Allan Spradling

Summary

Affiliation: Carnegie Institution
Country: USA

Publications

  1. ncbi request reprint Developmental biology. The mother of all stem cells?
    Allan C Spradling
    Department of Embryology and Howard Hughes Medical Institute, Carnegie Institution, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Science 315:469-70. 2007
  2. pmc Ovulation in Drosophila is controlled by secretory cells of the female reproductive tract
    Jianjun Sun
    Department of Embryology, Carnegie Institution for Science, Baltimore, United States Howard Hughes Medical Institute, Baltimore, United States
    elife 2:e00415. 2013
  3. pmc Steroid signaling within Drosophila ovarian epithelial cells sex-specifically modulates early germ cell development and meiotic entry
    Lucy X Morris
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution, Baltimore, Maryland, United States of America
    PLoS ONE 7:e46109. 2012
  4. pmc Drosophila P elements preferentially transpose to replication origins
    Allan C Spradling
    Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 108:15948-53. 2011
  5. doi request reprint The living-tissue microscope: the importance of studying stem cells in their natural, undisturbed microenvironment
    Allan C Spradling
    Department of Embryology HHMI, Carnegie Institution, Carnegie Institution, Baltimore, MD 21218, USA
    J Pathol 225:161-2. 2011
  6. pmc Learning the common language of genetics
    Allan C Spradling
    Department of Embryology, Carnegie Institution of Washington HHMI, Baltimore, MD 21218, USA
    Genetics 174:1-3. 2006
  7. pmc New roles for model genetic organisms in understanding and treating human disease: report from the 2006 Genetics Society of America meeting
    Allan Spradling
    Department of Embryology HHMI, Carnegie Institution of Washington, Baltimore, MD 21218, USA
    Genetics 172:2025-32. 2006
  8. pmc Regulation of epithelial stem cell replacement and follicle formation in the Drosophila ovary
    Todd Nystul
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genetics 184:503-15. 2010
  9. ncbi request reprint Multipotent Drosophila intestinal stem cells specify daughter cell fates by differential notch signaling
    Benjamin Ohlstein
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Science 315:988-92. 2007
  10. ncbi request reprint Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries
    Toshie Kai
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, 115 W University Parkway, Baltimore, Maryland 21210, USA
    Nature 428:564-9. 2004

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Developmental biology. The mother of all stem cells?
    Allan C Spradling
    Department of Embryology and Howard Hughes Medical Institute, Carnegie Institution, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Science 315:469-70. 2007
  2. pmc Ovulation in Drosophila is controlled by secretory cells of the female reproductive tract
    Jianjun Sun
    Department of Embryology, Carnegie Institution for Science, Baltimore, United States Howard Hughes Medical Institute, Baltimore, United States
    elife 2:e00415. 2013
    ..Our results suggest that the reproductive tract signals to the ovary using glandular secretions, and that this pathway has been conserved during evolution. DOI:http://dx.doi.org/10.7554/eLife.00415.001...
  3. pmc Steroid signaling within Drosophila ovarian epithelial cells sex-specifically modulates early germ cell development and meiotic entry
    Lucy X Morris
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution, Baltimore, Maryland, United States of America
    PLoS ONE 7:e46109. 2012
    ..Our results suggest that steroid signaling plays an important sex-specific role in early germ cell development in Drosophila, a strategy that may be conserved in mammals...
  4. pmc Drosophila P elements preferentially transpose to replication origins
    Allan C Spradling
    Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 108:15948-53. 2011
    ..The advantage transposons gain by moving from replicated to unreplicated genomic regions may contribute to the association of heterochromatin with late-replicating genomic regions...
  5. doi request reprint The living-tissue microscope: the importance of studying stem cells in their natural, undisturbed microenvironment
    Allan C Spradling
    Department of Embryology HHMI, Carnegie Institution, Carnegie Institution, Baltimore, MD 21218, USA
    J Pathol 225:161-2. 2011
    ..Gain-of-function assays such as transplantation are useful, but are not equivalent to studying cells in their natural, undisturbed microenvironment...
  6. pmc Learning the common language of genetics
    Allan C Spradling
    Department of Embryology, Carnegie Institution of Washington HHMI, Baltimore, MD 21218, USA
    Genetics 174:1-3. 2006
  7. pmc New roles for model genetic organisms in understanding and treating human disease: report from the 2006 Genetics Society of America meeting
    Allan Spradling
    Department of Embryology HHMI, Carnegie Institution of Washington, Baltimore, MD 21218, USA
    Genetics 172:2025-32. 2006
    ....
  8. pmc Regulation of epithelial stem cell replacement and follicle formation in the Drosophila ovary
    Todd Nystul
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genetics 184:503-15. 2010
    ..These studies provide new insight into the mechanisms that underlie stem cell replacement and follicle formation during Drosophila oogenesis...
  9. ncbi request reprint Multipotent Drosophila intestinal stem cells specify daughter cell fates by differential notch signaling
    Benjamin Ohlstein
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Science 315:988-92. 2007
    ..Thus, ISCs control daughter cell fate by modulating Notch signaling over time. Our studies suggest that ISCs actively coordinate cell production with local tissue requirements by this mechanism...
  10. ncbi request reprint Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries
    Toshie Kai
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, 115 W University Parkway, Baltimore, Maryland 21210, USA
    Nature 428:564-9. 2004
    ..More generally, Drosophila cystocytes now provide a system for studying de-differentiation and its potential as a source of functional stem cells...
  11. ncbi request reprint The stem cell niche: theme and variations
    Benjamin Ohlstein
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, 115 W University Pkwy, Baltimore, Maryland 21210, USA
    Curr Opin Cell Biol 16:693-9. 2004
    ..While niches are an ancient evolutionary device with conserved features across diverse organisms, we suggest that certain niches display important differences in their organization and function...
  12. pmc Germline stem cells
    Allan Spradling
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution, Baltimore, Maryland 21218, USA
    Cold Spring Harb Perspect Biol 3:a002642. 2011
    ..Despite these apparent differences, GSCs in all organisms share many common mechanisms, although not necessarily molecules, to guarantee survival of the germline...
  13. pmc An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells
    Toshie Kai
    Department of Embryology, The Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Proc Natl Acad Sci U S A 100:4633-8. 2003
    ..Thus, empty niches can persist, signal incoming cells, and support ectopic proliferation. Similar events may underlie some disease states...
  14. ncbi request reprint An epithelial niche in the Drosophila ovary undergoes long-range stem cell replacement
    Todd Nystul
    Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Cell Stem Cell 1:277-85. 2007
    ..The ability of stem cell daughters to target a distant niche and displace its resident stem cell suggests that precancerous mutations might spread from niche to niche within stem cell-based tissues...
  15. ncbi request reprint The adult Drosophila posterior midgut is maintained by pluripotent stem cells
    Benjamin Ohlstein
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, Maryland 21218, USA
    Nature 439:470-4. 2006
    ..The identification of Drosophila intestinal stem cells with striking similarities to their vertebrate counterparts will facilitate the genetic analysis of normal and abnormal intestinal function...
  16. doi request reprint Drosophila stem cell niches: a decade of discovery suggests a unified view of stem cell regulation
    Vicki P Losick
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution for Science, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Dev Cell 21:159-71. 2011
    ..We review the five best-understood adult Drosophila stem cells and argue that the fundamental biology of stem cells and niches is conserved between Drosophila and mice...
  17. ncbi request reprint Regulation of chromatin structure and gene activity by poly(ADP-ribose) polymerases
    Alexei Tulin
    HHMI Laboratories, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    Curr Top Dev Biol 56:55-83. 2003
  18. pmc Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny
    Michael Buszczak
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution, Baltimore, MD 21218, USA
    Science 323:248-51. 2009
    ..Our findings suggest that inhibiting H2B ubiquitylation through scny represents a common mechanism within stem cells that is used to repress the premature expression of key differentiation genes, including Notch target genes...
  19. pmc Epigenetic stability increases extensively during Drosophila follicle stem cell differentiation
    Andrew D Skora
    Department of Embryology, Howard Hughes Medical Institute Research Laboratories, Carnegie Institution, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 107:7389-94. 2010
    ..Modulating epigenetic inheritance may be a critical process controlling transitions between the pleuripotent and differentiated states...
  20. ncbi request reprint The Drosophila ovarian and testis stem cell niches: similar somatic stem cells and signals
    Eva Decotto
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA
    Dev Cell 9:501-10. 2005
    ..These observations reveal striking similarities in the stem cell niches of male and female gonads, and suggest that they are largely governed by common mechanisms...
  21. ncbi request reprint Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci
    Alexei Tulin
    Howard Hughes Medical Research Laboratories, Embryology Department, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Science 299:560-2. 2003
    ..Such local loosening may facilitate transcription and may transiently make protein complexes more accessible to modification, promoting chromatin remodeling during development...
  22. ncbi request reprint The expression profile of purified Drosophila germline stem cells
    Toshie Kai
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution, Baltimore, MD 21210, USA
    Dev Biol 283:486-502. 2005
    ..Our findings suggest that Drosophila and mammalian stem cells utilize at least two regulatory mechanisms in common...
  23. ncbi request reprint Milton controls the early acquisition of mitochondria by Drosophila oocytes
    Rachel T Cox
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Development 133:3371-7. 2006
    ....
  24. ncbi request reprint A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis
    Rachel T Cox
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, 115 W University Parkway, Baltimore, MD 21210, USA
    Development 130:1579-90. 2003
    ..Our findings reveal new similarities between oogenesis in Drosophila and vertebrates, and support our hypothesis that developing oocytes contain specific mechanisms to ensure that germ plasm is endowed with highly functional organelles...
  25. pmc Clueless, a conserved Drosophila gene required for mitochondrial subcellular localization, interacts genetically with parkin
    Rachel T Cox
    Department of Embryology Howard Hughes Medical Institute, Carnegie Institution, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Dis Model Mech 2:490-9. 2009
    ..Disruption of the Clu pathway may enhance oxidative damage, alter gene expression, cause mitochondria to cluster at microtubule plus ends, and lead eventually to mitochondrial failure...
  26. ncbi request reprint Breaking out of the mold: diversity within adult stem cells and their niches
    Todd G Nystul
    Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Curr Opin Genet Dev 16:463-8. 2006
    ..Understanding how stem cells and their progeny are controlled will illuminate fundamental biological mechanisms that govern the construction and maintenance of tissues within metazoan animals...
  27. pmc Drosophila poly(ADP-ribose) glycohydrolase mediates chromatin structure and SIR2-dependent silencing
    Alexei Tulin
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genetics 172:363-71. 2006
    ..In the absence of PARG, SIR2 is mislocalized and hypermodified. We propose that PARP and PARG promote chromatin silencing at least in part by regulating the localization and function of SIR2 and possibly other nuclear proteins...
  28. pmc New components of the Drosophila fusome suggest it plays novel roles in signaling and transport
    Daniel V Lighthouse
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution, Baltimore, MD 21218, USA
    Dev Biol 317:59-71. 2008
    ..In contrast, rab11 is required to maintain the germline stem cells, and to maintain the vesicle content of the spectrosome, suggesting that the fusome mediates intercellular signals that depend on the recycling endosome...
  29. ncbi request reprint Searching chromatin for stem cell identity
    Michael Buszczak
    Howard Hughes Laboratories and Embryology Department, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Cell 125:233-6. 2006
    ..Understanding how these configurations are stabilized by Polycomb group proteins will advance our understanding of embryonic development, tissue homeostasis, regeneration, aging, and oncogenesis...
  30. ncbi request reprint Alpha-endosulfine, a potential regulator of insulin secretion, is required for adult tissue growth control in Drosophila
    Daniela Drummond-Barbosa
    Carnegie Institution of Washington, Howard Hughes Medical Institute Department of Embryology, Baltimore, MD 21210, USA
    Dev Biol 266:310-21. 2004
    ....
  31. ncbi request reprint The Sf1-related nuclear hormone receptor Hr39 regulates Drosophila female reproductive tract development and function
    Anna K Allen
    Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution, 3520 San Martin Drive, Baltimore, MD 21218, USA
    Development 135:311-21. 2008
    ..Our findings suggest that Drosophila can be used to model more aspects of mammalian reproductive biology than previously believed...
  32. ncbi request reprint Male and female Drosophila germline stem cells: two versions of immortality
    Margaret T Fuller
    Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 316:402-4. 2007
    ..Comparing these two stem cells and their niches in detail is likely to reveal how a common heritage has been adapted to the differing requirements of male and female gamete production...
  33. ncbi request reprint Stem cells: more like a man
    Allan C Spradling
    Nature 428:133-4. 2004
  34. doi request reprint Stem cells and niches: mechanisms that promote stem cell maintenance throughout life
    Sean J Morrison
    Howard Hughes Medical Institute, Life Sciences Institute, and Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI, 48109 2216, USA
    Cell 132:598-611. 2008
    ..Mechanisms of stem cell maintenance are key to the regulation of homeostasis and likely contribute to aging and tumorigenesis when altered during adulthood...
  35. pmc The 2003 George W. Beadle Medal; Gerald M. Rubin and Allan C. Spradling
    Terry L Orr-Weaver
    Genetics 164:1248-49. 2003
  36. pmc The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes
    Hugo J Bellen
    Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 167:761-81. 2004
    ..Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool...
  37. pmc The Drosophila heterochromatic gene encoding poly(ADP-ribose) polymerase (PARP) is required to modulate chromatin structure during development
    Alexei Tulin
    Howard Hughes Medical Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    Genes Dev 16:2108-19. 2002
    ..We propose that PARP-e autoregulates Parp transcription by influencing the chromatin structure of its heterochromatic environment. Our results indicate that Parp plays a fundamental role organizing the structure of Drosophila chromatin...

Research Grants3

  1. "Model organisms to human biology: strategies for the future of genetic research"
    Allan Spradling; Fiscal Year: 2007
    ..This meeting will teach participants how to find and exploit the ideal model system in which to study the particular disease or process they are interested in. ..
  2. NORMAL AND MUTANT CHORION GENES OF DROSOPHILA
    Allan Spradling; Fiscal Year: 2003
    ..Therefore, these studies will elucidate the structure and function of eukaryotic chromosomes, a subject of widespread importance to the genetics of all organisms, including humans. ..