Pieter L deHaseth

Summary

Affiliation: Case Western Reserve University
Country: USA

Publications

  1. doi request reprint Conformational flexibility of sigma(70) in anti-terminator loading
    Pieter L deHaseth
    RNA Center, Case Western Reserve University, Cleveland, OH 44106 4973, USA
    Mol Microbiol 75:543-6. 2010
  2. ncbi request reprint Strand opening-deficient Escherichia coli RNA polymerase facilitates investigation of closed complexes with promoter DNA: effects of DNA sequence and temperature
    Victoria M Cook
    Center for RNA Molecular Biology, Case Western Reserve University, Clevland, Ohio 44106, USA
    J Biol Chem 282:21319-26. 2007
  3. pmc Substitution of a highly conserved histidine in the Escherichia coli heat shock transcription factor, sigma32, affects promoter utilization in vitro and leads to overexpression of the biofilm-associated flu protein in vivo
    Olga V Kourennaia
    Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland OH 44106 4973, USA
    J Bacteriol 189:8430-6. 2007
  4. pmc The -11A of promoter DNA and two conserved amino acids in the melting region of sigma70 both directly affect the rate limiting step in formation of the stable RNA polymerase-promoter complex, but they do not necessarily interact
    Lisa A Schroeder
    The Center for RNA Molecular Biology and The Department of Biochemistry, Case Western Reserve University, Cleveland, OH, USA
    Nucleic Acids Res 35:4141-53. 2007
  5. pmc Mutational analysis of Escherichia coli heat shock transcription factor sigma 32 reveals similarities with sigma 70 in recognition of the -35 promoter element and differences in promoter DNA melting and -10 recognition
    Olga V Kourennaia
    Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4973, USA
    J Bacteriol 187:6762-9. 2005
  6. ncbi request reprint Probing the role of region 2 of Escherichia coli sigma 70 in nucleation and maintenance of the single-stranded DNA bubble in RNA polymerase-promoter open complexes
    Pieter L deHaseth
    Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4935, USA
    Methods Enzymol 370:553-67. 2003
  7. pmc Threonine 429 of Escherichia coli sigma 70 is a key participant in promoter DNA melting by RNA polymerase
    Lisa A Schroeder
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106, USA
    J Mol Biol 376:153-65. 2008
  8. pmc Evidence for a tyrosine-adenine stacking interaction and for a short-lived open intermediate subsequent to initial binding of Escherichia coli RNA polymerase to promoter DNA
    Lisa A Schroeder
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106 4973, USA
    J Mol Biol 385:339-49. 2009
  9. ncbi request reprint Molecular biology. When a part is as good as the whole
    Pieter L deHaseth
    RNA Center and Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA
    Science 303:1307-8. 2004
  10. ncbi request reprint Mechanistic differences in promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerases
    Lisa A Schroeder
    Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4973, USA
    J Biol Chem 280:17422-9. 2005

Collaborators

Detail Information

Publications13

  1. doi request reprint Conformational flexibility of sigma(70) in anti-terminator loading
    Pieter L deHaseth
    RNA Center, Case Western Reserve University, Cleveland, OH 44106 4973, USA
    Mol Microbiol 75:543-6. 2010
    ..This attests to the great flexibility of the 3.2 linker. The particularly stable complex with the 2 bp separation may lend itself to structural studies of an early elongation complex containing sigma(70)...
  2. ncbi request reprint Strand opening-deficient Escherichia coli RNA polymerase facilitates investigation of closed complexes with promoter DNA: effects of DNA sequence and temperature
    Victoria M Cook
    Center for RNA Molecular Biology, Case Western Reserve University, Clevland, Ohio 44106, USA
    J Biol Chem 282:21319-26. 2007
    ..This suggests that low temperature complexes of wild type RNA polymerase and promoter DNA may adequately model the closed complex...
  3. pmc Substitution of a highly conserved histidine in the Escherichia coli heat shock transcription factor, sigma32, affects promoter utilization in vitro and leads to overexpression of the biofilm-associated flu protein in vivo
    Olga V Kourennaia
    Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland OH 44106 4973, USA
    J Bacteriol 189:8430-6. 2007
    ....
  4. pmc The -11A of promoter DNA and two conserved amino acids in the melting region of sigma70 both directly affect the rate limiting step in formation of the stable RNA polymerase-promoter complex, but they do not necessarily interact
    Lisa A Schroeder
    The Center for RNA Molecular Biology and The Department of Biochemistry, Case Western Reserve University, Cleveland, OH, USA
    Nucleic Acids Res 35:4141-53. 2007
    ..The effects of the substitutions at -11A of the promoter and Y430 and W433 of sigma70 are cumulative...
  5. pmc Mutational analysis of Escherichia coli heat shock transcription factor sigma 32 reveals similarities with sigma 70 in recognition of the -35 promoter element and differences in promoter DNA melting and -10 recognition
    Olga V Kourennaia
    Center for RNA Molecular Biology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106 4973, USA
    J Bacteriol 187:6762-9. 2005
    ..4 on the activities of the two sigma factors are consistent with the pronounced differences between both the amino acid sequences in this region and the recognized promoter DNA sequences...
  6. ncbi request reprint Probing the role of region 2 of Escherichia coli sigma 70 in nucleation and maintenance of the single-stranded DNA bubble in RNA polymerase-promoter open complexes
    Pieter L deHaseth
    Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4935, USA
    Methods Enzymol 370:553-67. 2003
  7. pmc Threonine 429 of Escherichia coli sigma 70 is a key participant in promoter DNA melting by RNA polymerase
    Lisa A Schroeder
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106, USA
    J Mol Biol 376:153-65. 2008
    ..This model suggests an active role for RNA polymerase in disrupting the -11 base pair, rather than just capturing the -11A subsequent to spontaneous unpairing...
  8. pmc Evidence for a tyrosine-adenine stacking interaction and for a short-lived open intermediate subsequent to initial binding of Escherichia coli RNA polymerase to promoter DNA
    Lisa A Schroeder
    Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH 44106 4973, USA
    J Mol Biol 385:339-49. 2009
    ..These data refine the structural model for open complex formation and reveal a novel interaction involved in DNA melting by RNA polymerase...
  9. ncbi request reprint Molecular biology. When a part is as good as the whole
    Pieter L deHaseth
    RNA Center and Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA
    Science 303:1307-8. 2004
  10. ncbi request reprint Mechanistic differences in promoter DNA melting by Thermus aquaticus and Escherichia coli RNA polymerases
    Lisa A Schroeder
    Center for RNA Molecular Biology and Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4973, USA
    J Biol Chem 280:17422-9. 2005
    ..A model is presented for how this mechanistic difference between the two RNA polymerase could explain our observations...
  11. ncbi request reprint RNA polymerase alters the mobility of an A-residue crucial to polymerase-induced melting of promoter DNA
    Laura Tsujikawa
    Department of Biochemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA
    Biochemistry 41:15334-41. 2002
    ..These results are consistent with the residue at -11 being tightly bound in a hydrophobic pocket of the enzyme...
  12. pmc Sigma 32-dependent promoter activity in vivo: sequence determinants of the groE promoter
    Yang Wang
    Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4935, USA
    J Bacteriol 185:5800-6. 2003
    ..At each of the specified base pairs, substitutions were found which reduced promoter activity by greater than 75%. Activity was also dependent upon the number of base pairs separating the two regions...
  13. pmc Interaction of RNA polymerase with forked DNA: evidence for two kinetically significant intermediates on the pathway to the final complex
    Laura Tsujikawa
    Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106 4935, USA
    Proc Natl Acad Sci U S A 99:3493-8. 2002
    ....