Eric T Ahrens

Summary

Affiliation: Carnegie Mellon University
Country: USA

Publications

  1. doi request reprint In vivo MRI cell tracking using perfluorocarbon probes and fluorine-19 detection
    Eric T Ahrens
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    NMR Biomed 26:860-71. 2013
  2. doi request reprint Rapid quantification of inflammation in tissue samples using perfluorocarbon emulsion and fluorine-19 nuclear magnetic resonance
    Eric T Ahrens
    Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Biotechniques 50:229-34. 2011
  3. doi request reprint Self-delivering nanoemulsions for dual fluorine-19 MRI and fluorescence detection
    Jelena M Janjic
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, Pennsylvania 15213, USA
    J Am Chem Soc 130:2832-41. 2008
  4. pmc Accelerated fluorine-19 MRI cell tracking using compressed sensing
    Jia Zhong
    Department of Biological Sciences and the Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Magn Reson Med 69:1683-90. 2013
  5. doi request reprint Functional assessment of human dendritic cells labeled for in vivo (19)F magnetic resonance imaging cell tracking
    Brooke M Helfer
    Department of Research and Development, Celsense Inc, Pittsburgh, Pennsylvania, USA
    Cytotherapy 12:238-50. 2010
  6. pmc Gene expression analysis of dendritic cells that prevent diabetes in NOD mice: analysis of chemokines and costimulatory molecules
    Penelope A Morel
    Department of Immunology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Leukoc Biol 90:539-50. 2011
  7. pmc Sensitive and automated detection of iron-oxide-labeled cells using phase image cross-correlation analysis
    Parker H Mills
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Magn Reson Imaging 26:618-28. 2008
  8. pmc Automated detection and characterization of SPIO-labeled cells and capsules using magnetic field perturbations
    Parker H Mills
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Magn Reson Med 67:278-89. 2012
  9. pmc Assaying macrophage activity in a murine model of inflammatory bowel disease using fluorine-19 MRI
    Deepak K Kadayakkara
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Lab Invest 92:636-45. 2012
  10. pmc 19F MRI detection of acute allograft rejection with in vivo perfluorocarbon labeling of immune cells
    T Kevin Hitchens
    Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Magn Reson Med 65:1144-53. 2011

Collaborators

Detail Information

Publications25

  1. doi request reprint In vivo MRI cell tracking using perfluorocarbon probes and fluorine-19 detection
    Eric T Ahrens
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    NMR Biomed 26:860-71. 2013
    ..We also address the potential applicability of (19) F cell tracking to clinical trials...
  2. doi request reprint Rapid quantification of inflammation in tissue samples using perfluorocarbon emulsion and fluorine-19 nuclear magnetic resonance
    Eric T Ahrens
    Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Biotechniques 50:229-34. 2011
    ..The IFI linearly correlated to mRNA levels of CD68 via real-time PCR analysis. This (19)F NMR approach can accelerate tissue analysis by at least an order of magnitude compared with histological approaches...
  3. doi request reprint Self-delivering nanoemulsions for dual fluorine-19 MRI and fluorescence detection
    Jelena M Janjic
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, Pennsylvania 15213, USA
    J Am Chem Soc 130:2832-41. 2008
    ..The intracellular fluorescence signal is directly proportional to the 19F NMR signal and can be used to calibrate cell loading in vitro...
  4. pmc Accelerated fluorine-19 MRI cell tracking using compressed sensing
    Jia Zhong
    Department of Biological Sciences and the Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Magn Reson Med 69:1683-90. 2013
    ..Our results demonstrate that three-dimensional compressed sensing methods have potential for advancing in vivo 19F cell tracking for a wide range of preclinical and translational applications...
  5. doi request reprint Functional assessment of human dendritic cells labeled for in vivo (19)F magnetic resonance imaging cell tracking
    Brooke M Helfer
    Department of Research and Development, Celsense Inc, Pittsburgh, Pennsylvania, USA
    Cytotherapy 12:238-50. 2010
    ..We provide an assessment of this technology and examine its impact on the health and function of the DC...
  6. pmc Gene expression analysis of dendritic cells that prevent diabetes in NOD mice: analysis of chemokines and costimulatory molecules
    Penelope A Morel
    Department of Immunology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Leukoc Biol 90:539-50. 2011
    ..These results suggest that the therapeutic function of DCs is mediated by a combination of costimulatory and chemokine properties that results in the attraction of diabetogenic Th1 and the induction of Th2 and/or Treg differentiation...
  7. pmc Sensitive and automated detection of iron-oxide-labeled cells using phase image cross-correlation analysis
    Parker H Mills
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Magn Reson Imaging 26:618-28. 2008
    ..In the heart, this information was used to calculate a quantitative index that may indicate its degree of cellular infiltration...
  8. pmc Automated detection and characterization of SPIO-labeled cells and capsules using magnetic field perturbations
    Parker H Mills
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Magn Reson Med 67:278-89. 2012
    ..PDQ can be used with conventional gradient-echo pulse sequences, requiring no extra scan time. The method is useful for visualizing biodistribution of cells and theranostic magnetocapsules and for measuring their relative iron content...
  9. pmc Assaying macrophage activity in a murine model of inflammatory bowel disease using fluorine-19 MRI
    Deepak K Kadayakkara
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Lab Invest 92:636-45. 2012
    ..Due to its high macrophage specificity and quantitative ability, we envisage (19)F MRI having an important role in evaluating a wide range of chronic inflammatory conditions mediated by macrophages...
  10. pmc 19F MRI detection of acute allograft rejection with in vivo perfluorocarbon labeling of immune cells
    T Kevin Hitchens
    Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA
    Magn Reson Med 65:1144-53. 2011
    ..With no background signal, (19) F MRI/magnetic resonance spectroscopy can provide unambiguous detection of fluorine labeled cells, and may be a useful technique for detecting and quantifying rejection grade in patients...
  11. pmc In vivo observation of intracellular oximetry in perfluorocarbon-labeled glioma cells and chemotherapeutic response in the CNS using fluorine-19 MRI
    Deepak K K Kadayakkara
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Magn Reson Med 64:1252-9. 2010
    ..Overall, noninvasive (19) F magnetic resonance techniques may provide a valuable preclinical tool for monitoring therapeutic response against central nervous system or other deep-seated tumors...
  12. pmc In vivo intracellular oxygen dynamics in murine brain glioma and immunotherapeutic response of cytotoxic T cells observed by fluorine-19 magnetic resonance imaging
    Jia Zhong
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e59479. 2013
    ..These results indicate the high sensitivity of this approach and its utility as a non-invasive surrogate biomarker of anti-cancer immunotherapeutic response in preclinical models...
  13. pmc Inflammation driven by overexpression of the hypoglycosylated abnormal mucin 1 (MUC1) links inflammatory bowel disease and pancreatitis
    Deepak K Kadayakkara
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
    Pancreas 39:510-5. 2010
    ..MUC1 is also expressed on pancreatic ductal epithelia. We tested the possibility that in IBD, MUC1 expression on pancreatic ducts is also abnormal leading to inflammation and pancreatitis...
  14. pmc Fluorine-containing nanoemulsions for MRI cell tracking
    Jelena M Janjic
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Wiley Interdiscip Rev Nanomed Nanobiotechnol 1:492-501. 2009
    ..This technology can be widely applied to studies of inflammation, cellular regenerative medicine, and immunotherapy...
  15. ncbi request reprint Fluorine-19 MRI for visualization and quantification of cell migration in a diabetes model
    Mangala Srinivas
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Magn Reson Med 58:725-34. 2007
    ..The labeling procedure did not affect T-cell migration in vivo. This imaging platform is applicable to many cell types and disease models and can potentially be used for monitoring the trafficking of cellular therapeutics...
  16. pmc Enhanced positive-contrast visualization of paramagnetic contrast agents using phase images
    Parker H Mills
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Magn Reson Med 62:1349-55. 2009
    ..This approach can be used in conjunction with conventional T(2)* pulse sequences, requires no prescans or increased scan time, and can be applied retrospectively to previously acquired data...
  17. pmc Engineered mitochondrial ferritin as a magnetic resonance imaging reporter in mouse olfactory epithelium
    Bistra Iordanova
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e72720. 2013
    ..This robust MRI reporter can facilitate the study of the molecular mechanisms of olfaction and to monitor intranasal gene therapy delivery, as well as a wide range of cell tracking and gene expression studies in living subjects. ..
  18. pmc In vivo magnetic resonance imaging of ferritin-based reporter visualizes native neuroblast migration
    Bistra Iordanova
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA
    Neuroimage 59:1004-12. 2012
    ..This MRI reporter gene platform can facilitate the non-invasive study of native or transplanted stem cell migration and associated neurogenic or therapeutic molecular events in live animals...
  19. pmc Visualizing arthritic inflammation and therapeutic response by fluorine-19 magnetic resonance imaging (19F MRI)
    Anthony Balducci
    Department of Research and Development, Celsense, Inc, Pittsburgh, PA 15222, USA
    J Inflamm (Lond) 9:24. 2012
    ..abstract:..
  20. ncbi request reprint Theoretical MRI contrast model for exogenous T2 agents
    Parker H Mills
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Magn Reson Med 57:442-7. 2007
    ..In the Appendix we present a numerical formalism that is useful for relating image contrast and agent concentration when gradient-echo (GRE) T2*-weighted (T2*W) pulse sequences are used...
  21. ncbi request reprint In vivo imaging of autoimmune disease in model systems
    Eric T Ahrens
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    Curr Top Dev Biol 70:215-38. 2005
    ..This is an area of intense recent research and can be expanded to other conditions such as cancer...
  22. ncbi request reprint In vivo imaging platform for tracking immunotherapeutic cells
    Eric T Ahrens
    Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, 4400 Fifth Ave, Pittsburgh, Pennsylvania 15213, USA
    Nat Biotechnol 23:983-7. 2005
    ..Although we focused on DCs, which are being developed as immunotherapeutics for cancer and autoimmune diseases, this technology should be useful for monitoring a wide range of cell types in vivo...
  23. doi request reprint Intracellular pH Measurements Using Perfluorocarbon Nanoemulsions
    Michael J Patrick
    Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States
    J Am Chem Soc 135:18445-57. 2013
    ..The pH-sensing nanoemulsions allow the study of the fate of the PFC tracer inside the labeled cell, which is important for understanding the PFC cell loading dynamics, nanoemulsion stability and cell viability over time. ..
  24. pmc Design and characterization of a chimeric ferritin with enhanced iron loading and transverse NMR relaxation rate
    Bistra Iordanova
    Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    J Biol Inorg Chem 15:957-65. 2010
    ..Interestingly, we found that the L*H chimera exhibits a significantly enhanced iron loading ability and T (2) relaxation compared to wild-type ferritin. We suggest that the L*H chimera may be useful as a sensitive MRI reporter molecule...
  25. pmc Semiquantitative histopathology and 3D magnetic resonance microscopy as collaborative platforms for tissue identification and comparison within teratomas derived from pedigreed primate embryonic stem cells
    Carlos A Castro
    Pittsburgh Development Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Stem Cell Res 5:201-11. 2010
    ..MRM provides rapid quantitative imaging of intact teratomas that complements histology and identifies sites of interest for additional biological studies...