Phyllis S Frisa
Affiliation: Case Western Reserve University
- Estimation of kinetic cell-cycle-related gene expression in G1 and G2 phases from immunofluorescence flow cytometry dataJ W Jacobberger
Cancer Research Center, Case Western Reserve University, Cleveland, Ohio 44106 4944, USA
Cytometry 35:284-9. 1999..We present an objective method to model G1 and G2 kinetic expression from an estimate of a minimum biological unit of positive immunofluorescence derived from the distribution of specific immunofluorescence of mitotic cells...
- Cell density related gene expression: SV40 large T antigen levels in immortalized astrocyte linesPhyllis S Frisa
Comprehensive Cancer Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 4106 4944, USA
BMC Cell Biol 3:10. 2002..Here, we asked whether SV40 large T antigen (Tag) levels, driven by two different promoters, changed in a predictable and regular manner during exponential growth in clonal astrocyte cell lines, immortalized and dependent on Tag...
- Cell cycle-related cyclin b1 quantificationPhyllis S Frisa
Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, USA
PLoS ONE 4:e7064. 2009..Using a modified approach, we report quantification of endogenous cyclin B1 and generation of the cell cycle time related expression profile...
- A new biomarker for mitotic cellsJames W Jacobberger
Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
Cytometry A 73:5-15. 2008..To facilitate use without confusion about specificity, we suggest the trivial name, pS780 for this mitotic epitope...
- Cytometry of chromatin bound Mcm6 and PCNA identifies two states in G1 that are separated functionally by the G1 restriction pointPhyllis S Frisa
Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
BMC Cell Biol 11:26. 2010..hTERT-BJ1, hTERT-RPE-1, and Molt4 cells were extracted with Triton X-100 followed by methanol fixation, stained with antibodies and DAPI, then measured by cytometry...
- DNA synthesis from unbalanced nucleotide pools causes limited DNA damage that triggers ATR-CHK1-dependent p53 activationKedar Hastak
Department of Genetics and Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
Proc Natl Acad Sci U S A 105:6314-9. 2008....
- Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activityJames W Jacobberger
Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio 44106 4944, USA
Cytometry A 54:75-88. 2003..Here, we asked whether we could measure a phosphorylated epitope on Stat5 by cytometry, and whether that measurement would respond to Bcr/Abl inhibition...
- CHK1 and CHK2 are differentially involved in mismatch repair-mediated 6-thioguanine-induced cell cycle checkpoint responsesTao Yan
Department of Radiation Oncology, LTR 6068, University Hospitals of Cleveland Ireland Cancer Center, 11100 Euclid Avenue, Cleveland, OH 44106 6068, USA
Mol Cancer Ther 3:1147-57. 2004..The two signaling kinases seem to work cooperatively to ensure that 6-TG damaged cells arrest at these cell cycle checkpoints...
- Phase I and correlative study of combination bryostatin 1 and vincristine in relapsed B-cell malignanciesAfshin Dowlati
Developmental Therapeutics Program and the Division of Hematology Oncology, Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
Clin Cancer Res 9:5929-35. 2003..Preclinical in vitro and in vivo studies demonstrate synergistic activity and increased tumor apoptosis in B-cell malignancies when a prolonged infusion of bryostatin 1 is followed by vincristine...
- Major differences in the responses of primary human leukocyte subsets to IFN-betaAnette H H van Boxel-Dezaire
Department of Molecular Genetics, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
J Immunol 185:5888-99. 2010....
- p53 negatively regulates intestinal immunity by delaying mucosal T cell cyclingAndreas Sturm
Division of Gastroenterology, Department of Medicine, University Hospitals of Cleveland, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4952, USA
J Clin Invest 109:1481-92. 2002..By slowing LPT cycling, p53 may act as a negative regulator of mucosal immunity, promoting immunological tolerance by preventing excessive T cell replication...
- Inhibition of cdk1 by alsterpaullone and thioflavopiridol correlates with increased transit time from mid G2 through prophaseDeena V Soni
Environmental Health Sciences Department, Case Western Reserve University, Cleveland, Ohio, USA
Cell Cycle 3:349-57. 2004..These results suggest that cdk1-cyclin activity is rate limiting for cell cycle progression during a period from mid G2 through prophase...
- Growth, immortalization, and differentiation potential of normal adult human proximal tubule cellsDavid E Orosz
Department of Physiology, Case Western Reserve University, Cleveland, Ohio 44106 4970, USA
In Vitro Cell Dev Biol Anim 40:22-34. 2004....
- Divergent cell cycle kinetics underlie the distinct functional capacity of mucosal T cells in Crohn's disease and ulcerative colitisA Sturm
Department of Medicine, University Hospitals of Cleveland, Case Western Reserve University School of Medicine BRB 425, 10900 Euclid Ave, Cleveland, Ohio, 44106 4952, USA
Gut 53:1624-31. 2004..Because cell cycling determines effector function, pathogenic events in CD and UC may depend on cell cycle changes unique to each condition...
- Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood T lymphocytesRobin E Miller
Department of Pediatrics, University Hospitals Ireland Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH 44106, USA
Exp Hematol 30:738-44. 2002..CTLA-4 mediates negative T-cell signaling and may contribute to the development of allogeneic tolerance. In this study, we compared protein and mRNA expression of CTLA-4 in stimulated UCB and adult peripheral blood T cells...
- Phase II study of bryostatin 1 and vincristine for aggressive non-Hodgkin lymphoma relapsing after an autologous stem cell transplantPaul M Barr
Department of Medicine, Case Comprehensive Cancer Center, University Hospitals Case Medical Center, Cleveland, Ohio 44106, USA
Am J Hematol 84:484-7. 2009..Future investigations of agents targeting the protein kinase C pathway may benefit from early response assessment using flow cytometry to evaluate T-cell apoptosis...
- Cell cycle arrest by kynurenine in lens epithelial cellsManeesh Mailankot
Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio, USA
Invest Ophthalmol Vis Sci 49:5466-75. 2008..The molecular mechanisms by which KYNs cause cellular changes are poorly understood. The effects of KYNs on mouse lens epithelial cells by overexpression of human IDO were investigated...
- Cyclin B1 is rate limiting but not essential for mitotic entry and progression in mammalian somatic cellsDeena V Soni
Environmental Health Sciences, Case Western Reserve University, Cleveland, Ohio, USA
Cell Cycle 7:1285-300. 2008..Lack of uniformity in other phenotypes suggest that these are low penetrance effects that are exacerbated or compensated in some systems by other mechanisms...
- Monitoring Plasmodium falciparum growth and development by UV flow cytometry using an optimized Hoechst-thiazole orange staining strategyBrian T Grimberg
Center for Global Health and Disease, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 7286, USA
Cytometry A 73:546-54. 2008..Investigation of the mechanisms by which anti-malarial drugs and antibodies act against different Pf lifecycle stages will be aided by this cytometric strategy...
- The dietary isothiocyanate sulforaphane targets pathways of apoptosis, cell cycle arrest, and oxidative stress in human pancreatic cancer cells and inhibits tumor growth in severe combined immunodeficient miceNhu An Pham
Department of Medical Biophysics, University of Toronto, Ontario, Canada
Mol Cancer Ther 3:1239-48. 2004..Our findings suggest that, in addition to the known effects on cancer prevention, sulforaphane may have activity in established pancreatic cancer...
- Dysregulated human myeloid nuclear differentiation antigen expression in myelodysplastic syndromes: evidence for a role in apoptosisRobert C Briggs
Departments of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
Cancer Res 66:4645-51. 2006..We propose that MNDA has a role regulating programmed cell death in myeloid progenitor cells, and that its down-regulation in MDS is related to granulocyte-macrophage progenitor cell sensitivity to TRAIL-induced programmed cell death...
- Increasing the power of cytometryJames W Jacobberger
Nat Methods 3:343-4. 2006
- Cytometry of the cell cycle: cycling through historyZbigniew Darzynkiewicz
Brander Cancer Research Institute, New York Medical College, Valhalla, New York 10532, USA
Cytometry A 58:21-32. 2004
- Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulationsSue Chow
Department of Pathology, Princess Margaret Hospital, Toronto, Ontario, Canada
Cytometry A 67:4-17. 2005..Detection of phospho-Thr 202/Tyr 204-p44/42 extracellular-regulated kinase (ERK) after phorbol ester acetate (PMA) stimulation was used as a model to measure phospho-epitopes in leukocyte populations in whole blood...