Research Topics
| Matthew D PetroskiSummaryAffiliation: California Institute of Technology Country: USA Publications
| Collaborators |
Detail Information
Publications
Function and regulation of cullin-RING ubiquitin ligasesMatthew D Petroski
Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA
Nat Rev Mol Cell Biol 6:9-20. 2005..In this review, we focus on the composition, regulation and function of cullin-RING ligases, and describe how these enzymes can be characterized by a set of general principles...
In vitro reconstitution of SCF substrate ubiquitination with purified proteinsMatthew D Petroski
Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
Methods Enzymol 398:143-58. 2005..Based on our experience in reconstituting Sic1 ubiquitination, we suggest some parameters to consider that should be generally applicable to the study of different SCF complexes and other ubiquitin ligases...
Mechanism of lysine 48-linked ubiquitin-chain synthesis by the cullin-RING ubiquitin-ligase complex SCF-Cdc34Matthew D Petroski
Howard Hughes Medical Institute, Division of Biology, 156 29, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
Cell 123:1107-20. 2005..We propose that the acidic loop favorably positions K48 of a substrate-linked ubiquitin to attack SCF bound Cdc34 approximately ubiquitin thioester and thereby enables processive synthesis of K48-linked ubiquitin chains by SCF-Cdc34...
Evaluation of a diffusion-driven mechanism for substrate ubiquitination by the SCF-Cdc34 ubiquitin ligase complexMatthew D Petroski
Howard Hughes Medical Institute, Division of Biology, 156 29, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, USA
Mol Cell 24:523-34. 2006..We propose that interactions between Cdc34 approximately Ub and SCF directly activate ubiquitin transfer within a substrate-SCF-Cdc34 approximately Ub ternary complex...
Redundant degrons ensure the rapid destruction of Sic1 at the G1/S transition of the budding yeast cell cycleMatthew D Petroski
Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
Cell Cycle 2:410-1. 2003
Context of multiubiquitin chain attachment influences the rate of Sic1 degradationMatthew D Petroski
Howard Hughes Medical Institute, Division of Biology 156 29, California Institute of Technology, 1200 E California Boulevard, Pasadena, CA 91125, USA
Mol Cell 11:1435-44. 2003..Our results reveal that a single multiubiquitin chain can sustain a physiological turnover rate, but that chain position plays an unexpectedly significant role in the rate of proteasomal proteolysis...
Substrate modification with lysine 63-linked ubiquitin chains through the UBC13-UEV1A ubiquitin-conjugating enzymeMatthew D Petroski
Rigel Pharmaceuticals, Inc, South San Francisco, California 94080, USA
J Biol Chem 282:29936-45. 2007....
