Marcel Bruchez

Summary

Affiliation: Carnegie Mellon University
Country: USA

Publications

  1. pmc Inferring biological structures from super-resolution single molecule images using generative models
    Suvrajit Maji
    Lane Center for Computational Biology, School of Computer Science, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e36973. 2012
  2. pmc Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules
    Saumya Saurabh
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, Pennsylvania 15213, USA
    Opt Express 20:7338-49. 2012
  3. pmc Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
    Rowena Mittal
    Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Bioconjug Chem 22:362-8. 2011
  4. pmc Quantum dots find their stride in single molecule tracking
    Marcel P Bruchez
    Carnegie Mellon University, Department of Chemistry, Pittsburgh, PA 15213, USA
    Curr Opin Chem Biol 15:775-80. 2011
  5. ncbi request reprint Noninvasive imaging of quantum dots in mice
    Byron Ballou
    Molecular Biosensor and Imaging Center, and Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Bioconjug Chem 15:79-86. 2004
  6. ncbi request reprint Fluorogen-activating single-chain antibodies for imaging cell surface proteins
    Christopher Szent-Gyorgyi
    Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nat Biotechnol 26:235-40. 2008
  7. ncbi request reprint Sentinel lymph node imaging using quantum dots in mouse tumor models
    Byron Ballou
    Molecular Biosensor and Imaging Center, Department of Biological Sciences, and Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Bioconjug Chem 18:389-96. 2007
  8. ncbi request reprint Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
    Xingyong Wu
    Quantum Dot Corporation, 26118 Research Rd, Hayward, CA 94545, USA
    Nat Biotechnol 21:41-6. 2003
  9. ncbi request reprint Water-soluble quantum dots for multiphoton fluorescence imaging in vivo
    Daniel R Larson
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Science 300:1434-6. 2003
  10. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006

Collaborators

  • Byron Ballou
  • Christopher Szent Gyorgyi
  • B Christoffer Lagerholm
  • Alan Waggoner
  • Stephen P Perfetto
  • Larry C Mattheakis
  • Xingyong Wu
  • Suvrajit Maji
  • Saumya Saurabh
  • Rowena Mittal
  • Hamid Daneshvar
  • Christopher Szent-Gyorgyi
  • James A J Fitzpatrick
  • Subhasish K Chakraborty
  • Pratip K Chattopadhyay
  • Daniel R Larson
  • Qi Yan
  • Kelly L Zakel
  • Jean Tkach
  • Gregory W Fisher
  • Yehuda Creeger
  • Heather Jackson
  • William Davros
  • Brigitte F Schmidt
  • Todd Peterson
  • Osman Muhammad
  • Peter B Berget
  • Michael A Vogelbaum
  • Sally Adler
  • Carol A Woolford
  • Jonathan W Jarvik
  • Jennifer Nelms
  • Brigitte A Schmidt
  • Steven A Toms
  • Kalin V Vasilev
  • Julie A Phillippi
  • Susan Andreko
  • David A Price
  • Richard A Koup
  • Stephen C De Rosa
  • Paul Goepfert
  • Joanne Yu
  • Mario Roederer
  • Michael R Betts
  • Theresa F Harper
  • Emma Gostick
  • Rebecca M Williams
  • Stephen W Clark
  • Warren R Zipfel
  • Watt W Webb
  • Frank W Wise

Detail Information

Publications14

  1. pmc Inferring biological structures from super-resolution single molecule images using generative models
    Suvrajit Maji
    Lane Center for Computational Biology, School of Computer Science, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e36973. 2012
    ..This approach significantly increases the temporal resolution for dynamic imaging and provides quantitatively useful biological information...
  2. pmc Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules
    Saumya Saurabh
    Department of Chemistry, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, Pennsylvania 15213, USA
    Opt Express 20:7338-49. 2012
    ..Our findings indicate that the sCMOS cameras perform similar to EMCCD cameras for detecting and localizing single Cy5 molecules...
  3. pmc Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
    Rowena Mittal
    Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Bioconjug Chem 22:362-8. 2011
    ..Results showed that 5- to 30-fold more biotin binding sites are available on ITK SAv QDs compared to PEG SAv QDs of the same color with no systematic variation of biotin binding capacity with size...
  4. pmc Quantum dots find their stride in single molecule tracking
    Marcel P Bruchez
    Carnegie Mellon University, Department of Chemistry, Pittsburgh, PA 15213, USA
    Curr Opin Chem Biol 15:775-80. 2011
    ..New approaches are emerging where the quantum dots are used as 'hard-sphere' probes for intracellular compartments. Innovations in quantum dot surface modification are poised to substantially expand the utility of these materials...
  5. ncbi request reprint Noninvasive imaging of quantum dots in mice
    Byron Ballou
    Molecular Biosensor and Imaging Center, and Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Bioconjug Chem 15:79-86. 2004
    ..Surface coatings also determined the in vivo localization of the quantum dots. Long-term experiments demonstrated that these quantum dots remain fluorescent after at least four months in vivo...
  6. ncbi request reprint Fluorogen-activating single-chain antibodies for imaging cell surface proteins
    Christopher Szent-Gyorgyi
    Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nat Biotechnol 26:235-40. 2008
    ..The FAP technique is extensible to a wide variety of nonfluorescent dyes...
  7. ncbi request reprint Sentinel lymph node imaging using quantum dots in mouse tumor models
    Byron Ballou
    Molecular Biosensor and Imaging Center, Department of Biological Sciences, and Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Bioconjug Chem 18:389-96. 2007
    ..Examination of the sentinel nodes identified by quantum dot localization showed that at least some contained metastatic tumor foci...
  8. ncbi request reprint Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
    Xingyong Wu
    Quantum Dot Corporation, 26118 Research Rd, Hayward, CA 94545, USA
    Nat Biotechnol 21:41-6. 2003
    ..The results indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection...
  9. ncbi request reprint Water-soluble quantum dots for multiphoton fluorescence imaging in vivo
    Daniel R Larson
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Science 300:1434-6. 2003
    ..We visualized quantum dots dynamically through the skin of living mice, in capillaries hundreds of micrometers deep. We found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales...
  10. ncbi request reprint Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
    Pratip K Chattopadhyay
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892, USA
    Nat Med 12:972-7. 2006
    ....
  11. doi request reprint Imaging characteristics of zinc sulfide shell, cadmium telluride core quantum dots
    Hamid Daneshvar
    Department of Neurosurgery, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
    Nanomedicine (Lond) 3:21-9. 2008
    ..Their electron-dense, metallic cores suggest utility in other clinical imaging modalities...
  12. ncbi request reprint Labeling cellular targets with semiconductor quantum dot conjugates
    Xingyong Wu
    Quantum Dot Corporation, Hayward, California 94545, USA
    Methods Cell Biol 75:171-83. 2004
  13. ncbi request reprint Optical coding of mammalian cells using semiconductor quantum dots
    Larry C Mattheakis
    Quantum Dot Corp, 26118 Research Road, Hayward, CA 94545, USA
    Anal Biochem 327:200-8. 2004
    ....
  14. ncbi request reprint Cholera toxin B conjugated quantum dots for live cell labeling
    Subhasish K Chakraborty
    Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
    Nano Lett 7:2618-26. 2007
    ..Thus CTB conjugates are a practical alternative to polyarginine conjugates for the general labeling of mammalian cells...

Research Grants3

  1. SENSITIVE, MULTIPLEXED ANALYSIS OF BREAST CANCER MARKERS
    Marcel Bruchez; Fiscal Year: 2002
    ..The technology is also directly applicable to many different kinds of tumor analysis and could be used on tissue microarrays to vastly increase the throughput and aid the archiving of tissue section analysis. ..
  2. Bright Blinking Probes for Fast Multicolor Superresolution Imaging in Live Cells
    Marcel Bruchez; Fiscal Year: 2009
    ..abstract_text> ..
  3. Bright Blinking Probes for Fast Multicolor Superresolution Imaging in Live Cells
    Marcel P Bruchez; Fiscal Year: 2010
    ..abstract_text> ..