observed. The combination of fludarabine, bortezomib, and rituximab appears to be an active regimen with manageable toxicity for relapsed NHL... - MGMT: its role in cancer aetiology and cancer therapeuticsStanton L Gerson
Case Comprehensive Cancer Center, University Hospitals of Cleveland and Case Western Reserve University, 10900 Euclid Ave, Cleveland, Ohio 44106, USA
Nat Rev Cancer 4:296-307. 2004 
Direct detection and quantification of abasic sites for in vivo studies of DNA damage and repairYanming Wang
Division of Radiopharmaceutical Science, Case Center for Imaging Research, Department of Radiology, Case Western Reserve University, Cleveland, OH 44122, USA
Nucl Med Biol 36:975-83. 2009....- Differential sensitivity of human and mouse alkyltransferase to O6-benzylguanine using a transgenic modelL Liu
Division of Hematology and Oncology, Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4937
Cancer Res 56:1880-5. 1996..This may influence the therapeutic index of O6-bG-BCNU combinations observed in human tumor xenograft-bearing mice... - Pre-mobilization therapy blood CD34+ cell count predicts the likelihood of successful hematopoietic stem cell mobilizationP Fu
Department of Epidemiology and Biostatistics, University Hospitals of Cleveland, Cleveland, OH 44106, USA
Bone Marrow Transplant 38:189-96. 2006..Prospectively identifying individuals at risk for mobilization failure would allow for improved treatment planning, resource utilization and time saving... - Repopulating defect of mismatch repair-deficient hematopoietic stem cellsJane S Reese
BRB 3, 10900 Euclid Ave, Cleveland, OH 44106, USA
Blood 102:1626-33. 2003..Thus, mismatch repair deficiency is associated with a hematopoietic repopulation defect and stem cell exhaustion because of accumulation of genomic instability... - Cancer gene therapy: scientific basisPunit D Wadhwa
Division of Hematology Oncology and Comprehensive Cancer Center, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio 44106 4937, USA
Annu Rev Med 53:437-52. 2002..Although no cures can consistently be expected from today's cancer gene therapy, the rapid progress may imply that such cures are a few short years away... - In vivo selection of MGMT(P140K) lentivirus-transduced human NOD/SCID repopulating cells without pretransplant irradiation conditioningSteven P Zielske
Molecular Virology Program, Case Western Reserve University, Cleveland, Ohio 44106, USA
J Clin Invest 112:1561-70. 2003..To our knowledge, these results provide the first evidence of potent in vivo selection of MGMT(P140K) lentivirus-transduced human SRCs following BG/BCNU treatment... - SarCNU mediates selection of P140K methylguanine-DNA-methyltransferase transduced human CD34(+) cells in vitroSteven P Zielske
Molecular Virology Program, Division of Hematology/Oncology and Comprehensive Cancer Center, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106, USA
Blood Cells Mol Dis 31:48-50. 2003..The IC(90) was increased sixfold by P140K MGMT transduction. These results suggest that SarCNU may be a suitable agent for in vivo selection strategies... - Drug selection of mutant methylguanine methyltransferase from different oncoretroviral backbones results in multilineage hematopoietic transgene expression in primary and secondary recipientsBrian M Davis
Division of Hematology/Oncology and the Comprehensive Cancer Center at Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106-4937, USA
J Hematother Stem Cell Res 12:375-87. 2003..Regardless of the vector backbone used, multiorgan expression was observed without evidence of gene silencing. These data help establish mutant, BG-resistant MGMT as a potent selection gene for stem cell selection in vivo... - Limited lentiviral transgene expression with increasing copy number in an MGMT selection model: lack of copy number selection by drug treatmentSteven P Zielske
Division of Hematology/Oncology, Case Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine, Case School of Medicine, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106, USA
Mol Ther 9:923-31. 2004..Thus drug selection would not be expected to increase the risk of oncogenesis due to exaggerated selection in favor of high-copy-number vector integration... - An unusual case of autoimmune hemolytic anemia with reticulocytopenia, erythroid dysplasia, and an IgG2 autoanti-UG R Roush
Department of Pathology, Case Western Reserve University, Cleveland, Ohio, USA
Transfusion 36:575-80. 1996..Early treatment with U- blood, in addition to steroids, may be beneficial... - Measurement of anti-cancer agent methoxyamine in plasma by tandem mass spectrometry with on-line sample extractionShuming Yang
Department of Chemistry, Cleveland State University, Cleveland, OH 44115-2406, USA
J Chromatogr B Analyt Technol Biomed Life Sci 795:295-307. 2003..This method has been used to analyze the plasma levels of methoxyamine in samples obtained from male CD1 mice after bolus intraperitoneal injection of 2, 5 and 20mg methoxyamine hydrochloride (CH3ONH2.HCl) per kilogram mouse... - Targeted modulation of MGMT: clinical implicationsLili Liu
Department of Medicine, Division of Hematology/Oncology, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, USA
Clin Cancer Res 12:328-31. 2006..Because of its role in cancer etiology and chemotherapy resistance, MGMT is of particular interest. In this article, the clinical effect of MGMT expression and targeted modulation of MGMT will be summarized... - DNA repair defects in stem cell function and agingYoungji Park
Division of Hematology and Oncology, University Hospitals of Cleveland, Case Western Reserve University, Cleveland, Ohio 44106 4937, USA
Annu Rev Med 56:495-508. 2005..This discovery raises the possibility of a link between aging and stem cell dysfunction. In this review, we provide evidence for a link between DNA repair systems and the maintenance and longevity of stem cells... - Lentiviral transduction of P140K MGMT into human CD34(+) hematopoietic progenitors at low multiplicity of infection confers significant resistance to BG/BCNU and allows selection in vitroSteven P Zielske
Molecular Virology Program, Division of Hematology/Oncology and Comprehensive Cancer Center, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
Mol Ther 5:381-7. 2002..Because drug selection will enrich for transduced progenitors, high MOI can be avoided, improving the safety profile of lentiviral gene transfer... - Cytokines, including stem cell factor alone, enhance lentiviral transduction in nondividing human LTCIC and NOD/SCID repopulating cellsSteven P Zielske
Molecular Virology Program, Division of Hematology/Oncology, and Comprehensive Cancer Center, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
Mol Ther 7:325-33. 2003..These data suggest that cytokines enhance lentiviral transduction of HSC, without requiring cell division, and maintain the stem cell phenotype. SCF stimulation alone was sufficient for high level transduction... - The role of DNA damage repair in aging of adult stem cellsJonathan Kenyon
Case Western Reserve University and the Ireland Cancer Center, University Hospitals Case Medical Center, Cleveland, OH 44106, USA
Nucleic Acids Res 35:7557-65. 2007..Human diseases and animal models of loss of DNA repair provide longitudinal analysis of DNA repair processes in stem cell populations and may provide links to the physiology of aging... - Bone marrow-derived cells exhibiting lung epithelial cell characteristics are enriched in vivo using methylguanine DNA methyltransferase-mediated drug resistanceJane S Reese
Case Comprehensive Cancer Center, Case Western Reserve University, 11100 Euclid Avenue, Cleveland, Ohio 44106, USA
Stem Cells 26:675-81. 2008..These results suggest that expression of MGMT could enhance the capacity of bone marrow-derived cells to repopulate lung epithelium, and when used in combination with a gene of interest, MGMT could have therapeutic applications... - Telomerase inhibition and telomere erosion: a two-pronged strategy in cancer therapyFred O Odago
Division of Hematology/Oncology, Comprehensive Cancer Center, Case Western Reserve University and University Hospitals of Cleveland, 10900 Euclid Avenue, BRB 3-West, Cleveland, OH 44106-4937, USA
Trends Pharmacol Sci 24:328-31. 2003 - Identification of 6-benzylthioinosine as a myeloid leukemia differentiation-inducing compoundDavid N Wald
Department of Pathology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106, USA
Cancer Res 68:4369-76. 2008..Besides in vitro activity, 6BT also shows the ability to impair HL-60 and MV4-11 tumor growth in nude mice. 6BT is a promising new monocytic differentiation agent with apparent leukemic cell-specific activity... - Phase I trial of VNP40101M (Cloretazine) in children with recurrent brain tumors: a pediatric brain tumor consortium studySridharan Gururangan
The Preston Robert Tisch Brain Tumor Center, Duke University Medical Center, Durham, North Carolina 27710, USA
Clin Cancer Res 14:1124-30. 2008..VNP40101M (Cloretazine), a novel DNA alkylating agent, was evaluated in a phase I study in children with recurrent brain tumors... - Phase II trial of the O6-alkylguanine DNA alkyltransferase inhibitor O6-benzylguanine and 1,3-bis(2-chloroethyl)-1-nitrosourea in advanced melanomaThomas F Gajewski
Department of Medicine, University of Chicago, IL 60637, USA
Clin Cancer Res 11:7861-5. 2005..We postulated that the addition of O(6) benzylguanine (O(6)BG), a potent inactivator of AGT, would improve the clinical response to BCNU in melanoma... - N-benzoylstaurosporine (PKC412) inhibits Akt kinase inducing apoptosis in multiple myeloma cellsNizar J Bahlis
University of Calgary, Department of Medicine, Division of Hematology and Hematological Malignancies, Calgary, Alberta, Canada
Leuk Lymphoma 46:899-908. 2005..PKC412 is an appropriate candidate for novel treatment protocols for multiple myeloma... - A randomized phase I and pharmacological trial of sequences of 1,3-bis(2-chloroethyl)-1-nitrosourea and temozolomide in patients with advanced solid neoplasmsLisa A Hammond
Cancer Therapy and Research Center, University of San Antonio Health Science Center at San Antonio, San Antonio, Texas, USA
Clin Cancer Res 10:1645-56. 2004..The characteristics of the myelosuppressive effects of BCNU/TEM, the paucity of severe nonhematological toxicities, and antitumor activity at tolerable doses warrant disease-directed evaluations on this schedule... - A bicistronic SIN-lentiviral vector containing G156A MGMT allows selection and metabolic correction of hematopoietic protoporphyric cell linesEmmanuel Richard
INSERM E0217, , France
J Gene Med 5:737-47. 2003..This vector might form the basis of new EPP mouse gene therapy protocols without a preconditioning regimen followed by in vivo selection of corrected hematopoietic stem cells... - Long-term-infected telomerase-immortalized endothelial cells: a model for Kaposi's sarcoma-associated herpesvirus latency in vitro and in vivoFeng-Qi An
Department of Molecular Genetics and Microbiology, University of Florida, Shands Cancer Center, Gainesville, FL 32610-0232, USA
J Virol 80:4833-46. 2006..KSHV-infected LTCs represent the first xenograft model for KS and should be of use to study KS pathogenesis and for the validation of anti-KS drug candidates... - Differential competitive resistance to methylating versus chloroethylating agents among five O6-alkylguanine DNA alkyltransferases in human hematopoietic cellsAparecida Maria Fontes
Division of Hematology/Oncology and Comprehensive Cancer Center, Case Western Reserve University and University Hospitals of Cleveland, OH 44106-4955, USA
Mol Cancer Ther 5:121-8. 2006..The optimal mutant MGMT useful as a drug resistance gene may depend on whether a methylating or chloroethylating agent is used for drug selection... - Bioluminescence imaging of hematopoietic stem cell repopulation in murine modelsYuan Lin
Department of Medicine, Division of Hematology Oncology, Case Western Reserve University, Cleveland, OH
Methods Mol Biol 430:295-306. 2008..Xenogen IVIS200 imaging system is used to record the process, and other similar imaging systems can also be used for this process... - Adult bone marrow stem cells for cell and gene therapies: implications for greater useChristopher B Ballas
Division of Hematology/Oncology, Comprehensive Cancer Center at University Hospitals of Cleveland and Case Western Reserve University, Ohio, USA
J Cell Biochem Suppl 38:20-8. 2002..Recent information indicates HSCs, MSCs, and other cells in general may have more universal differentiation abilities than previously thought... - Randomised comparison of two B-cell purging protocols for patients with B-cell non-Hodgkin lymphoma: in vivo purging with rituximab versus ex vivo purging with CliniMACS CD34 cell enrichment deviceWillem J van Heeckeren
Department of Medicine, Case Western Reserve University and University Hospitals of Cleveland, OH, USA
Br J Haematol 132:42-55. 2006..Further studies are needed to determine whether rituximab purging is more effective than the use of unpurged autografts...