J Minden

Summary

Affiliation: Carnegie Mellon University
Country: USA

Publications

  1. ncbi Two-dimensional difference gel electrophoresis
    Jonathan S Minden
    Mellon Institute, Carnegie Mellon University, Pittsburgh, PA, USA
    Methods Mol Biol 869:287-304. 2012
  2. ncbi DIGE: past and future
    Jonathan S Minden
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
    Methods Mol Biol 854:3-8. 2012
  3. ncbi Photoactivated gene expression for cell fate mapping and cell manipulation
    J Minden
    Department of Biological Sciences and Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA, USA
    Sci STKE 2000:pl1. 2000
  4. ncbi Difference gel electrophoresis
    Jonathan S Minden
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Electrophoresis 30:S156-61. 2009
  5. ncbi Two-dimensional difference gel electrophoresis
    Surya Viswanathan
    Department of Biological Science, Carnegie Mellon University, Mellon Institute, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nat Protoc 1:1351-8. 2006
  6. ncbi Caspase-independent cell engulfment mirrors cell death pattern in Drosophila embryos
    Jaime Mergliano
    Department of Biological Sciences and Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Development 130:5779-89. 2003
  7. ncbi Drosophila ventral furrow morphogenesis: a proteomic analysis
    Lei Gong
    Department of Biological Sciences and The NSF Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Development 131:643-56. 2004
  8. ncbi Building proteomic pathways using Drosophila ventral furrow formation as a model
    Mamta Puri
    Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Mol Biosyst 4:1126-35. 2008

Collaborators

  • Mamta Puri
  • Surya Viswanathan
  • Susan R Dowd
  • Lei Gong
  • Jaime Mergliano
  • Nina Senutovich
  • Anupam Goyal
  • Katherine Robertson
  • Margaret Young
  • Arun Krishnaswamy

Detail Information

Publications8

  1. ncbi Two-dimensional difference gel electrophoresis
    Jonathan S Minden
    Mellon Institute, Carnegie Mellon University, Pittsburgh, PA, USA
    Methods Mol Biol 869:287-304. 2012
    ..DIGE combined with digital image analysis therefore greatly improves the statistical assessment of proteome variation. Here we describe a protocol for conducting DIGE experiments, which takes 2-3 days to complete...
  2. ncbi DIGE: past and future
    Jonathan S Minden
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
    Methods Mol Biol 854:3-8. 2012
    ..This chapter provides a brief historical perspective of the development of difference gel electrophoresis, from its inception to commercialization and beyond...
  3. ncbi Photoactivated gene expression for cell fate mapping and cell manipulation
    J Minden
    Department of Biological Sciences and Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA, USA
    Sci STKE 2000:pl1. 2000
    ..The methods for purifying, caging, injection, and photoactivation of the GAL4VP16 protein, and methods for the visualization of marked cells are described in detail...
  4. ncbi Difference gel electrophoresis
    Jonathan S Minden
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Electrophoresis 30:S156-61. 2009
    ..This review highlights some of the improvements and applications of DIGE. We hope these examples are illustrative of what has been done and where the field is headed...
  5. ncbi Two-dimensional difference gel electrophoresis
    Surya Viswanathan
    Department of Biological Science, Carnegie Mellon University, Mellon Institute, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nat Protoc 1:1351-8. 2006
    ..DIGE combined with digital image analysis therefore greatly improves the statistical assessment of proteome variation. Here we describe a protocol for conducting DIGE experiments, which takes 2-3 d to complete...
  6. ncbi Caspase-independent cell engulfment mirrors cell death pattern in Drosophila embryos
    Jaime Mergliano
    Department of Biological Sciences and Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Development 130:5779-89. 2003
    ..Surprisingly, the pattern of cell engulfment persisted in apoptosis-deficient embryos. We provide evidence for a caspase-independent engulfment process that affects the majority of cells expected to die in developing Drosophila embryos...
  7. ncbi Drosophila ventral furrow morphogenesis: a proteomic analysis
    Lei Gong
    Department of Biological Sciences and The NSF Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Development 131:643-56. 2004
    ..RNAi knockdown of these proteasome subunits and time-dependent difference-proteins caused ventral furrow defects, validating the role of these proteins in ventral furrow morphogenesis...
  8. ncbi Building proteomic pathways using Drosophila ventral furrow formation as a model
    Mamta Puri
    Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Mol Biosyst 4:1126-35. 2008
    ..Together these three proteins are part of a regulatory loop that coordinately controls a large number of ventral-specific protein changes...