Research Topics
| Pieter L deHasethSummaryAffiliation: Case Western Reserve University Country: USA Publications
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Detail Information
Publications
Conformational flexibility of sigma(70) in anti-terminator loadingPieter L deHaseth
RNA Center, Case Western Reserve University, Cleveland, OH 44106 4973, USA
Mol Microbiol 75:543-6. 2010..This attests to the great flexibility of the 3.2 linker. The particularly stable complex with the 2 bp separation may lend itself to structural studies of an early elongation complex containing sigma(70)...
Strand opening-deficient Escherichia coli RNA polymerase facilitates investigation of closed complexes with promoter DNA: effects of DNA sequence and temperatureVictoria M Cook
Center for RNA Molecular Biology, Case Western Reserve University, Clevland, Ohio 44106, USA
J Biol Chem 282:21319-26. 2007..This suggests that low temperature complexes of wild type RNA polymerase and promoter DNA may adequately model the closed complex...
Substitution of a highly conserved histidine in the Escherichia coli heat shock transcription factor, sigma32, affects promoter utilization in vitro and leads to overexpression of the biofilm-associated flu protein in vivoOlga V Kourennaia
Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland OH 44106 4973, USA
J Bacteriol 189:8430-6. 2007....- The -11A of promoter DNA and two conserved amino acids in the melting region of sigma70 both directly affect the rate limiting step in formation of the stable RNA polymerase-promoter complex, but they do not necessarily interactLisa A Schroeder
The Center for RNA Molecular Biology and The Department of Biochemistry, Case Western Reserve University, Cleveland, OH, USA
Nucleic Acids Res 35:4141-53. 2007..The effects of the substitutions at -11A of the promoter and Y430 and W433 of sigma70 are cumulative... - Mutational analysis of Escherichia coli heat shock transcription factor sigma 32 reveals similarities with sigma 70 in recognition of the -35 promoter element and differences in promoter DNA melting and -10 recognitionOlga V Kourennaia
Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4973, USA
J Bacteriol 187:6762-9. 2005..4 on the activities of the two sigma factors are consistent with the pronounced differences between both the amino acid sequences in this region and the recognized promoter DNA sequences... - Probing the role of region 2 of Escherichia coli sigma 70 in nucleation and maintenance of the single-stranded DNA bubble in RNA polymerase-promoter open complexesPieter L deHaseth
Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106-4935, USA
Methods Enzymol 370:553-67. 2003 - Threonine 429 of Escherichia coli sigma 70 is a key participant in promoter DNA melting by RNA polymeraseLisa A Schroeder
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106, USA
J Mol Biol 376:153-65. 2008..This model suggests an active role for RNA polymerase in disrupting the -11 base pair, rather than just capturing the -11A subsequent to spontaneous unpairing... - Evidence for a tyrosine-adenine stacking interaction and for a short-lived open intermediate subsequent to initial binding of Escherichia coli RNA polymerase to promoter DNALisa A Schroeder
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106 4973, USA
J Mol Biol 385:339-49. 2009..These data refine the structural model for open complex formation and reveal a novel interaction involved in DNA melting by RNA polymerase... - Molecular biology. When a part is as good as the wholePieter L deHaseth
RNA Center and Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA
Science 303:1307-8. 2004 - Mechanistic differences in promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerasesLisa A Schroeder
Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4973, USA
J Biol Chem 280:17422-9. 2005..A model is presented for how this mechanistic difference between the two RNA polymerase could explain our observations... - RNA polymerase alters the mobility of an A-residue crucial to polymerase-induced melting of promoter DNALaura Tsujikawa
Department of Biochemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA
Biochemistry 41:15334-41. 2002..These results are consistent with the residue at -11 being tightly bound in a hydrophobic pocket of the enzyme... - Sigma 32-dependent promoter activity in vivo: sequence determinants of the groE promoterYang Wang
Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4935, USA
J Bacteriol 185:5800-6. 2003..At each of the specified base pairs, substitutions were found which reduced promoter activity by greater than 75%. Activity was also dependent upon the number of base pairs separating the two regions... - Interaction of RNA polymerase with forked DNA: evidence for two kinetically significant intermediates on the pathway to the final complexLaura Tsujikawa
Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106-4935, USA
Proc Natl Acad Sci U S A 99:3493-8. 2002....