Raymond Deshaies


Affiliation: California Institute of Technology
Country: USA


  1. Li J, Zhang Y, Da Silva Sil Dos Santos B, Wang F, Ma Y, Pérez C, et al. Epidithiodiketopiperazines Inhibit Protein Degradation by Targeting Proteasome Deubiquitinase Rpn11. Cell Chem Biol. 2018;25:1350-1358.e9 pubmed publisher
    ..SOP11 represents a class of Rpn11 inhibitor and provides an alternative route to develop proteasome inhibitors. ..
  2. Deshaies R, Joazeiro C. RING domain E3 ubiquitin ligases. Annu Rev Biochem. 2009;78:399-434 pubmed publisher
    ..Despite their critical importance, our knowledge of the physiological partners, biological functions, substrates, and mechanism of action for most RING E3s remains at a rudimentary stage. ..
  3. Kleiger G, Deshaies R. Tag Team Ubiquitin Ligases. Cell. 2016;166:1080-1081 pubmed publisher
    ..In this issue, Scott et al. show that CRLs activate the RBR enzyme ARIH1 to initiate ubiquitin chains on CRL substrates, thereby marking an unexpected and important advance in our understanding of both enzymes. ..
  4. Liu X, Reitsma J, Mamrosh J, Zhang Y, Straube R, Deshaies R. Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Mol Cell. 2018;69:773-786.e6 pubmed publisher
  5. Deshaies R. Proteotoxic crisis, the ubiquitin-proteasome system, and cancer therapy. BMC Biol. 2014;12:94 pubmed publisher
  6. Sung M, Reitsma J, Sweredoski M, Hess S, Deshaies R. Ribosomal proteins produced in excess are degraded by the ubiquitin-proteasome system. Mol Biol Cell. 2016;27:2642-52 pubmed publisher
    ..Our studies reveal that ribosomal proteins that fail to assemble into ribosomes are rapidly distinguished from their assembled counterparts and ubiquitinated and degraded within the nuclear compartment. ..
  7. Xue L, Blythe E, Freiberger E, Mamrosh J, Hebert A, Reitsma J, et al. Valosin-containing protein (VCP)-Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor. Mol Cell Proteomics. 2016;15:2970-86 pubmed publisher
    ..These findings have significant implications for both the regulation of VCP function and the impact of VCP inhibition on different VCP-adaptor complexes. ..
  8. Cope G, Deshaies R. Targeted silencing of Jab1/Csn5 in human cells downregulates SCF activity through reduction of F-box protein levels. BMC Biochem. 2006;7:1 pubmed
    ..We propose that deneddylation of Cul1 is required to sustain optimal activity of SCF ubiquitin ligases by repressing 'autoubiquitination' of F-box proteins within SCF complexes, thereby rescuing them from premature degradation. ..
  9. Goh A, Walters K, Elsasser S, Verma R, Deshaies R, Finley D, et al. Components of the ubiquitin-proteasome pathway compete for surfaces on Rad23 family proteins. BMC Biochem. 2008;9:4 pubmed publisher
    ..We conclude that the competition of ubiquitin-proteasome pathway components for surfaces on Rad23 is important for the role of the Rad23 family proteins in proteasomal targeting. ..

More Information


  1. Deshaies R, Emberley E, Saha A. Control of cullin-ring ubiquitin ligase activity by nedd8. Subcell Biochem. 2010;54:41-56 pubmed publisher
    ..Here, we review how the Nedd8 cycle controls CRL activity and how perturbations of this cycle can lead to disease. ..
  2. Nguyen T, Lee J, Sweredoski M, Yang S, Jeon S, Harrison J, et al. Glutamine Triggers Acetylation-Dependent Degradation of Glutamine Synthetase via the Thalidomide Receptor Cereblon. Mol Cell. 2016;61:809-20 pubmed publisher
    ..These findings reveal a feedback loop involving CRL4(CRBN) that adjusts GS protein levels in response to glutamine and uncover a new function for lysine acetylation. ..
  3. Nguyen T, Li J, Lu C, Mamrosh J, Lu G, Cathers B, et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc Natl Acad Sci U S A. 2017;114:3565-3571 pubmed publisher
    ..Together, these data point to an unexpectedly intimate relationship between the E3 ubiquitin ligase CRL4CRBN and p97 pathways. ..
  4. Blythe E, Olson K, Chau V, Deshaies R. Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP•NPLOC4•UFD1L is enhanced by a mutation that causes multisystem proteinopathy. Proc Natl Acad Sci U S A. 2017;114:E4380-E4388 pubmed publisher
    ..This work overcomes a significant barrier in the study of p97 and will allow the future dissection of p97 mechanism at a level of detail previously unattainable. ..
  5. Reitsma J, Liu X, Reichermeier K, Moradian A, Sweredoski M, Hess S, et al. Composition and Regulation of the Cellular Repertoire of SCF Ubiquitin Ligases. Cell. 2017;171:1326-1339.e14 pubmed publisher
    ..We show that Nedd8 conjugation and the SR exchange factor Cand1 have a profound effect on shaping the SCFome. Together, these factors enable rapid remodeling of SCF complexes to promote biased assembly of SR modules bound to substrate. ..