Viviana Gradinaru


Affiliation: California Institute of Technology
Country: USA


  1. Bedbrook C, Deverman B, Gradinaru V. Viral Strategies for Targeting the Central and Peripheral Nervous Systems. Annu Rev Neurosci. 2018;41:323-348 pubmed publisher
  2. Gradinaru V, Treweek J, Overton K, Deisseroth K. Hydrogel-Tissue Chemistry: Principles and Applications. Annu Rev Biophys. 2018;47:355-376 pubmed publisher
  3. Depas W, Starwalt Lee R, Van Sambeek L, Ravindra Kumar S, Gradinaru V, Newman D. Exposing the Three-Dimensional Biogeography and Metabolic States of Pathogens in Cystic Fibrosis Sputum via Hydrogel Embedding, Clearing, and rRNA Labeling. MBio. 2016;7: pubmed publisher
    ..More broadly, MiPACT, coupled with fluorescent labeling, promises to advance the direct study of microbial communities in diverse environments, including microbial habitats within mammalian systems. ..
  4. Rajendran P, Challis R, Fowlkes C, Hanna P, Tompkins J, Jordan M, et al. Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies. Nat Commun. 2019;10:1944 pubmed publisher
    ..Our findings provide new insights into neural regulation of heart rate, and our methodology to study cardiac circuits can be readily used to interrogate neural control of other visceral organs. ..
  5. Challis R, Ravindra Kumar S, Chan K, Challis C, Beadle K, Jang M, et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019;14:379-414 pubmed publisher
    ..The procedures outlined here are adaptable to diverse biomedical applications, from anatomical and functional mapping to gene expression, silencing, and editing. ..
  6. Yang B, Treweek J, Kulkarni R, Deverman B, Chen C, Lubeck E, et al. Single-cell phenotyping within transparent intact tissue through whole-body clearing. Cell. 2014;158:945-958 pubmed publisher
    ..These methods are applicable for high-resolution, high-content mapping and phenotyping of normal and pathological elements within intact organs and bodies. ..
  7. Bedbrook C, Kato M, Ravindra Kumar S, Lakshmanan A, Nath R, Sun F, et al. Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo. Chem Biol. 2015;22:1108-21 pubmed publisher
    ..We have used the binding pair to develop a channelrhodopsin membrane localization assay that is amenable to high-throughput screening for opsin discovery and engineering. ..
  8. Xiao C, Cho J, Zhou C, Treweek J, Chan K, McKinney S, et al. Cholinergic Mesopontine Signals Govern Locomotion and Reward through Dissociable Midbrain Pathways. Neuron. 2016;90:333-47 pubmed publisher
    ..Therefore, the selective targeting of projection-specific mesopontine cholinergic pathways may offer increased benefit in treating movement and addiction disorders. ..
  9. TREWEEK J, Gradinaru V. Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors. Curr Opin Biotechnol. 2016;40:193-207 pubmed publisher
    ..Emerging breakthroughs in viral vector engineering, genome editing tools, and tissue clearing can render larger opaque organisms genetically tractable and transparent for whole-organ cell phenotyping, tract tracing and imaging at depth. ..

More Information


  1. Greenbaum A, Chan K, Dobreva T, Brown D, Balani D, Boyce R, et al. Bone CLARITY: Clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow. Sci Transl Med. 2017;9: pubmed publisher
    ..Bone tissue clearing is able to provide fast and comprehensive visualization of biological processes in intact bone tissue. ..
  2. Cho J, Treweek J, Robinson J, Xiao C, Bremner L, Greenbaum A, et al. Dorsal Raphe Dopamine Neurons Modulate Arousal and Promote Wakefulness by Salient Stimuli. Neuron. 2017;94:1205-1219.e8 pubmed publisher
  3. Robinson J, Gradinaru V. Dopaminergic dysfunction in neurodevelopmental disorders: recent advances and synergistic technologies to aid basic research. Curr Opin Neurobiol. 2018;48:17-29 pubmed publisher
    ..Here, we will review recent findings exploring dopaminergic function in NDD models and discuss the promise of new tools to probe NDD pathophysiology in these circuits. ..
  4. Greenbaum A, Jang M, Challis C, Gradinaru V. Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?. BMC Biol. 2017;15:87 pubmed publisher
    ..In this Q&A, we delineate recent advances and practical challenges associated with these two techniques when applied body-wide. ..