DOUGLAS CHARLES REES

Summary

Affiliation: California Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Conversion of a mechanosensitive channel protein from a membrane-embedded to a water-soluble form by covalent modification with amphiphiles
    Christian F W Becker
    Gryphon Therapeutics, 600 Gateway Blvd, South San Francisco, CA 94080, USA
    J Mol Biol 343:747-58. 2004
  2. pmc Structure of a tetrameric MscL in an expanded intermediate state
    Zhenfeng Liu
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Nature 461:120-4. 2009
  3. pmc A distinct mechanism for the ABC transporter BtuCD-BtuF revealed by the dynamics of complex formation
    Oded Lewinson
    Division of Chemistry and Chemical Engineering, Pasadena, California, USA
    Nat Struct Mol Biol 17:332-8. 2010
  4. ncbi request reprint Structural bioenergetics and energy transduction mechanisms
    D C Rees
    Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    J Mol Biol 293:343-50. 1999
  5. ncbi request reprint Great metalloclusters in enzymology
    Douglas C Rees
    Division of Chemistry and Chemical Engineering 147 75CH, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Annu Rev Biochem 71:221-46. 2002
  6. ncbi request reprint Structural basis of biological nitrogen fixation
    Douglas C Rees
    Division of Chemistry and Chemical Engineering, 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Philos Trans A Math Phys Eng Sci 363:971-84; discussion 1035-40. 2005
  7. ncbi request reprint The interface between the biological and inorganic worlds: iron-sulfur metalloclusters
    Douglas C Rees
    Division of Chemistry and Chemical Engineering 114 96, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 300:929-31. 2003
  8. pmc Some thermodynamic implications for the thermostability of proteins
    D C Rees
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Protein Sci 10:1187-94. 2001
  9. ncbi request reprint Nitrogenase: standing at the crossroads
    D C Rees
    Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering, 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    Curr Opin Chem Biol 4:559-66. 2000
  10. pmc ABC transporters: the power to change
    Douglas C Rees
    Division of Chemistry and Chemical Engineering 114 96, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Nat Rev Mol Cell Biol 10:218-27. 2009

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Conversion of a mechanosensitive channel protein from a membrane-embedded to a water-soluble form by covalent modification with amphiphiles
    Christian F W Becker
    Gryphon Therapeutics, 600 Gateway Blvd, South San Francisco, CA 94080, USA
    J Mol Biol 343:747-58. 2004
    ..Solubilization of membrane proteins by covalent attachment of amphiphiles results in homogeneous particles that may prove useful for crystallization, solution NMR spectroscopy, and electron microscopy...
  2. pmc Structure of a tetrameric MscL in an expanded intermediate state
    Zhenfeng Liu
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Nature 461:120-4. 2009
    ....
  3. pmc A distinct mechanism for the ABC transporter BtuCD-BtuF revealed by the dynamics of complex formation
    Oded Lewinson
    Division of Chemistry and Chemical Engineering, Pasadena, California, USA
    Nat Struct Mol Biol 17:332-8. 2010
    ....
  4. ncbi request reprint Structural bioenergetics and energy transduction mechanisms
    D C Rees
    Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    J Mol Biol 293:343-50. 1999
    ..This is illustrated by examples from the nucleotide switch family of proteins, with emphasis on the nitrogenase system where ATP hydrolysis is coupled to an electron transfer reaction...
  5. ncbi request reprint Great metalloclusters in enzymology
    Douglas C Rees
    Division of Chemistry and Chemical Engineering 147 75CH, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Annu Rev Biochem 71:221-46. 2002
    ..These proteins also provide a window into the union of the biological and inorganic worlds that may have been relevant to the early evolution of biochemical catalysis...
  6. ncbi request reprint Structural basis of biological nitrogen fixation
    Douglas C Rees
    Division of Chemistry and Chemical Engineering, 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Philos Trans A Math Phys Eng Sci 363:971-84; discussion 1035-40. 2005
    ....
  7. ncbi request reprint The interface between the biological and inorganic worlds: iron-sulfur metalloclusters
    Douglas C Rees
    Division of Chemistry and Chemical Engineering 114 96, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 300:929-31. 2003
    ....
  8. pmc Some thermodynamic implications for the thermostability of proteins
    D C Rees
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Protein Sci 10:1187-94. 2001
    ..An estimate of the energetic consequences of thermal expansion suggests that these effects may contribute significantly to the destabilization of the native state of proteins with increasing temperature...
  9. ncbi request reprint Nitrogenase: standing at the crossroads
    D C Rees
    Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering, 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    Curr Opin Chem Biol 4:559-66. 2000
    ....
  10. pmc ABC transporters: the power to change
    Douglas C Rees
    Division of Chemistry and Chemical Engineering 114 96, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Nat Rev Mol Cell Biol 10:218-27. 2009
    ..An important goal is to develop quantitative models that detail the kinetic and molecular mechanisms by which ABC transporters couple the binding and hydrolysis of ATP to substrate translocation...
  11. ncbi request reprint Nitrogenase complexes: multiple docking sites for a nucleotide switch protein
    F Akif Tezcan
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Mail Code 114 96, Pasadena, CA 91125, USA
    Science 309:1377-80. 2005
    ....
  12. ncbi request reprint Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel
    Randal B Bass
    Division of Chemistry and Chemical Engineering, Biochemistry Option, Howard Hughes Medical Institute, Mail Code 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Science 298:1582-7. 2002
    ..Although MscS is likely to be structurally distinct from other ion channels, similarities in gating mechanisms suggest common structural elements...
  13. pmc The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation
    Neena S Kadaba
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, Mail Code 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Science 321:250-3. 2008
    ....
  14. ncbi request reprint In vitro functional characterization of BtuCD-F, the Escherichia coli ABC transporter for vitamin B12 uptake
    Elizabeth L Borths
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Biochemistry 44:16301-9. 2005
    ....
  15. ncbi request reprint Structure of a cofactor-deficient nitrogenase MoFe protein
    Benedikt Schmid
    Division of Chemistry and Chemical Engineering, Mail Code 147 75CH, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 296:352-6. 2002
    ..A predominantly positively charged funnel is revealed; this funnel is of sufficient size to accommodate insertion of the negatively charged cofactor...
  16. pmc The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter
    Elizabeth L Borths
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, Mail Code 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 99:16642-7. 2002
    ..These glutamate and arginine residues are conserved among binding proteins and ABC transporters mediating iron and B12 uptake, suggesting that they may have a role in docking and the transmission of conformational changes...
  17. doi request reprint Biochemistry. Opening the molecular floodgates
    Chris S Gandhi
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Science 321:1166-7. 2008
  18. ncbi request reprint Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site
    Tina M Iverson
    Division of Chemistry and Chemical Engineering, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 277:16124-30. 2002
    ..This acidic residue, Glu-C29, in the E. coli enzyme may act as a proton shuttle from the quinol during enzyme turnover...
  19. ncbi request reprint The structures of BtuCD and MscS and their implications for transporter and channel function
    Randal B Bass
    Division of Chemistry and Chemical Engineering, 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    FEBS Lett 555:111-5. 2003
    ....
  20. pmc A P-type ATPase importer that discriminates between essential and toxic transition metals
    Oded Lewinson
    Division of Chemistry and Chemical Engineering 114 96 and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 106:4677-82. 2009
    ..The substrate selectivity of this importer ensures the high-affinity uptake of essential metals, while avoiding intracellular contamination by their toxic counterparts...
  21. pmc JAMM: a metalloprotease-like zinc site in the proteasome and signalosome
    Xavier I Ambroggio
    Division of Biology, California Institute of Technology, Pasadena, California, USA
    PLoS Biol 2:E2. 2004
    ..The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors...
  22. ncbi request reprint The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism
    Kaspar P Locher
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, Mail Code 147 75CH, California Institute of Technology, Pasadena, CA 91125, USA
    Science 296:1091-8. 2002
    ..A prominent cytoplasmic loop of BtuC forms the contact region with the ATP-binding cassette and appears to represent a conserved motif among the ABC transporters...
  23. pmc The funnel approach to the precrystallization production of membrane proteins
    Oded Lewinson
    Division of Chemistry and Chemical Engineering 114 96, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    J Mol Biol 377:62-73. 2008
    ..Following the initial streamlined screening, we were able to fine-tune and produce 9 of the 36 targets as materials suitable for crystallization or other structural studies...
  24. ncbi request reprint Nitrogenase MoFe-protein at 1.16 A resolution: a central ligand in the FeMo-cofactor
    Oliver Einsle
    Howard Hughes Medical Institute, Division of Chemistry and Chemical Engineering, California Institute of Technology, Mail Code 147 75CH, Pasadena, CA 91125, USA
    Science 297:1696-700. 2002
    ..The presence of a nitrogen atom in the cofactor would have important implications for the mechanism of dinitrogen reduction by nitrogenase...
  25. ncbi request reprint Biochemical and structural characterization of the cross-linked complex of nitrogenase: comparison to the ADP-AlF4(-)-stabilized structure
    Benedikt Schmid
    Division of Chemistry and Chemical Engineering 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Biochemistry 41:15557-65. 2002
    ....
  26. ncbi request reprint High resolution crystal structures of the wild type and Cys-55-->Ser and Cys-59-->Ser variants of the thioredoxin-like [2Fe-2S] ferredoxin from Aquifex aeolicus
    Andrew P Yeh
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    J Biol Chem 277:34499-507. 2002
    ....
  27. pmc A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators
    Zhenfeng Liu
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Protein Sci 19:808-14. 2010
    ..We propose this protein be renamed MLPTv for MarR-like protein from T. volcanium...
  28. pmc Investigation of the biophysical and cell biological properties of ferroportin, a multipass integral membrane protein iron exporter
    Adrian E Rice
    Graduate Option in Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, CA 91125, USA
    J Mol Biol 386:717-32. 2009
    ..The characterizations reported here form a basis upon which models for ferroportin's role in regulating iron homeostasis in health and disease can be interpreted...
  29. pmc Open-state disulfide crosslinking between Mycobacterium tuberculosis mechanosensitive channel subunits
    George Shapovalov
    Division of Biology, California Institute of Technology, Pasadena, 91125, USA
    Biophys J 84:2357-65. 2003
    ..6-6.8 A. Such asymmetric structures must be considered in models of TB-MscL gating...
  30. ncbi request reprint Prokaryotic mechanosensitive channels
    Pavel Strop
    California Institute of Technology, Pasadena, California 91125, USA
    Adv Protein Chem 63:177-209. 2003
  31. ncbi request reprint The alpha-helix and the organization and gating of channels
    Robert H Spencer
    Department of Pharmacology, Merck Research Laboratories, West Point, PA 19486
    Annu Rev Biophys Biomol Struct 31:207-33. 2002
    ....
  32. ncbi request reprint X-ray structure determination of the cytochrome c2: reaction center electron transfer complex from Rhodobacter sphaeroides
    Herbert L Axelrod
    Department of Physics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0319, USA
    J Mol Biol 319:501-15. 2002
    ..This domain, in addition to contributing to the binding, may help steer the unbound proteins toward the right conformation...
  33. pmc How many metals does it take to fix N2? A mechanistic overview of biological nitrogen fixation
    James B Howard
    Department of Biochemistry, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 103:17088-93. 2006
    ..This Perspective provides an overview of biological nitrogen fixation and introduces three contributions to this special feature that address central aspects of the mechanism and assembly of nitrogenase...
  34. pmc Assignment of individual metal redox states in a metalloprotein by crystallographic refinement at multiple X-ray wavelengths
    Oliver Einsle
    Institut fur Mikrobiologie und Genetik, Georg August Universitat Gottingen, 37077 Gottingen, Germany
    J Am Chem Soc 129:2210-1. 2007
  35. ncbi request reprint Structural biology. Breaching the barrier
    Kaspar P Locher
    Institut fur Molekularbiologie und Biophysik, Eidgenossische Technische Hochschule Zurich, Zurich CH 8093, Switzerland
    Science 301:603-4. 2003
  36. ncbi request reprint Structure and mechanism in prokaryotic mechanosensitive channels
    Eduardo Perozo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Curr Opin Struct Biol 13:432-42. 2003
    ..Recent functional, structural and dynamic data offer fresh insights into the molecular basis of gating for these membrane proteins...
  37. pmc Structures of the iron-sulfur flavoproteins from Methanosarcina thermophila and Archaeoglobus fulgidus
    Susana L A Andrade
    Institut fur Mikrobiologie und Genetik, Abt Molekulare Strukturbiologie, Georg August Universitat Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    J Bacteriol 187:3848-54. 2005
    ..This is only possible between different monomers upon oligomerization. Fundamental differences in the surface properties of the two ISF homologs underscore the diversity encountered within this protein family...
  38. ncbi request reprint Mo K- and L-edge X-ray absorption spectroscopic study of the ADP.AlF4--stabilized nitrogenase complex: comparison with MoFe protein in solution and single crystal
    Mary C Corbett
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    J Synchrotron Radiat 12:28-34. 2005
    ..The initial results point to the feasibility of using this technique for the analysis of scattering from the as yet unidentified atom at the center of FeMoco...
  39. ncbi request reprint Advances in protein chemistry. Preface
    Douglas C Rees
    Adv Protein Chem 63:xi-xvi. 2003
  40. ncbi request reprint Functional analysis of detergent-solubilized and membrane-reconstituted ATP-binding cassette transporters
    Bert Poolman
    Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute and Materials Science Centre plus, University of Groningen, The Netherlands
    Methods Enzymol 400:429-59. 2005
    ..Also, we present simple methods, based on substrate binding or translocation, to follow the activity of the protein complexes in detergent-solubilized and/or membrane-reconstituted state(s)...
  41. ncbi request reprint Do electrostatic interactions with positively charged active site groups tighten the transition state for enzymatic phosphoryl transfer?
    Ivana Nikolic-Hughes
    Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA
    J Am Chem Soc 126:11814-9. 2004
    ....

Research Grants18

  1. Biophysical, Structural and Functional Analysis of Mechanosensitive Channels
    Douglas Rees; Fiscal Year: 2009
    ..PUBLIC HEALTH RELEVANCE Force-sensing is a critical aspect of healthy cell growth, morphology and development. We will study in molecular detail how force-sensing is achieved by two families of stretch-activated membrane channels. ..
  2. Crystallographic Studies of Electron Tranfer Proteins
    Douglas Rees; Fiscal Year: 2007
    ..Broader implications will address nucleotide dependent transduction processes based on the similarities between Fe-protein and other nucleotide switch proteins involved in signal and energy transduction processes. ..
  3. CRYSTALLOGRAPHIC STUDIES OF ELECTRON TRANSFER PROTEINS
    Douglas Rees; Fiscal Year: 2002
    ..4. the correspondence between crystallographically defined metallocluster structures and spectroscopically assigned oxidation states in the Fe-protein and MoFe-protein. ..
  4. CRYSTALLOGRAPHIC STUDIES OF ELECTRON TRANSFER PROTEINS
    Douglas Rees; Fiscal Year: 1993
    ..Crystallization and structure determination of hydroxylamine oxidoreductase from this organism will be attempted...
  5. Biophysical, Structural and Functional Analysis of Mechanosensitive Channels
    DOUGLAS CHARLES REES; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE Force-sensing is a critical aspect of healthy cell growth, morphology and development. We will study in molecular detail how force-sensing is achieved by two families of stretch-activated membrane channels. ..