- The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragmentsNicholas T Ingolia
Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California, USA
Nat Protoc 7:1534-50. 2012..The protocol we describe requires 5-7 days to generate a completed ribosome profiling sequencing library. Sequencing and data analysis require a further 4-5 days...
- Ribosome profiling of mouse embryonic stem cells reveals the complexity and dynamics of mammalian proteomesNicholas T Ingolia
Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
Cell 147:789-802. 2011..We also define a class of short, polycistronic ribosome-associated coding RNAs (sprcRNAs) that encode small proteins. Our studies reveal an unanticipated complexity to mammalian proteomes...
- Many Paths to the Same End: Histone Transcripts Recruit Canonical Initiation Factors through Unconventional InteractionsNicholas Ingolia
Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
Mol Cell 41:133-5. 2011..2011)...