Affiliation: California Institute of Technology
Lin D, Hoelz A. The Structure of the Nuclear Pore Complex (An Update). Annu Rev Biochem. 2019;: pubmed publisher
..Expected final online publication date for the Annual Review of Biochemistry Volume 88 is June 20, 2019. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates. ..
Davenport A, Huber F, Hoelz A. Structural and functional analysis of human SIRT1. J Mol Biol. 2014;426:526-41 pubmed publisher
..A biochemical analysis identifies key residues in the active site, an inhibitory role for the CTR, and distinct structural features of the CTR that mediate binding and inhibition of the SIRT1 catalytic domain. ..
Stuwe T, Correia A, Lin D, Paduch M, Lu V, Kossiakoff A, et al
. Nuclear pores. Architecture of the nuclear pore complex coat. Science. 2015;347:1148-52 pubmed publisher
..Moreover, 32 copies of the CNC crystal structure docked readily into a cryoelectron tomographic reconstruction of the fully assembled human NPC, thereby accounting for ~16 megadalton of its mass. ..
Stuwe T, Bley C, Thierbach K, Petrovic S, Schilbach S, Mayo D, et al
. Architecture of the fungal nuclear pore inner ring complex. Science. 2015;350:56-64 pubmed publisher
..We propose that the IRC adopts a relatively rigid scaffold that recruits the CNT to primarily form the diffusion barrier of the NPC, rather than enabling channel dilation. ..
Lin D, Correia A, Cai S, Huber F, Jette C, Hoelz A. Structural and functional analysis of mRNA export regulation by the nuclear pore complex. Nat Commun. 2018;9:2319 pubmed publisher
..Together, our results provide the foundation for further mechanistic analyses of mRNA export in humans. ..
Lin D, Stuwe T, Schilbach S, Rundlet E, Perriches T, Mobbs G, et al
. Architecture of the symmetric core of the nuclear pore. Science. 2016;352:aaf1015 pubmed publisher
..Our approach provides a paradigm for the structure determination of similarly complex macromolecular assemblies. ..
Hoelz A, Debler E, Blobel G. The structure of the nuclear pore complex. Annu Rev Biochem. 2011;80:613-43 pubmed publisher
..The applied structure determination as well as the described design principles of the NPC may serve as paradigms for other macromolecular assemblies...
Davenport A, Collins L, Chiu H, Minor P, Sternberg P, Hoelz A. Structural and functional characterization of the α-tubulin acetyltransferase MEC-17. J Mol Biol. 2014;426:2605-16 pubmed publisher
..An analysis of MEC-17 mutants in Caenorhabditis elegans shows that enzymatic activity is dispensable for touch sensitivity. ..
Stelter P, Huber F, Kunze R, Flemming D, Hoelz A, Hurt E. Coordinated Ribosomal L4 Protein Assembly into the Pre-Ribosome Is Regulated by Its Eukaryote-Specific Extension. Mol Cell. 2015;58:854-62 pubmed publisher
..We propose that hierarchical ribosome assembly can be achieved by eukaryotic RP extensions and dedicated assembly chaperones. ..