Tatjana Sauka-Spengler

Summary

Affiliation: California Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Insights from a sea lamprey into the evolution of neural crest gene regulatory network
    Tatjana Sauka-Spengler
    Division of Biology 139 74, California Institute of Technology, Pasadena, California 91125, USA
    Biol Bull 214:303-14. 2008
  2. doi request reprint A gene regulatory network orchestrates neural crest formation
    Tatjana Sauka-Spengler
    Division of Biology 13974, California Institute of Technology, Pasadena, California 91125, USA
    Nat Rev Mol Cell Biol 9:557-68. 2008
  3. pmc Ancient Pbx-Hox signatures define hundreds of vertebrate developmental enhancers
    Hugo J Parker
    Division of Systems Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    BMC Genomics 12:637. 2011
  4. doi request reprint Whole-mount in situ hybridization on lamprey embryos
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.prot5125. 2009
  5. doi request reprint The sea lamprey Petromyzon marinus: a model for evolutionary and developmental biology
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.emo113. 2009
  6. pmc Expression of sympathetic nervous system genes in Lamprey suggests their recruitment for specification of a new vertebrate feature
    Daniela Häming
    Division of Biology, California Institute of Technology, Pasadena, California, United States of America
    PLoS ONE 6:e26543. 2011
  7. pmc Histone demethylase JmjD2A regulates neural crest specification
    Pablo Hernan Strobl-Mazzulla
    Division of Biology 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Cell 19:460-8. 2010
  8. pmc Dynamic and differential regulation of stem cell factor FoxD3 in the neural crest is Encrypted in the genome
    Marcos S Simões-Costa
    Division of Biology, California Institute of Technology, Pasadena, California, USA
    PLoS Genet 8:e1003142. 2012
  9. doi request reprint Expression of Sox family genes in early lamprey development
    Benjamin R Uy
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Int J Dev Biol 56:377-83. 2012
  10. doi request reprint Immunostaining of whole-mount and sectioned lamprey embryos
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.prot5126. 2009

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Insights from a sea lamprey into the evolution of neural crest gene regulatory network
    Tatjana Sauka-Spengler
    Division of Biology 139 74, California Institute of Technology, Pasadena, California 91125, USA
    Biol Bull 214:303-14. 2008
    ..In contrast, certain differences were observed in deployment of some neural crest specifier and downstream effector genes expected to confer species-specific migratory and differentiation properties...
  2. doi request reprint A gene regulatory network orchestrates neural crest formation
    Tatjana Sauka-Spengler
    Division of Biology 13974, California Institute of Technology, Pasadena, California 91125, USA
    Nat Rev Mol Cell Biol 9:557-68. 2008
    ..Here, we review our current understanding of these processes and discuss the molecular players that are involved in the neural crest gene regulatory network...
  3. pmc Ancient Pbx-Hox signatures define hundreds of vertebrate developmental enhancers
    Hugo J Parker
    Division of Systems Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    BMC Genomics 12:637. 2011
    ..Whilst comparative genomics approaches have identified thousands of putative regulatory elements, our knowledge of their mechanism of action is poor and very little progress has been made in systematically de-coding them...
  4. doi request reprint Whole-mount in situ hybridization on lamprey embryos
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.prot5125. 2009
    ..In addition, studies of features that are unique to lampreys can provide insights into mechanisms of parallel evolution. This protocol describes an optimized procedure for RNA in situ hybridization in lamprey embryos...
  5. doi request reprint The sea lamprey Petromyzon marinus: a model for evolutionary and developmental biology
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.emo113. 2009
    ..marinus is poised to become an evolutionary developmental model of choice...
  6. pmc Expression of sympathetic nervous system genes in Lamprey suggests their recruitment for specification of a new vertebrate feature
    Daniela Häming
    Division of Biology, California Institute of Technology, Pasadena, California, United States of America
    PLoS ONE 6:e26543. 2011
    ..This raises the intriguing possibility that they may have been recruited during gnathostome evolution to a new function in a neural crest derivative...
  7. pmc Histone demethylase JmjD2A regulates neural crest specification
    Pablo Hernan Strobl-Mazzulla
    Division of Biology 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Cell 19:460-8. 2010
    ..Our findings show that chromatin modifications directly control neural crest genes in vertebrate embryos via modulating histone methylation...
  8. pmc Dynamic and differential regulation of stem cell factor FoxD3 in the neural crest is Encrypted in the genome
    Marcos S Simões-Costa
    Division of Biology, California Institute of Technology, Pasadena, California, USA
    PLoS Genet 8:e1003142. 2012
    ....
  9. doi request reprint Expression of Sox family genes in early lamprey development
    Benjamin R Uy
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Int J Dev Biol 56:377-83. 2012
    ..Coupled with phylogenetic analysis of these Sox genes, the results provide insight into gene duplication and di-vergence in paralog deployment occurring during early vertebrate evolution...
  10. doi request reprint Immunostaining of whole-mount and sectioned lamprey embryos
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.prot5126. 2009
    ..This protocol describes how to immunostain whole-mount or sectioned lamprey embryos using an antibody raised against the protein of interest and detected with a horseradish peroxidase (HRP)-conjugated secondary antibody...
  11. doi request reprint Assembling neural crest regulatory circuits into a gene regulatory network
    Paola Betancur
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Annu Rev Cell Dev Biol 26:581-603. 2010
    ....
  12. doi request reprint Chapter 1. Gene regulatory networks in neural crest development and evolution
    Natalya Nikitina
    Divison of Biology, California Institute of Technology, Pasadena, CA, USA
    Curr Top Dev Biol 86:1-14. 2009
    ..We summarize recent evidence for the ongoing diversification of the neural crest in several vertebrate species, with particular reference to studies in nontraditional vertebrate model organisms...
  13. doi request reprint DiI cell labeling in lamprey embryos
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.prot5124. 2009
    ..This protocol describes how to label lamprey embryo cells by microinjecting the fluorescent dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) to study cell fate during development...
  14. pmc DNA methyltransferase3A as a molecular switch mediating the neural tube-to-neural crest fate transition
    Na Hu
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Genes Dev 26:2380-5. 2012
    ..Thus, DNMT3A functions as a molecular switch, repressing neural to favor neural crest cell fate...
  15. pmc A Sox10 enhancer element common to the otic placode and neural crest is activated by tissue-specific paralogs
    Paola Betancur
    Division of Biology 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Development 138:3689-98. 2011
    ....
  16. doi request reprint Microinjection of RNA and morpholino oligos into lamprey embryos
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.prot5123. 2009
    ..Finally, the second division lasts for several hours, providing a very large injection window. This protocol describes how to microinject RNA and morpholinos into lamprey embryos for genetic modification studies...
  17. doi request reprint Culturing lamprey embryos
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cold Spring Harb Protoc 2009:pdb.prot5122. 2009
    ..This protocol describes how to produce lamprey embryos by collecting sperm and eggs from mature lampreys, performing fertilization, and culturing the embryos through to the desired developmental stage...
  18. pmc Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest
    Paola Betancur
    Division of Biology 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 107:3570-5. 2010
    ..Using cis-regulatory analysis as a tool, our study makes critical connections within the neural crest gene regulatory network, thus being unique in establishing a direct link of upstream effectors to a key neural crest specifier...
  19. ncbi request reprint Ancient evolutionary origin of the neural crest gene regulatory network
    Tatjana Sauka-Spengler
    Division of Biology 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Dev Cell 13:405-20. 2007
    ..By testing the collective expression and function of neural crest genes in a single, basal vertebrate, we reveal the ground state of the NC-GRN and resolve ambiguities between model organisms...
  20. pmc Dissecting early regulatory relationships in the lamprey neural crest gene network
    Natalya Nikitina
    Division of Biology, California Institute of Technology, Pasadena, CA 81125, USA
    Proc Natl Acad Sci U S A 105:20083-8. 2008
    ..These findings provide an ancestral framework for performing comparative tests in higher vertebrates in which network linkages may be more difficult to resolve because of their rapid development...
  21. doi request reprint Evolution of the neural crest viewed from a gene regulatory perspective
    Tatjana Sauka-Spengler
    Division of Biology 139 74, California Institute of Technology, Pasadena, California 91125, USA
    Genesis 46:673-82. 2008
    ..Analyzing neural crest formation from a gene regulatory viewpoint provides insights into both the developmental mechanisms and evolutionary origins of this vertebrate-specific cell type...
  22. ncbi request reprint Development and evolution of the migratory neural crest: a gene regulatory perspective
    Tatjana Sauka-Spengler
    Division of Biology 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Curr Opin Genet Dev 16:360-6. 2006
    ..These in turn regulate other transcription factors termed neural crest-specifiers, which control genes involved in neural crest delamination, the generation of migratory cells and ultimately the acquisition of appropriate fates...
  23. doi request reprint Gain- and loss-of-function approaches in the chick embryo
    Tatjana Sauka-Spengler
    California Institute of Technology, Pasadena, California 91125, USA
    Methods Cell Biol 87:237-56. 2008
    ..We routinely overexpress constructs containing 3-4 kb inserts and coharboring a GFP or RFP reporter whose translation is initiated from an internal ribosomal entry site (IRES), thus allowing easy detection of the electroporated cells...
  24. ncbi request reprint Expression of the polycomb group gene bmi-1 in the early chick embryo
    Paige E Fraser
    Division of Biology, 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Gene Expr Patterns 5:23-7. 2004
    ..Bmi-1 expression was also present in the developing heart primordia and the sensory placodes. The data show that bmi-1 is present in, but not restricted to, tissue containing multipotent precursor cells...